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Offlinememberjockey
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pan cyan agar clone LC G2G transfer
    #22261766 - 09/19/15 07:43 PM (8 years, 4 months ago)

A very good evening to everybody!

I'm working on an epic pan cyan grow, I started with inoculating agar plates with a print of Tasmania. Then I transferred them to new plates and inoculated some grain jars
and spawned some of the jars to bulk & cased then put into the fruiting chamber.. One of the first shroom to fruit was a fattie and became my first clone. After a couple of transfers some are ready to use.The jars were inoculated with the clone dishes 2 days ago, I'll shake them tonight. a few days ago I also transferred wbs from a master jar to 3 new jars of rye. and for giggles I dropped a strand into a liquid culture and so far so good. A tiny cake. 3.5 days into the spawn run.My outdoor tub

I hope you enjoyed my pictures I'll keep you updated as it all progresses.


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Offlinepsylo-not
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22262020 - 09/19/15 08:44 PM (8 years, 4 months ago)

Absolutely gorgeous! :rockon:


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OfflinetaGyo
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Re: pan cyan agar clone LC G2G transfer [Re: psylo-not]
    #22262045 - 09/19/15 08:49 PM (8 years, 4 months ago)

:pipesmoke:


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InvisibleFantastic Mr. Fox
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22262080 - 09/19/15 08:56 PM (8 years, 4 months ago)

:popcorn:


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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22262094 - 09/19/15 08:59 PM (8 years, 4 months ago)

Nice to see ya mj! been awhile, glad to see ya still messing with pans


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OfflineiSmkGrnBud
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Re: pan cyan agar clone LC G2G transfer [Re: cronicr]
    #22262171 - 09/19/15 09:12 PM (8 years, 4 months ago)

Nice! :cheers:


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Edited by iSmkGrnBud (09/19/15 09:22 PM)


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InvisibleFantastic Mr. Fox
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Re: pan cyan agar clone LC G2G transfer [Re: iSmkGrnBud]
    #22262203 - 09/19/15 09:18 PM (8 years, 4 months ago)

She's a girl/woman..


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OfflineiSmkGrnBud
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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22262243 - 09/19/15 09:24 PM (8 years, 4 months ago)

Quote:

Fantastic Mr. Fox said:
She's a girl/woman..



Good call, my mistake :thumbup:


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Invisibleazur
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22262291 - 09/19/15 09:34 PM (8 years, 4 months ago)

Quote:

memberjockey said:
A very good evening to everybody!

I'm working on an epic pan cyan grow, I started with inoculating agar plates with a print of Tasmania. Then I transferred them to new plates and inoculated some grain jars
and spawned some of the jars to bulk & cased then put into the fruiting chamber.. One of the first shroom to fruit was a fattie and became my first clone. After a couple of transfers some are ready to use.The jars were inoculated with the clone dishes 2 days ago, I'll shake them tonight. a few days ago I also transferred wbs from a master jar to 3 new jars of rye. and for giggles I dropped a strand into a liquid culture and so far so good. A tiny cake. 3.5 days into the spawn run.My outdoor tub

I hope you enjoyed my pictures I'll keep you updated as it all progresses.





--------------------


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FOTTSE!!!
ALL NOOBS READ THIS!!!



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Invisibleelasticaltiger
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Re: pan cyan agar clone LC G2G transfer [Re: azur]
    #22262407 - 09/19/15 09:59 PM (8 years, 4 months ago)

This is badass.

:nodofunderstanding:


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22262592 - 09/19/15 10:33 PM (8 years, 4 months ago)

How great is this?!

What sort of substrate do you use for the bulk tubs?


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Offlinememberjockey
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Re: pan cyan agar clone LC G2G transfer [Re: champinhom]
    #22266341 - 09/20/15 06:30 PM (8 years, 4 months ago)

I always use chopped straw, manure and gypsum for both cube and pans.

Here are some more pictures of colonized jars of pan that you can really see the beautiful growth patterns. I think using manure in my agar may be one of the reasons the jars look so nice. These are half gallon jars not quart.


Some of my manure agar plates.



