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InvisibleDaveyJones6911
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any idea what this is?
    #22257772 - 09/18/15 07:43 PM (8 years, 4 months ago)

a Ps. cyanescens syringe on a pf cake gave me this:



anyone have an idea what this is?


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InvisiblezZZz
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Re: any idea what this is? [Re: DaveyJones6911]
    #22257910 - 09/18/15 08:15 PM (8 years, 4 months ago)

dont know what it is unfortunately, im monitoring this thread tho :strokebeard:


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OfflineTheHulk69
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Re: any idea what this is? [Re: zZZz]
    #22257950 - 09/18/15 08:22 PM (8 years, 4 months ago)

Lol I thought you meant that square logo thingy for a sec.

I assume that what you're asking about is the yellow liquid, this is just mycelium secretions, nothing to worry about it's fairly common


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InvisiblezZZz
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Re: any idea what this is? [Re: TheHulk69]
    #22257971 - 09/18/15 08:28 PM (8 years, 4 months ago)

that;s what i thought as well, but seems a bit early dont u think?.. his jar barely even looks colonized.. :strokebeard:


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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: zZZz]
    #22258097 - 09/18/15 08:54 PM (8 years, 4 months ago)

i can't seem to get a good picture of it.
but doesn't really look like mycelium.
it looks dry and is off white, in some places kind of pink-ish,
not the bright shiny myc like i'm used to seeing with cubensis.

the way it looks to my eye is that it has no real mass to it.
like if i were to open the jar and touch it it would crumble
into dust.

also this is one of 6 jars i started at 09/01.
4 other jars are showing no sign of life yet.

anyway i made a pic with a cubensis jar next to it
to get a better idea of the colour:



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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: DaveyJones6911]
    #22269575 - 09/21/15 01:56 PM (8 years, 4 months ago)

i found a pic of actual Ps. cyanescens: http://www.shroomery.org/10820/mycelium-growth-on-cardboard

looking at what i have here i could not think of anything less like what i see in the picture.
except, it does spread in a way similar to mycelium. i am starting to wonder if there was a packaging
issue and i got the wrong species by mistake. however, just to see what would happen i also made some jars
with sterilised wood chips and injected some of the same spores into those.
and it seems i have something going on in one of those jars:



the yellow in the other jar is definitely not metabolites. it is dull like the rest of the 'mycelium' or whatever it is.
i can't seem to get a good pic of it due to the plastic of the jar.
best one so far:


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Offlineconfuzzed
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Re: any idea what this is? [Re: DaveyJones6911]
    #22269795 - 09/21/15 02:40 PM (8 years, 4 months ago)

I too a monitoring this thread.

I'm interested in your results.
I agree that it does not look like cyans...

I wonder if it's wood loving either.
Keep us posted!!!


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Invisiblemicro
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Re: any idea what this is? [Re: confuzzed]
    #22269819 - 09/21/15 02:45 PM (8 years, 4 months ago)

yeah, i think it's a bit early to tell with the woodchips

hah, i thought that was standing water but it's the shape of the glass

what temp you have those colonising at?

oh wait, cyans

i thought you said azurescens for some reason

hmm... idk, if it's not p. cyans mycelium then what is it

have you opened it yet? it looks colonised, at least from what i see

wonder if it smells mushroomy


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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: micro]
    #22270283 - 09/21/15 04:26 PM (8 years, 4 months ago)

Quote:

micro said:
yeah, i think it's a bit early to tell with the woodchips

hah, i thought that was standing water but it's the shape of the glass

what temp you have those colonising at?

oh wait, cyans

i thought you said azurescens for some reason

hmm... idk, if it's not p. cyans mycelium then what is it

have you opened it yet? it looks colonised, at least from what i see

wonder if it smells mushroomy




nah it's nowhere near complete.
you can see pf mix on the right in that last picture.

and i am colonising at 22-25C / 72-77F.

i got the syringe from azarius, they sell a few different species.
incl: P. ovoideocystidiata, Pan. cyan, Pan. goliath, P. semilaceata and several sclerotia producers.
but they get their syringes from another company i think, so it really could be anything as i am speculating the syringe was labelled wrong.


