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aerow.thefox
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Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
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first no pour agar attempt
#22226735 - 09/12/15 11:40 AM (8 years, 4 months ago) |
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so iv tried agar before long ago with a pour method. i fucked it up hardcore and spilled agar all over my box and shit. needless to say it got contamed. but anyway i wanted to use the 1/4 pint jars(last time i had petris i just dont wanna order that stuff to my new place). i like the shape and i can reuse them for mini cakes later. my question to start is that iv read a couple people say that you can use agar in a closed container w/o a GE port, although most put one on. have any of you found this to be true? do you find better performance on agar with more GE? lemme know
btw im using an older PDA recipe. telephone brand agar potato starch water karo blue food coloring for better visuals
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Ge is not actually needed for a no pour jar. We typically have the ge hole to prevent a vacuum from forming and either crushing the container or making it super hard to get the lid off. Sometimes depending on the configuration of the lid and material its made of you can simply have it on loose in the PC and tighten it up when you pull it out. But if your working in a SAB you do nit want that lid to get sucked down and form a vacuum as it will potentially suck in air (and maybe contams) when you open it.
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aerow.thefox
professional amatuer



Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
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Re: first no pour agar attempt [Re: Pastywhyte]
#22227007 - 09/12/15 01:01 PM (8 years, 4 months ago) |
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pasty! just the person i was hopoing would comment.  thx for the info. also on a different note. i hadnt done this in a long time till recently and back then everybody still used glove boxes instead of SAB. so when i got my supplies i built a nice GB. iv read some of the supposed penefits but i just cant get past the idea of open arm holes. mine is a ziplock brand with a weather proof seal in the lid. pvc toilet mounts for arm holes. and dish gloves attatched to the inside of the box/PVCmounts. so what do use prefer? do you think my set up is good enough? would you still reccomend converting to a SAB instead?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I work in a SAB. Gloveboxes are typically not completely sealed. So your movements with the attached gloves actually pull in pockets of outside air and create turbulance. This is counterproductive. I also can't imagine sterilizing a dozen scalpels to do a round of transfers. You definitely don't want to be flaming tools inside a glovebox and so having the gloves unattached is way better IMO. Learn good movements in your SAB and you will enjoy a high rate of success. Also the agar will teach you if your movements are up to snuff.
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aerow.thefox
professional amatuer



Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
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Re: first no pour agar attempt [Re: Pastywhyte]
#22227158 - 09/12/15 01:41 PM (8 years, 4 months ago) |
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the only openings in my GB is in the lid. which the tub is flipped upside down so it has extra weights keeping the lid pressed tight and it has a weather proof seal. i doubt its perfect. but i feel like its really secure. but i definitely see you point about flame sterilization. i guess i'll just have to give it a whirl! im just working on a limited budget/space/spore supply. so im trying not to fuck it up so i can get a decent 'limited culture' and get some practice for taking a clone from it to optimize my grow for its small size.
Edited by aerow.thefox (09/12/15 01:42 PM)
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DampMushroom


Registered: 12/01/14
Posts: 115
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Re: first no pour agar attempt *DELETED* [Re: aerow.thefox]
#22227226 - 09/12/15 01:56 PM (8 years, 4 months ago) |
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Post deleted by DampMushroomReason for deletion: I've got to leave for a couple years. Hope the best for all of y'all.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Bacillus is more often than not indicative of improper sterilization. Never put a tissue biopsy to grain, thats why we have agar. Also shaking before 20% is way to early and will definitely lead to stalling.
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aerow.thefox
professional amatuer



Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
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Re: first no pour agar attempt [Re: Pastywhyte]
#22227358 - 09/12/15 02:32 PM (8 years, 4 months ago) |
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i was planning biopsy to agar.i hear fruits are not always isolates. when isolating from a fruit tissue biopsy, do you find it wise to still test the final, most vigorous sectors individually to find the best yielding? or will it vary so little as to be negligible?
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