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InvisibleRaven44
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Bad genetics?? F4 amazonian culture ?? *DELETED*
    #22259590 - 09/19/15 10:17 AM (8 years, 4 months ago)

Post deleted by Raven44<p>Reason for deletion: blue moon syringes are bunk never ever use them. Do not use that guys products period.


Edited by Raven44 (11/17/15 11:57 PM)


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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Raven44]
    #22259765 - 09/19/15 11:23 AM (8 years, 4 months ago)

what?


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Offlineshellzenone
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Re: Bad genetics?? F4 amazonian culture ?? [Re: blackdust]
    #22259907 - 09/19/15 11:57 AM (8 years, 4 months ago)

From what I understand, every ms grow is like hitting g the reset button on genetics. Maybe you isolated some bad mycellium :shrug: it might be better to do a ms grow, and pick a fruit to clone. At least this way you know the strain you're working with is proven to fruit .


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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: shellzenone]
    #22259923 - 09/19/15 12:01 PM (8 years, 4 months ago)

maybe do 3 transfers, allow the plate to fruit, then clone the fruit on more agar that will then be used for growing?

Narrows done genetics, proves fruiting, allows enough genetic variation.
At least thats how I understood from what Whyte told me


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InvisibleRaven44
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Re: Bad genetics?? F4 amazonian culture ?? *DELETED* [Re: blackdust]
    #22260009 - 09/19/15 12:24 PM (8 years, 4 months ago)

Post deleted by Raven44

Reason for deletion: Dud



Edited by Raven44 (09/19/15 12:45 PM)


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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Raven44]
    #22260059 - 09/19/15 12:36 PM (8 years, 4 months ago)

oh god, new moon.

Quote:

What is "New Moon" ???

  "New Moon" is a formula that I invented for use in the lab to help keep master cultures and spores alive in a sterile environment for longer periods of time, preventing other molds and bacteria.
  Remember that even sterile hermetically sealed containers/media/bags/medical equipment and tools have an expiration date which means they are no longer sterile after that date. Lab professionals know that even the most sterile procedures in making a culture will eventually create bacteria. If a culture starts producing primordia the primordia produce bacteria, its just science. Well where does the bacteria come from?? It is created. We are dealing with living organisms...."Life finds a way"

  After years of working in labs transferring and re-invigorating cultures and at times dealing with contamination issues when I could least expect them or want them, I decided to create some kind of contaminant resistant liquid media to store cultures and spores in for prolonged use. Now I'll go ahead and make it clear here that this does not mean that our "old school" style of spore syringes have a risk of getting to you contaminated. Absolutely not. All our spores are covered under our 100% guarantee, and you have an option of still picking the "old school" spores for those of you that have grown to love our old school syringes.
  After testing different things during many years, the "New Moon" concept came about.

Containing 7 key ingredients (not including spores).


  About 99.9% organic and all lab grade ingredients that help stimulate and not harm, this formula can keep the spores sterile for a much longer period of time. In some rigorous tests I found that a "New Moon" syringe can actually be completely opened by pulling the plunger out the back, dumped in a kitchen bowl under a non-sterile environment and then poured back into the syringe and re-plugged and used without contaminants appearing on the substrate. (Do not attempt) At that point I decided to patent the concept and have been using it since on my own spore and culture syringes and now available to all our customers.

  I have recently read in forums by skeptics that this is just a Gimmick or a Trick to make people think that they are getting more spores. This is Untrue. Humans often criticize what they cannot understand. The "New Moon" has always been sold as a contaminant resistant spore syringe. Does it work? Yes, yes it does. Just ask people who have actually bought one.
  *This does not mean that you can get lazy or clumsy with your sterile procedures!* It's just a safeguard!

  Now, as far as costs go, A "New Moon" syringe is more than 3 times as expensive to make as a regular spore syringe. Time involved in preparation and in the making process of the "New Moon" is about 5 times that of regular spore syringes.

  So why do we sell them at the same price?... Well, To have the best spore syringes out there at an affordable price. Now based on other lab-work that I've done and charged for, I could reasonably price a "New Moon" spore syringe for as much as $75. Not for the spores (because the spores are abundant) but for the solution itself and the ingredients. We sell them for the same price as our old school syringe. Originally thought as an introductory price only. Its popularity soon blew up and I decided to keep it at that extremely low price, but that could change at any time.

  OTHER QUALITIES NOTICED:

*FASTER GERMINATION TIMES
*FASTER COLONIZATION TIMES
*HELPS PREVENT FOREIGN MOLDS AND BACTERIA ON SUBSTRATES AT INOCULATION SITES EVEN BEFORE MYCELIUM BEGINS TO GROW
*HELPS PREVENT BACK CONTAMINATION OF THE SYRINGE BEFORE AND AFTER USE, GREAT FOR STORE-ABILITY
*MYCELIUM TENDS TO BE MORE AGGRESSIVE AND LAST LONGER IN ITS VITALITY - ENCOURAGES HEALTHY GROWTH!
*CULTURES AND SPORES STORED IN "NEW MOON" SOLUTION OR MEDIA ALWAYS OUTLAST ANY OTHER MEDIAS OR SOLUTIONS

So why is it called "New Moon"? Because of its radical change after you shake it. It turns completely black. So much so that no light can pass through it even if you hold it up to a light bulb. Just like our moon gets when it goes from full and bright to the new moon which is completely invisible in darkness.


