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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22437759 - 10/26/15 09:15 PM (8 years, 3 months ago)

Some Cornmeal Plate Liquid Inoculation AA+ tubs coming along nicely! This is with polyfil stuffed in the entire process. so there are two experiments going on at once.(as usual, it calms the mind)

Tub 1


Tub 2


Tub 3


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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22439527 - 10/27/15 10:07 AM (8 years, 3 months ago)

Quote:

eatyualive said:
Thanks paper back. I'll give this a go next. Sounds exactly like my wbs prep. Only time I get clumping is when I don't let the grain strain out for 10 mins. When I load it wet, I got clumping once. That was me In a hurry. Only happened on my last batch.




I forgot to mention I put the lid on during the soak to keep the heat in.

Let me know how it goes :smile:  I'm sure you have a better idea of what proper hydration is then me so I'm curious how it stacks up against other methods you've tried.

*edit*  And those tubs are looking nice man!  My straight into fruiting tub is knotting a bit but doesn't seem too far ahead of my other tubs.  We'll see how it goes.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley


Edited by paperbackwriter (10/27/15 10:21 AM)


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OfflinetaGyo
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: paperbackwriter]
    #22439550 - 10/27/15 10:12 AM (8 years, 3 months ago)

Wait so you never used tape on those tubs Eatyu?


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: taGyo]
    #22441553 - 10/27/15 06:56 PM (8 years, 3 months ago)

Quote:

taGyo said:
Wait so you never used tape on those tubs Eatyu?



nope. never have in all my time of growing until 2013 when i was putting some methods to the test. i mean the methods worked. so no knock on the methods. my thing is, if i put tape on. take it off, then put poly in. im doing 3 things. if i put the poly in i do 1 thing. its less work.

if you do one tub its irrelevant. if you do multiple tubs, it makes a big difference in time management.


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: paperbackwriter]
    #22441561 - 10/27/15 06:57 PM (8 years, 3 months ago)

Quote:

paperbackwriter said:
Quote:

eatyualive said:
Thanks paper back. I'll give this a go next. Sounds exactly like my wbs prep. Only time I get clumping is when I don't let the grain strain out for 10 mins. When I load it wet, I got clumping once. That was me In a hurry. Only happened on my last batch.




I forgot to mention I put the lid on during the soak to keep the heat in.

Let me know how it goes :smile:  I'm sure you have a better idea of what proper hydration is then me so I'm curious how it stacks up against other methods you've tried.

*edit*  And those tubs are looking nice man!  My straight into fruiting tub is knotting a bit but doesn't seem too far ahead of my other tubs.  We'll see how it goes.



yep as do i. ill do it just like you next time. always trying to refine technique to find the absolute minimums and easiest ways.
:chief:


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22441982 - 10/27/15 08:34 PM (8 years, 3 months ago)

update
Quote:

eatyualive said:
Some Cornmeal Plate Liquid Inoculation AA+ tubs coming along nicely! This is with polyfil stuffed in the entire process. so there are two experiments going on at once.(as usual, it calms the mind)

Tub 1


Tub 2


Tub 3





the light source is kind of to the side of the tubs. you can see the fruits stretching towards it sideways.


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22445553 - 10/28/15 03:59 PM (8 years, 3 months ago)

cornmeal plate LI for the win! 2nd to none hands down. slurry comes in 2nd now only because you have to make pf jars additionally. but if your doing 3 transfers on agar its a moot point. slurry is faster by one day. this is very comparable.




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OfflineMachiavelliavore
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22450298 - 10/29/15 05:56 PM (8 years, 3 months ago)

Do you think cornmeal plates can be steam sterilized in a reasonable amount of time?  You probably didn't determine that sterilization time of 45mins, and just did what you estimated to be safe for the amount of substrate.  It seems like this might be a better way to make LI for people wanting to up their PF tek game without a PC.

If the blender blade was submerged in boiling water for the pot sterilization, ie jar laying on its side, do you think that would get it clean enough for LI?

I think I'll definitely try to develop this as a no-PC tek.  Texturally they look a lot better than BRF agar substitute.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Machiavelliavore]
    #22450454 - 10/29/15 06:38 PM (8 years, 3 months ago)

Only way to find out is to try it. Once you pull the blender out of the water it is contaminated.


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OfflineMachiavelliavore
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22450695 - 10/29/15 07:34 PM (8 years, 3 months ago)

I'm not sure if a fully sealed jar would crack steam sterilizing.

I have heard of problems with non-pc'd blender blades, so my thought was make sure the heat transfer was excelent.  If the jar does crack, I could see leaving the band ever so slightly loose, grabbing it with tongs at the end of the steam sterilization, then twisting the band fully shut.  Another jar could be sterilized normally to get the appropriate amount of water and the blade could be easily switched to that jar.

Perhaps even sterilzing the blade upside down in an empty jar would be okay.  For air lets see, 300K-->373K  P0=1atm  P1/P0=373/300  ~ P1=1.25atm=3.67 psi in the jar if it doesn't expell gas through the seal.  I think that'd probably crack it.  Medium tight might minimize the water coming in while allowing expanding gas out.  Half Pint  H=3.5"  D=2.5"  2pi(1.25")^2 + pi*1.25"*3.5" = 9.82 + 13.75 = 23.57""*3.67lbs/in^2
= 86 lbs internal force


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Machiavelliavore]
    #22451436 - 10/29/15 09:47 PM (8 years, 3 months ago)

well light source is adjusted. anyways. looks like the cornmeal LI worked.






