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Offlineuninc4life2010
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A few questions about cloning...
    #22235699 - 09/14/15 08:59 AM (8 years, 4 months ago)

I have a few mono's that are starting to pin, and I was considering cloning on agar for the first time.  I was wondering if it is necessary to wipe down the outside of the fruit with alcohol before cloning?  I know that you should rip the body open and take a sample of tissue from inside, but I'm not sure if it is important to clean the outside before placing it in the glovebox. 

Also, what is the strategy when choosing a fruit to clone?  Should I choose the largest singe fruit?  Should I choose a fruit from a cluster?  Should I look for the healthiest specimen, irrespective of size?  Any suggestions?


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Invisiblewowimflabbergasted
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Re: A few questions about cloning... [Re: uninc4life2010]
    #22235730 - 09/14/15 09:14 AM (8 years, 4 months ago)

No you don't need to sanitize the outside of the fruit. There was a whole thread on this a few months back I think. Most people clone from clusters or fast growing pins. It all depends on what traits you want to carry over to the next generation.


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OfflinePsilosopherr
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Re: A few questions about cloning... [Re: uninc4life2010]
    #22235760 - 09/14/15 09:26 AM (8 years, 4 months ago)

RR suggested dipping fruits in a bath of water that had been boiled for 20 minutes and then cooled. Iodine has also been suggested. (both of those are typically for cleaning up wild fruits, but why not be careful?)

For choosing clones personally I go for how straight a fruit is mostly. I don't want them too big or two small, no deformities


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Invisiblewowimflabbergasted
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Re: A few questions about cloning... [Re: Psilosopherr]
    #22235775 - 09/14/15 09:35 AM (8 years, 4 months ago)

I've never seen anybody dip a pin in boiled water before taking an inner tissue sample. The inside where you're taking the tissue from is already sterile there is no need to waste time on worrying about the outside.

Some people drop entire pins onto agar, but that's usually if the pins were growin invitro and haven't yet been exposed to open air.


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OfflinePsilosopherr
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Re: A few questions about cloning... [Re: wowimflabbergasted]
    #22235790 - 09/14/15 09:40 AM (8 years, 4 months ago)

is the OP just talking about cloning pins? I missed that


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Invisiblewowimflabbergasted
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Re: A few questions about cloning... [Re: Psilosopherr]
    #22235810 - 09/14/15 09:46 AM (8 years, 4 months ago)

I don't know I figured....he said his mono was starting to pin and it's best to take clones of pins so the myc is still vigorously growing.


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