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Offlinekellogg
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do you think its prone to contam?
    #22268681 - 09/21/15 10:16 AM (8 years, 4 months ago)

okay so I've done a few grows but im still learning.
just jumped into bulk(ish) after 3 brf and 2 wbs successful grows.
i say "ish" because i decided to be careful, and only spawned about a pint and a half of wbs to some coir. idk like to hear peoples input on whether or not i screwed up. i just finished waiting about a week for my mini tray to colonize, it was basically 100% except two spots of coir less than a thumbtack small left uncolonized. i proceeded to load my tray and two more pints of uncolonized, sterilized wbs into my SAB, and dumped the wbs onto the surface of my tray, no breaking up of the tray or anything, just straight poured. i bleached the inside of my box and wiped down with rubbing alcohol, it has tyvec sleves and locking clamps so im pretty sure its a good SAB. after dumping, i placed the tray back inside of a plastic bag i had previously sprayed down with bleach and let evaporate, then tied the tray inside the bag and put it away. anyone think i gave myself contams?


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OfflineWolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
    #22268717 - 09/21/15 10:30 AM (8 years, 4 months ago)

Quote:

kellogg said:
i proceeded to load my tray and two more pints of uncolonized, sterilized wbs into my SAB, and dumped the wbs onto the surface of my tray, no breaking up of the tray or anything, just straight poured.



I don't understand why you put uncolonized wbs into a tray of colonized wbs. Probably not a good idea... I don't know what you are trying to accomplish. Are you following a specific tek?
Quote:

kellogg said:
anyone think i gave myself contams?



You don't know if you have contamination until you have contamination. How can you ask people to speculate on that?


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Offlinekellogg
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Re: do you think its prone to contam? [Re: WolfieNuke]
    #22268775 - 09/21/15 10:46 AM (8 years, 4 months ago)

1.for the same reason youd do grain to grain. to colonize.not following a tek. but am following sterile procedure to the best of my ability. i want bulk but cant afford a pressure cooker (yet) that holds any more than 4 pint jars.

2. im not the god of mushroom growing? others have more experience. im simply asking for peoples input who have tried/ experienced this.


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OfflineWolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
    #22268825 - 09/21/15 10:55 AM (8 years, 4 months ago)

Quote:

kellogg said:
1.for the same reason youd do grain to grain. to colonize.not following a tek.



Grain to grain is one thing, but you spawned to coir already... When you spawn to bulk, you have now placed your spawn in an unclean environment. The only thing that prevents contamination is the fact that any nutrients are already colonized by your mushroom spawn and can resist contamination. By adding uncolonized grain, you have added a nutrient rich food in an unsterile environment. Not a good idea in my opinion.

You didn't do grain to grain. If you were to do that, you would not spawn your wbs to coir before doing grain to grain. You would put some of the colonized wbs in each sterilized jar of wbs.
Quote:

kellogg said:
2. im not the god of mushroom growing? others have more experience. im simply asking for peoples input who have tried/ experienced this



If you're not a mushroom growing god (I'm not one either), then why are you already not following a tried and true tek? Nobody can answer your question about contamination. They will ask the same questions and give you the same advice as I just did...


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OfflineKizzle
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Re: do you think its prone to contam? [Re: WolfieNuke]
    #22268838 - 09/21/15 10:58 AM (8 years, 4 months ago)

You took steps to keep it sterile but the problem is the tray was never truly sterile to begin with. I would remove as much of that uncolonized grain from the tray as you can ASAP.


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Offlinekellogg
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Re: do you think its prone to contam? [Re: Kizzle]
    #22268850 - 09/21/15 11:00 AM (8 years, 4 months ago)

even with a bleached bag put around it and done inside of a sab it still would have a high chace of fighting contams?


