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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: keifnnugs]
    #22606421 - 12/02/15 09:23 PM (8 years, 1 month ago)

I have done spores to cornmeal and transferred that myc to more cornmeal.

It is slightly harder to detect bacteria on cornmeal but even with agar there is always a chance you missed something with a transfer...

So go for it!
Use good sterile procedure and report back :smile:


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22606435 - 12/02/15 09:26 PM (8 years, 1 month ago)

i think i had some bacteria and it appeared milky white on the cornmeal plate. it was very discernible from a clean plate. :thumbup:

here is the image you can see the milky discoloration.



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Invisibleinsanemike
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22606467 - 12/02/15 09:34 PM (8 years, 1 month ago)

I too have seen bacteria on a few cornmeal plates. I transfered king oyster culture from agar to cornmeal which came from a vendor lc. A few days after the transfer, a few plates had visible bacteria and a couple had green mold. Since then I have not usesd any vendor spores or cultures and have yet to see bacteria or mold on about 4-5 dozen plates that I have made since then.


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OfflineMachiavelliavore
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22606477 - 12/02/15 09:37 PM (8 years, 1 month ago)

I culture on grain petris to some degree.  It doesn't have to be agar, and I can't imagine cornmeal plates being harder than grain petris, though transfering a grain or cutting under the contaminated surface after sanitizing the jar with bleach can be a nice way to get away from some contams.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: insanemike]
    #22606481 - 12/02/15 09:38 PM (8 years, 1 month ago)

Yeah that is consistent with how I've spotted bacteria too...
I find it easy enough to spot the bacteria (and other contams)

It just doesn't stand out as starkly as on an agar plate... especially if you back light it the agar plate...


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Invisibleinsanemike
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22606526 - 12/02/15 09:51 PM (8 years, 1 month ago)

I am kind of on the fence about whether its a positive or a negative with contams and cornmeal mush. What I mean is, culture's seem to grow a little slower on cornmeal but tend to look a lot healthier. One would think that a slower growing culture would have a disadvantage when it comes to out competing a contamination. Then again, can a healthier culture keep contams more at bay?


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OfflineMachiavelliavore
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: insanemike] * 1
    #22606573 - 12/02/15 10:04 PM (8 years, 1 month ago)

A faster culture can smother a contam faster, but maybe some slower cultures smother it better?

Hard to say, some slow cultures are probably slow because they are more thorough or some shit, while others just suck.

I think I'll be trying some PF plates again soon.  I made some and they colonized decently quickly (1/4")

What I found works well is putting a decent agar wedge on, then shaking it all over the surface.  Mycellium really seems to love PF substrate, and the mass of mycellium for LI is excellent.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineMachiavelliavore
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Machiavelliavore]
    #22614889 - 12/04/15 09:13 PM (8 years, 1 month ago)

While the jars I made with cornmeal LI took like 5-6 days to get rolling, the inoculation power and coverage looks EXCELLENT.  No shaking required or missed spots.  Definitely a winner in that regard, onpar with slurry.

I also just knocked up my next cornmeal plate with the cornmeal LI.  Should give me a good idea of how clean it is, and give me a fully colonized plate whenever I want it.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Machiavelliavore] * 1
    #22627408 - 12/07/15 07:49 PM (8 years, 1 month ago)

i blended up my AA+ clone on the brf pasty plate tonight. i liquid inoculated 20 quarts of oat grain. i have to get more oats. went through 200lbs.

so far my observations on the brf plate are as follows.

1) the brf plates take much longer to fully colonize a plate than regular pasty plate formula and cornmeal plate in the op.
2) when i make the mix too pasty or dry, i do have a little difficulty flipping the culture over for the tiger drop.
3) transfers with brf and cornmeal take a little craft. when i cut a wedge. its almost a slice of just mycelia and no plate. the mycelia forms a nice mat on the top of the plate. and doesn't seem to penetrate lower than the surface.

anyway, ill keep trying this out.

round 1 is under way.


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Invisibleinsanemike
Registered: 02/23/14
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22627525 - 12/07/15 08:12 PM (8 years, 1 month ago)

Quote:

eatyualive said:
i blended up my AA+ clone on the brf pasty plate tonight. i liquid inoculated 20 quarts of oat grain. i have to get more oats. went through 200lbs.

so far my observations on the brf plate are as follows.

1) the brf plates take much longer to fully colonize a plate than regular pasty plate formula and cornmeal plate in the op.
2) when i make the mix too pasty or dry, i do have a little difficulty flipping the culture over for the tiger drop.
3) transfers with brf and cornmeal take a little craft. when i cut a wedge. its almost a slice of just mycelia and no plate. the mycelia forms a nice mat on the top of the plate. and doesn't seem to penetrate lower than the surface.

anyway, ill keep trying this out.

round 1 is under way.





