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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: SAB Sterile Tek Video Series [Re: cheeseball]
#22091147 - 08/14/15 01:09 PM (8 years, 9 months ago) |
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Always open to suggestions for videos as well!
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22091171 - 08/14/15 01:17 PM (8 years, 9 months ago) |
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How about making slants?
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: SAB Sterile Tek Video Series [Re: Grey]
#22091467 - 08/14/15 02:23 PM (8 years, 9 months ago) |
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Quote:
Grey said: How about making slants?
I have $25 towards Amazon, can anyone link me the preferred culture tubes? I've never made slants, but it would be a great experience and help store some of the cultures I have been lucky enough to acquire.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22091585 - 08/14/15 02:57 PM (8 years, 9 months ago) |
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
Edited by Grey (08/14/15 03:15 PM)
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: SAB Sterile Tek Video Series [Re: Grey]
#22092944 - 08/14/15 10:29 PM (8 years, 8 months ago) |
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Quote:
Grey said: These are pretty cool and cheap. PP5 as well.
These are nice as well.
sweet the first ones are out of stock but the second ones arent and they are withing my price range. i'll order them now and make the vids when I can!
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22194968 - 09/05/15 10:40 PM (8 years, 8 months ago) |
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Quote:
In ur video where u make a li without a blender I wanted to ask u 2 ?s one when u squirt the water on to the pasty plate do u think if u took the syringe and scraped the mycelium on the agar would that make the li stronger since u would get more mycelium into the liquid or by doing that u would get agar in the li and that wouldent be good like would agitating the mycelium before sucking up the water make a better li ??? My second question is when u squirt the water into the jar how much water do you use??also after squirting the water back Into the jar would it be better to let it sit and allow the myc to grow like in a lc or is it better to use it rite away
a user had some questions, so here are the answers
yes, you can scrape the plates. make sure you flame sterilize your needles!
when creating LI, use the entire syringe. when creating LC, 1cc should be fine.
Always use LI ASAP.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,269
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22198311 - 09/06/15 05:17 PM (8 years, 8 months ago) |
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Why use LI's ASAP? I've stored mine in the fridge for a while and they were viable.
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Alphawolf
Psychonaut

Registered: 08/05/15
Posts: 87
Last seen: 6 years, 3 months
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U sed to ask u here so that's Wut I'm doing. When u say u used all of it do u mean u kept repeating the process of squirting and sucking the liquid from the agar to the jar???and when u say all of it u mean the agar too or just the mycelium is it okay if u get agar into the syring or do u avoid the agar and just suck up the mycelium??
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: SAB Sterile Tek Video Series [Re: Alphawolf]
#22198801 - 09/06/15 07:11 PM (8 years, 8 months ago) |
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Just one syringe worth. You just want mycelium but the agar won't hurt anything if you do get a little.
Since li is non nutritious, i prefer to use it all asap to ensure a vigorous healthy culture. This is just my preference. I like to keep the age of the mycelium to a minimum.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,269
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22244256 - 09/16/15 01:50 AM (8 years, 7 months ago) |
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How is the LI non-nutritious when there's agar involved? Wouldn't it just keep growing in the water like an LC?
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Quote:
stareatclouds said: How is the LI non-nutritious when there's agar involved? Wouldn't it just keep growing in the water like an LC?
Indeed, however, not all LI methods involve using the agar. If you use the power-wash or scrape, then it will be just water and mycelium.
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DivineO
Chillin'



Registered: 08/11/15
Posts: 110
Last seen: 7 years, 8 months
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22244609 - 09/16/15 07:11 AM (8 years, 7 months ago) |
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props on the nice post
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: SAB Sterile Tek Video Series [Re: DivineO]
#22245368 - 09/16/15 11:03 AM (8 years, 7 months ago) |
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thanks! I hope this thread stays active for a little bit this time so people don't feel the need to questions instead of just posting them here!
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,269
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22245429 - 09/16/15 11:12 AM (8 years, 7 months ago) |
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Oh yes, I forgot your version of LI here is different than the one I'm used to. My bad.
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Quote:
stareatclouds said: Oh yes, I forgot your version of LI here is different than the one I'm used to. My bad.
no need for an apology! I'd actually be pretty interested in seeing a plate getting blended up, then sat on a shelf for a few weeks to see what happens!
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,269
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22245577 - 09/16/15 11:42 AM (8 years, 7 months ago) |
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I did that, but left it for about 6-8 weeks, I think. The mycelium colonized at the very top of the water and basically formed a new agar wedge with more LI below it, floating around
I dropped that new wedge into a grain jar and used the LI to noc up other things. I wound up fucking everything up by G2Ging wrong so not sure how viable it was. The master spawn looked and smelled clean though.
I just put another PE tub into fruiting conditions with spawn I noc'd up with old LI as well. So far, so good. That one was probably out for 2 weeks in a cupboard.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,269
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Any chance you can record the process of swabbing PE gills? I believe my culture is already a clone so I don't want to take another clone. I'd love to see the process of swabbing gills!
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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I have some APE jars close to 100%. If I get any ape producers, I def will.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,269
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#22270189 - 09/21/15 04:00 PM (8 years, 7 months ago) |
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Sweet, I'll be looking forward to it!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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sticky for the week
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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