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OfflineIngSocTHC
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Tiger drop in spawn bag?
    #22261703 - 09/19/15 07:30 PM (8 years, 4 months ago)

Pretty straight forward. Looking to get into both agar and bags and monos again (assuming these are the preferred metohods of today) what I'm thinking is 1/1/1 meaning 1 drop, 1 bag(medium:5qts? Of rye), 1 tub(coir/verm)
Right now I run qt jars/coir/mini GH. Working very well, just looking to expand my horizons


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


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Offlinemushpunx
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22261799 - 09/19/15 07:53 PM (8 years, 4 months ago)

I would put your agar wedge to a master jar before putting it to a spawn bag.

I go wedge-quart master jar, G2G into 4qt spawn bag for a total of 5qts spawn, so one bag will spawn one normal size tub.


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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: mushpunx]
    #22261822 - 09/19/15 08:00 PM (8 years, 4 months ago)

Full circle or just a wedge? Up till now I have done g2ggg making 7jara, useing 2 as master and 5 for making tubs and with each jar I make I make 7-14 more jars and delete the process. I'm looking to get away from jars.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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Offlinemushpunx
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22261856 - 09/19/15 08:08 PM (8 years, 4 months ago)

You can use as much of a plate as you like, but definitely more economical to use a smaller wedge.

I will usually split a plate into 4 jars, thats my preference. One plate to 4 quarts into 4, 4qt bags into 4 tubs.

Spawn bags are great, and yea you can move away from jars but unless you are innoculating your bags via liquid innoculant you will probably want to at least knock up a pint jar as a master to G2G into spawn bag. 



  Spawn bags are pretty tricky without a flowhood though, I know lots of people do work with them in still air box but honestly if I didnt own a hood I would stick with jars


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Invisiblechampinhom
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22261858 - 09/19/15 08:08 PM (8 years, 4 months ago)

seems to me that it could be done. The above post advises you to go with your wedge--but I think you want to do a tiger drop, so substitute that for wedge--first to a jar of grain. Let that colonize and then pour the colonized grain into your bag of substrate. That is certainly a good way to get a multitude of colonization centers--so to say--spread through your sub. But you have to keep in mind that this advantage is offset by the fact that it took time for that spawn to form from the tiger drop in the jar.So, why not skip the middle step? Let the tiger drop do its work directly in the bag?

Well, you can go either way and I don't know you would be much the worse whichever you choose.


--------------------
My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said.

Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking.
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Those whom the gods would save, they dower with compassion. Mr. P.  Silocybin


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Offlinemushpunx
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Re: Tiger drop in spawn bag? [Re: champinhom]
    #22261881 - 09/19/15 08:15 PM (8 years, 4 months ago)

What is a tiger drop exactly? 

Before putting any culture to grain, you definitly want to make transfers untill you are sure your culture is clean


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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: champinhom]
    #22261883 - 09/19/15 08:15 PM (8 years, 4 months ago)

I use roughly 100ml worth of rye to each 600ml worth of grain jar or a 1-6 ratio. I understand why going from drop>qt of grain>5qt grain>mono. Just attempting to knock out the qt jars because I can't store them in the fridge(lots of friends come over to drink and I hate answering questions that doesn't concern them) do I figure keeping the glad mini in a bag in the fridge would draw less attention. Has any one done a drop in med sized bag with success? Of ao what was the colonization rate?


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22261890 - 09/19/15 08:17 PM (8 years, 4 months ago)

Mush: tiger drop is useing pastywhites agar tek and just dropping the entire agar circle into a jar. I figured if I put in a bag I could actually massage the myc around.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22261894 - 09/19/15 08:17 PM (8 years, 4 months ago)



--------------------
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Offlinemushpunx
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22261914 - 09/19/15 08:23 PM (8 years, 4 months ago)

Ive never put a wedge/plate directly into a spawn bag. I think when innoculating larger bags of spawn (5qts +) its definitely wise to inoculate a master jar first.

Why would you need to store your quart jars in the fridge? Why not simply put them in the same space you are going to let your spawn bags colonize in?


You could also blend the agar plate into liquid innoculant if you are comfortable with those techniques, and pour some of that into spawn bags (its been suggested to add some dry verm to the bottom of the spawn bag with this method).
If I was to skip master jars, this would be the method I would use.


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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: mushpunx]
    #22261923 - 09/19/15 08:26 PM (8 years, 4 months ago)

Why blend and not just drop?


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"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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Offlinemushpunx
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22262022 - 09/19/15 08:45 PM (8 years, 4 months ago)

I think dropping agar into too much grain might cause it to stall out. Don't quote me on that, because honestly I cant quite remember the reasoning behind it.
At this point I just know how I learned to prep spawn and I know how most cultivators that I trust prep spawn, and that is when preparing larger volume spawn bags you wanna inoculate via g2g or with liquid innoculants..

I mean by all means try it if you want to dude :shrug: it really isnt much more work to inoculate a pint jar as a master first though, you most likely won't be saving much/any colonization time dropping agar directly into the spawn bag


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Edited by mushpunx (09/19/15 08:49 PM)


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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: mushpunx]
    #22262051 - 09/19/15 08:52 PM (8 years, 4 months ago)

Thanks for your advice. Any good links you suggest reading?


