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Offlinebinko
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Registered: 09/18/15
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Agar to Lc
    #22256071 - 09/18/15 12:50 PM (8 years, 4 months ago)

hi i was wondering how to best transfer my agar plate free from contamination to my Lc solution. i dont want to open the can to my lc for the contamination risk.
any ideas?


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OfflinetaGyo
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Re: Agar to Lc [Re: binko]
    #22256116 - 09/18/15 01:01 PM (8 years, 4 months ago)

You're gonna have to open it in an SAB and drop a wedge of the agar in there.

:goodluck:


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Offlineaerow.thefox
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Re: Agar to Lc [Re: taGyo]
    #22256223 - 09/18/15 01:25 PM (8 years, 4 months ago)

im trying my first agar right now. if im not mistaken the best way is to

equalize pressure in lc first

prepare your cutting

pop and slide the lid only a little

scrape the wedge onto rim of jar, making sure to keep your angle as verticle as possible to ensure contact only to the inside rim and not to where the metal band screws on.

then re-lid and shake.


you might could prepare your cutting  with the dish on the lc jar. that way after taking the cutting, the motion of closing the agar dish can easily and swiftly turn to opening the lc jar. assuming ur using widemouth lc jar..
or i could be totally wrong and a tc might shoot me down.  :shrug:


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OfflinetaGyo
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Re: Agar to Lc [Re: aerow.thefox]
    #22256246 - 09/18/15 01:31 PM (8 years, 4 months ago)

I try not to touch my jar during transfer, especially dealing with water. I do what you said except take the ring off and slide the lid about halfway off.

I grab the wedge very loosely and it just slides off the scalpel when I hold it almost vertically over. I make sure the arm of the scalpel and my hand don't hover over the LC water.


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Invisibletahoe
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Re: Agar to Lc [Re: taGyo]
    #22256289 - 09/18/15 01:42 PM (8 years, 4 months ago)

In a still air box, etc. I cut the surface of the agar up. Just tear up the surface of a 1/4th inch square area or bigger if you want. Then scrape up the chunks of mycelium, and drop that into the lc. That way you are working with dozens of little shreds of mycelium versus a chunk. It will be usable in a few days doing it that way.


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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Offlineaerow.thefox
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Re: Agar to Lc [Re: taGyo]
    #22256393 - 09/18/15 02:08 PM (8 years, 4 months ago)

Quote:

taGyo said:
I do what you said except take the ring off and slide the lid about halfway off.




i kinda skipped over the part where you take the ring off but leave the lid on. then prepare your agar cuttin and pop and slide.. my bad lol :tongue:


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Edited by aerow.thefox (09/18/15 02:12 PM)


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OfflinePsilosoulful

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Re: Agar to Lc [Re: aerow.thefox]
    #22256436 - 09/18/15 02:20 PM (8 years, 4 months ago)

Why not just make a liquid innoculant instead? It's easier and faster. Why wait for your LC jar to recolonize, then have to suck up the individual strands of mycelium, that are sometimes hard to break up. Too much work IMO. I like to have LI syringes on hand and ready to go!  :awesomenod:


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Invisibletahoe
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Re: Agar to Lc [Re: Psilosoulful]
    #22256491 - 09/18/15 02:35 PM (8 years, 4 months ago)


We have all had that dream of gallons of viable, clean liquid cultures. It's just not the case. I am never impressed with an lc after a month of using it. They get infected so easily.



Quote:

Psilosoulful said:
Why not just make a liquid innoculant instead? It's easier and faster. :awesomenod:




I agree. I make liquid inoculate basically the same way. But instead of transferring the mound of shredded mycelium to a jar, I suck it into a syringe of sterile water.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinebinko
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Re: Agar to Lc [Re: tahoe]
    #22256506 - 09/18/15 02:39 PM (8 years, 4 months ago)

how do you do that, suck a piece of agar into the syringe?


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OfflinePsilosoulful

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Re: Agar to Lc [Re: binko]
    #22256645 - 09/18/15 03:09 PM (8 years, 4 months ago)

Basically take your fully colonized agar and place it in a jar of sterile water, blend, and suck up into your syringe. Here's the tek http://www.shroomery.org/forums/showflat.php/Number/20429745 :super:


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Offlinebinko
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Re: Agar to Lc [Re: Psilosoulful]
    #22256689 - 09/18/15 03:21 PM (8 years, 4 months ago)

isent there any way that dosent require a blender, im abit tight on founds right now


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OfflinetaGyo
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Re: Agar to Lc [Re: binko]
    #22256694 - 09/18/15 03:22 PM (8 years, 4 months ago)

Yeah, just drop the agar wedge into the LC.


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InvisibleMunchauzen
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Re: Agar to Lc [Re: binko]
    #22256873 - 09/18/15 04:08 PM (8 years, 4 months ago)

if you are using
Quote:

binko said:
isent there any way that dosent require a blender, im abit tight on founds right now




check the videos in my signature for a no blender LI tek

im gonna remake the video soon, but you can also scrape the mycelium off the plate with a sterile syringe (inside a sab of course) if the power wash method in my vid doesn't work (it should).


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Offlinebinko
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Re: Agar to Lc [Re: Munchauzen]
    #22256990 - 09/18/15 04:39 PM (8 years, 4 months ago)

i  took a look, and it seems good. Is it ready to use immediately? or do it need to incubate


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Invisibletahoe
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Re: Agar to Lc [Re: binko] * 1
    #22258709 - 09/19/15 12:25 AM (8 years, 4 months ago)

I kind of explain how to do it in one of the post above. Instead of cutting an agar wedge. Take your sterile knife, etc, and scratch the shit out of the surface of the agar. Tearing the mycelium into dozens of little shreds. Now either scoop that up and dump it into your lc. Or take your syringe full of sterile water and squirt a few drops of water onto this pile of shredded mycelium. Then suck the wad back up. No need to cut a wedge and put it in a blender. Way too much work.
Now you have a sterile syringe of liquid inoculate. Use a big syringe if you can. It's the same amount of work to make a 10cc syringe as it is to make a 100cc syringe. There will be more than enough mycelium floating around.

Putting a pile of shredded mycelium into an lc is better than a wedge also. Instead of One big chunk of mycelium, you will have hundreds of pieces floating around your lc on day one. You will be able to use the lc on day 3. You could probably use it on day one if you made sure to suck up a  Pieces of mycelium.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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