|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
|
Just had the worst time inoculating
#22270952 - 09/21/15 06:55 PM (8 years, 4 months ago) |
|
|
i just went to inoculate 2 jars in my bleached down small closet, and as i was squirting spores into the 2nd jar my fucking spore syringe jammed with a spore clump! so i had to open the pot i sterilized my jars in and suck a bit of the water from the pot that had been sterilized into the needle in order to un clog the needle tip.. and then i proceeded to accidentally put to much spore water in the 2nd jar ... This was really a bad experience at inoculation. the first time was smooth sailing.
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
|
|
Spore clump? Are you using a 100 gauge needle? You need a 16 gauge.
|
NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
|
Re: Just had the worst time inoculating [Re: YaMoonSun]
#22270985 - 09/21/15 07:01 PM (8 years, 4 months ago) |
|
|
it was a pretty big clump inside the spore syringe, and idk what gauge it is whatever gauge the store sold me.
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
|
|
Theyre generally colour specific
|
NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
|
Re: Just had the worst time inoculating [Re: YaMoonSun]
#22271016 - 09/21/15 07:05 PM (8 years, 4 months ago) |
|
|
its blackish greyish the colour
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
|
Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
|
|
did you give that puppy a good shake before use? that'll break up the big clumps usually.
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
|
|
Says 22 gauge, ask for a 16 gauge next time around.
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
|
Re: Just had the worst time inoculating [Re: YaMoonSun]
#22271076 - 09/21/15 07:18 PM (8 years, 4 months ago) |
|
|
You sucked up water into your syringe?? Dude Im pretty sure that un sterilized it.
Unless you are saying you sterilized water and then sucked some sterile water in, inside a still air box using proper sterile tek
But if sounds like you just sucked up water from the pot you steamed your jars in out in the open .... yikes
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (09/21/15 07:20 PM)
|
NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
|
Re: Just had the worst time inoculating [Re: mushpunx]
#22271098 - 09/21/15 07:24 PM (8 years, 4 months ago) |
|
|
I took the jars out of the pot, closed it and opened it a second time to suck water into the syringe.. Only opened the pot twice after it was. Sterilized and I opened it only in my small bleached down closet, which is my Sab for now
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
|
NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
|
Re: Just had the worst time inoculating [Re: Psilosopherr]
#22271104 - 09/21/15 07:25 PM (8 years, 4 months ago) |
|
|
Quote:
rbalzer said: did you give that puppy a good shake before use? that'll break up the big clumps usually.
Yeah I have shaken it lots of times, for some reason that one clump wouldnt break
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
|
|
Meh, hopefully you will be ok but in the future if it clogs try flaming the needle a couple times instead.
Thats how I used to un clog a needle back when I used to shoot dope.
But yea, I dunno hopefully you will be OK I dunno.. once you opened the pot to take your jars out the water ceased to be sterile.
Oh, and build an actual still air box man, you wont be able to keep the air still enough for sterile work most likely in a bleached closet. Itll work good enough for PF TEK but BRF jars can be inoculated in open air for the most part.
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (09/21/15 07:30 PM)
|
NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
|
Re: Just had the worst time inoculating [Re: mushpunx]
#22271132 - 09/21/15 07:30 PM (8 years, 4 months ago) |
|
|
Man I tried everything lol, flicking it, shaking it , flaming it, and sucking up the water was last resort .
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
|
WolfieNuke
Professional Psychonaut



Registered: 12/21/09
Posts: 421
Loc: Washington, US
Last seen: 6 years, 7 months
|
|
I would swap the needle out before ever sucking any liquid back into it. Go ahead and purchase some spare syringe needles for next time.
Usually a good flame will burn up anything inside and a nice push on the plunger will dislodge it. I've had issues with vermiculite in larger LC syringes, but flaming the needle usually fixes this issue. How much force did you use to try to dislodge the needle? Sometimes it can take some force...
Whatever water you sucked into the syringe is probably suspect, even if you opened the lid once.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Just had the worst time inoculating [Re: WolfieNuke]
#22271325 - 09/21/15 08:09 PM (8 years, 4 months ago) |
|
|
that was probably verm/flour that clogged it, flame it next time
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
|
Re: Just had the worst time inoculating [Re: cronicr]
#22271348 - 09/21/15 08:13 PM (8 years, 4 months ago) |
|
|
It was infact not the vermiculite or rice flour, it was a huge clump of spores that wouldn't break, I could literally see the clump of spores clogging up the spot right before the needle tip, nothing else worked other than sucking water up, oh well fuck it.. I have 4 jars that are colonizing with no contams I'll just buy a fucking new syringe smfh
Edited by NewToCultivation (09/21/15 08:14 PM)
|
Shroomsh
Mad King



Registered: 07/28/15
Posts: 121
Loc: Washington
Last seen: 7 years, 3 months
|
|
Well that...sucks.
|
Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
|
Re: Just had the worst time inoculating [Re: Shroomsh]
#22271966 - 09/21/15 10:39 PM (8 years, 4 months ago) |
|
|
flame needle and suck sterile hot air in, then you can reshake without worry.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
|
NewToCultivation
New Cultivator



Registered: 08/31/15
Posts: 185
|
Re: Just had the worst time inoculating [Re: Sksoul]
#22272805 - 09/22/15 06:43 AM (8 years, 4 months ago) |
|
|
I tossed out the spore water last night, took it apart and soaked it in rubbing alcohol over night... I plan to use it for a spore syringe when my fruits develope in the near future. in the meantime I am going to buy a new one this weekend . Hopefully get a pan cyan syringe
-------------------- everything I do or say is for informational purposes only I do not condone the cultivation or sales of any illegal substances. Oh yeah, and i fucked bodhisatta's mother.
Edited by NewToCultivation (09/22/15 06:44 AM)
|
YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
|
|
Try steam sterilization instead, wrap the syringe in aluminum foil and toss it into a preesure cooker or closed-lid pot.
|
TrichFarmer
Stranger

Registered: 09/21/15
Posts: 5
Last seen: 7 years, 10 months
|
Re: Just had the worst time inoculating [Re: YaMoonSun]
#22273020 - 09/22/15 08:28 AM (8 years, 4 months ago) |
|
|
If the clumps don't come apart in the syringe I just leave them there. there's enough spores in the water to inoculate as long as you shook it. They usually colonize much faster at that specific point although it's probably a clusterfuck of genetics. Make sure to take note of the jar with the clump in it cause the black dot usually looks like a possible contam.
|
|