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kellogg
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Registered: 07/05/13
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do you think its prone to contam?
#22268681 - 09/21/15 10:16 AM (8 years, 4 months ago) |
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okay so I've done a few grows but im still learning. just jumped into bulk(ish) after 3 brf and 2 wbs successful grows. i say "ish" because i decided to be careful, and only spawned about a pint and a half of wbs to some coir. idk like to hear peoples input on whether or not i screwed up. i just finished waiting about a week for my mini tray to colonize, it was basically 100% except two spots of coir less than a thumbtack small left uncolonized. i proceeded to load my tray and two more pints of uncolonized, sterilized wbs into my SAB, and dumped the wbs onto the surface of my tray, no breaking up of the tray or anything, just straight poured. i bleached the inside of my box and wiped down with rubbing alcohol, it has tyvec sleves and locking clamps so im pretty sure its a good SAB. after dumping, i placed the tray back inside of a plastic bag i had previously sprayed down with bleach and let evaporate, then tied the tray inside the bag and put it away. anyone think i gave myself contams?
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WolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
#22268717 - 09/21/15 10:30 AM (8 years, 4 months ago) |
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Quote:
kellogg said: i proceeded to load my tray and two more pints of uncolonized, sterilized wbs into my SAB, and dumped the wbs onto the surface of my tray, no breaking up of the tray or anything, just straight poured.
I don't understand why you put uncolonized wbs into a tray of colonized wbs. Probably not a good idea... I don't know what you are trying to accomplish. Are you following a specific tek?
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kellogg said: anyone think i gave myself contams?
You don't know if you have contamination until you have contamination. How can you ask people to speculate on that?
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kellogg
economist...ist


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Re: do you think its prone to contam? [Re: WolfieNuke]
#22268775 - 09/21/15 10:46 AM (8 years, 4 months ago) |
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1.for the same reason youd do grain to grain. to colonize.not following a tek. but am following sterile procedure to the best of my ability. i want bulk but cant afford a pressure cooker (yet) that holds any more than 4 pint jars.
2. im not the god of mushroom growing? others have more experience. im simply asking for peoples input who have tried/ experienced this.
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WolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
#22268825 - 09/21/15 10:55 AM (8 years, 4 months ago) |
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Quote:
kellogg said: 1.for the same reason youd do grain to grain. to colonize.not following a tek.
Grain to grain is one thing, but you spawned to coir already... When you spawn to bulk, you have now placed your spawn in an unclean environment. The only thing that prevents contamination is the fact that any nutrients are already colonized by your mushroom spawn and can resist contamination. By adding uncolonized grain, you have added a nutrient rich food in an unsterile environment. Not a good idea in my opinion.
You didn't do grain to grain. If you were to do that, you would not spawn your wbs to coir before doing grain to grain. You would put some of the colonized wbs in each sterilized jar of wbs.
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kellogg said: 2. im not the god of mushroom growing? others have more experience. im simply asking for peoples input who have tried/ experienced this
If you're not a mushroom growing god (I'm not one either), then why are you already not following a tried and true tek? Nobody can answer your question about contamination. They will ask the same questions and give you the same advice as I just did...
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Kizzle
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Re: do you think its prone to contam? [Re: WolfieNuke]
#22268838 - 09/21/15 10:58 AM (8 years, 4 months ago) |
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You took steps to keep it sterile but the problem is the tray was never truly sterile to begin with. I would remove as much of that uncolonized grain from the tray as you can ASAP.
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kellogg
economist...ist


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Re: do you think its prone to contam? [Re: Kizzle]
#22268850 - 09/21/15 11:00 AM (8 years, 4 months ago) |
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even with a bleached bag put around it and done inside of a sab it still would have a high chace of fighting contams?
