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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23101089 - 04/09/16 05:44 PM (7 years, 9 months ago) |
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Even though agar/ cormeal only colonizes on the surface dosen't filaments of myc run down through it? (Why colored agar looses its color even on the bottom & backsides of wedges fuzz up in jars) some extra work but what if you layered cormeal on the bottom of a plate and poured a thin layer of agar over the top? Just an idea not sure if it would make much of a difference.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: tombosley8]
#23101098 - 04/09/16 05:46 PM (7 years, 9 months ago) |
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yes exactly like pastys. the only difference is the cornmeal and the ratio of the mix is a little different. but the plate method is the same. ND, it might. but ive never let a plate go long enough after 98% colonization. im generally blending it up into LI by then.
im not sure about layering. but, my next trial will be mixing both together. some pda and some cornmeal. maybe make the best of both worlds. in the end its always about finding the right ratio. anything and everything can be done if you find the right mix.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23103672 - 04/10/16 02:31 PM (7 years, 9 months ago) |
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Do your cornmeal/brf plates come out pretty soft? Or should they be nearly as hard as agar?
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: tombosley8]
#23104149 - 04/10/16 05:16 PM (7 years, 9 months ago) |
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Eat can answer this also but in my experience they should be nearly as hard as agar.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
#23104867 - 04/10/16 08:48 PM (7 years, 9 months ago) |
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yeah like agar.
if your finding they are too soft. you can always boil the cornmeal/water a little longer before pouring it into the plate. when i pour it, its a pasty consistency.
Edited by eatyualive (04/10/16 08:49 PM)
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thisisfielding
the dude

Registered: 01/30/15
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23114658 - 04/13/16 08:09 PM (7 years, 9 months ago) |
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So I have two plates ready to blend for 25-ish jars. Would it be better to combine them together and blend or do them individually? How easy is it to see contamination on these plates - if by the time I blend its only white mycelium growth should I be good to go?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: thisisfielding]
#23116358 - 04/14/16 09:12 AM (7 years, 9 months ago) |
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i would do each separately. and one plate should be enough for 25 quart jars of grain. how many transfers have you done? if you have done a few and it looks clean then yes it should be fine. and yes you can see contams just like an agar plate. its a little easier if your using the colored cornmeal or a food coloring with your cornmeal if you can't find the blue cornmeal. its not really any different than using regular agar plates. just a little more nutritious and it colonizes a tad bit slower. but the growth is vigorous. i often will transfer from regular agar plates like PDA to cornmeal and see an explosion of robust growth.
use a regular mouth half pint jar. fill it half full with water. pc 20 mins at 15psi. once cool take into your SAB. wipe jars and plate with 70% iso. then loosen both lids. lift both lids simultaneously as you tiger drop your entire plate into the blender jar. put the lid of the blender jar back on. then blend it up on your blender base. wipe with iso again before taking it back into the sab. In a SAB i often do my blend, then clean all the jars and bring them into the SAB once the jar is ready for inoculation. in a shmuv, i clean all the quarts of spawn with iso first then put them in. bring in the blender jar/plate and do my drop. then blend it up on my base. then wipe with iso and bring it back into the shmuv.
either way works depending on what you have to work with.
its also wise to have two blender bases. you can get those at walmart in a repair kit for like 10$. it comes with a blender blade, black base, 2 gaskets. its often hidden on the upper shelf near the oster blenders. check your local walmart stock prior to going there because they generally list it in stock online. it will save you the trip.
Edited by eatyualive (04/14/16 06:34 PM)
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thisisfielding
the dude

Registered: 01/30/15
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23117811 - 04/14/16 05:31 PM (7 years, 9 months ago) |
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Wow, thanks for the thorough response Eat! I've done 3 transfers from agar and then the final to the cornmeal plates - they look great but Im not familiar with using cornmeal so I don't know how easy its to spot contams. Is there a reason why we use them before they hit the sides of the container? I just ask because I have one of my plates that just hit one of the sides, healthy growth none the less. I have way more LI then I need then if 1 plate does 40 jars haha. Do you have a flowhood too? I always feel so paranoid using my flowhood and almost feel safer using a SAB.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: thisisfielding]
#23117880 - 04/14/16 05:50 PM (7 years, 9 months ago) |
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just in case there are any contams on the edge. ive also used plates that are fully colonized with no issues. but you don't want to let it go too far up the side of the plate.
nope i use a shmuvbox or a SAB. depends on how many jars im doing at once. if its under 20 then maybe a SAB. If its more Ill do the shmuvbox because i can fit many more jars in the 55 gallon bag.
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thisisfielding
the dude

