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the.dude.abides


Registered: 04/05/15
Posts: 4
Loc: SFBay
Last seen: 3 years, 9 months
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#22825026 - 01/25/16 09:37 PM (8 years, 5 days ago) |
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Got my mini rounds in the cool down process as I type this. PC'ed a bunch of pasty's agar too. Lets get this genetic party started!
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insanemike
Registered: 02/23/14
Posts: 4,272
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Here is my take on it. I cut my colonized cornmeal cakes into wedges and dropped them into sterile water and let them soak for 24 hours. I then noc'd up twelve jars with them and this them at 7 days old.

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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Plate Liquid Inoculation [Re: insanemike]
#22825101 - 01/25/16 09:55 PM (8 years, 5 days ago) |
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ah never thought about doing it that way. its likely the water had some recovery in it. how long did it take to see leap off?
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insanemike
Registered: 02/23/14
Posts: 4,272
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive] 1
#22825241 - 01/25/16 10:30 PM (8 years, 5 days ago) |
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I started to see recovery from the loose cornmeal granulars within 24 hours. Leap off from those granulars took 36-48 hours. It wasn't until 48-60 hours that I began to see recovery from the liquid part of the inoculant and that's when it really began to take off.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Plate Liquid Inoculation [Re: insanemike]
#22828465 - 01/26/16 09:06 PM (8 years, 4 days ago) |
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day 17 untreated coir. just added water, mixed and spawned. grain prepped using cornmeal plate liquid inoculation. riding strong with this clone.

https://www.shroomery.org/forums/showflat.php?Cat=0&Number=22828419&page=0&vc=1#22828419
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insanemike
Registered: 02/23/14
Posts: 4,272
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#22828782 - 01/26/16 10:25 PM (8 years, 4 days ago) |
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Quote:
eatyualive said: day 17 untreated coir. just added water, mixed and spawned. grain prepped using cornmeal plate liquid inoculation. riding strong with this clone.

https://www.shroomery.org/forums/showflat.php?Cat=0&Number=22828419&page=0&vc=1#22828419
That looks great, eat.
check this out
Pioppini

I also found some king oyster pins on a cornmeal cake plate. I've never had invitro pins of anything but cubes when using agar. I haven't used agai in about 2 months.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Plate Liquid Inoculation [Re: insanemike]
#22828807 - 01/26/16 10:34 PM (8 years, 4 days ago) |
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#22830183 - 01/27/16 11:24 AM (8 years, 4 days ago) |
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the cornmeal is killin it! Wooo Hooo!
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Plate Liquid Inoculation [Re: PsiloPsychIn]
#22831322 - 01/27/16 05:18 PM (8 years, 4 days ago) |
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its extremely clean. ive g2ged grain prepped by cornmeal more than 2 months now. and i haven't had one tub contaminate before a 3rd flush.
pretty much the entire official aa plus thread is all cornmeal plate liquid inoculation.
i had one round of the brown rice flour plate liquid inoculation but it was a little slow for my liking.

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the.dude.abides


Registered: 04/05/15
Posts: 4
Loc: SFBay
Last seen: 3 years, 9 months
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#22831555 - 01/27/16 06:23 PM (8 years, 4 days ago) |
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Nice tek! Got my reboy plates colonizing on cornmeal. Now say there are only 10 jars of prepped wbs ready for the cornmeal inoculate... would it be advisable to lower the water to cornmeal ratio so as not to flood the jars, or just mix at prescribed dilution rate that has obvious results and dump remaining mixture after.... hmm or try to fill sterile syringes with the remainder and refrigerate. Any input or ideas would be awesome.
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
Last seen: 2 days, 23 hours
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The Dude... I've been thinking for some time about letting the cornmeal water mix sit for a spell before using. The myc tends to stay on top of the cornmeal ... Meaning when you dump it into water there is going to be s fair bit of un utilized nutes - after a few days you'd have more of an LC....
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Plate Liquid Inoculation [Re: PsiloPsychIn]
#22832126 - 01/27/16 09:12 PM (8 years, 3 days ago) |
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you don't want to go too low on the water ratio or it gets a little hard to pour. you could lower it a bit. but i would just do the normal 1-3cc volume of liquid inoculant. and just pour the leftover in the last jar and see how it does. generally i like to have more than enough jars so it does not go to waste. there have been times when i don't have enough LI for all the jars i have. but id rather have that, than too few jars. now, reason i say pour it in the last jar is that if you do pour too much. you only fuck up one jar. ideally you don't want to fuck up any jars. 9-10 times it just colonizes faster than all the other jars. but there is a higher risk when you add too much water. i would in your case, pour a small percentage more per jar to equal out the entire LI volume. or just pour the leftover in the last jar.
if you don't have the time to spare that one jar. just toss out the extra that is leftover. remember, your only using 1/4 pint of water to a full plate of mycelia. so if your adding 1/8th of water. it will be a muddier mix. try it see how it works out. i wouldn't go lower than that volume though as it may not blend properly.
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the.dude.abides