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22268867 - 09/21/15 11:05 AM (8 years, 4 months ago) |
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also, the tray was basically 100% colonized. and you just said the only thing that prevents contam is the nutrients already being colonized and resisting contam. but, the tray is colonized. growth onto the uncolonized wbs will start within the next few days or even the next few hours, my myc already has a teather/ foothold in this
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22268878 - 09/21/15 11:07 AM (8 years, 4 months ago) |
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and tbh, since i spawned about 50/50 when i did this tray that means i have about 3-4 pints of colonized nutrients with more surface area to colonize. that was my original idea. more surface area with sterile conditions COULD = fastwr growth OR more contam resistance. I guess the only way we will know is if i keep you guys updated. thanks
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WolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
#22268883 - 09/21/15 11:08 AM (8 years, 4 months ago) |
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Quote:
kellogg said: even with a bleached bag put around it and done inside of a sab it still would have a high chace of fighting contams?
You ask for our opinions and then question them???
A still air box is not sterile. It's meant to limit the chance of other spores and bacteria from entering the work area by minimizing air flow. That's why any work done in a still air box is done quickly to minimize the time that any containers are open.
Bleach will sanitize, but it is not sterile. You have to realize that you are not in a clean room, and that whenever there is uncolonized grain (a food source) that is open to air, there is a good chance of contamination.
Do I think you have contamination??? I think you have a high chance, if I am understanding what you have done... Like I said, you won't know until you have it. Nobody else, can tell you any more than that.
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22268889 - 09/21/15 11:10 AM (8 years, 4 months ago) |
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and i keep posting without getting my point across. since i have the 3-4 pints of colonized material and i poured 2 pints in, im making a smaller leap than i did when spawning 50/50 wbs to coir, since now its about 75/25 , not really spawn.
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22268897 - 09/21/15 11:11 AM (8 years, 4 months ago) |
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isnt that how you learn? by asking questions? if you want to preach to people trying to learn, and limit your preaching when questions are asked, then you can go chose a different post theres thousands of different threads, thanks
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WolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
#22268901 - 09/21/15 11:12 AM (8 years, 4 months ago) |
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Quote:
kellogg said: and tbh, since i spawned about 50/50 when i did this tray that means i have about 3-4 pints of colonized nutrients with more surface area to colonize. that was my original idea. more surface area with sterile conditions COULD = fastwr growth OR more contam resistance. I guess the only way we will know is if i keep you guys updated. thanks
You are trying to re-invent the wheel, when you have little experience. My recommendation would be to stop thinking you know more than others that have come before you and follow a tek from a TC verbatim...
http://www.shroomery.org/forums/showflat.php/Number/16453495Quote:
kellogg said: and i keep posting without getting my point across. since i have the 3-4 pints of colonized material and i poured 2 pints in, im making a smaller leap than i did when spawning 50/50 wbs to coir, since now its about 75/25 , not really spawn.
Then if it's not spawn, why are you adding uncolonized grain? That's what I'm not understanding... or did I misinterpret you?
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kellogg
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Re: do you think its prone to contam? [Re: WolfieNuke]
#22268924 - 09/21/15 11:16 AM (8 years, 4 months ago) |
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re invent the wheel? lmao. wbs tek exist, i followed it. tray teks exist. i folloewd them. sab exists. i used it. uncolonized grain existed before i did.what am i inventing? if putting one material on top of another is inventing, then where did the material come from? i didnt invent the material. so how is it inventing again? its all proven methods, individually. one new factor isnt inventing. inventing is from absolute scratch. and its not spawn in the sence that i didnt mix it, but the point it its STILL touching an existing colony, my myc will get to it before a dust particle does. i asked for SPECULATION just like you stated.not certainty. speculation is not certainty. please get off of my post with your alpha apex hitler attitude, i was simply asking for speculation, not harassment
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WolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
#22268939 - 09/21/15 11:20 AM (8 years, 4 months ago) |
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kellogg said: re invent the wheel? lmao. wbs tek exist, i followed it. tray teks exist. i folloewd them. sab exists. i used it. uncolonized grain existed before i did.what am i inventing? if putting one material on top of another is inventing, then where did the material come from? i didnt invent the material. so how is it inventing again? its all proven methods, individually. one new factor isnt inventing. inventing is from absolute scratch.
