Home | Community | Message Board

Avalon Magic Plants
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Cultivation Supplies   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
aerows first agar
    #22268759 - 09/21/15 10:41 AM (8 years, 4 months ago)

hey guys!! so i won a red boy print from stropharis a little while ago. so i made some of my first agar dishes (pastywhites no pour) made sure my ratios were right. that it was the right consistancy. then made some more and transfered some spores to the plate using a sterilized (in pc) qtip, on 9/14. today i finally see growth.

so my first mistake was using quilted 1/4 pints. i wanna use the 1/4s cuz i can re-use em for mini cakes. so yesterday i went and got some more 1/4 pints (the unquilted kind) and some pp5 mini-rounds and made up little more agar.

what i wanna know is whether its time for a transfer. its hard to tell with the quilting. i couldnt get you a pic without removing the lid. i placed the spores a little off center, but the white growth is directly in the middle just off the side of the spore load, and fuzzy, not rhizo. i also see a couple white spots in other places away from the spore load. so im a little worried. although spores could have fallen off the qtip and floated to other areas i suppose..

so what do you think. at least do a transfer to get it to a more visible container(and pics for you since ill be taking the lid off)?


--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: aerows first agar [Re: aerow.thefox]
    #22268791 - 09/21/15 10:48 AM (8 years, 4 months ago)

Without pictures it's hard to say..

Do you have a SAB? Open it up in the SAB & take pictures.


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: Fantastic Mr. Fox]
    #22268797 - 09/21/15 10:49 AM (8 years, 4 months ago)

will do. gimme a min


--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: aerows first agar [Re: aerow.thefox]
    #22268806 - 09/21/15 10:51 AM (8 years, 4 months ago)

Dont put the camera in there though lol


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


Extras: Filter Print Post Top
OfflineiSmkGrnBud
Psychonaut
 User Gallery


Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 4 hours
Re: aerows first agar [Re: aerow.thefox]
    #22268810 - 09/21/15 10:52 AM (8 years, 4 months ago)

Pics :popcorn:

Yea those quilted side jars suck sometimes when you're trying to distinguish what's going on. I usually will let my transfers grow until they're a little larger than a quarter, or unless I see a contam I can get away from.


--------------------
:bigweed:  iSmkGrnBud's Teks  :bigweed:
:mushroomgrow: iSmkGrnBud's Wild Mushroom Finds :mushroomgrow:
:amanita2: The Noob Forum's List of Teks :amanita2:

“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: iSmkGrnBud]
    #22269050 - 09/21/15 11:46 AM (8 years, 4 months ago)



edit: ill try to get a more clear one but its hard. tubs hard to take pics through lol


--------------------
need a pc build list? i got you



Edited by aerow.thefox (09/21/15 11:47 AM)


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: aerow.thefox]
    #22269069 - 09/21/15 11:54 AM (8 years, 4 months ago)


i cant get the camera to focus on the jar very well. it tries to focus on the SAB. so frustrating.. :mad2:


--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: aerow.thefox]
    #22269071 - 09/21/15 11:55 AM (8 years, 4 months ago)

i feel like that outer thin layer might be contam. what do you guys think?


--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 14 hours
Trusted Cultivator
Re: aerows first agar [Re: aerow.thefox]
    #22269108 - 09/21/15 12:06 PM (8 years, 4 months ago)

Yea man take a transfer. On first plate you want to take a transfer as soon as you see growth big enough to take one really, I usually do when growth gets around the size of a dime tops.

Don't worry if its fuzzy or rhizo, just take something that looks healthy to your eyes. THe first plate from spores is usually really fuzzed out and hard to tell whats what anyways so just take a piece that looks healthy and clean  and is furthest away from any bacteria or mold colonies.


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: mushpunx]
    #22269183 - 09/21/15 12:30 PM (8 years, 4 months ago)

what are your favorite colors for agar? iv tried this blue and a purple that turned out more pink. i like blue more.


