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OfflineBumbaa
Lol You Can Change this

Registered: 08/29/15
Posts: 295
Last seen: 1 year, 2 months
Slow colonization of BRF cake.
    #22236342 - 09/14/15 12:11 PM (8 years, 4 months ago)

Hello,about three weeks ago I inoculated eight half pint 1/2 wide mouth BRF cake utilizing the PF tek method. I've let them sit without any type of sunlight for three weeks. It took them about 7-9 days to see any type of mycelium, expect for three of them that has been discarded after showing no sign of mycelium after three weeks, one of them was contaminated by green mold.

Temperature was in the 20 degree Celsius, recently drop to 16 degree celcius. I assume that a temperature of 20 degree celcius is O.K for mycelium growth,since I've read that you can incubate them at room temperature.


Jar #1:











I would say this jar is about 40% colonized,I can see moisture droplet on the side of the jar. Some place are easy to see trough the mycelium.



Jar #2:








This one is about 30-40%,the first picture shows mycelium that is very thin. That jar shows a lot of moisture also.



Jar #3:





This is my favorite jar,it was the first one to grow anything in a nice dime spot and expanded to this nice fluffy snow white mycelium growth. I would say about 35%.



Jar #4:





This jar shows that amount of mycelium after 3 weeks of inoculation day.


Jar #5:








This jar shows a lot of moisture droplet on the side of the jar,thin mycelium on the side, thick mycelium on the bottom. About 60% colonized.



I would like any type of feedback. What do you guys think,normal growth speed? If not, why is that?

Cheers,

Bumbaa



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OfflineBumbaa
Lol You Can Change this

Registered: 08/29/15
Posts: 295
Last seen: 1 year, 2 months
Re: Slow colonization of BRF cake. [Re: Bumbaa]
    #22242445 - 09/15/15 05:43 PM (8 years, 4 months ago)

Bump


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OfflineNDStepp84
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Registered: 04/23/15
Posts: 4,956
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Re: Slow colonization of BRF cake. [Re: Bumbaa]
    #22243008 - 09/15/15 07:38 PM (8 years, 4 months ago)

Could be a number of things, genetics, moisture content, contamination to name a few. As long as they are still colonizing and not completely stalled ride it out. Take a sharpie and trace the growth on the jar. Patience is key, mushrooms work on their time. Rule a couple things out, do you have tape or anything on the inoculation holes that could hinder GE? That verm looks on the course side, which will work but may hold less water making your mix on the wet side. Did you add water to the verm first then mix in the BRF? Fine verm is preferred, especially for the filter layer on top.


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Invisiblewowimflabbergasted
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Registered: 07/16/12
Posts: 18,918
Re: Slow colonization of BRF cake. [Re: NDStepp84]
    #22243304 - 09/15/15 08:41 PM (8 years, 4 months ago)

The larger verm isn't helping things at all.


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Invisibleelasticaltiger
Like Tigers in Coitus
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Registered: 06/24/13
Posts: 8,064
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Re: Slow colonization of BRF cake. [Re: wowimflabbergasted]
    #22243320 - 09/15/15 08:46 PM (8 years, 4 months ago)

Did you inoculate your jars in open air?


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OfflineBumbaa
Lol You Can Change this

Registered: 08/29/15
Posts: 295
Last seen: 1 year, 2 months
Re: Slow colonization of BRF cake. [Re: elasticaltiger]
    #22243338 - 09/15/15 08:48 PM (8 years, 4 months ago)

Quote:

NDStepp84 said:
Could be a number of things, genetics, moisture content, contamination to name a few. As long as they are still colonizing and not completely stalled ride it out. Take a sharpie and trace the growth on the jar. Patience is key, mushrooms work on their time. Rule a couple things out, do you have tape or anything on the inoculation holes that could hinder GE? That verm looks on the course side, which will work but may hold less water making your mix on the wet side. Did you add water to the verm first then mix in the BRF? Fine verm is preferred, especially for the filter layer on top.




No tape on the holes,I used promix vermiculite bought from Canadian tire. Yes I mixed the verm with water before adding BRF. The filter layer on top utulize the same vermiculite as the mixture.


Quote:

wowimflabbergasted said:
The larger verm isn't helping things at all.




So I'm assuming this vermiculite is not fine enough?

Quote:

elasticaltiger said:
Did you inoculate your jars in open air?




I inoculated them using the 4 holes technique in open air. I had no still air box.


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Invisibleblew42
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Registered: 01/14/15
Posts: 264
Loc: Wonderland Flag
Re: Slow colonization of BRF cake. [Re: Bumbaa]
    #22243595 - 09/15/15 09:53 PM (8 years, 4 months ago)

Your substrate looks a bit odd for PF tek; it should be finer and less pellet-like. Make sure you're using medium horticultural grade Verm. Also careful about picking up/tilting the jars like that for pictures; the cakes shrink as the mycelium colonized and contaminated Verm can fall and contaminate the healthy myc. Other than that the growth looks healthy to me , just be patient! Im pushing over a month with my current jars and just one of them has reached 100%, but they're all healthy! Sending good luck your way my man


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OfflineBumbaa
Lol You Can Change this


Registered: 08/29/15
Posts: 295
Last seen: 1 year, 2 months
Re: Slow colonization of BRF cake. [Re: blew42]
    #22244993 - 09/16/15 09:23 AM (8 years, 4 months ago)

Quote:

blew42 said:
Your substrate looks a bit odd for PF tek; it should be finer and less pellet-like. Make sure you're using medium horticultural grade Verm. Also careful about picking up/tilting the jars like that for pictures; the cakes shrink as the mycelium colonized and contaminated Verm can fall and contaminate the healthy myc. Other than that the growth looks healthy to me , just be patient! Im pushing over a month with my current jars and just one of them has reached 100%, but they're all healthy! Sending good luck your way my man





I think I understand,I should user some finer verm and be more carefull with the jar.

Thanks!


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