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OfflineLuckyLucci
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Please tell me these are not contaminated
    #22247551 - 09/16/15 07:21 PM (8 years, 4 months ago)


They are 2 separate jars from the same batch. I was about to case or use them for a G2G when I saw this. I also believe these are the same jars I shook approximately a week ago at 100% colonization and they were bruising blue all over when I shook them. This makes me want to case/fruit all the jars I have in this batch if it is a contamination.


Edited by LuckyLucci (09/16/15 07:41 PM)


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InvisibleNobler Hino
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Re: Please tell me these are not contaminated [Re: LuckyLucci]
    #22247787 - 09/16/15 08:04 PM (8 years, 4 months ago)

Yeah looks rough, I'd toss it


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OfflineLuckyLucci
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Re: Please tell me these are not contaminated [Re: Nobler Hino]
    #22247824 - 09/16/15 08:12 PM (8 years, 4 months ago)

sad, it was all good just a week ago. What type of contam might it be?


Edited by LuckyLucci (09/16/15 08:36 PM)


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Offlineconfuzzed
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Re: Please tell me these are not contaminated [Re: LuckyLucci]
    #22248423 - 09/16/15 09:30 PM (8 years, 4 months ago)

Definite gone'er!

Hard to tell with just pics, could be trichoderma and/or could be bacterial...

If I had to guess I'd say bacterial from the looks.

Good luck :thumbup:


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OfflineLuckyLucci
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Re: Please tell me these are not contaminated [Re: confuzzed]
    #22248860 - 09/16/15 11:31 PM (8 years, 4 months ago)

I was so clean/sterile. I can't see these jars getting fully colonized then a month later get contaminated. I haven't opened them at all yet. Could it be a fruits or pins having a hard time forming after disturbing the mycelium? I probably wouldn't be able to smell the contam if I did open them, considering the contam is at the jars bottom, what do ya'll think?


Edited by LuckyLucci (09/16/15 11:38 PM)


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InvisibleNobler Hino
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Re: Please tell me these are not contaminated [Re: LuckyLucci]
    #22249784 - 09/17/15 07:21 AM (8 years, 4 months ago)

Pop the lid and smell the ass like aroma


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"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
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OfflineLuckyLucci
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Re: Please tell me these are not contaminated [Re: Nobler Hino]
    #22249831 - 09/17/15 07:45 AM (8 years, 4 months ago)

Lol maybe I have slick been picking up on the smell


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InvisibleNobler Hino
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Re: Please tell me these are not contaminated [Re: LuckyLucci]
    #22251051 - 09/17/15 12:40 PM (8 years, 4 months ago)

How did you prepare your jar? Just curious


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"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
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InvisibleMad Season
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Re: Please tell me these are not contaminated [Re: LuckyLucci]
    #22253469 - 09/17/15 09:23 PM (8 years, 4 months ago)

Quote:

LuckyLucci said:
I was so clean/sterile.



Did you inoculate with clean agar? Or dirty spores? Did you get your jars to an internal temp of 250+F for 2+ hours (15-20 psi) or 270+ for 1 hour (27+ psi)?

Did you inoculate in a still air box? Are your lids done with synthetic filter discs?

I bet there's much more to improve on. Click my improve your sterile techniques link in my signature as well :smile:


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Edited by Mad Season (09/17/15 09:24 PM)


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OfflineLuckyLucci
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Re: Please tell me these are not contaminated [Re: Mad Season]
    #22254639 - 09/18/15 06:13 AM (8 years, 4 months ago)

Pressure cooked at 15psi for 90 minutes. But that's true I could've improved in plenty areas


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InvisibleNobler Hino
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Re: Please tell me these are not contaminated [Re: LuckyLucci]
    #22254700 - 09/18/15 06:40 AM (8 years, 4 months ago)

Contams only make you a better grower, I had to toss one of mine a week ago that looks similar to yours only mine was brf cake. I started seeing black juice on the side like yours. Anyways better luck next time partner


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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Invisiblemicro
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Re: Please tell me these are not contaminated [Re: confuzzed]
    #22255345 - 09/18/15 09:38 AM (8 years, 4 months ago)

Quote:

confuzzed said:
Definite gone'er!

Hard to tell with just pics, could be trichoderma and/or could be bacterial...

If I had to guess I'd say bacterial from the looks.




Yeah, I agree with bacterial. Not trichoderma.


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OfflineLuckyLucci
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Re: Please tell me these are not contaminated [Re: micro]
    #22256888 - 09/18/15 04:12 PM (8 years, 4 months ago)

Thanks for  clarifying it is probably bacterial. I have yet to open them, they're sitting on my dining table. I'm pasteurize alder in the oven as I type. Any advice on treating itt because I'm going to try spawn it regardless, even I have to pick it off, use h2o2, or sprinkle antibiotics on it lol. Btw they are azurescens.


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Invisiblemicro
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Re: Please tell me these are not contaminated [Re: LuckyLucci]
    #22258223 - 09/18/15 09:22 PM (8 years, 4 months ago)

One would usually mix antibiotics in with the substrate. My recommendation in this case, if you need to save it (i.e. no more spores or inoculum) the best thing to do might be confirm bacterial and then transfer to ampicillin plates to get a sterile isolate.

I'm not going to say they would be impossible to fruit, though. I doubt the bacteria will EAT the mycelium itself but best to get rid of that part. I'm not sure about "dunking it" in an ampicillin (or similar) solution; I've never heard of that being done.

Best to keep the bacteriostatics to agar anyway, IMO.


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OfflineLuckyLucci
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Re: Please tell me these are not contaminated [Re: micro]
    #22263728 - 09/20/15 07:58 AM (8 years, 4 months ago)

Thanks bro, I'm looking into. I think it's worth a try especially if it hasn't been done.


Edited by LuckyLucci (09/20/15 07:59 AM)


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Invisiblemicro
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Re: Please tell me these are not contaminated [Re: LuckyLucci]
    #22266131 - 09/20/15 05:45 PM (8 years, 4 months ago)

No prob, just remember what you put in the mycelium will likely end up in the fruitbodies.


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