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Offlinekellogg
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Re: do you think its prone to contam? [Re: kellogg]
    #22268867 - 09/21/15 11:05 AM (8 years, 4 months ago)

also, the tray was basically 100% colonized. and you just said the only thing that prevents contam is the nutrients already being colonized and resisting contam. but, the tray is colonized. growth onto the uncolonized wbs will start within the next few days or even the next few hours, my myc already has a teather/ foothold in this


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Offlinekellogg
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Re: do you think its prone to contam? [Re: kellogg]
    #22268878 - 09/21/15 11:07 AM (8 years, 4 months ago)

and tbh, since i spawned about 50/50 when i did this tray that means i have about 3-4 pints of colonized nutrients with more surface area to colonize. that was my original idea. more surface area with sterile conditions COULD = fastwr growth OR more contam resistance. I guess the only way we will know is if i keep you guys updated. thanks


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OfflineWolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
    #22268883 - 09/21/15 11:08 AM (8 years, 4 months ago)

Quote:

kellogg said:
even with a bleached bag put around it and done inside of a sab it still would have a high chace of fighting contams?



You ask for our opinions and then question them???

A still air box is not sterile. It's meant to limit the chance of other spores and bacteria from entering the work area by minimizing air flow. That's why any work done in a still air box is done quickly to minimize the time that any containers are open.

Bleach will sanitize, but it is not sterile. You have to realize that you are not in a clean room, and that whenever there is uncolonized grain (a food source) that is open to air, there is a good chance of contamination.

Do I think you have contamination??? I think you have a high chance, if I am understanding what you have done... Like I said, you won't know until you have it. Nobody else, can tell you any more than that.


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Offlinekellogg
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Re: do you think its prone to contam? [Re: kellogg]
    #22268889 - 09/21/15 11:10 AM (8 years, 4 months ago)

and i keep posting without getting my point across. since i have the 3-4 pints of colonized material and i poured 2 pints in, im making a smaller leap than i did when spawning 50/50 wbs to coir, since now its about 75/25 , not really spawn.


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Offlinekellogg
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Re: do you think its prone to contam? [Re: kellogg]
    #22268897 - 09/21/15 11:11 AM (8 years, 4 months ago)

isnt that how you learn? by asking questions? if you want to preach to people trying to learn, and limit your preaching when questions are asked, then you can go chose a different post :smile: theres thousands of different threads, thanks


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OfflineWolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
    #22268901 - 09/21/15 11:12 AM (8 years, 4 months ago)

Quote:

kellogg said:
and tbh, since i spawned about 50/50 when i did this tray that means i have about 3-4 pints of colonized nutrients with more surface area to colonize. that was my original idea. more surface area with sterile conditions COULD = fastwr growth OR more contam resistance. I guess the only way we will know is if i keep you guys updated. thanks



You are trying to re-invent the wheel, when you have little experience. My recommendation would be to stop thinking you know more than others that have come before you and follow a tek from a TC verbatim...

http://www.shroomery.org/forums/showflat.php/Number/16453495
Quote:

kellogg said:
and i keep posting without getting my point across. since i have the 3-4 pints of colonized material and i poured 2 pints in, im making a smaller leap than i did when spawning 50/50 wbs to coir, since now its about 75/25 , not really spawn.



Then if it's not spawn, why are you adding uncolonized grain? That's what I'm not understanding... or did I misinterpret you?


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Offlinekellogg
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Re: do you think its prone to contam? [Re: WolfieNuke]
    #22268924 - 09/21/15 11:16 AM (8 years, 4 months ago)

re invent the wheel? lmao. wbs tek exist, i followed it. tray teks exist. i folloewd them. sab exists. i used it. uncolonized grain existed before i did.what am i inventing? if putting one material on top of another is inventing, then where did the material come from?  i didnt invent the material.  so how is it inventing again? its all proven methods, individually. one new factor isnt inventing. inventing is from absolute scratch. and its not spawn in the sence that i didnt mix it, but the point it its STILL touching an existing colony, my myc will get to it before a dust particle does. i asked for SPECULATION just like you stated.not certainty. speculation is not certainty. please get off of my post with your alpha apex hitler attitude, i was simply asking for speculation, not harassment :smile:


