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OfflineQuit The Cult
World is yours

Registered: 11/12/14
Posts: 265
Last seen: 8 years, 3 months
Reusing a spore syringe?
    #22235921 - 09/14/15 10:23 AM (8 years, 4 months ago)

Hey guys.

I inoculated 3 rye bags about ten days ago and two of the three bags contaminated. I have two more bags that I need to still inoculate. But will it be safe to inoculate with spore syringes that I've already used?

What is the best way to make sure the used syringes will be clean enough?

Thanks.


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Invisiblewowimflabbergasted
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22235943 - 09/14/15 10:29 AM (8 years, 4 months ago)

Agar.


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OfflineQuit The Cult
World is yours

Registered: 11/12/14
Posts: 265
Last seen: 8 years, 3 months
Re: Reusing a spore syringe? [Re: wowimflabbergasted]
    #22237436 - 09/14/15 04:23 PM (8 years, 4 months ago)

What exactly is Agar? And how much of it would I need to purchase? I just rub Agar on the needle? I don't get it.

Can I buy Agar in stores or only online?


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InvisibleUncleFester
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22237638 - 09/14/15 05:18 PM (8 years, 4 months ago)

Use the shroomery.org search engine doode
:hahyeahwoo:


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InvisibleUncleFester
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22237656 - 09/14/15 05:22 PM (8 years, 4 months ago)

If the spores came from a place with a good reputation for clean syringes then you're likely doing non-sterile inoculations. Sterility is the most important thing.


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OfflineQuit The Cult
World is yours

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Re: Reusing a spore syringe? [Re: UncleFester]
    #22237682 - 09/14/15 05:27 PM (8 years, 4 months ago)

Quote:

UncleFester said:
Use the shroomery.org search engine doode
:hahyeahwoo:



The point of these sections is for people to post their grows and ask questions right? Telling me to use the search function doesn't help me.


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OfflineiSmkGrnBud
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22237689 - 09/14/15 05:30 PM (8 years, 4 months ago)

You asked. He answered. Search agar. You'll find tons of threads about it. :thumbup:


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“The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi


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OfflineQuit The Cult
World is yours

Registered: 11/12/14
Posts: 265
Last seen: 8 years, 3 months
Re: Reusing a spore syringe? [Re: iSmkGrnBud]
    #22237700 - 09/14/15 05:32 PM (8 years, 4 months ago)

SMH. Thanks for the help guys I appreciate you telling me to use the search function.

Because fuck knows I didn't already try that and come up with more confusing shit that seemed unrelated to what I was trying to learn.

Thought this place was supposed to be helpful. Maybe I'll get lucky and find one helpful member.


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InvisibleUncleFester
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22237739 - 09/14/15 05:38 PM (8 years, 4 months ago)

I understand being spoon-fed info is easy BUT you like many others have similar questions which have been answered here almost daily. You don't have to come to the fourms and ask when you can look up an agar write up such as pastywhyte's easy agar tek


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InvisibleUncleFester
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22237764 - 09/14/15 05:42 PM (8 years, 4 months ago)

Quote:

Quit The Cult said:
SMH. Thanks for the help guys I appreciate you telling me to use the search function.

Because fuck knows I didn't already try that and come up with more confusing shit that seemed unrelated to what I was trying to learn.

Thought this place was supposed to be helpful. Maybe I'll get lucky and find one helpful member.




Bro we're trying to help you.. Before you Lead you must Read. Knowledge is power.


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Offlinesulliv23
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22237891 - 09/14/15 06:06 PM (8 years, 4 months ago)

Quote:

Quit The Cult said:
What exactly is Agar? And how much of it would I need to purchase? I just rub Agar on the needle? I don't get it.

Can I buy Agar in stores or only online?




Agar is just a culture medium. You can buy it on amazon already poured into petri dishes. He is saying you should use the new syringes to put a few drops on the agar and see if 1) mycelium grows 2)Contams take over. If contams take over, re-do the syringes and try on the agar again...

But for real you should do some more reading. Agar can be kind of difficult, especially if you live in a hot climate and want to have it shipped. If you use a glove box and sterilize everything extra well, you shouldn't have a problem. I'm positive there are guides to reusing syringes. Sterilizing everything is a bitch but if you take your time and do it right the first time, you won't have to re-do it. Good luck sir.