--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 14 hours
Trusted Cultivator
Re: aerows first agar [Re: aerow.thefox]
    #22269197 - 09/21/15 12:34 PM (8 years, 4 months ago)

Ive never used color in my agar actually so I like to get mine as clear as I can. But I might start using it to distinguish between different recipies/antibiotic agar and what not


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: mushpunx]
    #22269484 - 09/21/15 01:45 PM (8 years, 4 months ago)

i tried to post a vid of the transfer but i cant get it to load an attachment.
edit: ooooh my attatchent is too big


--------------------
need a pc build list? i got you



Edited by aerow.thefox (09/21/15 01:59 PM)


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: aerow.thefox]
    #22278570 - 09/23/15 12:30 PM (8 years, 4 months ago)

well i dont think it matters much but heres an update on the first dish after it grew out some. kept it to check up on my sterile procedure after the first transfer.  i dont see any contam. i kno its a little blurry. but i think i did okay. how long would it take for them to show if my procedure contaminated it?


and heres plate #2. the recipient of the first transfer.



--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 14 hours
Trusted Cultivator
Re: aerows first agar [Re: aerow.thefox]
    #22279575 - 09/23/15 04:27 PM (8 years, 4 months ago)

No way to know really, just let the second plate grow out some... like the size of a nickel or a quarter (unless you see it growing towards bacteria or mold.. then take a transfer asap).. and try to take another transfer from the leading edge of the healthiest section :thumbup:


You're doing fine man


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: mushpunx]
    #22281414 - 09/23/15 09:53 PM (8 years, 4 months ago)

thx punx. :smile:


--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: aerow.thefox]
    #22286393 - 09/24/15 10:29 PM (8 years, 4 months ago)

just wanna say iv used agar for all of 6-7 days and i already love the stuff. i had an lc that i did some noccing with and they all came out bad.(b4 i had the agar) so 2 days ago i put a little squirt on some agar to see what was goin on. apparently ALL the white growth suspended in that lc is trich. :uhoh: not a lick of healthy nada.

so yea agar ftw buuuuuudy.

p.s. THX PASTY!!!!


--------------------
need a pc build list? i got you



Edited by aerow.thefox (09/24/15 10:45 PM)


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 14 hours
Trusted Cultivator
Re: aerows first agar [Re: aerow.thefox]
    #22287792 - 09/25/15 10:23 AM (8 years, 4 months ago)

Dude agar is such a good tool. This is when the fun starts man the good stuff
wait till you fruit your first clones or see your first monoculture!


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: mushpunx]
    #22288002 - 09/25/15 11:32 AM (8 years, 4 months ago)

i know i cant wait!!! especially when these boogers show up in the mail.
:vaped:

but on to a little more on track discussion. heres an update of transfer 1 after 4 days of growth.


edit: stupid question


--------------------
need a pc build list? i got you



Edited by aerow.thefox (09/25/15 12:13 PM)


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: aerows first agar [Re: aerow.thefox]
    #22292960 - 09/26/15 11:15 AM (8 years, 4 months ago)

update: the growth took off a bit from yesterday and is about the size of a nickel. one side has definetly grown a little faster/farther. how long to let it go before should i take another transfer?

btw id like to make and test some isolates to find a good fruiter. thats what this thread is planned to develope into. but until then id just like to have something clean to work with.


--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
OfflineiSmkGrnBud
Psychonaut
 User Gallery


Registered: 10/28/11
Posts: 891
Loc: Your subconscious
Last seen: 11 days, 4 hours
Re: aerows first agar [Re: aerow.thefox]
    #22292986 - 09/26/15 11:21 AM (8 years, 4 months ago)

I'd say transfer now. :cheers:


--------------------
:bigweed:  iSmkGrnBud's Teks  :bigweed:
:mushroomgrow: iSmkGrnBud's Wild Mushroom Finds :mushroomgrow:
:amanita2: The Noob Forum's List of Teks :amanita2:

“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Cultivation Supplies   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* agar transfer substitution? omniscientlore 1,478 3 12/19/22 07:14 AM
by Screwup
* How to make agar 2 agar transfer? esp 6,655 7 01/08/04 11:08 AM
by YidakiMan
* agar to agar transfer ohmatic 15,622 11 01/12/21 09:00 PM
by bodhisatta
* agar transfers *DELETED* thisone 1,245 3 11/20/03 08:58 PM
by Mycomancer
* substitute for agar? brewwhaha1 2,257 15 02/19/05 09:27 PM
by MontyRev
* agar to agar / g2g degeneration effects ohmatic 2,621 11 01/17/05 03:46 PM
by MushroomFriend
* Innoculation with agar wedge Visigoth 5,278 6 01/15/23 12:59 PM
by Screwup
* Culture Transfer to Liquid actionshroom 786 1 04/09/05 02:39 PM
by mattymonkey

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,529 topic views. 34 members, 195 guests and 46 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.011 seconds on 16 queries.