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Offlinefunguy64
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first timer here, a few quick questions
    #22270999 - 09/21/15 07:03 PM (8 years, 4 months ago)

he guys, first post, first run, so cut me some slack here.

I am running the brf pf tek to sgfc, as close to the RR vids as possible.
I have a SAB, and im going to use with the lid on, and work on the inside bottom. will be stearlizing with 90%iso, or bleach (what % to water for that?)
Im going to diy a little burner (kept outside the box of course) for the spore syringe. (what I ordered and whatever they tossed in as a freebie)
Some people here are saying use holes in my jars, some say no holes. Some say tape, other no tape. 4 holes, 1 hole two hole ect. Im a bit overwhelmed on that aspect (I wana do exactly what works, and experiment later on)
ok, so when the jars cool down, they will basically go straight into the sab, and wipe down with 90%iso, do I also wipe the lids?
Lids will be rubber up (1/2pt ball) and I assume on fairly tight, but not stupid tight?
I should then take the foil off, and pump em up with the goods.
Im doing 12 (6 of what I ordered and 6  whatever the other is, 1 jar is a pre sterilized from a vendor here, just to test out my process of getting mine sterile.
So i guess my questions were that,
holes or no holes, and how many. Do I iso wipe them before innoc?
as far as a place to let them do their thing after that, can I just keep in my SAB to reduce contams, or do they need more airflow?

sorry for the jumble of info here, everthing will be here by wed, and doing this thurs or friday.

thoughts, comments, suggestions all appreciated!


Edited by funguy64 (09/21/15 08:55 PM)


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Invisiblesweetpea
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. [Re: funguy64]
    #22271032 - 09/21/15 07:09 PM (8 years, 4 months ago)

.


Edited by sweetpea (10/06/15 12:44 PM)


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Offlinemushpunx
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Re: first timer here, a few quick questions [Re: funguy64]
    #22271041 - 09/21/15 07:11 PM (8 years, 4 months ago)

You don't need to sterilize a still air box, it doesn't work like that. The idea is to keep the air as still as possible so nothing settles into your work. It wont be sterile, you couldnt sterilize it if you wanted to.

You mix up a little dish soapy water in a spray bottle and spritz down the walls, this helps trap airborn contams. 'Some people wipe down the walls with ISO first but I would only wash it out and do this if its like real dirty or something.

You need holes in your jars, for gas exchange and they double as inoculation holes. Use 4 holes.

You also need a dry layer of vermiculite on top of your BRF, this acts as a filter, contam barrier in leu of a proper filter.



You would prep your still air box, wipe down all your jars with ISO before putting them inside (70 percent, not 90). Wash up, wear gloves and slather them up with ISO when you work.

After your jars cool, flame sterilize your syringe and put 1/4 cc aprrox in each hole.



  Its a good idea to use a still air box for innoculating PF jars.


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Edited by mushpunx (09/21/15 07:12 PM)


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: mushpunx]
    #22271074 - 09/21/15 07:18 PM (8 years, 4 months ago)

Do I wipe the holes down?
Ill use a nail and hammer and make those tonight.
gunna use that search bar and figure the size out, dont want them too big!
@mushpunx I have the verm, just forgot to add that, gunna bake that before I put it into my jars as an extra extra precaution.
my brf will also be ground up by me (organic generic brand brown rice?)

@sweetpea Id rather over think this and do everything that is proven on here to work than to mess this up, but point well taken!

thanks for the speedy response guys!!

edit: going with 1/8th inch holes. punching now, wish me luck!


--------------------
:prettyflyforawhiteguy:


Edited by funguy64 (09/21/15 07:24 PM)


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Offlinemushpunx
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Re: first timer here, a few quick questions [Re: funguy64]
    #22271099 - 09/21/15 07:24 PM (8 years, 4 months ago)

I wouldn't bother wiping for just PF jars honestly. Just take them out of the pot when they are cool enough and put them in still air box to finish cooling.

Make sure to flame sterilize needle till red hot betweeen innoculations.

Don't bother baking the verm, you are going to sterilize it when you ster ilize your jars.

You need to mix your BRF and verm and water together in the proper proportions, fill your jars, then fill the last 1/2 inch of each jar with just plain dry verm before you put the lids on, just like RR does in the video.

Just follow the RR videos to a T


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: mushpunx]
    #22271111 - 09/21/15 07:26 PM (8 years, 4 months ago)

yeah ive basically had those vids on a loop, haha.
looks like im doing good so far though, thats good!


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OfflineMachiavelliavore
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Re: first timer here, a few quick questions [Re: funguy64]
    #22272770 - 09/22/15 06:21 AM (8 years, 4 months ago)

Quote:

funguy64 said:
Do I wipe the holes down?
Ill use a nail and hammer and make those tonight.
gunna use that search bar and figure the size out, dont want them too big!
@mushpunx I have the verm, just forgot to add that, gunna bake that before I put it into my jars as an extra extra precaution.
my brf will also be ground up by me (organic generic brand brown rice?)

