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Offlinegravybrain
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Registered: 07/01/13
Posts: 12
Loc: VT, USA
Last seen: 6 years, 9 months
Gravy's Grow Log/Monotub Questions
    #22243638 - 09/15/15 10:07 PM (8 years, 4 months ago)

I've been lurking around the forums for a couple years now, trying to follow all your sagacious advice, i.e., using the search function. Now it's time, I think, to reemerge, share my adventures, and invite you all to follow my future progress.

I've completed two very successful pf grows (Puerto Rico and GT, MS obviously), following the basic tek. Three successful grows if you count the time I fruited  schizophyllum commune:facepalm3:

Now I find myself with ten pf jars fully colonized and starting to pin in vitro, and no more perlite.



So I've decided to buy coir instead, and begin my journey into the world of bulk! I'm planning on crumbling my cakes into one of Pasty's Mini Monos, with Damion5050's coir sub.

I've just invested in a pc, built a SAB, visited my local Asian Market, and ordered all my lab goodies, so my agar adventure is about to begin as well.

I do have some questions before I begin, some of which seem to have highly contested answers when searched.

1. To case? Seems like the general response is "you can go for it, but it's not necessary." But everybody seems to do it regardless. Apparent Pros: bigger flushes, because of (ideal microclimate? Myc likes to pin on a semi-nutritious sub? Keeps sub hydrated? I'm seeing lots of hypotheses.) Cons: more opportunities for contams to strike (seems like a good enough reason to avoid?)

Which brings us to the next question,

2. A monotub, like a SGFC, is not a sterile environment, correct? You keep it clean, wash your hands, don't throw shit around, but it's more-or-less exposed to the air.
Does the polyfill act more as an air filter or as a way to dial in your tub's RH?
Do the thermophillic bacteria cultivated by the pasteurization process really keep contams at bay for the full 10+ days of colonization? Pasteurization mystifies me. (I know, I should read Stamets. It's in the mail!)

3. Black garbage bag? Do I wrap my tub in one? Some of you do, some of you don't. Is this something we used to do that we've found unnecessary?

4. Cakes, not grains (practice!) Anything special I should know/do? I've got a 27qt tub, so I'm planning on about 4qts of sub. Does this sound like too much/little for 10 pf cakes?

That's all for now, I've been composing this (fairly brief) post over the course of three hours, and am now too stoned to think of anything else to add.

Pics of my process to follow.
Thanks for any interest/advice!
-Gravy
Updated with pics of a couple of cakes yearning for freedom, and my freshly minted minimono!


Edited by gravybrain (09/16/15 10:20 AM)


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Gravy's Grow Log/Monotub Questions [Re: gravybrain]
    #22243833 - 09/15/15 10:54 PM (8 years, 4 months ago)

Quote:

gravybrain said:
So I've decided to buy coir instead, and begin my journey into the world of bulk! I'm planning on crumbling my cakes into one of Pasty's Mini Monos, with Damion5050's coir sub.

I've just invested in a pc, built a SAB, visited my local Asian Market, and ordered all my lab goodies, so my agar adventure is about to begin as well.



:woot:

Quote:

gravybrain said:
1. To case? Seems like the general response is "you can go for it, but it's not necessary." But everybody seems to do it regardless. Apparent Pros: bigger flushes, because of (ideal microclimate? Myc likes to pin on a semi-nutritious sub? Keeps sub hydrated? I'm seeing lots of hypotheses.) Cons: more opportunities for contams to strike (seems like a good enough reason to avoid?)



It's not necessary for cubes to fruit, but it does help create a good pinset. The casing layer is supposed to be non-nutritious (spaghnum peat moss/verm) and needs to be pH balanced(around 8) so mold spores will not readily grow on the surface.

Quote:

gravybrain said:
2. A monotub, like a SGFC, is not a sterile environment, correct? You keep it clean, wash your hands, don't throw shit around, but it's more-or-less exposed to the air.
Does the polyfill act more as an air filter or as a way to dial in your tub's RH?
Do the thermophillic bacteria cultivated by the pasteurization process really keep contams at bay for the full 10+ days of colonization? Pasteurization mystifies me. (I know, I should read Stamets. It's in the mail!)



