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DaveyJones6911
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any idea what this is?
#22257772 - 09/18/15 07:43 PM (8 years, 4 months ago) |
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a Ps. cyanescens syringe on a pf cake gave me this:

anyone have an idea what this is?
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zZZz
jesus


Registered: 12/28/07
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dont know what it is unfortunately, im monitoring this thread tho
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TheHulk69
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Re: any idea what this is? [Re: zZZz]
#22257950 - 09/18/15 08:22 PM (8 years, 4 months ago) |
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Lol I thought you meant that square logo thingy for a sec.
I assume that what you're asking about is the yellow liquid, this is just mycelium secretions, nothing to worry about it's fairly common
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zZZz
jesus


Registered: 12/28/07
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Re: any idea what this is? [Re: TheHulk69]
#22257971 - 09/18/15 08:28 PM (8 years, 4 months ago) |
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that;s what i thought as well, but seems a bit early dont u think?.. his jar barely even looks colonized..
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DaveyJones6911
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Re: any idea what this is? [Re: zZZz]
#22258097 - 09/18/15 08:54 PM (8 years, 4 months ago) |
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i can't seem to get a good picture of it. but doesn't really look like mycelium. it looks dry and is off white, in some places kind of pink-ish, not the bright shiny myc like i'm used to seeing with cubensis.
the way it looks to my eye is that it has no real mass to it. like if i were to open the jar and touch it it would crumble into dust.
also this is one of 6 jars i started at 09/01. 4 other jars are showing no sign of life yet.
anyway i made a pic with a cubensis jar next to it to get a better idea of the colour:
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DaveyJones6911
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i found a pic of actual Ps. cyanescens: http://www.shroomery.org/10820/mycelium-growth-on-cardboard
looking at what i have here i could not think of anything less like what i see in the picture. except, it does spread in a way similar to mycelium. i am starting to wonder if there was a packaging issue and i got the wrong species by mistake. however, just to see what would happen i also made some jars with sterilised wood chips and injected some of the same spores into those. and it seems i have something going on in one of those jars:
 