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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Invisiblechampinhom
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Re: Tiger drop in spawn bag? [Re: champinhom]
    #22262153 - 09/19/15 09:09 PM (8 years, 4 months ago)

Quote:

champinhom said:
seems to me that it could be done. The above post advises you to go with your wedge--but I think you want to do a tiger drop, so substitute that for wedge--first to a jar of grain. Let that colonize and then pour the colonized grain into your bag of substrate. That is certainly a good way to get a multitude of colonization centers--so to say--spread through your sub. But you have to keep in mind that this advantage is offset by the fact that it took time for that spawn to form from the tiger drop in the jar.So, why not skip the middle step? Let the tiger drop do its work directly in the bag?

Well, you can go either way and I don't know you would be much the worse whichever you choose.




When I say here that you can do a tiger drop to a bag of substrate--I guess I sould amend that to say, no, you can't. You can do a tiger drop to a bag of grain. If the grain is the substrate then you can say that you can do a tiger drop to a substrate. But since subs are rarely grain, well, it wouldn't make sense doing a tiger to straw or coir. You need that colonized grain to get among the coir body or the straw. Otherwise...you would grow old waiting for anything to happen.


--------------------
My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said.

Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking.
Saul Bellow

“People are just cannibals unless they leave each other alone.” Doris Lessing

Those whom the gods would save, they dower with compassion. Mr. P.  Silocybin


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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: champinhom]
    #22262225 - 09/19/15 09:22 PM (8 years, 4 months ago)

OK so I might have used the wrong word? I don't get on the forums nearly enough to be effeciant with my dialogue.

But yes, I am looking to do a biopsy on a shroom, and drop that on to some agar via pastywhites tek. From there I am going to drop the entire (?) Agar gelly thing in a medium filter patch bag that holds roughly (5?)qts.

... From there I wish to monotub useing daimon5050s tek and la di da.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
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Invisibleelasticaltiger
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22262370 - 09/19/15 09:51 PM (8 years, 4 months ago)

Quote:

IngSocTHC said:
Pretty straight forward. Looking to get into both agar and bags and monos again (assuming these are the preferred metohods of today) what I'm thinking is 1/1/1 meaning 1 drop, 1 bag(medium:5qts? Of rye), 1 tub(coir/verm)
Right now I run qt jars/coir/mini GH. Working very well, just looking to expand my horizons




You are correct. You have realized the technique full circle. A puddle of agar at least 50% colonized (or less) can be transfered to a large spawn bag (or jar) and can inoculate a single substrate or more. The agar lets you see (mostly) visibly clean growth and the potential of such a huge amount of inoculate is virtually limitless.

Or you can simply use 1 ms petri to inoculate 1 spawn bag to inoculate 1 substrate.

It's how I do it every time.

You will go far.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: elasticaltiger]
    #22262517 - 09/19/15 10:16 PM (8 years, 4 months ago)

Thanks tiger, been reading a bit of your stuff. So what do you mean 50% or less? Meaning I don't have to wait 100% colonization on the top surface area or meaning the side exposed to air 100% and the side on the bottom not (making it 50%)

Am I safe to assume that 1 glad mini drop worth of myc is sufficient enough to colonize a medium bag of rye(is it roughly 5qts?) 
I apologize if I'm repeating, just know that things can get lost is translation and I'm more of a hands on learning type.
Thanks everyone so far


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


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Invisibleelasticaltiger
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22262571 - 09/19/15 10:27 PM (8 years, 4 months ago)

Letting the plate grow out fully is just a test. If you can successfully fully(1) colonize an agar plate with, clean(2) cultures repeatedly

1: (to the edge of the plate and beyond}
2: (apparently, as under scrutinizing inspection)

Edit: I didn't finish the thought because I was annotating, if you can get clean growth repeatedly to the edge of the plate without obvious contamination then you are almost winning the war, at that point, your clean cultures can go forever, mycology is not a single grow. Mycology is the ability to perpetuate life of mycelium over endless transfers and generations.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Edited by elasticaltiger (09/19/15 10:31 PM)


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OfflineIngSocTHC
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Re: Tiger drop in spawn bag? [Re: elasticaltiger]
    #22262800 - 09/19/15 11:22 PM (8 years, 4 months ago)

Very well articulated, at this point I consider myself fairly experienced grower. As you* said becoming a mycologist is a difference level. You're the man tiger, took me a minute to realize it's your tek I am discussing. Very honored.


--------------------
"Music your, music your the key.
Talk to who, plese talk to me.
Bring the voice of the rastaman
communicating to everyone" - Bob Marley


Edited by IngSocTHC (09/19/15 11:48 PM)


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Offlinemushpunx
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Re: Tiger drop in spawn bag? [Re: IngSocTHC]
    #22262838 - 09/19/15 11:34 PM (8 years, 4 months ago)

Tiger - so do you skip master jars? You drop agar wedge straight into large spawn bags? 

I hate G2G. Id much rather skip it myself. But I've always learned it was good practice to use a master to inoculate larger volumes of spawn


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