@sweetpea Id rather over think this and do everything that is proven on here to work than to mess this up, but point well taken!

thanks for the speedy response guys!!

edit: going with 1/8th inch holes. punching now, wish me luck!





Whoah whoah whoah...  Never add a verm layer after sterilization.  That's begging for contams.  You can minimize the chances, but still, opening up jars and pouring shit isn't the best idea.

If you meant you're going to add the verm before you steam/PC them, then there is no need to do anything to it.  It will be sterilized along with your cakes.

Your holes only need to be big enough to fit a needle.  A single hole that size would provide sufficient GE for colonization.

Overall it sounds like you're going overboard with precautions with a tek that was designed to work in open air with minimal sterile technique.  Not to say it's bad to do that, just don't put yourself through the spin cycle worrying.

The main reason for using a still air box is to be able to open grain jars, lcs, and agar plates without contaminating them.  PF tek is injecting through a vermiculite barrier purposed with stopping spores and bacteria with a flame sterilized needle.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (09/22/15 06:26 AM)


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: Machiavelliavore]
    #22274259 - 09/22/15 02:03 PM (8 years, 4 months ago)

i meant that id first bake the verm, add to jars, then sterilize it all.
that seems to be over kill though.
sab is more practice because i want to tale spore prints this grow, transfer to agar, and do a monotub for my round 2.

jars have 4 holes 1/8in. just like the rr video jars.
hopefully my worrying wont stress my fungi out. guess i should just calm down and see what happens.
innoc either tomorrow or friday.
ill keep yall posted!


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:prettyflyforawhiteguy:


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: funguy64]
    #22278566 - 09/23/15 12:29 PM (8 years, 4 months ago)

alright, so i had recieved 2 pre sterilized brf jars from a vendor on here, came with micropore tape on one hole and a blue self healing port for the other.

went ahead and inoculated those today(kinda tonsee if they work and my diy jars dont for contamination reasons)
i did the whole thing in my sab, and they are in there now.
would just in my room be ok, or keep in the box?
i read inoculation chambers werent really necessary, but figured id get yalls opinion.
jars went through the whole sab clean hands process btw.
i assume those self healing ports and micropore filter thing will keep contams out, so is on a shelf in my room ok?
temps there are around 68 to 74 constantly, with natural daylight and not much else lighting.
didnt tape over the self healing port, but it seems pretty well sealed.
thoughts?
the rest of my project will begin this friday, 12 more jars that have no healing ports, but my verm layer should be fine for those right?
thanks guys.


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:prettyflyforawhiteguy:


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OfflineDionili
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Re: first timer here, a few quick questions [Re: funguy64]
    #22278697 - 09/23/15 12:54 PM (8 years, 4 months ago)

keep them off the bottom foot of air space. KEEP ANYTHING YOU NEED CONTAM FREE OFF THE BOTTOM FOOT OF AIR SPACE!


Besides that, room temperature dry place. innoc chambers are not really needed with your temps also keep them out of direct light.


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: Dionili]
    #22278783 - 09/23/15 01:10 PM (8 years, 4 months ago)

they are up about 3 to 4 feet of the ground currently in my sab. will probably move them to a shelf higher up just because.
ac in room, but i guess at this point that doesnt matter, jars should remain steril because of self heal port and the microfilter tape stuff?
sorry for so many noob questions, just wana get this right.


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:prettyflyforawhiteguy:


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OfflineDionili
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Re: first timer here, a few quick questions [Re: funguy64]
    #22278824 - 09/23/15 01:23 PM (8 years, 4 months ago)

Quote:

funguy64 said:
they are up about 3 to 4 feet of the ground currently in my sab. will probably move them to a shelf higher up just because.
ac in room, but i guess at this point that doesnt matter, jars should remain steril because of self heal port and the microfilter tape stuff?
sorry for so many noob questions, just wana get this right.





Yeah, but if theres a ton of contaminants on the outside of the jar it could spoil the fruiting process


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: Dionili]
    #22278891 - 09/23/15 01:37 PM (8 years, 4 months ago)

wiped everything down with iso in the sab before innoc.
how can I prevent contams at this point?
should I cover them or somthing?
now im kind of sweating the whole thing.
as Ive said this is my first time at this, and I want to do it right!

should I leave in the sab, or just see what happens leaving them on the shelf in my room out of the sab?

sorry for so may questions guys, like I keep saying, Im a do it right the first time kind of person, and everyone here knows exactly what works already.


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:prettyflyforawhiteguy:


Edited by funguy64 (09/23/15 02:48 PM)


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Offlinemushpunx
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Re: first timer here, a few quick questions [Re: funguy64]
    #22279593 - 09/23/15 04:33 PM (8 years, 4 months ago)

Just flame sterilize the needle between innoculations, and put them on a shelf or something and leave them alone.