"Re-inventing the wheel" is a metaphor. It means that you are trying to change something that has already been proven to be effective. I'm not calling you an inventor. LOL https://en.wikipedia.org/wiki/Reinventing_the_wheel
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kellogg said: isnt that how you learn? by asking questions? if you want to preach to people trying to learn, and limit your preaching when questions are asked, then you can go chose a different post theres thousands of different threads, thanks
You learn by listening to people, not just by asking questions and ignoring the responses.
I have told you that I do not think that your procedure is good, in terms of sterility - no matter how good you believe your sterile technique is. I don't think it is a good idea to add wbs to a bulk tray that is already colonized.
Don't flame me because you don't like my answer... I am trying to help. Good luck getting help from others acting like that. Many other people have appreciation and have been kind to me here, so I try to do the same in return.
I hope your grow goes well, and you don't have contamination...
Edited by WolfieNuke (09/21/15 11:29 AM)
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Kizzle
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Re: do you think its prone to contam? [Re: kellogg]
#22268959 - 09/21/15 11:24 AM (8 years, 4 months ago) |
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The difference is the uncolonized material this time is grain which needs to be kept completely sterile until fully colonized and is very vulnerable to contamination before that, whereas coir is not vulnerable. Which is why it's used as a bulk substrate and grain is not.
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kellogg
economist...ist


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Re: do you think its prone to contam? [Re: kellogg]
#22268963 - 09/21/15 11:24 AM (8 years, 4 months ago) |
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also when did i state that i knew more than anybody else? ". My recommendation would be to stop thinking you know more than others that have come before you" i CLEARLY stated that others have more exerience than me, and the fact that this post exists backs that up, so again what was your poing in putting wods in my mouth,, and acting hostile over your words you chose to make yourself believe came out of my mouth? youre clearly just trying to argue, go get a life, respectfully i have p[lenty of more hobbies than just this one, not that im baging on the hobby, its a fun one, but youve clearly degraded your life into a single forum considering the fact youre trying to harass others that are trying to learn xD please go harass some middle schoolers, theyll more likely be prone to your cancerous-conamitated like mentality... respectfully
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22268972 - 09/21/15 11:26 AM (8 years, 4 months ago) |
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the fact that you have more knowledge on the subject, gives you the right, in no form whatsoever, to harass others that know less. better yet put words n others mouths. good day
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iSmkGrnBud
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Re: do you think its prone to contam? [Re: kellogg]
#22268981 - 09/21/15 11:28 AM (8 years, 4 months ago) |
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Some people just can't be helped.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22268996 - 09/21/15 11:31 AM (8 years, 4 months ago) |
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advice with hostility is not advice, its being a total dick realize where the negativity in this post started before you point fingers
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22269001 - 09/21/15 11:32 AM (8 years, 4 months ago) |
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i mean for fucks sake, hes arguing with me for asking questions on my own post, then decided to put words in my mouth because of that? come on now, you can all grow up some if you think im the cause
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WolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
#22269005 - 09/21/15 11:33 AM (8 years, 4 months ago) |
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Quote:
kellogg said: advice with hostility is not advice, its being a total dick realize where the negativity in this post started before you point fingers
Please quote me where I harassed you... I've been more than civil, and everyone knows it... 
I'm done here...
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22269017 - 09/21/15 11:35 AM (8 years, 4 months ago) |
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even with a bleached bag put around it and done inside of a sab it still would have a high chace of fighting contams?
You ask for our opinions and then question them???
there you go, to spell out wether or not its worth crying over the ops attitude
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22269021 - 09/21/15 11:36 AM (8 years, 4 months ago) |
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how are you gona state the words" you ask for our opinions and question them??" and not sound like a fucking warlord who thinks everything he says is the one and only truth?