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InvisibleLocN9ne
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22266350 - 09/20/15 06:32 PM (8 years, 4 months ago)

Bro... is that green all over in that shot from the top?


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22266393 - 09/20/15 06:38 PM (8 years, 4 months ago)

Manure agar?  Expound please


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Offlinememberjockey
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Re: pan cyan agar clone LC G2G transfer [Re: LocN9ne]
    #22266409 - 09/20/15 06:43 PM (8 years, 4 months ago)

Here's a better pic from the top.



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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22266663 - 09/20/15 07:42 PM (8 years, 4 months ago)

I soak composted manure in water strain out any sticks but leave some of the silt add your favorite agar mix to it and stir until there's no lumps then heat it slowly until it's starts to get hot then keep the silt stirred before you pour.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22266678 - 09/20/15 07:47 PM (8 years, 4 months ago)

Nice mj!


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I'm tired do me a favor


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OfflineMMG
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Re: pan cyan agar clone LC G2G transfer [Re: cronicr]
    #22338366 - 10/05/15 08:01 PM (8 years, 3 months ago)

Nice pics! the growth on those jars looks very nice, better than mine at least haha
I'm gonna try the hpoo agar, about how much hpoo do you put in for every 500ml water?


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:heart:
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Edited by MMG (10/05/15 11:19 PM)


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InvisibleFantastic Mr. Fox
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Re: pan cyan agar clone LC G2G transfer [Re: MMG]
    #22338372 - 10/05/15 08:03 PM (8 years, 3 months ago)

Any updates, jockey?


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Offlinememberjockey
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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22744109 - 01/06/16 01:17 PM (8 years, 25 days ago)

I hope everybody had a merry christmas and a happy new year, without going into any details I'm going to pick up like I wasn't gone for a few months if that's okay.

The first week of December I started new jars with agar made from my cloned mushroom which took about 3.5 weeks to colonize.   .

On the 29th of december I lined my first big tub with half a black trash bag and dusted the bottom with a little substrate broke up a half gallon jar of spawn evenly over the bottom, covered it with substrate, misted it then cover it leaving a small hole in the bottom where I can watch it grow.


The first tub colonized in 4 days I cased it a couple of days ago which was about 6 days into the spawn run.


Yesterday the other 4 large tubs were ready to be cased which makes 5 large tubs total.




The smaller tubs are lined with a white trash bag cut in half and 3 of those are colonized and cased bringing the total to 8 tubs that are ready to fruit.

.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey] * 1
    #22744130 - 01/06/16 01:22 PM (8 years, 25 days ago)

:crondance: nice to see ya mj!


--------------------

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I'm tired do me a favor


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Re: pan cyan agar clone LC G2G transfer [Re: cronicr] * 1
    #22744145 - 01/06/16 01:25 PM (8 years, 25 days ago)

I have a bunch of dishes that fruited but I just took this pic this morning.



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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22744156 - 01/06/16 01:28 PM (8 years, 25 days ago)

Thanks you have no idea how nice it is to be back.:aweyeah:


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22744179 - 01/06/16 01:35 PM (8 years, 25 days ago)

haha sweet! my pan cincs fruited on plates nbut none of my others have


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: pan cyan agar clone LC G2G transfer [Re: cronicr]
    #22744196 - 01/06/16 01:41 PM (8 years, 25 days ago)

I have psilocybe galindoi atlantis fruit on plates a lot.



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Offlinespacechildo
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22744231 - 01/06/16 01:48 PM (8 years, 25 days ago)

whoa mj, that's a blast from the past! :cheers:
I thought you got booted a long time ago, cool to see you're still playing around :wink:


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InvisibleFantastic Mr. Fox
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22744907 - 01/06/16 04:43 PM (8 years, 25 days ago)

:popcorn::popcorn:


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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22745008 - 01/06/16 05:14 PM (8 years, 24 days ago)

I just popped this cake out of one of the smallest tubs, I'm going to case it and stick it into another tub to fruit.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22745936 - 01/06/16 08:44 PM (8 years, 24 days ago)

That has to be the whitest sub I've ever seen


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OfflinetaGyo
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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22746157 - 01/06/16 09:35 PM (8 years, 24 days ago)

Wow it looks like vanilla frosting lol.


