Home | Community | Message Board


Everything Mushrooms
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, 10cc Syringe, Brown Rice Flour, Laminar Flow Hood, Malt Extract, Pressure Cooker, Wild Bird Seed

Jump to first unread post. Pages: 1
OfflineJetBlackNinja
Pimp-o-licious
Registered: 11/26/03
Posts: 495
Loc: $%^&
Last seen: 12 years, 1 month
Liquid Innoc. (again)
    #2225365 - 01/07/04 10:17 AM (12 years, 10 months ago)

Well, it's been talked about at least 3 times here this week, and I did a comprehensive search, yet I am still not comfortable going forth with my first liquid Innoc. tonight.

Primarily, what is the best liquid innoculate for:

1) potency

2) Ease of use

What is the best Tek, and what are the best products (brand) to use?

I intend to make enough liquid innoculate to do 6 QT WBS jars, and 25 1/2 pint, MMGG jars, I always use ground Millet instead of BRF in these.


I really welcome any and all input on this, cause I want to start doing this process correctly, right from the outset.

My thanks to the many Shroomerites who have answered these questions for me in the past, and continue to do so to this day.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2225377 - 01/07/04 10:25 AM (12 years, 10 months ago)

PDY is the best, IMO, but karo will work fine and it's easier to use. Get some septum plugs to put in the lids of jars or something unless you're going to be working in a glovebox. Cracking open the lid will probably result in contamination -- if you absolutely must just crack it a bit in back of a flame.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJetBlackNinja
Pimp-o-licious
Registered: 11/26/03
Posts: 495
Loc: $%^&
Last seen: 12 years, 1 month
Re: Liquid Innoc. (again) [Re: micro]
    #2225391 - 01/07/04 10:36 AM (12 years, 10 months ago)

Coolness, just straight 100% Karo syrup right out of the bottle?

And I was going to use a 1/2 pint jar with a small filter, and a self-healing silicone spot for the injection point. This should keep me from having to expose the contents to contams.

Would anyone care to link me to their favorite Teks, also?


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMax1
MaxiMillion

Registered: 01/06/04
Posts: 154
Last seen: 1 year, 11 months
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2225427 - 01/07/04 10:57 AM (12 years, 10 months ago)

Hi JetBlack,
No not straight 100% karo...
Here is the formula.
75-100 cc's of sterile water. (I use 75)
1 tsp of karo or honey.
1/2 pint jar is fine.
PC for 20 minutes, no more than that!
And thats it..I close it up tight rubber seal down. let cool then take to clovebox for inoculating. Ive never had contams.

Karo is crystal clear and easy to see into. (checking growth and contams)
Honey is cloudy and not easy to see into but twice as fast as karo imho.
Do not let it grow out very far, You will be tempted to let it get thick as you can but it will be a big mistake when you try to suck it back up into the syringe.
a couple pieces of broken glass in the jar before you start will help agitate it and break it up in pieces.

Max


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2225439 - 01/07/04 11:03 AM (12 years, 10 months ago)

Right! Glass works wonders, especially if the mycelium is really thick. Only....

Quote:

And I was going to use a 1/2 pint jar with a small filter, and a self-healing silicone spot for the injection point. This should keep me from having to expose the contents to contams.




Watch out -- you want to shake the jars like once or twice a day for airation, so a filter which is porous will leak the liquid. If you only fill the jar like 25% of the way, though, it should have all the air it needs -- just seal and shake every day! Airtight is the best to prevent contamination, anyway -- like I said, I work without a glovebox, so it could be a problem.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJetBlackNinja
Pimp-o-licious
Registered: 11/26/03
Posts: 495
Loc: $%^&
Last seen: 12 years, 1 month
Re: Liquid Innoc. (again) [Re: micro]
    #2225462 - 01/07/04 11:14 AM (12 years, 10 months ago)

okay, what about a larger container?

can I keep it in my incubator at 82-86F?

I need to shake it twice a day? See the funny thing is, afof is really doing these, and he can't be trusted to remember anything.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2225480 - 01/07/04 11:21 AM (12 years, 10 months ago)

Size doesn't matter :wink:

No, really -- any size container will work, but only fill it max 25%.  I use 500 mL bottles and run like 200 mL in each (I guess this is a bit over 25% but whatever.)  200mL = way too much for me, anyway -- that's 20 10cc syringes.  x 5 bottles = 100 10cc syringes....