Dung added to the sub FTW!


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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22454278 - 10/30/15 04:27 PM (8 years, 3 months ago)

You guys never fail to impress me! 1-2 more transfers and off to cornmeal plates I go then into my new Tribal blender  :hellyeah:


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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: magicMerlin]
    #22494773 - 11/08/15 08:57 AM (8 years, 2 months ago)

King oyster on cornmeal mush. I'm going to drop some into a couple of 1/4 pint jars of sterile water and let it dissolve until I get back from my camping trip.



I'm going to put some tissue from this AA+ clone on some cornmeal mush next.



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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: insanemike]
    #22495075 - 11/08/15 10:36 AM (8 years, 2 months ago)

This is AWESOME Mike!
First time I've seen an edible on the cornmeal mush! Seems plausible that anything that grows on agar will also grow on this but you have tested that assumption.
Thank you!

Sweet clone by the way... love that fat stipe ... is it pretty solid?


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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22495214 - 11/08/15 11:11 AM (8 years, 2 months ago)

Quote:

PsiloPsychIn said:
This is AWESOME Mike!
First time I've seen an edible on the cornmeal mush! Seems plausible that anything that grows on agar will also grow on this but you have tested that assumption.
Thank you!

Sweet clone by the way... love that fat stipe ... is it pretty solid?




I wasn't sure how well the king oyster myc would grow on it. It seemed to start off a bit slow but really took off a few days after recovery.TheAA+ clone stipe is dense but not completely solid. It was the most dense, tallest fruit from the firsr 2 tubs that I grew out of them. The original clone came from an agar pin. Hopefully this clone of a clone gives me a better yield than those last 2 tubs.


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OfflinePsiloPsychIn
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: insanemike]
    #22495246 - 11/08/15 11:21 AM (8 years, 2 months ago)

My experience with the cubes on the corn meal mush was similar... it seems like there is a few days after transfer or germination where it just sort of sits there ... then takes off.

My assumption is this... at a microscopic level the myc has a somewhat more difficult time getting to the nutrients in the cornmeal mush but once it connects to it is has more nutrients and grows faster.

As for the CMLI I think each little piece of cornmeal is like a nutrient well that the myc can grow out of once transferred to grains or other substrate and that explains the faster jump off and colonization that Eat has observed.


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #22495633 - 11/08/15 01:14 PM (8 years, 2 months ago)

I have a plate of CRS that was swiped with spores that colonized in a few days. i need to transfer it. i made more cornmeal and regular plates. Here is the CRS.



also here is a 2nd flush from the Cornmeal slurry plate tubs. I also harvested 2 other tubs but didn't take pictures.

I re-hydrated for 3rd flush but may toss when the new spawn is ready and refill the tubs.



consensus is that cornmeal plates liquid inoculation is no more contam prone than any other method. ill even dunk a few tubs on the next round to put it to the real test.


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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #22496774 - 11/08/15 05:11 PM (8 years, 2 months ago)

My experience has been that these plates colonize quite slowly.  I think I did a couple on the 28th or 31st and both of them have just barely started to expand past the initial wedge.  IDK my cultures or agar are weird.  Always seems to take 3-4 days to get rolling and grow pretty slowly.  Tend to be pretty tomentose on agar as well.

The cornmeal plates did appear slightly wet/glistening on the surface, so perhaps I didn't cook out enough water on the stove.

All 5 cultures I'm looking at right now grow at almost the same pace.  Testing pastyplate mix side by side with grainwater and there doesn't appear to be any difference.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (11/08/15 05:12 PM)


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Machiavelliavore]
    #22496831 - 11/08/15 05:22 PM (8 years, 2 months ago)

one thing im noticing big time on this cornmeal inoculation. make sure to take the extra time to give the jar a good shake after your inoculation is complete. a full minute on each jar can actually help speed growth. i have been shaking for around 20 seconds each jar. but now ive upped it to about 45 seconds to 1 minute each. i notice when i don't thoroughly shake the jars evenly enough those jars take longer to colonize than the others. sometimes up to a week longer.


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Invisibleeatyualive
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Machiavelliavore]
    #22496841 - 11/08/15 05:23 PM (8 years, 2 months ago)

Quote:

Machiavelliavore said:
My experience has been that these plates colonize quite slowly.  I think I did a couple on the 28th or 31st and both of them have just barely started to expand past the initial wedge.  IDK my cultures or agar are weird.  Always seems to take 3-4 days to get rolling and grow pretty slowly.  Tend to be pretty tomentose on agar as well.

The cornmeal plates did appear slightly wet/glistening on the surface, so perhaps I didn't cook out enough water on the stove.

All 5 cultures I'm looking at right now grow at almost the same pace.  Testing pastyplate mix side by side with grainwater and there doesn't appear to be any difference.



mine looked glossy like that and i fruited nice flushes off of it in the last few posts above. no contams. im on 2nd flushes with those now. i updated the op. look near the end. that shiny look threw me off at first.

they are slower initially. but catch up later.

i had a plate from spores colonize in a few days.


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