Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | Next >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineNDStepp84
Stellar nuclear waste
Male User Gallery

Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23101089 - 04/09/16 05:44 PM (7 years, 9 months ago)

Even though agar/ cormeal only colonizes on the surface dosen't  filaments of myc run down through it? (Why colored agar looses its color even on the bottom & backsides of wedges fuzz up in jars) some extra work but what if you layered cormeal on the bottom of a plate and poured a thin layer of agar over the top? Just an idea not sure if it would make much of a difference.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: tombosley8]
    #23101098 - 04/09/16 05:46 PM (7 years, 9 months ago)

yes exactly like pastys. the only difference is the cornmeal and the ratio of the mix is a little different. but the plate method is the same.
ND, it might. but ive never let a plate go long enough after 98% colonization. im generally blending it up into LI by then.

im not sure about layering. but, my next trial will be mixing both together. some pda and some cornmeal. maybe make the best of both worlds. in the end its always about finding the right ratio. anything and everything can be done if you find the right mix.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
Offlinetombosley8
Full on... Bossley Baggins
 User Gallery


Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 1 hour
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23103672 - 04/10/16 02:31 PM (7 years, 9 months ago)

Do your cornmeal/brf plates come out pretty soft? Or should they be nearly as hard as agar?


--------------------


Extras: Filter Print Post Top
OfflinePsiloPsychIn
PsiloPsychIn
I'm a teapot User Gallery


Registered: 06/17/14
Posts: 8,182
Loc: up north
Last seen: 2 days, 23 hours
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: tombosley8]
    #23104149 - 04/10/16 05:16 PM (7 years, 9 months ago)

Eat can answer this also but in my experience they should be nearly as hard as agar.


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: PsiloPsychIn]
    #23104867 - 04/10/16 08:48 PM (7 years, 9 months ago)

yeah like agar.

if your finding they are too soft. you can always boil the cornmeal/water a little longer before pouring it into the plate. when i pour it, its a pasty consistency.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Edited by eatyualive (04/10/16 08:49 PM)


Extras: Filter Print Post Top
Offlinethisisfielding
the dude

Registered: 01/30/15
Posts: 178
Last seen: 7 years, 7 months
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23114658 - 04/13/16 08:09 PM (7 years, 9 months ago)

So I have two plates ready to blend for 25-ish jars. Would it be better to combine them together and blend or do them individually? How easy is it to see contamination on these plates - if by the time I blend its only white mycelium growth should I be good to go?


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: thisisfielding]
    #23116358 - 04/14/16 09:12 AM (7 years, 9 months ago)

i would do each separately. and one plate should be enough for 25 quart jars of grain. how many transfers have you done? if you have done a few and it looks clean then yes it should be fine. and yes you can see contams just like an agar plate. its a little easier if your using the colored cornmeal or a food coloring with your cornmeal if you can't find the blue cornmeal. its not really any different than using regular agar plates. just a little more nutritious and it colonizes a tad bit slower. but the growth is vigorous. i often will transfer from regular agar plates like PDA to cornmeal and see an explosion of robust growth.

use a regular mouth half pint jar. fill it half full with water. pc 20 mins at 15psi. once cool take into your SAB. wipe jars and plate with 70% iso. then loosen both lids. lift both lids simultaneously as you tiger drop your entire plate into the blender jar. put the lid of the blender jar back on. then blend it up on your blender base. wipe with iso again before taking it back into the sab. In a SAB i often do my blend, then clean all the jars and bring them into the SAB once the jar is ready for inoculation. in a shmuv, i clean all the quarts of spawn with iso first then put them in. bring in the blender jar/plate and do my drop. then blend it up on my base. then wipe with iso and bring it back into the shmuv.

either way works depending on what you have to work with.

its also wise to have two blender bases. you can get those at walmart in a repair kit for like 10$. it comes with a blender blade, black base, 2 gaskets. its often hidden on the upper shelf near the oster blenders. check your local walmart stock prior to going there because they generally list it in stock online. it will save you the trip.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Edited by eatyualive (04/14/16 06:34 PM)


Extras: Filter Print Post Top
Offlinethisisfielding
the dude

Registered: 01/30/15
Posts: 178
Last seen: 7 years, 7 months
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23117811 - 04/14/16 05:31 PM (7 years, 9 months ago)

Wow, thanks for the thorough response Eat! I've done 3 transfers from agar and then the final to the cornmeal plates - they look great but Im not familiar with using cornmeal so I don't know how easy its to spot contams. Is there a reason why we use them before they hit the sides of the container? I just ask because I have one of my plates that just hit one of the sides, healthy growth none the less. I have way more LI then I need then if 1 plate does 40 jars haha. Do you have a flowhood too? I always feel so paranoid using my flowhood and almost feel safer using a SAB.


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: thisisfielding]
    #23117880 - 04/14/16 05:50 PM (7 years, 9 months ago)

just in case there are any contams on the edge. ive also used plates that are fully colonized with no issues. but you don't want to let it go too far up the side of the plate.

nope i use a shmuvbox or a SAB. depends on how many jars im doing at once. if its under 20 then maybe a SAB. If its more Ill do the shmuvbox because i can fit many more jars in the 55 gallon bag.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
Offlinethisisfielding
the dude

Registered: 01/30/15
Posts: 178
Last seen: 7 years, 7 months
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23118026 - 04/14/16 06:23 PM (7 years, 9 months ago)

So I see you recommend using a pint jar / filling it half full so ~8 oz. I thought you were doing 1/4 a pint per plate in the original tek. I would prefer speed over quantity if it comes down that unless you think that theres no difference between the two.