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iSmkGrnBud
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Re: do you think its prone to contam? [Re: kellogg]
#22269023 - 09/21/15 11:37 AM (8 years, 4 months ago) |
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Quote:
kellogg said: advice with hostility is not advice, its being a total dick realize where the negativity in this post started before you point fingers
http://www.shroomery.org/forums/showflat.php/Number/21448273
Stick to some of these teks. They're easy and fail-proof. Just follow step by step instructions. Once you get enough experience down you can then begin to experiment. Like everyone else stated before, you would never want to add un-colonized grain to already spawned sub, it's working backwards. It's absolutely more prone to contamination.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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kellogg
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Re: do you think its prone to contam? [Re: kellogg]
#22269029 - 09/21/15 11:38 AM (8 years, 4 months ago) |
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i never questioned anybodys opinions, as i have already rid my grow of the uncolonized wbs, you however, questioned my presence and my ability to learn freely
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kellogg
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Re: do you think its prone to contam? [Re: iSmkGrnBud]
#22269033 - 09/21/15 11:40 AM (8 years, 4 months ago) |
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Quote:
iSmkGrnBud said:
Quote:
kellogg said: advice with hostility is not advice, its being a total dick realize where the negativity in this post started before you point fingers
http://www.shroomery.org/forums/showflat.php/Number/21448273
Stick to some of these teks. They're easy and fail-proof. Just follow step by step instructions. Once you get enough experience down you can then begin to experiment. Like everyone else stated before, you would never want to add un-colonized grain to already spawned sub, it's working backwards. It's absolutely more prone to contamination. 
thanks for your responce, ive been following teks, this is the first time ive eperimented, simply due to the fustration with buying quart jars and not fitting into my pc. but i will look up on the rest of those teks in the link
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aerow.thefox
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Re: do you think its prone to contam? [Re: kellogg]
#22269307 - 09/21/15 12:59 PM (8 years, 4 months ago) |
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Quote:
kellogg said: also, the tray was basically 100% colonized. and you just said the only thing that prevents contam is the nutrients already being colonized and resisting contam. but, the tray is colonized. growth onto the uncolonized wbs will start within the next few days or even the next few hours, my myc already has a teather/ foothold in this
the reason we STERILIZE grain and never expose it to the elements until full colonization is that it is HIGHLY susceptible to contams. we PASTURIZE bulk subs and spawn to them in open air because pasturization leaves good bacteria that helps fight off contaminate spores frome germiinating before the mycelium, from THE 100% COLONIZED grain, fully colonizes it. by adding opening sterilized uncolonized grains and and spawning them to the sub, youve given a NONPASTURIZED substrate exposure to tons of contams. and theres no good bacteria to fight them off.
Edited by aerow.thefox (09/21/15 01:00 PM)
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kellogg
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Re: do you think its prone to contam? [Re: aerow.thefox]
#22271673 - 09/21/15 09:21 PM (8 years, 4 months ago) |
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thank you. so is it a thing, to just keep adding coir to an existing colony? also after a big tray is 100% colonized, could we break it up in a sterilized bag and redistribute to smaller trays? or does that still scream contam even if its 100% colonized and not being spawned, simply redistributed? stillanewbiguess
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cronicr



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Re: do you think its prone to contam? [Re: kellogg]
#22271687 - 09/21/15 09:23 PM (8 years, 4 months ago) |
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That is called super spawning and it sucks, just stick to the plan man!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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kellogg
economist...ist


Registered: 07/05/13
Posts: 49
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Re: do you think its prone to contam? [Re: cronicr]
#22271706 - 09/21/15 09:27 PM (8 years, 4 months ago) |
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Quote:
cronicr said: That is called super spawning and it sucks, just stick to the plan man!
okay thanks.wbs coir wbs coir wbs coir pizza pasta pesto.. allah khazam
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cronicr



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Re: do you think its prone to contam? [Re: kellogg]
#22271713 - 09/21/15 09:28 PM (8 years, 4 months ago) |
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bacon
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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