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OfflineDrmantus
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Can Spore grow in Syringe
    #22267535 - 09/20/15 11:09 PM (8 years, 4 months ago)

So i have this syringe in my closet for over 5 years now, and inside was what looked like Mycelium growing and not seeds. Is this possible?

So i inoculated a jar 5 days later i am seeing first growth of what looks like some healthy Mycelium.
I read somewhere that you can put jar in the fridge and remove to induce faster growth is this true?


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OfflineYaMoonSun
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22267541 - 09/20/15 11:12 PM (8 years, 4 months ago)

Sounds odd, but good luck. Update thread with pictures later.
The spores need nutrients to grow, or so i thought, but maybe they can stress out over long periods of time.


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OfflineAchillita
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22267543 - 09/20/15 11:14 PM (8 years, 4 months ago)

5 years is a long time for a spore syringe. Although I'd imagine it's possible for mycelium to grow in a syringe, it'd be pretty unlikely to grow, and have no FAE.:shrug:

Well, pics please. Also don't put it in the fridge, it won't cause it to grow any faster. Room temperature is good.


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Invisiblewowimflabbergasted
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Re: Can Spore grow in Syringe [Re: Achillita]
    #22267662 - 09/20/15 11:51 PM (8 years, 4 months ago)

Spores can germinate and grow inside of the syringe, yes. It happens less if you is distilled water to make syringes since there are no nutes to feed on.


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OfflineAchillita
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Re: Can Spore grow in Syringe [Re: wowimflabbergasted]
    #22267673 - 09/20/15 11:53 PM (8 years, 4 months ago)

But last for 5 years?! That's so crazy if it works out.


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Invisiblewowimflabbergasted
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Re: Can Spore grow in Syringe [Re: Achillita]
    #22267680 - 09/20/15 11:57 PM (8 years, 4 months ago)

Yeah I've used vendor syringes for up to 3 or so years now and been fine. All just kept in my closet lol probably not the best spot but oh well :shrug:


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Invisibletahoe
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Re: Can Spore grow in Syringe [Re: wowimflabbergasted]
    #22267747 - 09/21/15 12:18 AM (8 years, 4 months ago)

5 Years is nothing for a spore syringe. They could probably last a lot longer. I am currently using 5 year old syringes.



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Offlinefear-and-loathing
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Re: Can Spore grow in Syringe [Re: wowimflabbergasted]
    #22267754 - 09/21/15 12:20 AM (8 years, 4 months ago)

i have some 3 yo syringes and prints i hope they work


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OfflineAchillita
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Re: Can Spore grow in Syringe [Re: fear-and-loathing]
    #22267773 - 09/21/15 12:27 AM (8 years, 4 months ago)

:shrug: I've seen repeated all over this site about syringes not lasting more than 6 months to a year, and prints only lasting a couple of years.

This information needs to be spread around a bit more imo.


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OfflineMiNdTwIsTeR
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Re: Can Spore grow in Syringe [Re: Achillita]
    #22267800 - 09/21/15 12:42 AM (8 years, 4 months ago)

Quote:

Achillita said:
:shrug: I've seen repeated all over this site about syringes not lasting more than 6 months to a year, and prints only lasting a couple of years.

This information needs to be spread around a bit more imo.




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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: MiNdTwIsTeR]
    #22267815 - 09/21/15 12:52 AM (8 years, 4 months ago)

Well i will post pics when i can.
Unfortunately did not take any pics before emptying syringe.
It was white floating around like a liquid culture.

Strange but wish me luck the strain i think is Shooting Stars any one worked with these?


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22272349 - 09/22/15 01:10 AM (8 years, 4 months ago)



So far looks health to me.
Kind of a large jar and only had a small amount in the syringe.


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Invisiblegizmo1
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22272358 - 09/22/15 01:19 AM (8 years, 4 months ago)

WTH is in that jar looks like manure straw and brown rice lol?


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OfflineAchillita
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Re: Can Spore grow in Syringe [Re: gizmo1]
    #22272539 - 09/22/15 03:38 AM (8 years, 4 months ago)

What's in the jar?


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: Achillita]
    #22272627 - 09/22/15 04:40 AM (8 years, 4 months ago)

Lol with a short amount of supplies I whipped something together.
Ingredients are:
Coco nut coir which looks like straw
Brown rice flour
Some red wood bark for moisture
And some coffee grounds
It's a crazy mix but I am giving it a shot
So wish me luck.


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22272629 - 09/22/15 04:41 AM (8 years, 4 months ago)

Would you say that looks alright for a 5 yr old syringe.


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InvisibleDaveyJones6911
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22272723 - 09/22/15 05:48 AM (8 years, 4 months ago)

it's mycelium.
for a 5yo syringe i would say it looks awesome :laugh:
gives me some hope for my 10yo+ prints :P


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: DaveyJones6911]
    #22597722 - 12/01/15 03:35 AM (8 years, 1 month ago)

Update:
Check on my jar a couple days ago seemed to come to a hault, I checked today and it has grown a lot in 2 days.

