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Offlineluigi_bro
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About Pf Tek- Need some help!
    #22272406 - 09/22/15 01:54 AM (8 years, 4 months ago)

First thing first. Hello guys. I'm a newbie grower. I done my first cultivation process about 2 weeks ago. I don't able to find %100 fitting equipment so i decided with which i can find. I used 350cc jars for colonization.I made 10 jars.After few days they start looking like blurry white and at this process some of them grow green silks and i ended up with 5 jar. After than they start growing things look like spider web with very strong white color but now they stop growing . All jars look blurry white and some areas look like bone. I don't think they are ready for birth but also they stop spreading. Some bottoms are still just look like normal vermiculite and bone look a like areas is just about 1 inch areas also they spreaded all over the jars but they not connected with each other they are separated and also 2 of my jars have about 1.5 inch looks like just wet vermiculite areas at the sides. I recently start getting water drop shaped things in jar walls. I done basic pf tek process with vermiculite and rice flour. I added dry vermiculite at the top. And i kept them in the dark box with bunch off holes and wet perlite.I believe they have enough humidity and temperature always about 30 degree. What should i do with them i need some help from you master growers. Sorry for the long post.


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OfflineMachiavelliavore
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Re: About Pf Tek- Need some help! [Re: luigi_bro]
    #22272431 - 09/22/15 02:15 AM (8 years, 4 months ago)

Kinda confusing post, but I'll say what I can.

My guess is what you described as bone is mush mycellium.  It will start out looking either grey or white, and always grows radially away from the substatte, fist in miniscule fibers, then in expanding radial patches.  As it thickens, it will look whiteish and SOLID.

Anything that appears milky, grey, green, or chaotic/lighteninglike/random is a contaminant mold.  The wet areas may be bacteria.  Look for milky brown/caramel looking fluid.




The perlite chamber with holes you describe, the shotgun fruiting chamber, is for placing the fully colonized cakes in for fruiting, not the jars while they colonize.  The jars can be anywhere when colonizing.  Just try to keep them somewhere not too hot.

Jar size is should be fine.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlineluigi_bro
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Re: About Pf Tek- Need some help! [Re: Machiavelliavore]
    #22272450 - 09/22/15 02:28 AM (8 years, 4 months ago)

I know i describe it like fruting chember but the reason i doing this way is this room is very bright but for tempreture and humidty its the only room i can do this. I placed it inside box because i read somewhere my jars should be out of light. What should i do if the area is get bacteria? I know how to spot contamine areas but i don't saw anything about bacterias. I know my post kinda confusing but i don't know a lot about this things. Yes by bone i mean mycelium.Not strong blurry all over my jars and some spots looks nice and solid. I don't have any photo material now so i can't show but looks like this.


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OfflineMachiavelliavore
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Re: About Pf Tek- Need some help! [Re: luigi_bro]
    #22272575 - 09/22/15 03:57 AM (8 years, 4 months ago)

To answer what I can.

Light is fine.  Don't leave it outside in the sun or it will probably overheat, or in a bright windowsill.  Otherwise irrelevant.  Anyway, nothing wrong with putting them in the sgfc, but you don't have to.

Bacteria, there's nothing you can do about it, or any other contam really.  If you try to take the cake out to remove it early, you contam the whole thing.  All you can really do with any contam is let the mush mycellium take over as much as it can, then try to cut out the contaminant.

Regarding your diagram, generally what you see with a PF cake is 4 expanding regions of intense growth on the sides.  The intense bits look right, but usually it doesn't grow everywhere at once.  You get a burst where the spores were deposited, and then it creeps accross the rest.  Whether you have a contam or not, there's nothing you can do but watch and then try to make sense of what you saw from the final results.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlineluigi_bro
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Re: About Pf Tek- Need some help! [Re: Machiavelliavore]
    #22272606 - 09/22/15 04:13 AM (8 years, 4 months ago)

So is there any solution for getting things speed up? I roam through all topics i can find and some people saying things about shaking jar for air circulation to make un-colonized areas colonized also others says it will harm product and create contam risk. i will do dunk and roll tech. so how long i should wait? its already been 15 days. should cakes must be all solid white? or do you have any advice about other techs? With my description of my jars which method will be more functional? I can sent pictures 6-7 hours later if you wanna see.By the way thanks for your reply and time.


