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Offlinecheesie
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2nd agar attempt... contam?
    #22272561 - 09/22/15 03:52 AM (8 years, 4 months ago)

This is my second agar attempt, is there contam or anything salvageable?




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Invisiblestareatclouds
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Re: 2nd agar attempt... contam? [Re: cheesie]
    #22272568 - 09/22/15 03:55 AM (8 years, 4 months ago)

Oh god, get rid of that ASAP.


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Offlinecheesie
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Re: 2nd agar attempt... contam? [Re: stareatclouds]
    #22272573 - 09/22/15 03:57 AM (8 years, 4 months ago)

All of it?


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InvisibleLocN9ne
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Re: 2nd agar attempt... contam? [Re: stareatclouds]
    #22272576 - 09/22/15 03:57 AM (8 years, 4 months ago)

:whathesaid:
Lord have mercy.


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Offlinecheesie
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Re: 2nd agar attempt... contam? [Re: LocN9ne]
    #22272577 - 09/22/15 03:58 AM (8 years, 4 months ago)

All contaminated then?


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OfflineMachiavelliavore
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Re: 2nd agar attempt... contam? [Re: LocN9ne]
    #22272581 - 09/22/15 03:59 AM (8 years, 4 months ago)

I think that was on an episode of next generation.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisiblestareatclouds
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Re: 2nd agar attempt... contam? [Re: cheesie]
    #22272588 - 09/22/15 04:02 AM (8 years, 4 months ago)

Quote:

cheesie said:
All contaminated then?




Yeah, bro. I honestly thought this might've been a troll post.


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Offlinecheesie
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Re: 2nd agar attempt... contam? [Re: Machiavelliavore]
    #22272589 - 09/22/15 04:02 AM (8 years, 4 months ago)

So I'm. Dumping all of them? Yes?


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Offlinecheesie
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Re: 2nd agar attempt... contam? [Re: cheesie]
    #22272594 - 09/22/15 04:03 AM (8 years, 4 months ago)

Back to the drawing board then :frown:


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Invisiblestareatclouds
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Re: 2nd agar attempt... contam? [Re: cheesie]
    #22272597 - 09/22/15 04:04 AM (8 years, 4 months ago)

If they all look like that, yes, get rid of them all as safe as possible. You should definitely rethink your agar work and double down on sterility.


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Offlinecheesie
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Re: 2nd agar attempt... contam? [Re: stareatclouds]
    #22272638 - 09/22/15 04:51 AM (8 years, 4 months ago)

Quote:

stareatclouds said:
If they all look like that, yes, get rid of them all as safe as possible. You should definitely rethink your agar work and double down on sterility.





I know the agar and pc'ing is good as I have plates from the batch that haven't contaminated so it's the point of inoculation or the print which is the issue


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Invisiblestareatclouds
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Re: 2nd agar attempt... contam? [Re: cheesie]
    #22272655 - 09/22/15 05:05 AM (8 years, 4 months ago)

I'm not sure, dude. I don't get contams that much and I've never let a plate get that bad before ditching it. Are you using vendor bought spores or what?


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Offlinecheesie
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Re: 2nd agar attempt... contam? [Re: stareatclouds]
    #22272658 - 09/22/15 05:09 AM (8 years, 4 months ago)

To be fair I was on hols for two weeks that's the reason they got so bad


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OfflineRoman08640
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Re: 2nd agar attempt... contam? [Re: cheesie]
    #22272663 - 09/22/15 05:12 AM (8 years, 4 months ago)

yea def contam. with some serious shit lol

if you have a control dish keep a close eye on it to pinpoint contam entry. good luck on your next one!


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Offlinecheesie
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Re: 2nd agar attempt... contam? [Re: Roman08640]
    #22272665 - 09/22/15 05:13 AM (8 years, 4 months ago)

What contam was it?


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