There is a little yellowing around the top of the jar and in the middle it is small it concerns me but I believe it is only my substrate.

So far she is still looking healthy.
Any comments would be appreciated.


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22597728 - 12/01/15 03:39 AM (8 years, 1 month ago)

I am moving this jar ^ into my garage for a night thinking maybe it will give the jar a little coldness, so when returning it the growth rate will increase.

Does this work it seems like it would in theory. At night it gets cold and mushrooms do grow outside.


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Offlinecontamanoid
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22597759 - 12/01/15 04:11 AM (8 years, 1 month ago)

yes


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OfflineFreeWorldOrder
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Re: Can Spore grow in Syringe [Re: contamanoid]
    #22598126 - 12/01/15 08:32 AM (8 years, 1 month ago)

lol... you want room temps for colonization. 70-75 works great. There should be no reason to cold shock a colonizing substrate. It will only slow it down. There are some varieties of mushrooms that benefit a cold shock to induce pinning but any of the cubes I have experience with do not.

Also, what are using for a filter for gas exchange?

I just wondered because we usually remove the foil when we inoculate then leave it off. I assume you are using some type of filter or filter material right?


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OfflineJabensis
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Re: Can Spore grow in Syringe [Re: FreeWorldOrder]
    #22598266 - 12/01/15 09:13 AM (8 years, 1 month ago)

Ive had spore solution (that I prepared myself) grow myc.... and it took to grain surprisingly.

I would deposit a few drops of this liquid onto a few agar plates and hope it grows.

I certainly would not waste time injecting into grain.

J


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Offlinekyu
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Re: Can Spore grow in Syringe [Re: Jabensis]
    #22598969 - 12/01/15 12:12 PM (8 years, 1 month ago)

it needed 2 months to reach 50% of the jar?
i'm pessimistic about the future yield (if there will be any)


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: kyu]
    #22599265 - 12/01/15 01:40 PM (8 years, 1 month ago)

Quote:

kyu said:
it needed 2 months to reach 50% of the jar?
i'm pessimistic about the future yield (if there will be any)




Indeed a long time but it is also a huge jar. I plan to break some of it into a larger casing.


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InvisibleSupalemonhaze
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22599294 - 12/01/15 01:48 PM (8 years, 1 month ago)

Quote:

Drmantus said:
I am moving this jar ^ into my garage for a night thinking maybe it will give the jar a little coldness, so when returning it the growth rate will increase.

Does this work it seems like it would in theory. At night it gets cold and mushrooms do grow outside.




Cold shocking has been debunked as far as I know. Reading threads of people who store mycelium in fridges, they say that they usually give them a day or two for them to recover. I think cold shocking will slow it down rather than speed it up. IMO you should shake it if you want it to colonise faster.


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22601143 - 12/01/15 08:04 PM (8 years, 1 month ago)

Quote:

Drmantus said:


So far she is still looking healthy.

Is this jar looking alright? It hopefully shouldn't be any longer.





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OfflineYaMoonSun
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22601875 - 12/01/15 10:13 PM (8 years, 1 month ago)

Going to fruit it invitro?


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: YaMoonSun]
    #22602622 - 12/02/15 01:23 AM (8 years, 1 month ago)

Quote:

YaMoonSun said:
Going to fruit it invitro?




It is possible but is not my intention.

How do you prevent this by keeping in darkness.

I want to continue some of this myc into a spawn bag


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OfflineDrmantus
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22611681 - 12/04/15 04:56 AM (8 years, 1 month ago)

So this is were I am at the photos below are what it still looks like. Growth has slowed down.

What I want to do is cut this cake up into a casing, do I dunk a hydrate before braking it up.

Would you say this jar looks healthy enough to remove, at this rate I want to avoid early pinning.









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InvisibleSupalemonhaze
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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22612592 - 12/04/15 11:35 AM (8 years, 1 month ago)

If it is stalling my guess would be that something else is growing in that jar. I wouldn't put mycelium from a stalled jar in a spawnbag if you are still thinking about that.

Anyways yeah, you should dunk it before crumbling. Especially if you are not gonna add bulk(which I wouldn't since you probably have something else growing in there). Bulk gives moisture more than it gives nutes and we still hydrate grains before spawning. I never crumbled cakes before but I think that is the way to go.


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OfflineMandarinfish

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Re: Can Spore grow in Syringe [Re: Drmantus]
    #22612647 - 12/04/15 11:56 AM (8 years, 1 month ago)

.


Edited by Mandarinfish (07/17/20 08:14 PM)


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Re: Can Spore grow in Syringe [Re: Mandarinfish]
    #22612659 - 12/04/15 11:59 AM (8 years, 1 month ago)

jars were too big and sub was too wet


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OfflineMandarinfish

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Re: Can Spore grow in Syringe [Re: cronicr]
    #22612793 - 12/04/15 12:46 PM (8 years, 1 month ago)

.


Edited by Mandarinfish (07/17/20 08:15 PM)


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