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Invisiblerams
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Bleaching powder and water solution for SAB?
    #22239934 - 09/15/15 07:15 AM (8 years, 4 months ago)

I bought one leter liquid bleach but the vendor told me later that it has 4 percent sodium hypochlorite. Now I am totally puzzled that how to make it 10 percent to bomb my SAB. I also have 2 kgs of Bleaching powder. If I desolve 100 gm of bleaching powder to 900 ml of water will it serve the purpose or do I need to get the liquid bleach ratio corrected for the same.


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Invisiblewowimflabbergasted
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Re: Bleaching powder and water solution for SAB? [Re: rams]
    #22239939 - 09/15/15 07:19 AM (8 years, 4 months ago)

Just use soap and water.


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OfflinePussyFart
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Re: Bleaching powder and water solution for SAB? [Re: rams]
    #22239940 - 09/15/15 07:19 AM (8 years, 4 months ago)

Quote:

rams said:
I bought one leter liquid bleach but the vendor told me later that it has 4 percent sodium hypochlorite. Now I am totally puzzled that how to make it 10 percent to bomb my SAB. I also have 2 kgs of Bleaching powder. If I desolve 100 gm of bleaching powder to 900 ml of water will it serve the purpose or do I need to get the liquid bleach ratio corrected for the same.



Mix a 5% mixture, it will work the same.....ratios are not important, you can actually just use soap and water....

The point of a SAB(Still Air Box) is to create a draft free environment....not a sterile one.


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InvisibleInocuole
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Re: Bleaching powder and water solution for SAB? [Re: PussyFart]
    #22240075 - 09/15/15 08:01 AM (8 years, 4 months ago)

Most of the time I just use water. :shrug:


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Re: Bleaching powder and water solution for SAB? [Re: Inocuole]
    #22240343 - 09/15/15 09:08 AM (8 years, 4 months ago)

I have read a lot from experts like you guys here who just use soap water. however for a first timer (Using SAB and doing agar work)like me its a moment of nervousness. Misting my SAB and the Clean room with bleach or other disinfectant may not be necessary for experts but I just want to be on a safer side and do not want to give failure a chance.
How good is the idea of using just soap and water inside the SAB and sanitizing the Clean room instead?


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Invisiblewowimflabbergasted
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Re: Bleaching powder and water solution for SAB? [Re: rams]
    #22240352 - 09/15/15 09:12 AM (8 years, 4 months ago)

Clean the room you're in of course, but that's not the main job of a SAB as pussyfart said earlier. If you want, use a spray bottle with 70% iso alcohol inside your SAB just don't light any fires in there... :onfire:


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Re: Bleaching powder and water solution for SAB? [Re: wowimflabbergasted]
    #22240397 - 09/15/15 09:24 AM (8 years, 4 months ago)

:whathesaid:


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Re: Bleaching powder and water solution for SAB? [Re: taGyo]
    #22240453 - 09/15/15 09:36 AM (8 years, 4 months ago)

I use a really weak bleach solution in the bottom on my towel, and I use 70% iso for everything else....(walls, tools, jars, gloves, etc...)

I have no need to flame sterilize anything, ever, so I never have to worry about ignition problems....lol.

If your wondering how on earth can I get away without flaming anything, I order my scalpel blades by the thousands off ebay....individually wrapped and sterilized....and cheap as shit too....


Edited by PussyFart (09/15/15 09:37 AM)


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Invisiblewowimflabbergasted
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Re: Bleaching powder and water solution for SAB? [Re: PussyFart]
    #22240456 - 09/15/15 09:38 AM (8 years, 4 months ago)

Pre-sterilized tools are great.


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InvisibleInocuole
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Re: Bleaching powder and water solution for SAB? [Re: wowimflabbergasted]
    #22240800 - 09/15/15 10:56 AM (8 years, 4 months ago)

You're not going to get better success by bleaching anything.  That's not how the SAB works.  Bleach won't even kill everything.  It matters more that it's wet than it does that it's killing stuff.


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Re: Bleaching powder and water solution for SAB? [Re: wowimflabbergasted]
    #22240844 - 09/15/15 11:06 AM (8 years, 4 months ago)

Quote:

wowimflabbergasted said:
Clean the room you're in of course, but that's not the main job of a SAB as pussyfart said earlier. If you want, use a spray bottle with 70% iso alcohol inside your SAB just don't light any fires in there... :onfire:




This.  Also, don't be tempted to get 98% iso if you see it at the store, it's less effective for mycological purposes.

I will say that I do flame in my SAB after spraying down with iso, but not before placing the SAB with the lid slightly cracked in a draft free room so the alcohol evaporates first.  Bleach eliminates this problem, but I don't like having excess moisture in there because I fumble with equipment when the gloves get wet.

As others have said, it's really not necessary to get your SAB sterile.  It's more a peace of mind thing.


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Re: Bleaching powder and water solution for SAB? [Re: shroombasa]
    #22240874 - 09/15/15 11:11 AM (8 years, 4 months ago)

Quote:

shroombasa said:
I will say that I do flame in my SAB after spraying down with iso, but not before placing the SAB with the lid slightly cracked in a draft free room so the alcohol evaporates first. 



Yes, because the fumes are not flamable in the least bit.... :rolleyes:

And what good did it do you if you just let it evaporate?

Now the walls are dry, and nothing can really stick to them.......


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Re: Bleaching powder and water solution for SAB? [Re: PussyFart]
    #22240893 - 09/15/15 11:16 AM (8 years, 4 months ago)

Quote:

PussyFart said:
Quote:

shroombasa said:
I will say that I do flame in my SAB after spraying down with iso, but not before placing the SAB with the lid slightly cracked in a draft free room so the alcohol evaporates first. 



Yes, because the fumes are not flamable in the least bit.... :rolleyes:

And what good did it do you if you just let it evaporate?

Now the walls are dry, and nothing can really stick to them.......



Hey, pussyfart.  Definitely not disagreeing with a TC, but I was suggesting letting the fumes evaporate in an area that won't be exposed to an open flame, hence, letting them dry out in a draft free room so contams don't enter as fumes are released.


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Re: Bleaching powder and water solution for SAB? [Re: shroombasa]
    #22240943 - 09/15/15 11:28 AM (8 years, 4 months ago)

How will the fumes be released if there's no draft?


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Re: Bleaching powder and water solution for SAB? [Re: Inocuole]
    #22240975 - 09/15/15 11:40 AM (8 years, 4 months ago)

How do u know all the fumes are gone? You cannot see them. And leaving the "lid cracked" in a "draft free" environment is not going rid the box of fumes...

Flaming in your SAB is terrible practice, plus you are creating heat currents in your SAB, which kind of defeats the purpose of having a still air, draft free box....


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Re: Bleaching powder and water solution for SAB? [Re: PussyFart]
    #22241000 - 09/15/15 11:44 AM (8 years, 4 months ago)

Don't flame in your SAB dude. :shitdude:


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Re: Bleaching powder and water solution for SAB? [Re: wowimflabbergasted]
    #22248404 - 09/16/15 09:26 PM (8 years, 4 months ago)

Yeah, One thing I know is flame inside your SAB only if you want to scare the shit out of your neighbors with some fire work.. hahaha


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