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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: DaveyJones6911]
    #22270293 - 09/21/15 04:29 PM (8 years, 4 months ago)

btw i also have azure spawn going, it is at 18C to 22C and doing quite well.
the 'cyan' didn't seem to do well in that spot so i moved them
to the other spot where i have my cubensis and they seem to be picking up now.

do cyans and azures require different temps?


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Invisiblemicro
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Re: any idea what this is? [Re: DaveyJones6911]
    #22270602 - 09/21/15 05:48 PM (8 years, 4 months ago)

cyans:

spawn run: 65-75F
cropping: 50-60F

azures I can't find in stamets but i think they are about the same

so that sounds about right


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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: micro]
    #22272694 - 09/22/15 05:37 AM (8 years, 4 months ago)

well my azure spawn is certainly doing well at those temps.
whatever this is, it's definitely not cyanescens.

and the vendor agrees and offered to send me a replacement. :smile:

i will continue to try to grow whatever this is out to see what it is.
right now i have standard brf jars and the wood chips going but i still have a tiny bit
of spore solution left.
i also have cow manure that i could try, and as a D&D crafting hobbyist i of course have an ample supply of cardboard.

can you guys suggest any other potential substrates i could try that do not require a pressure cooker?
or any other kind of experiment i could try?

i have a big pot and by way of experiment i think i managed to almost sterilise some rye.
(they all turned gray so uniformly as to suggest something it carried germinated, but not one trace of anything
green and/or nasty besides the gray stuff) i will be making another attempt at that some time soon.


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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: DaveyJones6911]
    #22274853 - 09/22/15 04:06 PM (8 years, 4 months ago)

i found another jar with a spot of pseudelium (tm) and it is starting to produce the same yellow in the center of it.
here's another pic of the biggest one with some white paper for contrast:

i also noticed one of the other jars had a lot of pseudelium developing right at the dry verm layer:

i injected all of them with a reserved squirt right at the centre of the cakes, yet here the pseudelium seems to have a particular fondness for the edge of the dry verm layer for some reason.


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Invisibleazur
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Re: any idea what this is? [Re: DaveyJones6911]
    #22276263 - 09/22/15 08:37 PM (8 years, 4 months ago)

Aspergillus flavus


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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: azur]
    #22277770 - 09/23/15 08:16 AM (8 years, 4 months ago)

so the vendor was out of cyans, and they agreed to send me a syringe of P. ovoideocystidiata. :smile:

Props to Azarius for excellent service. :smile:


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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: azur]
    #22277910 - 09/23/15 09:13 AM (8 years, 4 months ago)

Quote:

azur said:
Aspergillus flavus




i dunno, i injected these jars on sept. 1st.
i would expect asperagillus to be quicker then this.
also doesn't that 'auto flower'?
i am not seeing anything that suggests spores.


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Invisiblemicro
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Re: any idea what this is? [Re: DaveyJones6911]
    #22278531 - 09/23/15 12:20 PM (8 years, 4 months ago)

sepedonium?

probably that or the good stuff


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InvisibleDaveyJones6911
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Re: any idea what this is? [Re: micro]
    #22279014 - 09/23/15 02:04 PM (8 years, 4 months ago)

Quote:

micro said:
sepedonium?

probably that or the good stuff




googled it, and this seems plausible.
from the look of it and the way it infects mushrooms themselves
it might be that there is sepedonium in the mix which infects the mycelium
as it grows. that would explain what i am seeing in the jars.

if this is what it is, is there any chance of isolating some healthy myc from the mix?
i still have about 1cc of spore solution. but i don't have a PC so i'm not sure
what my options with agar are.


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InvisibleMad Season
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Re: any idea what this is? [Re: DaveyJones6911]
    #22279087 - 09/23/15 02:23 PM (8 years, 4 months ago)

Lots of labs microwave agar with no over boiling for 10 minutes with success. Just a thought :shrug: the problem is you gotta mmake sure there's no chunks. It's much easier to sterilize a liquid over a solid


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Invisiblemicro
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Re: any idea what this is? [Re: Mad Season]
    #22280646 - 09/23/15 08:28 PM (8 years, 4 months ago)

they microwave it to make the agar into solution

then autoclave it

after that, once it is cooled a bit they'll usually add ampicilin


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