Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineuninc4life2010
Unincorporated
Male User Gallery


Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost?
    #22225783 - 09/12/15 04:50 AM (8 years, 4 months ago)

My first time working with agar.  I placed a drop of spore syringe fluid onto each of the three sections of two petri dishes, and I have yet to see any growth?  How much longer should I wait before giving up hope?  BTW, I used PDA.  20 grams per 500 ml's of water. 


Edited by uninc4life2010 (09/12/15 04:52 AM)


Extras: Filter Print Post Top
OfflineLaughingcowwa
Your mum loves it.
Male


Registered: 05/02/15
Posts: 418
Last seen: 7 years, 9 months
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: uninc4life2010]
    #22225815 - 09/12/15 05:30 AM (8 years, 4 months ago)

You did what with 500mLs of water? You shouldn't be putting any fluids into your agar from what I know, if that's what you did there's your most likely answer


--------------------
Those who doubt me, suck cock by choice


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: Laughingcowwa]
    #22225837 - 09/12/15 05:49 AM (8 years, 4 months ago)

Quote:

Laughingcowwa said:
You did what with 500mLs of water? You shouldn't be putting any fluids into your agar from what I know, if that's what you did there's your most likely answer



Agar is made of like >90% water unless the recipe is super stiff. (insert boner joke here)

3 weeks is way too long for spores on agar.  Try again. :shrug:

Maybe sterilize the plug and then put it back on the syringe and do the trick with the rubber bands or whatever where you apply pressure to the spores to force-hydrate them in case that's the issue.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Offlineuninc4life2010
Unincorporated
Male User Gallery


Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: Inocuole]
    #22225864 - 09/12/15 06:14 AM (8 years, 4 months ago)

Okay.  The only other thing I can think of is that the agar wasn't completely cool when the spores were dropped on.  That could've caused them to die and never germinate.  Besides that, I have no clue why there isn't any growth.


Extras: Filter Print Post Top
InvisibleMr.GuessWork
Stranger
 User Gallery
Registered: 03/30/13
Posts: 4,563
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: uninc4life2010]
    #22225876 - 09/12/15 06:24 AM (8 years, 4 months ago)

Did you shake the syringe up really good before putting the drops on? I'm not sure what the average spore density is in a syringe, but if it's really low, then a drop might not be enough fluid, especially if the spores are all clumped up in one spot. I've had it happen too with some shitty spore syringes I made myself. I prefer prints now.


Extras: Filter Print Post Top
OfflineNature Boy
Stranger than most
Male User Gallery


Registered: 07/09/07
Posts: 8,241
Loc: Samsara
Last seen: 2 months, 6 days
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: uninc4life2010]
    #22226063 - 09/12/15 08:04 AM (8 years, 4 months ago)

Wow...this thread is so full of fail it is hysterical.  Your agar formula a little too nutritious, but should still work FINE.  Nothing wrong with 20g PDA and 10g agar in 500ml.  That's above the standard 2%, though.  Ignore the joker, above.  As has been suggested, shaking the shit out of the syringe is a prerequisite to getting them suspended.  That is excellent advice.  Next time drop the first few drops into a sink or napkin, that way you'll be guaranteed that the first and only drop (or two) that goes onto your agar will actually contain spores.

The good news is that your petri's are good and sterile if they haven't contaminated in 3 weeks.  Good job on that!  Just go ahead and try again now that the agar is room temperature!  :lol:

N.B.


--------------------
All submitted posts under this user name are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit.  Note well:  Sorry, but I do not answer PM's unless you are a long-time trusted friend.  If you have a question, ask it in the appropriate thread.

                                                                               


Extras: Filter Print Post Top
Offlineuninc4life2010
Unincorporated
Male User Gallery


Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: Nature Boy]
    #22227015 - 09/12/15 01:04 PM (8 years, 4 months ago)

Quote:

Nature Boy said:
Wow...this thread is so full of fail it is hysterical.  Your agar formula a little too nutritious, but should still work FINE.  Nothing wrong with 20g PDA and 10g agar in 500ml.  That's above the standard 2%, though.  Ignore the joker, above.  As has been suggested, shaking the shit out of the syringe is a prerequisite to getting them suspended.  That is excellent advice.  Next time drop the first few drops into a sink or napkin, that way you'll be guaranteed that the first and only drop (or two) that goes onto your agar will actually contain spores.

The good news is that your petri's are good and sterile if they haven't contaminated in 3 weeks.  Good job on that!  Just go ahead and try again now that the agar is room temperature!  :lol:

N.B.




