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Marshall
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Registered: 07/03/13
Posts: 50
Last seen: 8 years, 3 months
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PF Fischer Tek
#22257087 - 09/18/15 05:04 PM (8 years, 4 months ago) |
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This is my personally constructed technique. It is based off hundreds of observations through numerous methods.
This is my first write-up, and it is the first version. Please let me know (kindly) any questions, comments, concerns, objections, and/or suggestions. I prefer to keep it in Google docs for organization and distribution reasons, so here is the link to my write up.
PF Fischer Tek
Enjoy
Edited by Marshall (10/01/15 10:47 AM)
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Marshall
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Registered: 07/03/13
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Last seen: 8 years, 3 months
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Re: PF Fischer Tek [Re: Marshall]
#22257838 - 09/18/15 07:58 PM (8 years, 4 months ago) |
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Please notify my if the link to my document doesn't work. Its Google docs, so it should work for most.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: PF Fischer Tek [Re: Marshall]
#22258107 - 09/18/15 08:56 PM (8 years, 4 months ago) |
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Quote:
Potency Numerous studies have been produced, proving introduction of simpler tryptamines leads to a higher alkaloid content within the mycelium and fruiting bodies. L-Tryptophan is the most common, cost effective, abundant tryptamine available, and can be found in countless foods compatible with ‘dung loving’ fungi.
a.) that's not a tryptamine; the umbrella term would be amino acid b.) tryptophan will likely not cause an increase in potency and might even cause a decrease
b) because it initiates a self-feedback downregulation of the tryptophan dearboxylase which turns tryptophan to tryptamine. if this is the case, more tryptophan isn't going to help much. precursors upstream can increase potency (also causing a disproportionate percentage of psilocin vs. psilocybin). see here and here.
Quote:
Certain strains favor warmer, sub-tropical temperatures, and growth typically peaks around 82f incubating, and 78f fruiting. While this may be ideal parameters for fungal growth, alkaloid formation does not speed up. If you maintain lower temperatures during incubation and fruiting (75f incubating and 65f fruiting) you allow for more time for alkaloid formation. It adds about a week to the process, but dramatically increases potency.
[citation needed]
Aside from that (and I just skimmed through) but this seems like the PF tek just with more verbiage and a glove box. It still uses jars (which are far from ideal, IMO), a terrarium and the same major substrate recipe. I don't think the glove box is necessary and it seems to have the same major flaws as the original one.
Like... I understand the reason for the vermiculite but I never understood the reason behind the vermiculite, water and brown rice flour mixture when you can honestly just go to the grocery store, buy a huge cheap bag of parboiled rice and cook it using 3/4 of the water or whatever it calls for (it has been a while but I think that's right). No mixing and worrying about evenness and IME the rice colonises faster anyway.
Also, I think jars are flawed for several reasons:
- You can't break them up and mix around the mycelium after it's ~ 10% colonised. This will speed up colonisation making it harder for contaminants to take hold, not to mention less waiting.
- You can't smell bacteria or yeast as easily as you can with filter-patch bags, where you just gotta smell near the filter
- You can't make more inoculum with the jar alone. With bags you can inject sterile water and take it back up in a syringe with a good gauge needle
- Jars hold a lot less than a filter patch bag can
- The bags have a "self-healing" septum-type inoculation site, which is good for keeping things clean (I used rubbing alcohol on the inoculation site first)
Also, I've always found casing to do more good than a terrarium-type setup. Not to mention you don't have to worry about a terrarium then; plastic bins covered in plastic or saran wrap (depending on size) seemed to work just fine for casings since the casing itself holds the moisture in.
That's just my opinion, but is why I've never really been a fan of the "PF Tek" in the first place. It doesn't seem like the easiest method, and it certainly isn't the most efficient either, in any regard.