 
the yellow in the other jar is definitely not metabolites. it is dull like the rest of the 'mycelium' or whatever it is. i can't seem to get a good pic of it due to the plastic of the jar. best one so far:
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confuzzed
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I too a monitoring this thread.
I'm interested in your results. I agree that it does not look like cyans...
I wonder if it's wood loving either. Keep us posted!!!
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micro
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Re: any idea what this is? [Re: confuzzed]
#22269819 - 09/21/15 02:45 PM (8 years, 4 months ago) |
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yeah, i think it's a bit early to tell with the woodchips
hah, i thought that was standing water but it's the shape of the glass
what temp you have those colonising at?
oh wait, cyans
i thought you said azurescens for some reason
hmm... idk, if it's not p. cyans mycelium then what is it
have you opened it yet? it looks colonised, at least from what i see
wonder if it smells mushroomy
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DaveyJones6911
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Re: any idea what this is? [Re: micro]
#22270283 - 09/21/15 04:26 PM (8 years, 4 months ago) |
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Quote:
micro said: yeah, i think it's a bit early to tell with the woodchips
hah, i thought that was standing water but it's the shape of the glass
what temp you have those colonising at?
oh wait, cyans
i thought you said azurescens for some reason
hmm... idk, if it's not p. cyans mycelium then what is it
have you opened it yet? it looks colonised, at least from what i see
wonder if it smells mushroomy
nah it's nowhere near complete. you can see pf mix on the right in that last picture.
and i am colonising at 22-25C / 72-77F.
i got the syringe from azarius, they sell a few different species. incl: P. ovoideocystidiata, Pan. cyan, Pan. goliath, P. semilaceata and several sclerotia producers. but they get their syringes from another company i think, so it really could be anything as i am speculating the syringe was labelled wrong.
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DaveyJones6911
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btw i also have azure spawn going, it is at 18C to 22C and doing quite well. the 'cyan' didn't seem to do well in that spot so i moved them to the other spot where i have my cubensis and they seem to be picking up now.
do cyans and azures require different temps?
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micro
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cyans:
spawn run: 65-75F cropping: 50-60F
azures I can't find in stamets but i think they are about the same
so that sounds about right
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DaveyJones6911
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Re: any idea what this is? [Re: micro]
#22272694 - 09/22/15 05:37 AM (8 years, 4 months ago) |
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well my azure spawn is certainly doing well at those temps. whatever this is, it's definitely not cyanescens.
and the vendor agrees and offered to send me a replacement. 
i will continue to try to grow whatever this is out to see what it is. right now i have standard brf jars and the wood chips going but i still have a tiny bit of spore solution left. i also have cow manure that i could try, and as a D&D crafting hobbyist i of course have an ample supply of cardboard.
can you guys suggest any other potential substrates i could try that do not require a pressure cooker? or any other kind of experiment i could try?
i have a big pot and by way of experiment i think i managed to almost sterilise some rye. (they all turned gray so uniformly as to suggest something it carried germinated, but not one trace of anything green and/or nasty besides the gray stuff) i will be making another attempt at that some time soon.
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DaveyJones6911
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i found another jar with a spot of pseudelium (tm) and it is starting to produce the same yellow in the center of it. here's another pic of the biggest one with some white paper for contrast:
 i also noticed one of the other jars had a lot of pseudelium developing right at the dry verm layer:
 i injected all of them with a reserved squirt right at the centre of the cakes, yet here the pseudelium seems to have a particular fondness for the edge of the dry verm layer for some reason.
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azur
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Aspergillus flavus
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DaveyJones6911
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Re: any idea what this is? [Re: azur]
#22277770 - 09/23/15 08:16 AM (8 years, 4 months ago) |
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so the vendor was out of cyans, and they agreed to send me a syringe of P. ovoideocystidiata. 
Props to Azarius for excellent service.
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DaveyJones6911
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Re: any idea what this is? [Re: azur]
#22277910 - 09/23/15 09:13 AM (8 years, 4 months ago) |
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Quote:
azur said: Aspergillus flavus
i dunno, i injected these jars on sept. 1st. i would expect asperagillus to be quicker then this. also doesn't that 'auto flower'? i am not seeing anything that suggests spores.
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micro
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sepedonium?
probably that or the good stuff
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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DaveyJones6911
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Re: any idea what this is? [Re: micro]
#22279014 - 09/23/15 02:04 PM (8 years, 4 months ago) |
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Quote:
micro said: sepedonium?
probably that or the good stuff
googled it, and this seems plausible. from the look of it and the way it infects mushrooms themselves it might be that there is sepedonium in the mix which infects the mycelium as it grows. that would explain what i am seeing in the jars.
if this is what it is, is there any chance of isolating some healthy myc from the mix? i still have about 1cc of spore solution. but i don't have a PC so i'm not sure what my options with agar are.
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Mad Season
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Lots of labs microwave agar with no over boiling for 10 minutes with success. Just a thought the problem is you gotta mmake sure there's no chunks. It's much easier to sterilize a liquid over a solid
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micro
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Re: any idea what this is? [Re: Mad Season]
#22280646 - 09/23/15 08:28 PM (8 years, 4 months ago) |
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they microwave it to make the agar into solution
then autoclave it
after that, once it is cooled a bit they'll usually add ampicilin
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DaveyJones6911
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Re: any idea what this is? [Re: micro]
#22280902 - 09/23/15 08:54 PM (8 years, 4 months ago) |
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so steam sterilising agar is not an option i take it?
anyway i came across this: http://www.shroomery.org/8514/Agar-substitute i think i will give this a try.
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micro
bunbun has a gungun



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anything is an option
it just depends what you want out of it
i always tried to stay contaminate free so no spores all over
maybe had <=5%
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confuzzed
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Re: any idea what this is? [Re: micro]
#22293517 - 09/26/15 01:05 PM (8 years, 4 months ago) |
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Ovoids !!!!
U will enjoy these! Are you fruiting outdoors? I usually dose around 2 grams with these and have a great experience! They aren't as long lasting as cubes for me, but are more enjoyable!
Mycelium is supposed to be easy to colonize and resistant, but many have trouble fruiting...
Good luck!
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DaveyJones6911
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Re: any idea what this is? [Re: confuzzed]
#22293852 - 09/26/15 02:09 PM (8 years, 4 months ago) |
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Quote:
confuzzed said: Ovoids !!!!
U will enjoy these! Are you fruiting outdoors? I usually dose around 2 grams with these and have a great experience! They aren't as long lasting as cubes for me, but are more enjoyable!
Mycelium is supposed to be easy to colonize and resistant, but many have trouble fruiting...
Good luck! 
thanks  they are supposed to fruit in spring/early summer so i figured they would be a nice addition to the azure spawn i have going.
and yeah i am making an outdoor patch.
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stevo

