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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,036
Loc: Themyscira Flag
Bad Syringe or Bad Technique?
    #22263709 - 09/20/15 07:47 AM (8 years, 4 months ago)

Last week, I made up some BRF cakes and steamed them for 90 minutes as usual.  I inoculated the jars the next day with a Lizard King syringe.  After a week, there's no growth in any of the jars.  They all smell like sweaty feet dipped in cake frosting...bad.  You don't even have to put your nose to the jar to smell it, just open the closet door and it smacks you in the face.

How do you know if you got a bad syringe vs your sterilization technique?  I got the syringe from a Sponsor.  I'd hate to go back to them and ask for a new syringe if its something that I did.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Bad Syringe or Bad Technique? [Re: ShroomerInTheRye]
    #22264101 - 09/20/15 10:25 AM (8 years, 4 months ago)

The MS syringe is most likely the cause of the contam, pressure cooking for 90 minutes at 15 psi is more than enough time to thoroughly sterilize the jars.

I would suggest working with agar and making an LI syringe to knock up your jars. That way you'll know that you have clean growth and colonization times will be greatly increased  :thumbup:


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InvisibleMad Season
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Registered: 09/16/12
Posts: 12,666
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Re: Bad Syringe or Bad Technique? [Re: Psilosoulful]
    #22273795 - 09/22/15 12:20 PM (8 years, 4 months ago)

90 minutes at 15 (250F) is not enough at all to sterilize jars with high endospore count.

I do 20-22 psi (260F) for 120 minutes, and still see bacteria here and there.

In my signature it says what Paul Stamets figured out for bacterial endospores. Imo 27 at 90 minutes would be more effective.


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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag


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