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OfflineMachiavelliavore
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Re: About Pf Tek- Need some help! [Re: luigi_bro]
    #22272622 - 09/22/15 04:35 AM (8 years, 4 months ago)

There is literally nothing you can do to cakes to make them go faster from this point basides keeping them at optimal temperature.  Don't remember what that is cause idgaf, but probably something like 65-75F.

Generally speaking cakes cannot be shaken, since that would disrupt the vermiculite barrier ontop which prevents contaminants from reaching the substrate.  Only cakes that are made using injection ports or opening the lid in a still air box can be shaken.

Wait about 7 days after full colonization to dunk and roll.

As for better teks, basically all of them require a pressure cooker.  I don't know if you have one.  As far as the best you can do without a PC, PF tek is it.  You can improve it by using silicon injection ports, a still air box, and perhaps microwave agar methods in the still air box (SAB.)  Agar will allow you to make sure you have a clean inoculant.  Then you grint it up in a sterilized blender container to make Liquid Inoculant, or cut a wedge and place it in some liquid culture for inoculant.  You can also clone fruits with this method for more consistent results.

Agar is a seaweed gelatin, which is mixed with nutrients + water and sterilized.  Mycellium grows on it in 2 dimensions on thin layers of this moist rubbery plate, allowing easy identification and separation from contaminants.

If you do have a pressure, colonized grains spawned to coco coir bulk substrate in mini monotubs or MSG's would be my recommendation for an easy way to grab a few zips.

Detail the equipment you have and/or how much you're willing to spend and I can refer you to some specific guides.

As far as time frame, it really depends when your spores germinated.  Typically germination takes 6-8 days, but ranges from 2 days to 3 weeks at the very outside.  I think it typically takes like ~3 weeks from inoculation to full colonization, given a week to germination.  It will vary depending on variety, luck, temperature, substrate preparation, etc.


Edited by Machiavelliavore (09/22/15 04:38 AM)


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Offlineluigi_bro
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Re: About Pf Tek- Need some help! [Re: Machiavelliavore]
    #22272637 - 09/22/15 04:49 AM (8 years, 4 months ago)

I don't have pc, or microvawe. I done it with normal pot. microvawe not common at our country it don't be needed too much :smile: Like i said i place them in some black box with bunch of holes for air circulation and i place the box my "special" room with some equipments i used for another things. because this room always set with 85 F. But i take your advice and replace them at another room for try my luck. If i sent pictures tonight can you look them and tell me if look a like wet vermiculite area contam. or not? And sorry for my English if its hard to understand. Thank you again.


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OfflineMachiavelliavore
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Re: About Pf Tek- Need some help! [Re: luigi_bro]
    #22272717 - 09/22/15 05:47 AM (8 years, 4 months ago)

85F will not slow your growth to my knowledge, but it's considerably more beneficial to contaminants than it is to mushroom mycellium.  If it's inconvenient/dangerous to take it out of that room, then you don't have to.  Most likely any damage the increased temperature may have caused has already been done.

Given a regular pot, what you're doing is about the best technique you can do.  You can increase your success rate and make your own cleanish spore prints --> syringes if you make a still air box.  Typically they are made out of clear plastic storage containers, but you could make one out of a regular cardboard box with trash bag stapled over the inside.  If interested in that I can detail out a method that makes sense to me.  Silicon injection ports would also be a nice upgrade.  All you need for that is some silicon sealant and a bit of tyvek/polyfill (synthetic pillow stuffing/filter material.  Also, try to avoid using tall jars, as they are more difficult to inoculate well.

Props to you for speaking another language.  It's a hell of a lot more understandable for you to ask a few questions that could be answered by searches than native english speakers since you're trying to navegate the internet and read guides in a foreign language.

Americans... :notcoolman:

We're really good at something: that's what you learn in college.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlineluigi_bro
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Re: About Pf Tek- Need some help! [Re: Machiavelliavore]
    #22272817 - 09/22/15 06:50 AM (8 years, 4 months ago)

Of course I'am interested with anything will help me. i was thinking about buying clear plastic storage container for let light inside and drill it from all sides then place few inch wet perlite etc. I don't know full method yet but i was seen it somewhere here before. If it will work same i can use cardboard to avoid expenses this time. can you give me more detail please? And about silicone injection ports, i couldn't able to find proper widemouth jars yet when birth time is came I'm just gonna break jars, take the cake and throw the rest. just used some kind of medical tape allowing air circulation for cover syringe holes. Next time i will buy right equipment's online and do it like you said. Since beginning i know i have some wrongs like tall jars but i was in a rush and do best i can do in mean time. I just done most suitable option form me.But when this situation arrive i get little worried and decided ask someone.


