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XtheXblackXsheepX
Stranger
Registered: 09/20/15
Posts: 13
Last seen: 6 years, 8 months
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First PF Tek - Possible Botched Attempt - Pics
#22263904 - 09/20/15 09:16 AM (8 years, 4 months ago) |
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Hello all,
I recently acquired two spore syringes (b+ and PE) and wanted to give the PF Tek a shot. I inoculated my jars today and think that I made a vital error in the procedure. In the 2nd video in the RR PF Tek series, the man inoculates his jars by piercing the tape with the needle rather than taking the tape off, sticking the needle through the hole, injecting, and then replacing the tape. When I did this, the hot needle against the tape produced a black soot type substance at the inoculation site.
I used a 10cc B+ spore syringe for 4 1/2 pint jars. I steam sterilized the jars for 100 minutes last night and let the jars cool until this morning. I wore gloves during the procedure, sterilized my glove box, sterilized my gloves, flame sterilized the needle between each jar w/ a tea-light candle.
I'm assuming that I'll just have to take a loss on these jars but any advice is appreciated!
TBS
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jbaby007
Badass



Registered: 02/28/15
Posts: 1,026
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I inoculated my pf jars in open air without piercing the needle through tape. The tape is unnecessary since you have a dry verm layer.
The black soot is most likely from you flaming your needle with a lighter instead of an alcohol flame lamp which is perfectly fine. That soot won't hurt anything. Some people just don't like the look of it so they use alcohol lamps. Good luck! Sounds like you're on the right track dude. 
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DaveyJones6911
Nonconformist


Registered: 09/08/15
Posts: 690
Loc: EU
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i'm not reading anything that suggests a problem to me.
soot is sterile, and a tiny bit of burnt glue isn't going to hurt anything. i just did some plastic jars that didn't have holes yet so i just jammed the still hot needle through the plastic to make them, then covered them with tape. all jars have germinated and are doing just fine. and i didn't even wear gloves.
it's probably fine. keep the jars and wait to see what happens.
-------------------- Creativity is a dangerous thing in the eyes of those who don't have any.
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XtheXblackXsheepX
Stranger
Registered: 09/20/15
Posts: 13
Last seen: 6 years, 8 months
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Re: First PF Tek - Possible Botched Attempt - Pics [Re: DaveyJones6911]
#22264077 - 09/20/15 10:19 AM (8 years, 4 months ago) |
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Thank you both so much for your responses!
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: First PF Tek - Possible Botched Attempt - Pics [Re: DaveyJones6911]
#22264080 - 09/20/15 10:20 AM (8 years, 4 months ago) |
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How much did you use per pint jar? 10cc for 4 1/2 pint jars is a lot.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First PF Tek - Possible Botched Attempt - Pics [Re: taGyo]
#22264141 - 09/20/15 10:33 AM (8 years, 4 months ago) |
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Indeed a 10cc syringe should do bare minimum 10 jars but more.like 20 if you're good
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XtheXblackXsheepX
Stranger
Registered: 09/20/15
Posts: 13
Last seen: 6 years, 8 months
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Re: First PF Tek - Possible Botched Attempt - Pics [Re: bodhisatta]
#22264892 - 09/20/15 01:17 PM (8 years, 4 months ago) |
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Yes, I used all 10cc's for 4 1/2 pint jars. I read that I was able to do 10 jars with 10cc's, but 10 seemed excessive for my first time... After seeing where I go wrong w/ this trial run over the next few weeks, I will more than likely take this knowledge and use my PE syringe on 10 jars versus 4.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Brotha.. You need 1cc per jar... Possibly .25-.5 once you get the hang of it. You used A LOT per jar.
You could have messed up your moisture content, BRF is a little sensitive to this. Time will tell. Just wait it out.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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jbaby007
Badass



Registered: 02/28/15
Posts: 1,026
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Re: First PF Tek - Possible Botched Attempt - Pics [Re: taGyo]
#22265366 - 09/20/15 02:53 PM (8 years, 4 months ago) |
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When I read that, I didn't think he used the whole syringe for 4 jars lol.
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