Edited by sulliv23 (09/14/15 06:09 PM)


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InvisibleGoblinKing
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Re: Reusing a spore syringe? [Re: sulliv23]
    #22239061 - 09/14/15 10:41 PM (8 years, 4 months ago)

Flame steralize, squeeze some out, re steralize then all there is left to do is


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Edited by GoblinKing (09/14/15 11:08 PM)


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Invisibleelasticaltiger
Like Tigers in Coitus
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Re: Reusing a spore syringe? [Re: GoblinKing]
    #22239155 - 09/14/15 11:05 PM (8 years, 4 months ago)

Agar is a growing medium used to germinate spores in a petri diah or suitable substitute like a pasty plate.

Ditch the syringes is what everyone else meant to say.

Read pastywhites agar tek and the tiger drop for growing from your own spores.


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First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisibleelasticaltiger
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Re: Reusing a spore syringe? [Re: elasticaltiger]
    #22239176 - 09/14/15 11:17 PM (8 years, 4 months ago)

Your syringe is suspect. This is why it's important to test it on ahar aince youve already had two failures. What was your sterile teknique like? How did you inoculate the first two bags? Your entire procedure.

In order to get detailed replies we also need detailed information.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineQuit The Cult
World is yours

Registered: 11/12/14
Posts: 265
Last seen: 8 years, 3 months
Re: Reusing a spore syringe? [Re: elasticaltiger]
    #22244231 - 09/16/15 01:34 AM (8 years, 4 months ago)

I ordered my syringes from a shroomery sponsor,

I followed the instructions I was given

Made sure I worked in a still-air room. Sanitized the table and entire area I worked around including the walls. Even the bags themself.

Sprayed the hell out of the room with Lysol. Cleaned the inoculation point with 91% isopropyl rubbing alcohol immediately before inoculation.

Pulled out syringes. Flame sterilized with a bic lighter. After heating it up till the needle was red hot, I wiped a paper towel with rubbing alcohol along the red hot needle. Waited about ten seconds then inoculated.

Only pushed the needle into one area without pulling it back out to hit any other points in the bag to try and avoid worse chances of contams.

After pulling the needle out I put tape over the inoculation point and left the bags to sit.

Also I used gloves thru the whole process. Along with a dust mask.

Any tips are welcomed.

I have three syringes with enough spores to inoculate the new bags that will be coming anyday. It's fine to just Flame sterilize, squeeze a tad out, then resterilize and inoculate?


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Ill always have typos. Using a shitty phone to write on here.


Edited by cronicr (09/16/15 03:14 PM)


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Invisiblewowimflabbergasted
supercalifragilistic
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22244984 - 09/16/15 09:21 AM (8 years, 4 months ago)

Take the vendor name out of your post.

Don't wipe the needle with alcohol after you sterilize it, that is counter-productive.

70% ISO is better than 91% for mycology purposes.

That's all I can think of from what you said.


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Invisibledreadhed
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22245169 - 09/16/15 10:11 AM (8 years, 4 months ago)

Quote:

Quit The Cult said:
It's fine to just Flame sterilize, squeeze a tad out, then resterilize and inoculate?



Flame it, push the plunger a little bit to cool the needle, inoculate, repeat.


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OfflineKNOTHEAR
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Registered: 08/05/14
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Last seen: 6 years, 9 months
Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22246224 - 09/16/15 01:59 PM (8 years, 4 months ago)

Quote:

Quit The Cult said:


I followed the instructions I was given
Made sure I worked in a still-air room.




follow different instructions . do your work in a still-air BOX. you can't sanitize an entire room and you don't NEED to sanitize a box.you just need the air to be still.cost about $8 to make a SAB.


Edited by cronicr (09/16/15 03:15 PM)


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InvisibleInocuole
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Re: Reusing a spore syringe? [Re: wowimflabbergasted]
    #22246524 - 09/16/15 03:14 PM (8 years, 4 months ago)

Quote:

wowimflabbergasted said:
Take the vendor name out of your post.





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OfflineDankbud420
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Re: Reusing a spore syringe? [Re: Inocuole]
    #22246612 - 09/16/15 03:39 PM (8 years, 4 months ago)

I used my bathroom and cleaned it and sprayed the hell out of it with lysol too. I only got three out of 12 jars that didnt have mold. Needless to say I will be using a glove box next time and you should too lol


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OfflineQuit The Cult
World is yours

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Re: Reusing a spore syringe? [Re: Dankbud420]
    #22246662 - 09/16/15 03:51 PM (8 years, 4 months ago)

Thanks guys. And my bad I thought it was okay to talk about vendors if they were shroomery sponsors..? Either way a mod fixed my post. Gracias.


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OfflineKNOTHEAR
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Re: Reusing a spore syringe? [Re: Quit The Cult]
    #22250839 - 09/17/15 11:55 AM (8 years, 4 months ago)

don't use a glove box,in the long run they are dirtier than a SAB.


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