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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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7+days no sign of Growth in jars
#22233608 - 09/13/15 07:22 PM (8 years, 4 months ago) |
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Used a MS syringe to innoc 10 jars (small) of: 50% horse shit/straw 50% coco coir /1% guano /1% gypsum -pasteurized
After 7+ days I see absolutely no signs of growth. I figured the syringe was bad. Used another syringe. 1cc into each. Been 4+ days no signs.
Is it going to take longer since its only 1cc or wtf
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22233867 - 09/13/15 08:12 PM (8 years, 4 months ago) |
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Well first of all link to the TEK you followed. 7 days is not a long enough time to worry 1cc is way more than enough Meth is bad If you pasteurized your jars you're fucked. You need to sterilize them.
Edited by Trusted cuItivator (09/13/15 08:18 PM)
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22233883 - 09/13/15 08:15 PM (8 years, 4 months ago) |
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Interesting mix. We're you going to fruit them as cakes?
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22233900 - 09/13/15 08:18 PM (8 years, 4 months ago) |
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Quote:
MajickMuffin said: After 7+ days I see absolutely no signs of growth. I figured the syringe was bad. Used another syringe. 1cc into each. Been 4+ days no signs.
Is it going to take longer since its only 1cc or wtf
Wait, hold up, you re-inoculated the same jars with another syringe? That'll just increase the chance of contams, being as MS syringes are never 100% sterile, and potentially throw off the moisture content of your jars.
And 1 week is not a long enough time frame to wait for MS to germinate. It sometimes takes up to two weeks to see growth. Next time make some agar plates, put spores on there, then make a nice LI syringe and inoculate with that.
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dowtish
biological disaster



Registered: 08/16/15
Posts: 150
Loc: Middle TN
Last seen: 6 years, 2 months
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Re: 7+days no sign of Growth in jars [Re: Psilosoulful]
#22233922 - 09/13/15 08:23 PM (8 years, 4 months ago) |
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That sounds like a kick ass substrate to mix colonized grains with but not to use for jars.....hmmmm....
-------------------- So crucify the ego, before it's far too late To leave behind this place so negative and blind and cynical, And you will come to find that we are all one mind Capable of all that's imagined and all conceivable. Just let the light touch you And let the words spill through And let them pass right through Bringing out our hope and reason ...

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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Re: 7+days no sign of Growth in jars [Re: dowtish]
#22234378 - 09/13/15 09:18 PM (8 years, 4 months ago) |
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Thanks for the input guys.
Yes I'm going to attempt to fruit them as cakes.
The horse shit/straw was already pasteurized, and then I added the coco coir, guano, gypsum and pasteurized them real well. Could have been between pasteurizing and sterilizing.
2cc total from MS into each jar, that won't throw off water content..
I doubt the MS are contaminated I've never had problems with them, also the substrate contents shouldn't be easy to contaminate. Although, I'm not sure since it is nutrient rich with guano powder and gypsum.
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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22234385 - 09/13/15 09:19 PM (8 years, 4 months ago) |
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I didn't follow any tek.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin] 1
#22235478 - 09/14/15 06:10 AM (8 years, 4 months ago) |
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Start over its pure fail.
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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Re: 7+days no sign of Growth in jars [Re: bodhisatta]
#22236400 - 09/14/15 12:26 PM (8 years, 4 months ago) |
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Quote:
bodhisatta said: Start over its pure fail.
Elaborate please
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megatacular
professor procrastinate


Registered: 08/18/15
Posts: 183
Loc: Shittown Texas
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22236422 - 09/14/15 12:32 PM (8 years, 4 months ago) |
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Agar man. Its the.only way. Then add a few wedges to grains in about 7 to 10 days add to bulk sub like you have there and put in trays or monotub. Success u will have with proper sterile tek
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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Re: 7+days no sign of Growth in jars [Re: megatacular]
#22236432 - 09/14/15 12:35 PM (8 years, 4 months ago) |
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Quote:
megatacular said: Agar man. Its the.only way. Then add a few wedges to grains in about 7 to 10 days add to bulk sub like you have there and put in trays or monotub. Success u will have with proper sterile tek
Why would you say 'its the only way' when its not. I've used MS so many times and hadsuccess
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megatacular
professor procrastinate


Registered: 08/18/15
Posts: 183
Loc: Shittown Texas
Last seen: 8 years, 4 months
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22236459 - 09/14/15 12:42 PM (8 years, 4 months ago) |
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Well its the only way to know for sure u have no contams. Good mycelial growth and the best chance to produce that great even blanket of the.same size and shape shroomage man. Its fool proof. We've all had our fare share of ms success. A few good clusters here and there but the best harvest always start with agar.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22236495 - 09/14/15 12:51 PM (8 years, 4 months ago) |
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Quote:
MajickMuffin said:
Quote:
bodhisatta said: Start over its pure fail.
Elaborate please
your trying to inoculate a bulk mix with spores, start with grain
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22236539 - 09/14/15 01:00 PM (8 years, 4 months ago) |
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Quote:
MajickMuffin said:
Quote:
bodhisatta said: Start over its pure fail.
Elaborate please
Try following before failing. Blaze your own trail when you know more than zilch
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megatacular
professor procrastinate


Registered: 08/18/15
Posts: 183
Loc: Shittown Texas
Last seen: 8 years, 4 months
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Re: 7+days no sign of Growth in jars [Re: cronicr]
#22236582 - 09/14/15 01:12 PM (8 years, 4 months ago) |
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Quote:
cronicr said:
Quote:
MajickMuffin said:
Quote:
bodhisatta said: Start over its pure fail.
Elaborate please
your trying to inoculate a bulk mix with spores, start with grain
Yeah listen to this guy I obviously don't.know shit. Don't.know why I bother sometimes. Good luck
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 7+days no sign of Growth in jars [Re: megatacular]
#22236611 - 09/14/15 01:19 PM (8 years, 4 months ago) |
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spores will rarely ever germinate on a bulk mix
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 7+days no sign of Growth in jars [Re: megatacular]
#22236612 - 09/14/15 01:19 PM (8 years, 4 months ago) |
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Quote:
megatacular said:
Quote:
cronicr said:
Quote:
MajickMuffin said:
Quote:
bodhisatta said: Start over its pure fail.
Elaborate please
your trying to inoculate a bulk mix with spores, start with grain
Yeah listen to this guy I obviously don't.know shit. Don't.know why I bother sometimes. Good luck
We all start somewhere
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
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Re: 7+days no sign of Growth in jars [Re: cronicr]
#22237172 - 09/14/15 03:28 PM (8 years, 4 months ago) |
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Quote:
cronicr said: spores will rarely ever germinate on a bulk mix
A bulk mix?
They should be able to start on the straw.
Ive done many bags of Horse Shit/straw and they start from spores just finely IME
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 7+days no sign of Growth in jars [Re: MajickMuffin]
#22237246 - 09/14/15 03:40 PM (8 years, 4 months ago) |
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pics? logs? links?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 7+days no sign of Growth in jars [Re: cronicr]
#22237254 - 09/14/15 03:42 PM (8 years, 4 months ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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