--------------------
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22746672 - 01/07/16 12:04 AM (8 years, 24 days ago)

Quote:

memberjockey said:




:mindblown:
What is the sub composed of and how'd you prep it?


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Offlinememberjockey
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Re: pan cyan agar clone LC G2G transfer [Re: Psilosoulful]
    #22747257 - 01/07/16 07:25 AM (8 years, 24 days ago)

Good morning everybody, this morning a few of the tubs have gone thru the casing, I've patched a couple of them and then misted them down again. Were they not getting enough fae?


I didn't get a chance to case the smallest cake last night I covered him with the bottom part of his tub.


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InvisibleFantastic Mr. Fox
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22747284 - 01/07/16 07:33 AM (8 years, 24 days ago)

G'morning.

Don't patch them, leave them be & let it rock.
You want your casing 30-40% colonized anyway.
&& stop misting them.


--------------------
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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22747503 - 01/07/16 09:02 AM (8 years, 24 days ago)

Okay no more patching, I won't patch any of the other ones.


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InvisibleAdden
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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22747670 - 01/07/16 09:50 AM (8 years, 24 days ago)

Toggled thread as favorite.

Thanks for sharing your experiment :wink:


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Offlineukshroomer
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Re: pan cyan agar clone LC G2G transfer [Re: Adden]
    #22748133 - 01/07/16 12:06 PM (8 years, 24 days ago)

keep us updated:mushroom2:


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Re: pan cyan agar clone LC G2G transfer [Re: Psilosoulful]
    #22748995 - 01/07/16 03:58 PM (8 years, 24 days ago)

I made a huge compost from straw, manure, vermiculite, gypsum and water that I've tended over the course of a couple of years in giant plastic tubs and let it dry out.


I added equal amounts of vermiculite to the mix plus a pinch more gypsum mixed it all up really good and took out about a quarter of it and put it aside. I have a bucket of black cow chunks sitting outside that collects rain water and I stir it up sift it out and wet down the mix until it's drippy then I mix in the rest of the dry ingredients to get it to field capacity then I put into cooking bags & pop it into a giant tortilla warmer and heat it to 140-145 for one hour. I use manure water for all my substrates.


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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22749042 - 01/07/16 04:06 PM (8 years, 24 days ago)

Quote:

Fantastic Mr. Fox said:
G'morning.

Don't patch them, leave them be & let it rock.
You want your casing 30-40% colonized anyway.
&& stop misting them.



i dont colonize my casing layers at all, i apply and fruit.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: pan cyan agar clone LC G2G transfer [Re: cronicr]
    #22749445 - 01/07/16 05:49 PM (8 years, 23 days ago)

They're in the fruiting chamber but they still are going thru the casing, I didn't patch these but I've got an itchy finger to patch them.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22749454 - 01/07/16 05:51 PM (8 years, 23 days ago)

They'll stop colonizing once pinning has begun.

DON'T PATCH.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22749476 - 01/07/16 05:57 PM (8 years, 23 days ago)

Here's a small and medium cake that I cased this evening.




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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22749512 - 01/07/16 06:06 PM (8 years, 23 days ago)

I made four light malt extract liquid cultures by cutting wedges of agar into it, they all have stir bars and get a rotation each day.



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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22749667 - 01/07/16 06:53 PM (8 years, 23 days ago)

This one's ready.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22749682 - 01/07/16 06:58 PM (8 years, 23 days ago)

Looks good, glad you're back as well :thumbup:


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Re: pan cyan agar clone LC G2G transfer [Re: Pastywhyte]
    #22749742 - 01/07/16 07:16 PM (8 years, 23 days ago)

Thanks Pasty it's very nice to be back and to contribute to the site, here are most of the cakes before being retired for the evening, I didn't patch these.



Two more of the large tubs and one of the medium one's


The one I case this morning and one more of the medium tubs.