The dry verm layer acts as a filter. The only way they will get contaminated is if 1. You didn't flame the needle till it was red hot between injections and introduced a contaminant that way, 2. The syringe was contaminated or 3. You move them around a lot and knock some of the dry verm layer (that catches contaminants) down into the BRF. Substrate... this is kinda unlikely though.

Its good practice to use a still air box but PF. Tek is designed so it could be done in open air, its pretty hard to fuck up


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Re: first timer here, a few quick questions [Re: mushpunx]
    #22279615 - 09/23/15 04:38 PM (8 years, 4 months ago)

Don't overthink it too much! If you're following RR's video then it's hard to go wrong. Just do what he does! The only thing I would suggest is getting Kerr 1/2 pint wide mouth jars. The ball ones are too tall and will probably stall; I just did 6 of those and it was a pain in the ass


--------------------
:mushroom2: Have a good day :mushroom2::lol:


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: mushpunx]
    #22279665 - 09/23/15 04:49 PM (8 years, 4 months ago)

the two jars I did today were pre sterilized from a seller that has good reviews here.
no verm layer inside the jars, so IDK, but everyone here seems to like the guy.
Ill put them up on my tall shelf and forget about them for awhile.
gunna order some pre sterilized agar media plates (no pc) and try my hand at getting something going with that.
needle was wiped with iso, and then flame sterilized red hot before innoc, and re flamed between the two jars.


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:prettyflyforawhiteguy:


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: blew42]
    #22279670 - 09/23/15 04:51 PM (8 years, 4 months ago)

Quote:

blew42 said:
Don't overthink it too much! If you're following RR's video then it's hard to go wrong. Just do what he does! The only thing I would suggest is getting Kerr 1/2 pint wide mouth jars. The ball ones are too tall and will probably stall; I just did 6 of those and it was a pain in the ass



I will get those for the next round, already have the ball, so Im going to just try with these, as alot of people seemed to say they worked fine, if only taking a bit longer to colonize and consolidate.

thanks for the responces guys, trying not to over think this, im just excited.


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:prettyflyforawhiteguy:


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Re: first timer here, a few quick questions [Re: funguy64]
    #22280078 - 09/23/15 06:35 PM (8 years, 4 months ago)

No verm layer huh? Does it have any filter on top? You either need a filter , or a dry verm barrier, or you will be in danger of contamination


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: mushpunx]
    #22280110 - 09/23/15 06:44 PM (8 years, 4 months ago)

yes, the two that I recieved pre sterilized have self healing injection ports, and some sort of micro pore filter thing on them.
they seem pretty legit, and the person selling them has some pretty good reviews on this site.
those two jars have been inoculated, so Ill keep yall posted on those.
others will be steamed and inoculated later this week.


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Re: first timer here, a few quick questions [Re: funguy64]
    #22280155 - 09/23/15 06:56 PM (8 years, 4 months ago)

Right on, yea those are nice, you can 're use those jars and they will never need dry verm layer


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: mushpunx]
    #22280254 - 09/23/15 07:17 PM (8 years, 4 months ago)

not sure how many times I will trust that self sealing port, but ill probably get a few extras to replace after a few runs with it.


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Invisiblesweetpea
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. [Re: funguy64]
    #22280420 - 09/23/15 08:02 PM (8 years, 4 months ago)

.


Edited by sweetpea (10/06/15 11:06 AM)


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: sweetpea]
    #22280631 - 09/23/15 08:26 PM (8 years, 4 months ago)

true, and i may, but tbh this little blue self healing port was pretty neat.
that said, with a verm layer (which my diy jars will have) it seems a bit overkill...


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OfflineMachiavelliavore
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Re: first timer here, a few quick questions [Re: funguy64]
    #22281596 - 09/23/15 10:47 PM (8 years, 4 months ago)

Just grab a tube for $3 at ace.  If you're ambitious about mycology, you will probably be opening jars in a SAB in no time and giving 0 fux about dealing with making a ton of injection ports and going to the trouble of repeated flaming, schwabbing ports, and injecting.

I usually open the lids even on containers that have old injection ports.  It's just so easy to do extremely clean syringe work with a cracked open lid.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: Machiavelliavore]
    #22282961 - 09/24/15 09:57 AM (8 years, 4 months ago)

probably will.
used my sab this time just for practice.
now that i know i can easily work inside it, while keeping to the back, i am less nervous about agar work.
cant wait to try out making prints and all that such, that is if this run has any sort of success.


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OfflineMachiavelliavore
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Re: first timer here, a few quick questions [Re: funguy64]
    #22284577 - 09/24/15 03:59 PM (8 years, 4 months ago)

I focused on a lot of the wrong things when I started, but shit got way better when I mostly focused on never getting my hands above my works.

Beyond that just try to be smoothe and efficient.  Don't stress, don't hurry.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinefunguy64
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Re: first timer here, a few quick questions [Re: Machiavelliavore]
    #22284978 - 09/24/15 05:23 PM (8 years, 4 months ago)

deff. trying to take my time, especially with reading and understanding the various teks on here.


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:prettyflyforawhiteguy:


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