I would definately shake them every day, though -- you want O2 to dissolve into the liquid.

Also -- if you're using air-tight containers you might want to let them cool inside the P.C. before opening and sealing them -- I didn't let them cool and it formed a vacuum inside the jars.

Temp isn't critical, but 80-85 is the best -- I'm sure 70 would work too but slower.

Good luck!

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinetuco_ramirez
Fistful ofShrooms

Registered: 11/06/03
Posts: 398
Loc: DETROIT!
Last seen: 10 years, 11 months
Re: Liquid Innoc. (again) [Re: micro]
    #2225515 - 01/07/04 11:35 AM (12 years, 10 months ago)

Nice thread, good tips.

I've tried liquid innoc with malt extract, the kind used for beer brewing. However, this tek is, IMHO, very shitty. I've only had one jar out of 4 come out with growth, and it was hit with contams right after I drew the first few syringes. And it was really cloudy so hard to tell what was going on.

I'm going to try the karo tek tonite. Sounds a bit more promising.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJetBlackNinja
Pimp-o-licious
Registered: 11/26/03
Posts: 495
Loc: $%^&
Last seen: 12 years, 1 month
Re: Liquid Innoc. (again) [Re: micro]
    #2226108 - 01/07/04 04:07 PM (12 years, 10 months ago)

Quote:

micro said:
Size doesn't matter :wink:

No, really -- any size container will work, but only fill it max 25%.  I use 500 mL bottles and run like 200 mL in each (I guess this is a bit over 25% but whatever.)  200mL = way too much for me, anyway -- that's 20 10cc syringes.  x 5 bottles = 100 10cc syringes....

I would definately shake them every day, though -- you want O2 to dissolve into the liquid.

Also -- if you're using air-tight containers you might want to let them cool inside the P.C. before opening and sealing them -- I didn't let them cool and it formed a vacuum inside the jars.

Temp isn't critical, but 80-85 is the best -- I'm sure 70 would work too but slower.


--
Micro




Sweet ass. I will keep it in the incubator, that's what it's for anyway.

I will use a QT jar 1/3 full. It will have a silicone self-seal injection point, and a filter to allow for air-exchange.

Do I have to shake, shake? Or can I swirl it around real fast, which would keep the filter dry?


....and to get them started I just empty the contents of a retail spore syringe right into it?


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinetuco_ramirez
Fistful ofShrooms

Registered: 11/06/03
Posts: 398
Loc: DETROIT!
Last seen: 10 years, 11 months
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2226214 - 01/07/04 04:42 PM (12 years, 10 months ago)

What do you use for a filter? There seems to be a problem with my filters getting wet.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJetBlackNinja
Pimp-o-licious
Registered: 11/26/03
Posts: 495
Loc: $%^&
Last seen: 12 years, 1 month
Re: Liquid Innoc. (again) [Re: tuco_ramirez]
    #2226258 - 01/07/04 04:52 PM (12 years, 10 months ago)

Quote:

tuco_ramirez said:
What do you use for a filter? There seems to be a problem with my filters getting wet.




I use a material kinda like poly-fill, but it comes it roles, and is easier to cut into flat even squares.

That is why I would prefer to swirl than shake.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2226261 - 01/07/04 04:55 PM (12 years, 10 months ago)

I'm sure swirling it will do something -- but you really don't even need the filter.

It's up to you....

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMax1
MaxiMillion

Registered: 01/06/04
Posts: 154
Last seen: 1 year, 11 months
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2226271 - 01/07/04 04:58 PM (12 years, 10 months ago)

Quote:
....and to get them started I just empty the contents of a retail spore syringe right into it?


Naaa you dont need that much. 1cc is plenty but 2 will do better.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJetBlackNinja
Pimp-o-licious
Registered: 11/26/03
Posts: 495
Loc: $%^&
Last seen: 12 years, 1 month
Re: Liquid Innoc. (again) [Re: micro]
    #2227709 - 01/08/04 10:14 AM (12 years, 10 months ago)

Quote:

micro said:
I'm sure swirling it will do something -- but you really don't even need the filter.

It's up to you....

--
Micro




Without a filter, how would I get air exchange, and how would I be 100% sure to prevent the vacum effect?

and 2CC, are you sure that's enough for 1/3 a QT of solution?