NEW MOON SPORE SYRINGES ARE HARDLY SUITED FOR BRIGHT-FIELD MICROSCOPY DUE TO ITS DARKNESS.




:lolsy:


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InvisibleRaven44
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Re: Bad genetics?? F4 amazonian culture ?? *DELETED* [Re: blackdust]
    #22260114 - 09/19/15 12:51 PM (8 years, 4 months ago)

Post deleted by Raven44

Reason for deletion: Dud



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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Raven44]
    #22260176 - 09/19/15 01:03 PM (8 years, 4 months ago)

you need better genetics
stay away from that site


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InvisibleRaven44
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Re: Bad genetics?? F4 amazonian culture ?? *DELETED* [Re: blackdust]
    #22260312 - 09/19/15 01:41 PM (8 years, 4 months ago)

Post deleted by Raven44

Reason for deletion: Dud



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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Raven44]
    #22260317 - 09/19/15 01:43 PM (8 years, 4 months ago)

PM Pastywhyte,
he is your genetic man


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OfflineKizzle
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Re: Bad genetics?? F4 amazonian culture ?? [Re: blackdust]
    #22260321 - 09/19/15 01:45 PM (8 years, 4 months ago)

Quote:

I know what a contaminated jar looks like when it doesn't recover...



The appearance really varies. If some or all grains are still showing absolutely no sign of recovery after 48 hours I would consider that a sign of contamination regardless of whether they recolonized eventually. That's assuming they were fully colonized before the shake.


--------------------


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InvisibleRaven44
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Re: Bad genetics?? F4 amazonian culture ?? *DELETED* [Re: Kizzle]
    #22260459 - 09/19/15 02:29 PM (8 years, 4 months ago)

Post deleted by Raven44

Reason for deletion: Dud



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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Raven44]
    #22260516 - 09/19/15 02:44 PM (8 years, 4 months ago)

START NEW
why do you even want iso?
They perform far less 9/10
WHat is it you are trying to do? Experiment? Learn genetics? or grow shrooms?

If you are experimenting or learning genetics then by all means keep the genetics for your own purposes. On the other hand, if you are trying to grow bulk shrooms then you need to stop what you are doing and start new.


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InvisiblePastywhyteMDiscord
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Kizzle]
    #22260711 - 09/19/15 03:23 PM (8 years, 4 months ago)

Okay OP i got your :pm: First there are no pics of anything. Forgive my bluntness but you don't know enough of whats happening to describe it to us. Its like a high school student trying to perform heart surgery by texting questions to the surgeon.

Here are a few points I will toss out while you get some pics up.

First it could be genetics. Poor care of a lineage can degrade the genetics over many generations. This is least likely. Even the worst lineage can still produce a good culture throught careful isolation.

Second isolates are pointless for you at this stage. Like dust said get some clones going so you can learn about what you're looking for and have a grow at the same time.

Third, I suspect Kizzle is most on point here. Many bad results are blamed on genetics when contamination or poor conditions are to blame. Grain jars stall for 4 reasons.  No GE, improper moisture levels (too dry or too wet), senscence, and contamination. Sounds like a case of bacteria to me.

Pics man. This is all pointless without them. Also break your posts up into paragraphs and tell us only what we need. Giant word castles make me want to reply with TL; DR.


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InvisibleRaven44
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Re: Bad genetics?? F4 amazonian culture ?? *DELETED* [Re: Pastywhyte]
    #22260984 - 09/19/15 04:33 PM (8 years, 4 months ago)

Post deleted by Raven44

Reason for deletion: Dud



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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Raven44]
    #22261008 - 09/19/15 04:38 PM (8 years, 4 months ago)

have you verified that you are working with cube mycelium?


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InvisibleRaven44
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Re: Bad genetics?? F4 amazonian culture ?? *DELETED* [Re: Raven44]
    #22261224 - 09/19/15 05:33 PM (8 years, 4 months ago)

Post deleted by Raven44

Reason for deletion: Dud



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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Raven44]
    #22261291 - 09/19/15 05:51 PM (8 years, 4 months ago)

i am not the guy to help.
this is all well beyond me


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InvisiblePastywhyteMDiscord
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Re: Bad genetics?? F4 amazonian culture ?? [Re: blackdust]
    #22261355 - 09/19/15 06:09 PM (8 years, 4 months ago)

I'm betting those syringes are not even spores. F4 cultures suggests either 4th generation from wild or crossed genetics (which will be poorly stabilized at that point) or a name of a live culture. Given your description I'm skeptical that you even have cube mycelium or that its clean or healthy. Thats why i want pics. If youre growing trich or a bacterial mess then you should scrap the whole thing.

The new moon syringes are the ones that are blackened with activated charcoal if I remember correctly. Many suspect its live culture in there. Tomentose myc is not a problem, I have many tomentose clones that still fruit prolifically. But if its tomentose because of senescense or simply because its mold you will not be growing much from it.

Amazon is a classic cube, one of the earliest and I would consider it to be decent stock. The wikidzon I am so fond of are actually amazon originally. I think at this point you would be best served to bite the bullet and buy some good spores from a reputable vendor.


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Invisibleblackdust
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Re: Bad genetics?? F4 amazonian culture ?? [Re: Pastywhyte]
    #22261422 - 09/19/15 06:24 PM (8 years, 4 months ago)

thats why i asked if he has verified that it is cube myclium


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