Yes, fruiting is not done in a sterile environment. Those extra precautions are just to have peace of mind. If you have clean spawn, then you shouldn't have to worry about contams (that's why using agar is soo important). A lot of growers like to sterilize their coir/verm/gypsum mix via the "bucket tek", or you could pasteurize it properly, doesn't seem to make too much of a difference, it's up to you. The idea is to keep most beneficial bacteria alive, while killing off the bad bacteria, so your sub can colonize better. Frank's easy pasteurization tek is great! http://www.shroomery.org/forums/showflat.php/Number/17246844/fpart/all/vc/1

Quote:

gravybrain said:
3. Black garbage bag? Do I wrap my tub in one? Some of you do, some of you don't. Is this something we used to do that we've found unnecessary?



Make sure to line your tub with the black trash bag, it helps prevent side-pinning. As the sub shrinks after the first flush, the bag will shrink with it, so there are no gaps created between the sub and the wall of your tub (which would leave a microclimate for pins to form and would be difficult to harvest).

Quote:

gravybrain said:
4. Cakes, not grains (practice!) Anything special I should know/do? I've got a 27qt tub, so I'm planning on about 4qts of sub. Does this sound like too much/little for 10 pf cakes?



Sounds like a good ratio, a 27qt tub isn't too big. The more cakes, the better! :wink:

Also, make sure to use a cheese grater to get them to the smallest pieces possible before mixing with your bulk sub, colonization times will greatly increase.
Quote:

gravybrain said:
That's all for now, I've been composing this (fairly brief) post over the course of three hours, and am now too stoned to think of anything else to add.

Pics of my process to follow.
Thanks for any interest/advice!
-Gravy



:awethumb:


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Offlinegravybrain
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Posts: 12
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Re: Gravy's Grow Log/Monotub Questions [Re: Psilosoulful]
    #22243964 - 09/15/15 11:32 PM (8 years, 4 months ago)

Thanks for the comprehensive response, my dude!
As for lining the tubs, I'm for sure doing so. I was wondering more about wrapping the whole tub in a garbage bag during the colonization period, a la Damion's Bucket tek.

Quote:

STEP 13

Now this step is optional but I do it.  I cover the entire tub with a black garbage bag and place it on a shelf of wherever you decide to.  Come back 10 days later and take a look at it and see how far along it is.




Some folks seem to just throw tape over the holes and not worry about light one way or the other until FAE is introduced, others switch from a solid to a clear lid when they introduce fruiting. As I understand it, light comes last as far as pinning triggers go, so it shouldn't make too much of a difference, right? My brf jars have grown just fine in ambient daylight, anyway.


--------------------
Sleep is the greatest drug of all. The worst side effect is that when you wake up, it's tomorrow.
Naps are the solution.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Gravy's Grow Log/Monotub Questions [Re: gravybrain]
    #22244019 - 09/15/15 11:53 PM (8 years, 4 months ago)

Whoops, read that part wrong. Most cultivators have said that they just leave their tubs on a shelf or in the corner of a room in natural daylight to colonize. Light is supposed to be beneficial to all stages of the growing process. I've left tubs uncovered in low light settings vs totally covered . The differences in colonization times are very minimal IME. The factor that matters the most is having clean spawn, or better yet, a strong culture/isolate that will rip through the bulk sub regardless of the level of lighting. :awesomenod:


Edited by Psilosoulful (09/15/15 11:53 PM)


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Invisiblefilthyknees
no coincidence
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Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: Gravy's Grow Log/Monotub Questions [Re: gravybrain]
    #22244429 - 09/16/15 05:30 AM (8 years, 4 months ago)

ambient daylight is perfect. I've heard it said full colonization is a primary pinning trigger and light is a secondary.

seems like you're on the right path :smile: :goodluck:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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