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VeganFungus
Stranger
Registered: 08/28/15
Posts: 3
Last seen: 7 years, 8 months
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Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?!
#22230568 - 09/13/15 09:10 AM (8 years, 4 months ago) |
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I'm truly trying to make since of it. I've had a few goes and realize how sterile you must be to succeed in this hobby. Mushrooms by their design obviously block contaminants for landing on the gills, but not at all the cap. Getting a cap from a monotub would be one thing, but how do people get prints from caps that have been in fanned sgfc's and open trays fruiting for weeks? The print and gills that you have placed on the jar/plate might be clean, but I don't see how the top of the clean-at-best mushroom cap could meet the fully sterile conditions required to not introduce contaminants to the jar your delicate spores will inhibit. What good does the SAB do if the cap hadn't been in one since birthed?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: VeganFungus]
#22230587 - 09/13/15 09:14 AM (8 years, 4 months ago) |
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Just do it.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: VeganFungus]
#22230612 - 09/13/15 09:22 AM (8 years, 4 months ago) |
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Quote:
VeganFungus said: I'm truly trying to make since of it. I've had a few goes and realize how sterile you must be to succeed in this hobby. Mushrooms by their design obviously block contaminants for landing on the gills, but not at all the cap. Getting a cap from a monotub would be one thing, but how do people get prints from caps that have been in fanned sgfc's and open trays fruiting for weeks? The print and gills that you have placed on the jar/plate might be clean, but I don't see how the top of the clean-at-best mushroom cap could meet the fully sterile conditions required to not introduce contaminants to the jar your delicate spores will inhibit. What good does the SAB do if the cap hadn't been in one since birthed?
Fruiting is done in open air, so the chances of getting an absolutely 100% clean print is slim to none. Just make sure everything else in the process is sterile....
When we use the spores, we take the minimum amount required for growth, so we have less of a chance of grabbing anything nasty.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 9 hours, 29 minutes
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: bodhisatta]
#22230617 - 09/13/15 09:23 AM (8 years, 4 months ago) |
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make a still air room take the sgfc in there right before the cap breaks the veil
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: tripdawg420]
#22230627 - 09/13/15 09:26 AM (8 years, 4 months ago) |
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There's going to be 1,000,000,000 spores per bacteria. If you use spores on a pf cake or something like that you have a extremely good chance of picking only spores. If you put spores into a LC even one bacterium will spoil the whole thing. On a cake if one bacterium gets in chances are it won't even form a small colony
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VeganFungus
Stranger
Registered: 08/28/15
Posts: 3
Last seen: 7 years, 8 months
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: PussyFart]
#22230662 - 09/13/15 09:35 AM (8 years, 4 months ago) |
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If you were making a spore print, how many 10cc syringes would you make? If you doubt it would be 100% clean, wouldn't it contaminate every thing you try to inoculate unless you separate the germs with agar? I understand clean is better than dirty, but I thought anything dealing with mycelium that isn't firmly established (colonized spawn) needs to be sterile not just clean? You can't inoculate with clean syringes, only sterile syringes. You pc to kill germs.... how is thia the one step in mycology that bypasses that?
Btw you're the trust cultivator, and even those who aren't who are giving input I greatly appreciate! I don't mean to come off as knowing it all, I have plenty to learn which is why I've been a lurker for nearly a year lol. I just simply know that "clean" usually isn't good enough when it comes to this hobby. I expected a tek saying to alcohol wipe the cap or something with it lol? I'm a but of an over thinker haha. Even if I made 20 prints that were successful I'd still wanna know how tf it's possible if open air inoculation isnt, for example
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: VeganFungus]
#22230728 - 09/13/15 09:52 AM (8 years, 4 months ago) |
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If you'd want to make a more clean print. Set up two foils per cap. Print in a SAB After an half hour to an hour take the cap and move it to the second foil. The first print will be good to use the second print can be assumed to be more clean as any endogenously provided contamination likely is left on print 1. Print 1 isn't unusable though.
If you have a 1 inch wide print that's pretty dark you can make 1-10+ 10cc syringes. A 2" wide print 50+ if you wanted.
Edited by Trusted cuItivator (09/13/15 09:52 AM)
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kiksen


Registered: 06/04/15
Posts: 60
Last seen: 2 months, 25 days
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: VeganFungus]
#22230731 - 09/13/15 09:53 AM (8 years, 4 months ago) |
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I have not failed a sporeprint yet, so just do it good luck!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: bodhisatta]
#22230733 - 09/13/15 09:54 AM (8 years, 4 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/21365919 I made this to show how small spores are. The tiny little smallest dot of spores you can see is uncountable
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: VeganFungus]
#22230796 - 09/13/15 10:06 AM (8 years, 4 months ago) |
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Quote:
VeganFungus said: If you were making a spore print, how many 10cc syringes would you make?
None.....I would go straight to agar....
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: PussyFart]
#22230833 - 09/13/15 10:12 AM (8 years, 4 months ago) |
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Quote:
PussyFart said:
Quote:
VeganFungus said: If you were making a spore print, how many 10cc syringes would you make?
None.....I would go straight to agar....
 This x200
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 9 hours, 29 minutes
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: bodhisatta]
#22230955 - 09/13/15 10:32 AM (8 years, 4 months ago) |
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,063
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Re: Would love help fully grasping how spore prints (not cleaned with agar) possibly succeed?! [Re: tripdawg420]
#22231078 - 09/13/15 11:00 AM (8 years, 4 months ago) |
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Keep in mind that people have been growing from spore syringes for over 25 years now in home 'labs' using only this method.
http://www.fanaticus.com/syringe.htm
The original instructions from 1991 high times.
I'm sure home growers back then had no idea about the level of sterility needed and I'm sure a lot of them had success. Otherwise the method would never have taken off.
Caps certainly aren't sterile but aluminum foil is pretty close. Printing on sterile foil makes it so that any contamination that falls with the prints won't have anything to propagate on ensuring that contaminating bacteria and mold spores on the print will stay right where they are. It also helps that mushroom spores on a typical print account for 99.9% of the spores on there. They win through pure numbers.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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