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Offlineaerow.thefox
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Re: aerows first agar [Re: iSmkGrnBud]
    #22298517 - 09/27/15 01:05 PM (8 years, 4 months ago)

took my transfer yesterday. now we wait. :fishing:


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Offlineaerow.thefox
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Re: aerows first agar [Re: aerow.thefox]
    #22313141 - 09/30/15 09:31 AM (8 years, 3 months ago)

update: the red boy slice is now totally fuzzy and jumping on to the agar. id say a couple more days and itll be ready.

in addition to that i recieved a syringe of pan cyan that i put to agar as well. my problem is that the mycelium thats popping up has a bald spot in the middle of the germination points. normal or no?


edit: this is my first pan cyan grow. the tissue around it looks like the fluffy white mycelium i see in pics. just the bald spot seems weird..


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Edited by aerow.thefox (09/30/15 10:59 AM)


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Offlineaerow.thefox
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Re: aerows first agar [Re: aerow.thefox]
    #22319039 - 10/01/15 12:53 PM (8 years, 3 months ago)

so my RB plate is starting to take off again. its not showing any strong rhizo growth but its plenty clean now. i dont see any contams and iv been checking all the plates iv transfered from and no contams. my original T0 dish is actually totally colonized.

so what would you say my next step should be. continue transfers till strong rhizo growth?

or straight to tiger drop plates?

i intend to get a good clone culture to work with. so im not sure if i want to limit genetics much more. advice?


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Offlineaerow.thefox
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Registered: 06/10/10
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Re: aerows first agar [Re: aerow.thefox]
    #22379598 - 10/14/15 04:38 PM (8 years, 3 months ago)

i have been very lax on my updates but progress on RB has been slow which was surprising. seemed as though it was much faster in the beginning. but the plates i used were a couple weeks old so maybe they were dry? i dont know. but the RB-t3 didnt look to good. it didnt look like contams but the mycelium was very fluffy and airy most of the way around. i found one good rhizo sector and took a xfer of it to some newly cooked plates.

my pans however seem to be doing just fine on the plates. took another strong sector and transfered to t3


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