I too have experienced the thick mat of mycelium on top of the cornmeal mush. For this reason, I was thinking of spooning about half as much cornmeal mush into each pasty as I have been. I have been cutting each playe into 12 wedges and dropping 3-4 wedges into each grain jar. I have had mixed results with the distribution of culture throughout the grain jars, no matter how intently I shake them. I was thinking of purchasing one of those blender bottles with the metal mesh balls in them. Have you tried those yet?


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OfflineMachiavelliavore
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22627895 - 12/07/15 09:34 PM (8 years, 1 month ago)

I think a sterilized fork is pretty nice for making the transfers.  Since I don't use minirunds, I just scooped under the mycellium on the corneal, got about the right size thing, stabbed it, and dumped it.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflinetaGyo
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Machiavelliavore]
    #22629223 - 12/08/15 09:28 AM (8 years, 1 month ago)

You rock Eat!



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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: insanemike]
    #22635206 - 12/09/15 06:05 PM (8 years, 1 month ago)

Quote:

insanemike said:
Quote:

eatyualive said:
i blended up my AA+ clone on the brf pasty plate tonight. i liquid inoculated 20 quarts of oat grain. i have to get more oats. went through 200lbs.

so far my observations on the brf plate are as follows.

1) the brf plates take much longer to fully colonize a plate than regular pasty plate formula and cornmeal plate in the op.
2) when i make the mix too pasty or dry, i do have a little difficulty flipping the culture over for the tiger drop.
3) transfers with brf and cornmeal take a little craft. when i cut a wedge. its almost a slice of just mycelia and no plate. the mycelia forms a nice mat on the top of the plate. and doesn't seem to penetrate lower than the surface.

anyway, ill keep trying this out.

round 1 is under way.





I too have experienced the thick mat of mycelium on top of the cornmeal mush. For this reason, I was thinking of spooning about half as much cornmeal mush into each pasty as I have been. I have been cutting each playe into 12 wedges and dropping 3-4 wedges into each grain jar. I have had mixed results with the distribution of culture throughout the grain jars, no matter how intently I shake them. I was thinking of purchasing one of those blender bottles with the metal mesh balls in them. Have you tried those yet?



thats a great idea actually. i believe my cornmeal plates are usually about double the height of the agar plates. so it could definitely be recduced.
yes i have. check it out.

dhearic made one, then i made one also. dhearics is at the bottom of the op. mine is right after that.

http://www.shroomery.org/forums/showflat.php/Number/21927954#21927954

here is the one i made. make sure to heavily score the plastic surface of the bottle so that when you silicone the seal catches on the plastic. that is where the leaks happen.

http://www.shroomery.org/forums/showflat.php/Number/21927992#21927992


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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22635391 - 12/09/15 06:42 PM (8 years, 1 month ago)

Eatyualive, can the top part (with the pour spout) of the blender bottle you customized be run through a PC cycle?

Is it PP 5 also?


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: taGyo]
    #22635408 - 12/09/15 06:44 PM (8 years, 1 month ago)

Quote:

taGyo said:
You rock Eat!





what did i do? or did i do that? lol..


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22635411 - 12/09/15 06:45 PM (8 years, 1 month ago)

Quote:

PsiloPsychIn said:
Eatyualive, can the top part (with the pour spout) of the blender bottle you customized be run through a PC cycle?

Is it PP 5 also?



yes. but it is not pp5. actually if you look in the tribal container thread. we were talking about it here.

http://www.shroomery.org/forums/showflat.php/Number/22526020#22526020


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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22635435 - 12/09/15 06:50 PM (8 years, 1 month ago)

Thanks Eat, I'm embarrassed now... I should have done my homework. :shrug:


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22635448 - 12/09/15 06:51 PM (8 years, 1 month ago)

nah man no problem. easier for me to link that then retype because i had the thread open.:thumbup:


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive] * 1
    #22649370 - 12/13/15 09:40 AM (8 years, 1 month ago)

initial trials with the brown rice flour liquid inoculation are not very impressive. with the slow growth in the plate and regular speed for the LI. It isn't making much sense to continue. Although I will try it a few more times before ruling the brf out.

this is day 5 on the jars i inoculated. im goiing to shake them today. my goal is to have the jars fully colonized without a shake on day 5. so far, cornmeal rules.



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InvisiblePastywhyteMDiscord
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22649398 - 12/13/15 09:51 AM (8 years, 1 month ago)

Bummer eats. Looks like I may need to pick up some cornmeal then.


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