Registered: 01/30/15
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23118026 - 04/14/16 06:23 PM (7 years, 9 months ago) |
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So I see you recommend using a pint jar / filling it half full so ~8 oz. I thought you were doing 1/4 a pint per plate in the original tek. I would prefer speed over quantity if it comes down that unless you think that theres no difference between the two.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: thisisfielding]
#23118064 - 04/14/16 06:34 PM (7 years, 9 months ago) |
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sorry i mis typed. regular mouth half pint jar.
yes 1/4 pint. i fixed it.
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thisisfielding
the dude

Registered: 01/30/15
Posts: 178
Last seen: 7 years, 7 months
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23118108 - 04/14/16 06:44 PM (7 years, 9 months ago) |
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Quote:
eatyualive said: sorry i mis typed. regular mouth half pint jar.
yes 1/4 pint. i fixed it.
Perfect! Got it going . I just made some agar plates of the texas orange cap print you sent me, after a few transfers it'll be making it to cornmeal. I'm really interested in finding aggressive growers and I heard the texs are.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: thisisfielding]
#23118660 - 04/14/16 09:00 PM (7 years, 9 months ago) |
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yes they definitely are.
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the_r3dz
Trich Propagator


Registered: 03/26/15
Posts: 5,709
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23118837 - 04/14/16 09:46 PM (7 years, 9 months ago) |
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Wow you use the shmuvbox? I always sorta thought that was kind of a ridiculous thing, don't think I've seen anyone else using it. But knowing you and how many you have out there it's probably your tek :P so many times I'll look back at a tek that I read when I first registered and realized it was yours all along. When did you get your TC tag?
How many jars can you actually fit into the shmuv? I got myself a gigantic (mostly tall) SAB and I love it. I can do huge bags g2g or 24 jars at a time.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: the_r3dz]
#23121293 - 04/15/16 04:04 PM (7 years, 9 months ago) |
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yes i prefer a shmuv over everything else. i can fit 50 quarts in a shmuv so all work can be done in one sitting. i also traded my flowhood in for a shmuvbox. it packs up nice and small into the box. and its inconspicuous. not big and bulky like a flowhood or a 110 quart sab with 5" arm holes cut in it. try hiding a tub that big somewhere conveniently.
i guess i got a tc tag when they invented the tc tag. i logged in one day and had it. but even back then i didn't post much here. i don't recall when that was. been a tc longer than many of the members on this forum have been registered.
i basically had a total post count of 1500 posts in 2013-2014 when i logged back into this forum from a 5 year break.
stealth is key. being able to walk into a grow room and not be able to tell its a grow room is ideal.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23121315 - 04/15/16 04:09 PM (7 years, 9 months ago) |
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Remember that youtube vid you posted some days ago? About those guys working with a shmux? I loved it, it really is an awesome thing. Do you know if I can get that blower (or whatever it's called) off the internet?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Josex]
#23121331 - 04/15/16 04:12 PM (7 years, 9 months ago) |
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its a room size hepa unit. you buy the 99% true type hepa for it. unit cost about 35-40$. you can get all different types. but you want the out take to be on the top of the unit. odd shaped units don't fare to well.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
#23121359 - 04/15/16 04:21 PM (7 years, 9 months ago) |
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Quote:
eatyualive said: its a room size hepa unit. you buy the 99% true type hepa for it. unit cost about 35-40$. you can get all different types. but you want the out take to be on the top of the unit. odd shaped units don't fare to well.
That sounds like Mandarin to me right now , I'll have a gander at that link you sent me, thanks a lot!
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GlazedHazels
Reality Outran Apprehension

Registered: 08/12/11
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Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
#23762882 - 10/23/16 12:13 AM (7 years, 3 months ago) |
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Can the cornmeal replace all need for agar?
How much faster than blended agar is this?
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,068
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Re: Cornmeal Plate Liquid Inoculation [Re: GlazedHazels]
#23763229 - 10/23/16 04:52 AM (7 years, 3 months ago) |
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Quote:
GlazedHazels said: Can the cornmeal replace all need for agar?
How much faster than blended agar is this?
Any medium that can be reasonably colonized and inoculated cleanly and 'cut' into can be used in place of agar.
Look up 'brown rice flour agar'
People have been slurying brown rice flour and water together and putting it in vessels for sterilization for a long time now.
Most don't recommend it. Agar is just so nice and easy to cut into compared to mediums that don't dissolve in water.
It is by no means a 'replacement' for the convenience of agar.
But yeah as long as you don't mind a little extra work when slicing into it for transfers you can use corn meal plates for all your culturing.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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