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OfflineWolfieNuke
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Re: do you think its prone to contam? [Re: kellogg]
    #22268939 - 09/21/15 11:20 AM (8 years, 4 months ago)

kellogg said:
re invent the wheel? lmao. wbs tek exist, i followed it. tray teks exist. i folloewd them. sab exists. i used it. uncolonized grain existed before i did.what am i inventing? if putting one material on top of another is inventing, then where did the material come from?  i didnt invent the material.  so how is it inventing again? its all proven methods, individually. one new factor isnt inventing. inventing is from absolute scratch.



"Re-inventing the wheel" is a metaphor. It means that you are trying to change something that has already been proven to be effective. I'm not calling you an inventor. LOL
https://en.wikipedia.org/wiki/Reinventing_the_wheel

Quote:

kellogg said:
isnt that how you learn? by asking questions? if you want to preach to people trying to learn, and limit your preaching when questions are asked, then you can go chose a different post :smile: theres thousands of different threads, thanks



You learn by listening to people, not just by asking questions and ignoring the responses.

I have told you that I do not think that your procedure is good, in terms of sterility - no matter how good you believe your sterile technique is. I don't think it is a good idea to add wbs to a bulk tray that is already colonized.

Don't flame me because you don't like my answer... I am trying to help. Good luck getting help from others acting like that. Many other people have appreciation and have been kind to me here, so I try to do the same in return.

I hope your grow goes well, and you don't have contamination...


Edited by WolfieNuke (09/21/15 11:29 AM)


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OfflineKizzle
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Re: do you think its prone to contam? [Re: kellogg]
    #22268959 - 09/21/15 11:24 AM (8 years, 4 months ago)

The difference is the uncolonized material this time is grain which needs to be kept completely sterile until fully colonized and is very vulnerable to contamination before that, whereas coir is not vulnerable. Which is why it's used as a bulk substrate and grain is not.


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Offlinekellogg
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Re: do you think its prone to contam? [Re: kellogg]
    #22268963 - 09/21/15 11:24 AM (8 years, 4 months ago)

also when did i state that i knew more than anybody else? ". My recommendation would be to stop thinking you know more than others that have come before you"  i CLEARLY stated that others have more exerience than me, and the fact that this post exists backs that up, so again what was your poing in putting wods in my mouth,, and acting hostile over your words you chose to make yourself believe came out of my mouth? youre clearly just trying to argue, go get a life, respectfully :smile: i have p[lenty of more hobbies than just this one, not that im baging on the hobby, its a fun one, but youve clearly degraded your life into a single forum considering the fact youre trying to harass others that are trying to learn xD please go harass some middle schoolers, theyll more likely be prone to your cancerous-conamitated like mentality... respectfully :smile:


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Offlinekellogg
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Re: do you think its prone to contam? [Re: kellogg]
    #22268972 - 09/21/15 11:26 AM (8 years, 4 months ago)

the fact that you have more knowledge on the subject, gives you the right, in no form whatsoever, to harass others that know less. better yet put words n others mouths. good day :laugh:


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OfflineiSmkGrnBud
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Re: do you think its prone to contam? [Re: kellogg]
    #22268981 - 09/21/15 11:28 AM (8 years, 4 months ago)

:smbfacepalm: Some people just can't be helped.


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Offlinekellogg
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Re: do you think its prone to contam? [Re: kellogg]
    #22268996 - 09/21/15 11:31 AM (8 years, 4 months ago)

advice with hostility is not advice, its being a total dick :smile: realize where the negativity in this post started before you point fingers


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Offlinekellogg
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Re: do you think its prone to contam? [Re: kellogg]
    #22269001 - 09/21/15 11:32 AM (8 years, 4 months ago)

i mean for fucks sake,  hes arguing with me for asking questions on my own post, then decided to put words in my mouth because of that? come on now, you can all grow up some if you think im the cause


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