This is the size of the 5 large ones.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22749826 - 01/07/16 07:40 PM (8 years, 23 days ago)

Some fruiting dishes of my cloned Tasmanian pan mushroom.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22751401 - 01/08/16 06:15 AM (8 years, 23 days ago)

Good morning shroomers, my tubs are still colonizing up the casing, they look like they are not getting enough fae or something, you can see the one's that haven't been patched are almost white again. Any suggestions? One of the tubs I patched yesterday has gone through the casing again the other not so much.



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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22751589 - 01/08/16 08:40 AM (8 years, 23 days ago)

Cant wait to see what kibd of flushes come from your incredibly healthy looking subs!


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Re: pan cyan agar clone LC G2G transfer [Re: Myco Dude]
    #22752911 - 01/08/16 02:27 PM (8 years, 23 days ago)

Another one of my large tubs was ready to case this morning along with the small and medium one that was ready yesterday.


This is one of the tester jars made from the liquid culture, I shook it last night for second time since it was inoculated 2 weeks ago, you can't see that it wasn't completely colonized on the back side before I shook it .



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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22753011 - 01/08/16 02:50 PM (8 years, 23 days ago)

A few of my beautiful fruiting dishes of my cloned pans.



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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22753130 - 01/08/16 03:26 PM (8 years, 23 days ago)

Quote:

memberjockey said:




Why are you stuffing the bottom holes with tyvek? :confused:
I've never seen that before, and not sure if that would keep the humidity levels high enough.
But hey, if it works, then I may just have to give it a try myself.

:themoreyouknow:


Edited by Psilosoulful (01/08/16 03:28 PM)


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Re: pan cyan agar clone LC G2G transfer [Re: Psilosoulful]
    #22764947 - 01/11/16 06:33 AM (8 years, 20 days ago)

My tubs keep colonizing the casing without fruiting, I patched the casing but it keeps doing this, I don't see any pins, does anybody with experience with this have any suggestions.



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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22764954 - 01/11/16 06:36 AM (8 years, 20 days ago)

Quote:

memberjockey said:
My tubs keep colonizing the casing without fruiting, I patched the casing but it keeps doing this, I don't see any pins, does anybody with experience with this have any suggestions.





Stop patching it. It doesn't understand and wants more fresh air at the surface IMO.

Wait for someone like Fantastic Fox though.


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Re: pan cyan agar clone LC G2G transfer [Re: taGyo]
    #22765034 - 01/11/16 07:29 AM (8 years, 20 days ago)

I have a pin, a side pin but a pin maybe it's a sign of good things to come.



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Re: pan cyan agar clone LC G2G transfer [Re: taGyo]
    #22765036 - 01/11/16 07:32 AM (8 years, 20 days ago)

I will not patch my casing again unless specified by a higher power.:facepalm3:


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22765174 - 01/11/16 08:30 AM (8 years, 20 days ago)

:lol:


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Re: pan cyan agar clone LC G2G transfer [Re: Psilosoulful]
    #22771536 - 01/12/16 06:42 PM (8 years, 18 days ago)

A few pins & a couple of mushrooms, yeah.



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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22771593 - 01/12/16 06:54 PM (8 years, 18 days ago)

Quote:

memberjockey said:
I will not patch my casing again unless specified by a higher power.:facepalm3:




lolol I told ya' not to patch them. :smirk:


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22771605 - 01/12/16 06:57 PM (8 years, 18 days ago)

I'd mist that casing layer a bit more often, the sgfc/hybrid tub gives tons of fae but also dries up your sub surface a bit quick, and pans love a good moist surface IME :wink:


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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22771658 - 01/12/16 07:04 PM (8 years, 18 days ago)

Quote:

Fantastic Mr. Fox said:
Quote:

memberjockey said:
I will not patch my casing again unless specified by a higher power.:facepalm3:




lolol I told ya' not to patch them. :smirk:



:rolleyes: they looked cold.