Registered: 04/05/15
Posts: 4
Loc: SFBay
Last seen: 3 years, 9 months
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#22832390 - 01/27/16 10:25 PM (8 years, 3 days ago) |
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Quote:
PsiloPsychIn said: The Dude... I've been thinking for some time about letting the cornmeal water mix sit for a spell before using. The myc tends to stay on top of the cornmeal ... Meaning when you dump it into water there is going to be s fair bit of un utilized nutes - after a few days you'd have more of an LC....
Good point Psilo... this can be worth exploring if I had the resources. Perhaps a future endeavour. Right now I need hella myc tearing through the wbs
Quote:
eatyualive said: you don't want to go too low on the water ratio or it gets a little hard to pour. you could lower it a bit. but i would just do the normal 1-3cc volume of liquid inoculant. and just pour the leftover in the last jar and see how it does. generally i like to have more than enough jars so it does not go to waste. there have been times when i don't have enough LI for all the jars i have. but id rather have that, than too few jars. now, reason i say pour it in the last jar is that if you do pour too much. you only fuck up one jar. ideally you don't want to fuck up any jars. 9-10 times it just colonizes faster than all the other jars. but there is a higher risk when you add too much water. i would in your case, pour a small percentage more per jar to equal out the entire LI volume. or just pour the leftover in the last jar.
if you don't have the time to spare that one jar. just toss out the extra that is leftover. remember, your only using 1/4 pint of water to a full plate of mycelia. so if your adding 1/8th of water. it will be a muddier mix. try it see how it works out. i wouldn't go lower than that volume though as it may not blend properly.
Thanks Eat... I figure with 4oz water the math ends up being around 9.8ml per jar if I do 12 jars.... that's not unheard of in the realm of agar Liquid Inoculation if I am reading correctly I see Muda uses 10cc per Quart. And yes, ideally I'd rather not fuck up anything. That being said, maybe I use 3oz water to noc each jar with 7ml cornmeal LI.
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the_r3dz
Trich Propagator


Registered: 03/26/15
Posts: 5,709
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So I did this for the first time today. No blender so I'm just going to throw it into a jar of sterile water with a piece of glass in it and shake the shit out of it we'll see what happens.
However, once I pulled my plates out of the PC the color had all gone from the once blue cornmeal. I used Bob's organic or whatever, PC'd at 17psi for about 45 minutes maybe a little more.
Where did my color go?
Edited by the_r3dz (01/28/16 12:36 AM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Plate Liquid Inoculation [Re: the_r3dz]
#22833022 - 01/28/16 05:51 AM (8 years, 3 days ago) |
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that will be much more difficult than you think. the cornmeal forms sort of a mat of mycelia. id wait until you get a blender.
did the color turn back to cornmeal color? strange.
what color did you add?
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the_r3dz
Trich Propagator


Registered: 03/26/15
Posts: 5,709
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#22833810 - 01/28/16 10:55 AM (8 years, 3 days ago) |
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Oh. Well...I did it with an mea chunk once, the chunk hardly broke up but I still managed to get some mycelia off, maybe I just need to use a really soft agar mix for what I'm going for but whatever I'm still going to see how this goes. Maybe if I use a softer cornmeal mix? Slightly more water, cooked down slightly less. I've already spent so many hundreds of dollars on this hobby, and since I'm not making any of it back I'm hesitant to spend more on yet another machine. The AA930 ruined my budget.
I didn't add coloring, I used blue cornmeal from Bob's mill. It's like pinkish tan now. Maybe I'll take pics
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Plate Liquid Inoculation [Re: the_r3dz]
#22835899 - 01/28/16 08:19 PM (8 years, 2 days ago) |
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well agitating it will work. you will probably be able to inoculate jars and have good growth. but it won't be nearly as fast as if you blended it up evenly. you can get blade assembly sets at walmart for 10$. it comes with a blade, a black blender base, 2 gaskets.
oster brand blender is what you want. and you could simply use a power drill instead of the blender if you don't have a blender.
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#22835951 - 01/28/16 08:29 PM (8 years, 2 days ago) |
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Hey Eat, the cornmeal and rice flour plates can full colonize? Or just the top like agar.
Edited by Mr. Alien (01/28/16 08:49 PM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#22835983 - 01/28/16 08:34 PM (8 years, 2 days ago) |
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I have a quick question. I like the idea of cloning pins grown on agar, do pins grow better on cornmeal/brf plates than on agar? It makes for a hell of a LI but maybe I could use this tek for this as well!
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#22835985 - 01/28/16 08:34 PM (8 years, 2 days ago) |
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Quote:
eatyualive said:
Th My theory is that the thicker viscosity of the slurry and cornmeal blends allow the inoculant to stick or cling to the grain better. Where you may have a regular agar plate blend slip down the side of the grain. ยก
I've been thinking about that since months. I always wanted A Liquid inoculant viscous enough to do that.
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