But regardless, it seemed like the PF tek with some nuances that may or may not help.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Marshall
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Registered: 07/03/13
Posts: 50
Last seen: 8 years, 3 months
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Re: PF Fischer Tek [Re: micro]
#22260106 - 09/19/15 12:47 PM (8 years, 4 months ago) |
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Until you attempt this technique, how can you bash it like that? This technique DOES produce higher potency, it DOES achieve a consistent yield of 200% BE, it's cheaper and simpler than many, how can you argue with that? Everyone has different ways of finding their success. There is not one way to do things. If I can maintain simplicity, maximize efficiency, potency,and yield, while minimizing cost, that is certainly the path I will choose.
Regardless of studies objecting other studies, potency IS increased by something(s) in this technique, I can assure you of that 
My only intention of distributing this information is to provide my observations for others to build their own techniques.
P.S. A crappy PF Cake recipe has roughly 45 grams of dry nutrients in it, so a 200% BE would produce 90 grams of wet fruits.
50 jars x 90g = 4500g fresh mushrooms
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: PF Fischer Tek [Re: Marshall]
#22260148 - 09/19/15 12:58 PM (8 years, 4 months ago) |
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i thought the bullet points were a good way to delineate the disadvantages of jars
re: potency -- not sure if it's placebo effect, confirmation bias or maybe you did something all the other people didn't who tried that but it's my nature to be sceptical and given the facts it makes no sense to me. i explained why
also, casing
if i ever start growing again i'll give it a try though
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Marshall
Stranger
Registered: 07/03/13
Posts: 50
Last seen: 8 years, 3 months
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Re: PF Fischer Tek [Re: micro]
#22264296 - 09/20/15 11:13 AM (8 years, 4 months ago) |
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What is with the Shroomery? This place is full of arrogant punks, I am blown away. This is a forum, where people come to share and exchange information and experience, not a place to make uneducated assumptions about a process you know nothing about until you have attempted it. Scepticism is fine, but you make a complete ass of yourself judging an outcome of something you have not even attempted. Just because you THINK you found the one and only perfect method, doesn't mean that others cannot find success alternatively.
The leaders of this website are foolish, this website is a poisonous scourge upon mycology. Shame on anyone who doesn't use this forum to only further mycological understanding and awareness.
This is a high school playground and I promise some day the managers of this forum will be held responsible for what takes place on this forum;look around, there are many others that are very displeased, and cyberspace is an infinitely powerful tool.
GM's: delete this post, ban my account, do as you will, but this will not go on forever.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: PF Fischer Tek [Re: Marshall]
#22265201 - 09/20/15 02:20 PM (8 years, 4 months ago) |
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QQ
So, you posted a thread NOT wanting a response?
I don't need to follow something step-by-step to comment on the various facets (most or all in which I have experience).
There is no "special step-by-step method" that will ever prove superior; it's the sum of the averages and personal preference / convenience. For example, if someone is having a lot of contamination, maybe the SAB is good? Maybe they live in an area with a lot of airborne spores (like a farm, for example).
Don't QQ over a response though. If you don't like it, why not address the various points? Complaining about the "people who run this site" and attacking a post without any content or specific topic(s) isn't constructive at all. If you want to show tryptophan increases potency why not grow a controlled experiment and then titrate with Ehrlich's reagent for example (keep in mind other factors such as mushroom size would need to be taken into account since it can effect potency, as well). All I was saying is it has been done before.
I've spent a while trying to dig up the citations from that paper but it's all broken links (I'm not *too* shocked since it's 12 years old). I found stuff on human metabolic pathways (mainly at 5-HT to serotonin) but nothing I cited and nothing relevant. If course, I had journal access back then. Still, can't hurt to repeat experiments but be analytical about it. There's nothing scientific about saying "I tripped really hard off these..."
Just sayin'
Oh, funny thing is there was a post from like 2004 I apparently posted in about DPT + substrate. Nothing conclusive though. However.... I ran by a paper the claimed *it was* hydroxylated. Cool B)
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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