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: thisisfielding]
    #23118064 - 04/14/16 06:34 PM (7 years, 9 months ago)

sorry i mis typed. regular mouth half pint jar.

yes 1/4 pint. i fixed it.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
Offlinethisisfielding
the dude

Registered: 01/30/15
Posts: 178
Last seen: 7 years, 7 months
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23118108 - 04/14/16 06:44 PM (7 years, 9 months ago)

Quote:

eatyualive said:
sorry i mis typed. regular mouth half pint jar.

yes 1/4 pint. i fixed it.



Perfect! Got it going :smile:. I just made some agar plates of the texas orange cap print you sent me, after a few transfers it'll be making it to cornmeal. I'm really interested in finding aggressive growers and I heard the texs are.


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: thisisfielding]
    #23118660 - 04/14/16 09:00 PM (7 years, 9 months ago)

yes they definitely are.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
Invisiblethe_r3dz
Trich Propagator
 User Gallery

Registered: 03/26/15
Posts: 5,709
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23118837 - 04/14/16 09:46 PM (7 years, 9 months ago)

Wow you use the shmuvbox? I always sorta thought that was kind of a ridiculous thing, don't think I've seen anyone else using it.
But knowing you and how many you have out there it's probably your tek :P so many times I'll look back at a tek that I read when I first registered and realized it was yours all along.
When did you get your TC tag?

How many jars can you actually fit into the shmuv? I got myself a gigantic (mostly tall) SAB and I love it. I can do huge bags g2g or  24 jars at a time.


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: the_r3dz]
    #23121293 - 04/15/16 04:04 PM (7 years, 9 months ago)

yes i prefer a shmuv over everything else. i can fit 50 quarts in a shmuv so all work can be done in one sitting. i also traded my flowhood in for a shmuvbox. it packs up nice and small into the box. and its inconspicuous. not big and bulky like a flowhood or a 110 quart sab with 5" arm holes cut in it. try hiding a tub that big somewhere conveniently.

i guess i got a tc tag when they invented the tc tag. i logged in one day and had it. but even back then i didn't post much here. i don't recall when that was. been a tc longer than many of the members on this forum have been registered.

i basically had a total post count of 1500 posts in 2013-2014 when i logged back into this forum from a 5 year break.

stealth is key. being able to walk into a grow room and not be able to tell its a grow room is ideal.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery


Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23121315 - 04/15/16 04:09 PM (7 years, 9 months ago)

Remember that youtube vid you posted some days ago? About those guys working with a shmux? I loved it, it really is an awesome thing.
Do you know if I can get that blower (or whatever it's called) off the internet?


Extras: Filter Print Post Top
Invisibleeatyualive
Eat's You Alive :)
 User Gallery


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: Josex]
    #23121331 - 04/15/16 04:12 PM (7 years, 9 months ago)

its a room size hepa unit. you buy the 99% true type hepa for it. unit cost about 35-40$. you can get all different types. but you want the out take to be on the top of the unit. odd shaped units don't fare to well.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery


Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Cornmeal Mush Plate Liquid Inoculation Trials [Re: eatyualive]
    #23121359 - 04/15/16 04:21 PM (7 years, 9 months ago)

Quote:

eatyualive said:
its a room size hepa unit. you buy the 99% true type hepa for it. unit cost about 35-40$. you can get all different types. but you want the out take to be on the top of the unit. odd shaped units don't fare to well.



That sounds like Mandarin to me right now :lol:, I'll have a gander at that link you sent me, thanks a lot!


Extras: Filter Print Post Top
OfflineGlazedHazels
Reality Outran Apprehension

Registered: 08/12/11
Posts: 199
Last seen: 2 months, 6 days
Re: Cornmeal Plate Liquid Inoculation [Re: eatyualive]
    #23762882 - 10/23/16 12:13 AM (7 years, 3 months ago)

Can the cornmeal replace all need for agar?

How much faster than blended agar is this?


Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,068
Trusted Cultivator
Re: Cornmeal Plate Liquid Inoculation [Re: GlazedHazels]
    #23763229 - 10/23/16 04:52 AM (7 years, 3 months ago)

Quote:

GlazedHazels said:
Can the cornmeal replace all need for agar?

How much faster than blended agar is this?




Any medium that can be reasonably colonized and inoculated cleanly and 'cut' into can be used in place of agar.

Look up 'brown rice flour agar'

People have been slurying brown rice flour and water together and putting it in vessels for sterilization for a long time now.

Most don't recommend it. Agar is just so nice and easy to cut into compared to mediums that don't dissolve in water.

It is by no means a 'replacement' for the convenience of agar.

But yeah as long as  you don't mind a little extra work when slicing into it for transfers you can use corn meal plates for all your culturing.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | Next >

Shop: North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* sterilizing hand blender - for liquid inoculant ohmatic 2,039 2 01/25/21 08:36 AM
by HappinessStan
* Inoculating with mushed up shroom tissue..?
( 1 2 all )
Openminded 7,260 34 06/26/17 09:37 AM
by bodhisatta
* Re: Does liquid mycelium do okay in light? Anonymous 1,104 3 12/26/99 10:06 PM
by Anonymous
* LC ml inoculant to a spawnbag. What do you use? Bobo 1,095 6 07/14/05 05:16 AM
by IGnosticAbhorI
* how to use cardboard cultures to inoculate grain jars and how to use a liquid culture to inoculate lordoftheshroomz 6,510 12 05/08/05 03:55 PM
by lordoftheshroomz
* WBS...agar or liquid knock up? ADoobie 1,226 4 01/30/05 01:21 AM
by ADoobie
* Liquid culture inoculation...adds water to grain jars GnuBobo 10,955 7 05/04/05 12:57 AM
by down44
* PF Cake to Liquid culture transfer? Rohypnol 2,713 5 10/25/03 05:05 PM
by Hanky

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
24,641 topic views. 43 members, 347 guests and 52 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.008 seconds on 15 queries.