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Post deleted by stevo
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DaveyJones6911
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Re: any idea what this is? [Re: stevo]
#22342121 - 10/06/15 06:34 PM (8 years, 3 months ago) |
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getting funkier:

 
i am guessing some kind of bacteria is infecting the mycelium?
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confuzzed
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Are they stalled out? Looks like it to me.
Bacteria usually spreads like a blob or a slime when on a solid substrate... Do the spots look fuzzy/hairy/fibrous?
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enlightened seed
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Re: any idea what this is? [Re: confuzzed]
#22343711 - 10/07/15 12:42 AM (8 years, 3 months ago) |
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soak it in hydrogen peroxide for a while then try to fruit it? it might work it might not but it is better than throwing in the trash. you might learn something. just a thought.
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stevo

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Post deleted by stevo
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DaveyJones6911
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Re: any idea what this is? [Re: stevo]
#22344306 - 10/07/15 08:08 AM (8 years, 3 months ago) |
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well actually i still have a little squirt of the syringe left and i am just getting into agar, so maybe i can get some clean myc that way. so i think ill dump them in my garden with some woodchips and see what happens.
the cakes are not stalled out, and some of the yellow spots are cottony, but now a lot of yellow goo is also showing up.
in any case this confirms a dirty syringe (2 in a row actually) and contamination, not the wrong species.
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confuzzed
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Re: any idea what this is? [Re: stevo]
#22345031 - 10/07/15 12:07 PM (8 years, 3 months ago) |
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Quote:
stevo said: I would not recommend using h2o2. Its true that it kills the mold back but also really fucks the good mycelium up badly, assuming there is any. If that is ovoid myc dirty or not Id say you have about a 0% chance of fruiting off of a cake like that. What I would do is mix it up with some mulch and put it in a well aired out tub or flower pot outdoors or even on the ground. It may sound creepy but all of the species youve mentioned are very aggressive and will outlive most contaminations when put in the right environment. Good luck
I agree with him. The h202 will possibly work but will stall the myc out and inhibits it's vigor if soaked and if I thought it was cobweb and u could spray a layer of h202 on then give it fae I would say go for it, but that is not cobweb...
I dunno what it is 
Also isn't this ps cyan myc? I thought ovoid was coming in the mail? Doesn't matter either way... "Cottony" isn't good ... If it sporulating it will be hard not to transfer over both bad and good....
If it's multiple cakes u could experiment and do h202 and do what Stevo said and see which one works
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DaveyJones6911
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Re: any idea what this is? [Re: confuzzed]
#22345167 - 10/07/15 12:35 PM (8 years, 3 months ago) |
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meh i thought there might be a chance they just sent a mislabelled syringe, but this is clearly a contam so i don't think i will do any experiments with these jars except that i might as well bury them in my garden instead of my trashbin.
and yeah it was Ps. cyans, the ovoid was sent as a replacement cuz they were out of cyans.
like i said i am getting into agar and i still have a few drops left in the syringe. enough for several attempts. with luck i might be able to get something out of that. if not so be it. i still have the ovoids and some healthy azure spawn so i really have nothing to complain about.
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confuzzed
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It happens
Hope those few left over drops are loaded with spores! Good luck !!!
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DaveyJones6911
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Re: any idea what this is? [Re: confuzzed]
#22527837 - 11/15/15 02:51 PM (8 years, 2 months ago) |
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well here's the results of my attempt with agar:
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zZZz
jesus


Registered: 12/28/07
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looks like u've got the same goo on the agar..
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DaveyJones6911
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Re: any idea what this is? [Re: zZZz]
#22536419 - 11/17/15 08:21 AM (8 years, 2 months ago) |
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i don't seem to have anything else there either.
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