Edited by luigi_bro (09/22/15 07:02 AM)


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OfflineMachiavelliavore
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Re: About Pf Tek- Need some help! [Re: luigi_bro]
    #22275038 - 09/22/15 04:38 PM (8 years, 4 months ago)

Quote:

luigi_bro said:
Of course I'am interested with anything will help me. i was thinking about buying clear plastic storage container for let light inside and drill it from all sides then place few inch wet perlite etc. I don't know full method yet but i was seen it somewhere here before. If it will work same i can use cardboard to avoid expenses this time. can you give me more detail please? And about silicone injection ports, i couldn't able to find proper widemouth jars yet when birth time is came I'm just gonna break jars, take the cake and throw the rest. just used some kind of medical tape allowing air circulation for cover syringe holes. Next time i will buy right equipment's online and do it like you said. Since beginning i know i have some wrongs like tall jars but i was in a rush and do best i can do in mean time. I just done most suitable option form me.But when this situation arrive i get little worried and decided ask someone.




A still air box is a not a fruiting chamber.  It's a chamber with limited airflow you put your arms into to do sterile work in still air conditions to avoid contaminating open jars.  This can be made with a cardboard box.

The SGFC should be translucent, as light is a secondary pinning trigger.  Also you don't want extreme humidity + cardboard.  Plastic is the way to go here.

Glass Jars are not the only option.  Some plastic food containers work, as long as they are made of Polypropylene.  They are usually marked PE and recycling code 5 here.  You can look at things like dips and hummus that might be packaged in PE, or often times value stores sell them.  Considerably cheaper than jars, and may be easier to find.


Good luck breaking those jars.  You could also try to fruit them without removing them from the jars.  There's a guy on here that just sticks a sandwich bag over the top and gets decent results.  I'd probably skip the fruiting chamber and just do that.  With narrowmouths, sidepinning will be a bitch though.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlineluigi_bro
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Re: About Pf Tek- Need some help! [Re: Machiavelliavore]
    #22282290 - 09/24/15 06:44 AM (8 years, 4 months ago)

Oh ok now i understand. Can you give me more info about still air box please?
And here my jars what do you think about them?



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OfflineMachiavelliavore
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Re: About Pf Tek- Need some help! [Re: luigi_bro]
    #22282399 - 09/24/15 07:27 AM (8 years, 4 months ago)

Looking good to me.  How long have these been going since germination?

That spot on the first pic looks a bit funky.  Can't see quite well enough to say.  Bottom of the 2nd might be okay, might be funky.  Anyway, if they don't colonize just crack'm and then cut it out.


You want a box between like .1 and .2 cubic meters.

Put the box where you want to work.  If you're looking down, cover the top of the box with clear plastic and cut two armholes in the side.  If you're more looking in from the side, place the box sideways, cover clear plastic, then just fold it halfway up when working.  Most likely the former will be likely.  Cover the inside with garbage bags, then tac or staple them on.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlineluigi_bro
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Re: About Pf Tek- Need some help! [Re: Machiavelliavore]
    #22282412 - 09/24/15 07:33 AM (8 years, 4 months ago)

Ok thank you for your advice. First picture is little blurry but its almost same with second one. I made this jars 16 days ago.I took these pictures yesterday.Empty areas looks same since 4th - 5th day.

How long should i wait before birth according to this pictures?


Edited by luigi_bro (09/24/15 07:37 AM)


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OfflineMachiavelliavore
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Re: About Pf Tek- Need some help! [Re: luigi_bro]
    #22282443 - 09/24/15 07:43 AM (8 years, 4 months ago)

Give it some more time.  That's not too long at all.  Just look at the rhizomorphic mycellium near the edges of the uncolonized areas.  Mark their advance with a marker.  As long as it's making progress, keep letting it go.

If there has been no change in the last 10 days, it's probably fine.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlineluigi_bro
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Re: About Pf Tek- Need some help! [Re: Machiavelliavore]
    #22282459 - 09/24/15 07:49 AM (8 years, 4 months ago)

Okey thank you so much.:thumbup:


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