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Re: pan cyan agar clone LC G2G transfer [Re: spacechildo]
    #22771664 - 01/12/16 07:06 PM (8 years, 18 days ago)

I was told to quit misting or I think I was, I don't want to mist now since there are a few pins lurking about.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22771675 - 01/12/16 07:08 PM (8 years, 18 days ago)

Quote:

memberjockey said:
I was told to quit misting or I think I was, I don't want to mist now since there are a few pins lurking about.




aim for their feet if you're worried "dry caps wet feet",
but in a chamber like that with tons of FAE chances are you wont fuck them up by misting either way.
some use a syringe to just water the casing layer and not the mushrooms but thats kinda finicky at time consuming in a whole tub,
just get your mister down low and try to moisten the sub without splashing too much on the pan caps.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22771714 - 01/12/16 07:15 PM (8 years, 18 days ago)

The half gallon jar of wbs that I inoculated with the liquid culture and shook was fully colonized and I used it for a grain to grain transfer to 4 more half gallon jars of wbs and a quart jar and whatever size that little one is of rye.



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Edited by memberjockey (01/12/16 07:16 PM)


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22771922 - 01/12/16 07:52 PM (8 years, 18 days ago)

Quote:

memberjockey said:
Quote:

Fantastic Mr. Fox said:
Quote:

memberjockey said:
I will not patch my casing again unless specified by a higher power.:facepalm3:




lolol I told ya' not to patch them. :smirk:



:rolleyes: they looked cold.




lol comical


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Re: pan cyan agar clone LC G2G transfer [Re: Fantastic Mr. Fox]
    #22774931 - 01/13/16 03:15 PM (8 years, 18 days ago)

Quote:

memberjockey said:
The half gallon jar of wbs that I inoculated with the liquid culture and shook was fully colonized and I used it for a grain to grain transfer to 4 more half gallon jars of wbs and a quart jar and whatever size that little one is of rye.







Here's the jars 24 hours later.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22774957 - 01/13/16 03:21 PM (8 years, 18 days ago)

:fuckinawesome:
stellar stuff mj! i got some pans i need to deal with myself pretty quick here..fimicola cincs cams cyans biporus and trops


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22775031 - 01/13/16 03:41 PM (8 years, 18 days ago)

Shroom chicks rule! :bow2: We men are just a bunch of bunglers compare to them


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Re: pan cyan agar clone LC G2G transfer [Re: cronicr]
    #22775440 - 01/13/16 05:09 PM (8 years, 17 days ago)

I'd like to try some azurescens when I'm finished with this.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22775448 - 01/13/16 05:10 PM (8 years, 17 days ago)

i love azurs!


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22775482 - 01/13/16 05:18 PM (8 years, 17 days ago)

The same cakes have a few more pins on them & some are a little larger.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22778871 - 01/14/16 03:28 PM (8 years, 17 days ago)

More pins:crazy2:


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Edited by memberjockey (01/14/16 03:31 PM)


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22778892 - 01/14/16 03:34 PM (8 years, 17 days ago)

Quote:

memberjockey said:
Quote:

memberjockey said:
The half gallon jar of wbs that I inoculated with the liquid culture and shook was fully colonized and I used it for a grain to grain transfer to 4 more half gallon jars of wbs and a quart jar and whatever size that little one is of rye.







Here's the jars 24 hours later.




48 hours later
and 4 jars inoculated 4 days ago with a LC the far left jar I jostled right afterwards.


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22837999 - 01/29/16 10:37 AM (8 years, 2 days ago)

I have a new tub that was spawned on the 24 of January and has looked ready for a couple of days, I'm waiting a few more days at least before I case it.


Here an agar dish that I used to make my liquid culture that has grown back over the dish, I thought it looked pretty cool some think that pan doesn't branch out like this.


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Edited by memberjockey (01/29/16 10:44 AM)


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22838015 - 01/29/16 10:43 AM (8 years, 2 days ago)

My lesson is not case the tub too early and it won't go through the casing so quickly and to listen when I'm told not to patch it.:rolleyes:


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Re: pan cyan agar clone LC G2G transfer [Re: memberjockey]
    #22838490 - 01/29/16 01:42 PM (8 years, 2 days ago)

if you are waiting to case it blast it with some light while you wait


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