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2227725 - 01/08/04 10:25 AM (12 years, 10 months ago)

There should be plenty of air in the jar, as long as you fill it 25% of the way, or less, and shake once or twice (better) every day. The air will dissolve into the media.

I don't think you have to worry too much about a vacuum seal -- I was using special narrow-neck jars, but to be sure just let them cool INSIDE THE PRESSURE COOKER and then open it and tighten the lids after everything has cooled. That should do it, I would think.

2cc's should be plenty.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJetBlackNinja
Pimp-o-licious
Registered: 11/26/03
Posts: 495
Loc: $%^&
Last seen: 12 years, 1 month
Re: Liquid Innoc. (again) [Re: micro]
    #2227735 - 01/08/04 10:30 AM (12 years, 10 months ago)

Quote:

micro said:
There should be plenty of air in the jar, as long as you fill it 25% of the way, or less, and shake once or twice (better) every day. The air will dissolve into the media.

I don't think you have to worry too much about a vacuum seal -- I was using special narrow-neck jars, but to be sure just let them cool INSIDE THE PRESSURE COOKER and then open it and tighten the lids after everything has cooled. That should do it, I would think.

2cc's should be plenty.

--
Micro




Thank you kind sir. I will use, no more than 2CC's which is actually good news to these ears.

I still think I may put a small air filter on it, just because I have a knack for growing mycelium VERY slowly.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesalazare
fan_of_shrooms
Registered: 04/29/03
Posts: 185
Last seen: 10 years, 6 months
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2383892 - 02/27/04 05:47 PM (12 years, 9 months ago)

hi, I bought some kind of medicinal band aid breathable tape and i intendd to use this fo a kind of filter, would it work?
thanks.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJetBlackNinja
Pimp-o-licious
Registered: 11/26/03
Posts: 495
Loc: $%^&
Last seen: 12 years, 1 month
Re: Liquid Innoc. (again) [Re: salazare]
    #2384445 - 02/27/04 07:46 PM (12 years, 9 months ago)

For liquid Innoc, I don't use a filter. Just 1 small hole in the lid that has silicone seal on it, for a self-healing injection point.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblesimplemachine
Manfly
Male User Gallery

Registered: 09/14/03
Posts: 1,981
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2384855 - 02/27/04 10:18 PM (12 years, 9 months ago)

Your getting the idea jet! no filters at all. If you have gb or a flow hood, you can use a lid with no holes at all. just as long as you can crack it to shoot the spores in an aseptic enviroment it should be fine. Im a big fan of adding several cc's of peroxide to liquid jars after the spores have sprouted. It seems to speed up the growth and keep things nice and clean.


SM


--------------------
     


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleem_bre_O
shroomery'sembryoticasshole
 User Gallery

Registered: 05/27/02
Posts: 2,278
Loc: In the stages of develope...
Re: Liquid Innoc. (again) [Re: JetBlackNinja]
    #2385646 - 02/28/04 02:34 AM (12 years, 9 months ago)

captain quote.....................


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, 10cc Syringe, Brown Rice Flour, Laminar Flow Hood, Malt Extract, Pressure Cooker, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* new liquid innoc all look contamed will they work? blackout 551 5 01/31/03 07:05 AM
by debianlinux
* vacuum cleaner filter bags for growing? chekck this out! calavera 942 8 06/12/05 11:40 AM
by deanofmean
* liquid innoc solution? beejay 391 5 10/19/04 03:35 PM
by Sammy
* Liquid Innoc Question Alger 499 5 04/01/03 02:14 PM
by diacamomo
* contams at 99.97% effecient filter? need posi pressure glovebox expert... ravin0ff 1,336 9 01/15/05 02:21 PM
by Flashman
* British Triple Filtered Self Healing Liquid Culture Jars - Top Notch! Now with pictures! Apple core 2,004 12 05/10/09 06:24 AM
by Apple core
* seeds, moist filters, cookers .......... keyeghost 524 2 10/07/02 06:50 PM
by keyeghost
* storage of liquid cultures / mycelium syringes in fridge
( 1 2 all )
ohmatic 7,621 20 04/25/05 02:21 PM
by cubicidal

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
816 topic views. 19 members, 90 guests and 22 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Phytoextractum
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.067 seconds spending 0.003 seconds on 14 queries.