Wait do I need to add anything to my PDA?  I just spooned in 20 grams as per the instructions, added half a liter of water, and pressure cooked the bottle.  In the future, I'm waiting until it cools off a little more before pouring, since all of my plates got serious condensation on them. 

I'll make sure to shake the syringe a little more next time.  Who knows why they never germinated. :shrug:


Extras: Filter Print Post Top
Invisiblejbaby007
Badass
I'm a teapot


Registered: 02/28/15
Posts: 1,026
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: uninc4life2010]
    #22227133 - 09/12/15 01:35 PM (8 years, 4 months ago)

Definitely allow your mix to cool down before adding your spores. I follow Pasty's Easy Agar Tek and besides the agar and water, it says to add a drop of honey or corn syrup. It also mentions potato flakes. It's the only tek I followed but there are other agar teks you can follow. This one just works for me.


--------------------


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: jbaby007]
    #22227202 - 09/12/15 01:50 PM (8 years, 4 months ago)

Quote:

Wait do I need to add anything to my PDA?  I just spooned in 20 grams as per the instructions, added half a liter of water, and pressure cooked the bottle.  In the future, I'm waiting until it cools off a little more before pouring, since all of my plates got serious condensation on them. 




after my PC is done, and reached zero, i wait an hour to open it.  take out, wait another half hour-45 minutes before pouring.  u should be able to easily hold it in ur hand with no protection before pouring.  it will limit ur condensation ALOT.

in the winter, after its solidifies, put into gallon bags, and leave them near a space heater, and that will help get rid of the rest.

3 weeks is a long time.  i would swipe again.

u can also pour warm agar onto spores, thats how i germinate because i use stiff agar (awesome for xferring/cloning, but just sucks for germination).  use the last few plates to do this (agar is even cooler at this point).  squirt/scrape ur spores onto the plate, then pour on.  the warm agar will hug the spores and soften them, making germination easier.  more often than not, the spores will germinate along the sides of the plate, because the current of the poured agar has brought them there



--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
OfflineCubensisFrenzy
Male

Registered: 07/15/15
Posts: 27
Last seen: 8 years, 3 months
Re: It's been 3 weeks since I dropped spores onto my agar plates. Is all hope lost? [Re: Nature Boy]
    #22227209 - 09/12/15 01:52 PM (8 years, 4 months ago)

Quote:

Nature Boy said:
Wow...this thread is so full of fail it is hysterical.  Your agar formula a little too nutritious, but should still work FINE.  Nothing wrong with 20g PDA and 10g agar in 500ml.  That's above the standard 2%, though.  Ignore the joker, above.  As has been suggested, shaking the shit out of the syringe is a prerequisite to getting them suspended.  That is excellent advice.  Next time drop the first few drops into a sink or napkin, that way you'll be guaranteed that the first and only drop (or two) that goes onto your agar will actually contain spores.
The good news is that your petri's are good and sterile if they haven't contaminated in 3 weeks.  Good job on that!  Just go ahead and try again now that the agar is room temperature!  :lol:

N.B.



+1

As long as your sure about your agar mix, then just give it another go with new spores... Your dishes are clean so, just use the same ones. I'd recommend not using the same syringe again. If you have the option, get some different spores or clone tissue.
If not, cool the needle first and maybe use 2-3 drops if you think the syringe may lack enough spores.  Just hold it with the needle pointing to the floor for a little while and let gravity bring the spores toward the needle.

Just try not to move the dishes around afterward or the solution will slide around the surface leaving you with spores germinating all over the dish.

Just watch the damn video.....


Edited by CubensisFrenzy (09/12/15 02:06 PM)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Whats with my agar plates? Frankenstein 8,600 8 09/08/22 03:46 PM
by schpat
* Re: mold in agar plate Anonymous 12,189 3 12/04/99 12:35 AM
by Anonymous
* dropping spores early flpntwst 1,329 4 07/19/01 06:18 PM
by flpntwst
* How many mycelium syringes from one agar plate? BBShrizz 4,883 3 05/10/01 02:10 AM
by ISaToriBluEI
* dropped spores, yikes! pl151 1,515 2 05/31/01 02:19 PM
by jonnyshaggs420
* Re: agar plate question Psilowarrior 2,541 2 12/10/99 02:09 PM
by rebelmoon
* Can I innoculate agar plate with a syringe? Numba9 2,093 4 09/01/01 02:19 PM
by ralphster44
* Dropping spores... a quick question dopamine 997 1 02/15/02 02:17 PM
by MickyFinn

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
748 topic views. 35 members, 264 guests and 51 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.01 seconds on 14 queries.