|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
megatacular
professor procrastinate


Registered: 08/18/15
Posts: 183
Loc: Shittown Texas
Last seen: 8 years, 4 months
|
Re: Breaking up spawn by hand [Re: sweetpea]
#22235299 - 09/14/15 02:42 AM (8 years, 4 months ago) |
|
|
Quote:
sweetpea said:
Quote:
megatacular said: Also how do you inoculate the jars? Ms syringe or???
Thisss, more details please. :o
If u must. Multi spore syringe is ones u but online for microscopy. Research that have multiple different genetics within. Another would be grain to grain. Simple one jar with colonized grains one with sterile grains. Take colonized grains add to uncolonized ones. Agar would be the best choice to innoc with because it would be free.of contams and provide the best and fastest growing fruits if isolated to a monoculture. Then Therese liquid culture grown in a jar in karo syrup or other media. Its the same asball other though never know what genetic ur gonna get till it grows. Agar is the best by far I think.
|
megatacular
professor procrastinate


Registered: 08/18/15
Posts: 183
Loc: Shittown Texas
Last seen: 8 years, 4 months
|
Re: Breaking up spawn by hand [Re: ShroominMe]
#22235301 - 09/14/15 02:45 AM (8 years, 4 months ago) |
|
|
Quote:
ShroominMe said: Too many variables here. What are you doing now that you did not do before?
Or, what did you do before, with success, that you are not doing now?
|
Spiracha
不拉差



Registered: 09/04/15
Posts: 52
Loc: distant galaxy
Last seen: 8 years, 3 months
|
Re: Breaking up spawn by hand [Re: Inocuole]
#22238066 - 09/14/15 06:48 PM (8 years, 4 months ago) |
|
|
Quote:
bodhisatta said: The contamination is In the jar if it contaminates before you see mushrooms.
bodhisatta is correct. This really isn't hard. If your monotubs are failing before fruiting any mushrooms, it is due to contams IN YOUR SPAWN.
Quote:
sweetpea said: the tub contaminating before you see mushrooms doesn't necessarily mean it came from the jars of spawn. Like, at all. In the OPs case, it sounds like a problem with mushy grains being compromised upon spawning. Even beyond that, there could be a problem with moisture content or pasteurization of the substrate that could prevent fruiting and eventually lead to contamination.
Which is like saying "its not your spawn, but it is caused by your spawn, maybe." Not trying to sound like a dick, it just is what it is. Also, if his grains are fully colonized with healthy myc then they would not fall victim to a contamination from spawning it to bulk. <---please correct me if im wrong here.
Quote:
sweetpea said: Also, he didn't say they turned a few days after spawning, he said after opening the tubs. I assume that to mean he was opening them after colonization with the intention of fruiting, but the compromised grain keeps eating shit at that point, having been unable to fully repair itself and succeptible to contams all the while. Just saying, no rudeness intended.
Usually the green shows once it has received fresh air. BEFORE getting fresh air trichoderma will appear white, like mycelium.
Just saying... still seems like bad spawn to me.
Quote:
Inocuole said: If it's coir it doesn't even need pasteurization.
Right. Coir is very contam resistant. If that is what OP used, then it makes the case of bad spawn an even stronger argument.
edit: idk how i screwed up the quotes there. fixed now
Edited by Spiracha (09/14/15 09:12 PM)
|
sweetpea

Registered: 12/29/13
Posts: 327
|
|
.
Edited by sweetpea (10/03/15 02:48 AM)
|
sweetpea

Registered: 12/29/13
Posts: 327
|
|
.
Edited by sweetpea (10/03/15 02:48 AM)
|
MushLover2552
Stranger

Registered: 08/13/15
Posts: 33
Last seen: 7 years, 6 months
|
Re: Breaking up spawn by hand [Re: sweetpea]
#22243344 - 09/15/15 08:50 PM (8 years, 4 months ago) |
|
|
If my WBS colonizes for a few days extra or if the jar is overfilled, I just wipe a knife down with iso and slice the jar up like a pizza, lid it, and shake til separated
|
blthirteenre



Registered: 06/10/12
Posts: 430
Loc: New York
Last seen: 9 months, 17 days
|
|
I want to be clear about what caused the failures and the possible reasons:
I used South American, orginally MS, G2G, and at this point, it was 4th generation.
In addition to verm and coir, I added coffee that had been frozen over a long period of time.
I definitely overfilled the jars to close to 800 ml including the grain transfer, which made it very difficult to get out of the jar.
A week or so ago, I made a tub with some Thai jars I had and it was successful.
Also, I still have some SA tubs that may be at its 4th, possibly 5th dunk that are still producing but possibly they are not related to the grain used for these failed tubs I am referring to.
I usually inject a pint jar and then G2G from there, and it is very possible that all these tubs that are failing is a result from the original pint jar I inoculated. I want to point out that every jar I have used for the tub not only looked perfect, but smelled perfect as well. The only issue was that it was impossible to get out of the jar so I had to rip it out one way or another.
The Thai I recently used was not overfilled and broke up just by shaking it.
I just bought some new syringes because the ones with SA and Thai are over a year old so I want to start over.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 1,697 topic views. 34 members, 232 guests and 34 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|