|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
LuckyLucci
Stranger


Registered: 03/06/15
Posts: 39
Last seen: 7 years, 2 months
|
Please tell me these are not contaminated
#22247551 - 09/16/15 07:21 PM (8 years, 4 months ago) |
|
|
 They are 2 separate jars from the same batch. I was about to case or use them for a G2G when I saw this. I also believe these are the same jars I shook approximately a week ago at 100% colonization and they were bruising blue all over when I shook them. This makes me want to case/fruit all the jars I have in this batch if it is a contamination.
Edited by LuckyLucci (09/16/15 07:41 PM)
|
Nobler Hino
a dojo and a forge?!



Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
|
Re: Please tell me these are not contaminated [Re: LuckyLucci]
#22247787 - 09/16/15 08:04 PM (8 years, 4 months ago) |
|
|
Yeah looks rough, I'd toss it
--------------------
   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
|
LuckyLucci
Stranger


Registered: 03/06/15
Posts: 39
Last seen: 7 years, 2 months
|
Re: Please tell me these are not contaminated [Re: Nobler Hino]
#22247824 - 09/16/15 08:12 PM (8 years, 4 months ago) |
|
|
sad, it was all good just a week ago. What type of contam might it be?
Edited by LuckyLucci (09/16/15 08:36 PM)
|
confuzzed
Stranger



Registered: 05/22/06
Posts: 298
Last seen: 3 years, 4 months
|
Re: Please tell me these are not contaminated [Re: LuckyLucci]
#22248423 - 09/16/15 09:30 PM (8 years, 4 months ago) |
|
|
Definite gone'er!
Hard to tell with just pics, could be trichoderma and/or could be bacterial...
If I had to guess I'd say bacterial from the looks.
Good luck
|
LuckyLucci
Stranger


Registered: 03/06/15
Posts: 39
Last seen: 7 years, 2 months
|
Re: Please tell me these are not contaminated [Re: confuzzed]
#22248860 - 09/16/15 11:31 PM (8 years, 4 months ago) |
|
|
I was so clean/sterile. I can't see these jars getting fully colonized then a month later get contaminated. I haven't opened them at all yet. Could it be a fruits or pins having a hard time forming after disturbing the mycelium? I probably wouldn't be able to smell the contam if I did open them, considering the contam is at the jars bottom, what do ya'll think?
Edited by LuckyLucci (09/16/15 11:38 PM)
|
Nobler Hino
a dojo and a forge?!



Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
|
Re: Please tell me these are not contaminated [Re: LuckyLucci]
#22249784 - 09/17/15 07:21 AM (8 years, 4 months ago) |
|
|
Pop the lid and smell the ass like aroma
--------------------
   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
|
LuckyLucci
Stranger


Registered: 03/06/15
Posts: 39
Last seen: 7 years, 2 months
|
Re: Please tell me these are not contaminated [Re: Nobler Hino]
#22249831 - 09/17/15 07:45 AM (8 years, 4 months ago) |
|
|
Lol maybe I have slick been picking up on the smell
|
Nobler Hino
a dojo and a forge?!



Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
|
Re: Please tell me these are not contaminated [Re: LuckyLucci]
#22251051 - 09/17/15 12:40 PM (8 years, 4 months ago) |
|
|
How did you prepare your jar? Just curious
--------------------
   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: Please tell me these are not contaminated [Re: LuckyLucci]
#22253469 - 09/17/15 09:23 PM (8 years, 4 months ago) |
|
|
Quote:
LuckyLucci said: I was so clean/sterile.
Did you inoculate with clean agar? Or dirty spores? Did you get your jars to an internal temp of 250+F for 2+ hours (15-20 psi) or 270+ for 1 hour (27+ psi)?
Did you inoculate in a still air box? Are your lids done with synthetic filter discs?
I bet there's much more to improve on. Click my improve your sterile techniques link in my signature as well
Edited by Mad Season (09/17/15 09:24 PM)
|
LuckyLucci
Stranger


Registered: 03/06/15
Posts: 39
Last seen: 7 years, 2 months
|
Re: Please tell me these are not contaminated [Re: Mad Season]
#22254639 - 09/18/15 06:13 AM (8 years, 4 months ago) |
|
|
Pressure cooked at 15psi for 90 minutes. But that's true I could've improved in plenty areas
|
Nobler Hino
a dojo and a forge?!



Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
|
Re: Please tell me these are not contaminated [Re: LuckyLucci]
#22254700 - 09/18/15 06:40 AM (8 years, 4 months ago) |
|
|
Contams only make you a better grower, I had to toss one of mine a week ago that looks similar to yours only mine was brf cake. I started seeing black juice on the side like yours. Anyways better luck next time partner
--------------------
   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: Please tell me these are not contaminated [Re: confuzzed]
#22255345 - 09/18/15 09:38 AM (8 years, 4 months ago) |
|
|
Quote:
confuzzed said: Definite gone'er!
Hard to tell with just pics, could be trichoderma and/or could be bacterial...
If I had to guess I'd say bacterial from the looks.
Yeah, I agree with bacterial. Not trichoderma.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
LuckyLucci
Stranger


Registered: 03/06/15
Posts: 39
Last seen: 7 years, 2 months
|
Re: Please tell me these are not contaminated [Re: micro]
#22256888 - 09/18/15 04:12 PM (8 years, 4 months ago) |
|
|
Thanks for clarifying it is probably bacterial. I have yet to open them, they're sitting on my dining table. I'm pasteurize alder in the oven as I type. Any advice on treating itt because I'm going to try spawn it regardless, even I have to pick it off, use h2o2, or sprinkle antibiotics on it lol. Btw they are azurescens.
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: Please tell me these are not contaminated [Re: LuckyLucci]
#22258223 - 09/18/15 09:22 PM (8 years, 4 months ago) |
|
|
One would usually mix antibiotics in with the substrate. My recommendation in this case, if you need to save it (i.e. no more spores or inoculum) the best thing to do might be confirm bacterial and then transfer to ampicillin plates to get a sterile isolate.
I'm not going to say they would be impossible to fruit, though. I doubt the bacteria will EAT the mycelium itself but best to get rid of that part. I'm not sure about "dunking it" in an ampicillin (or similar) solution; I've never heard of that being done.
Best to keep the bacteriostatics to agar anyway, IMO.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
LuckyLucci
Stranger


Registered: 03/06/15
Posts: 39
Last seen: 7 years, 2 months
|
Re: Please tell me these are not contaminated [Re: micro]
#22263728 - 09/20/15 07:58 AM (8 years, 4 months ago) |
|
|
Thanks bro, I'm looking into. I think it's worth a try especially if it hasn't been done.
Edited by LuckyLucci (09/20/15 07:59 AM)
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: Please tell me these are not contaminated [Re: LuckyLucci]
#22266131 - 09/20/15 05:45 PM (8 years, 4 months ago) |
|
|
No prob, just remember what you put in the mycelium will likely end up in the fruitbodies.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
|