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OfflineTrippieHunter
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Registered: 04/05/15
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Does this agar look right?
    #22224430 - 09/11/15 09:40 PM (8 years, 4 months ago)




My first go using the pastywhytes method. Swabbed from spore print. Used 2 different prints and 6 out of 8 dishes look like this, no not 2 different per container. I may have gone a little over board on some of the agar with the swab. Please let me know. Thanks!


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Edited by TrippieHunter (09/11/15 09:42 PM)


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OfflineSpiracha
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Re: Does this agar look right? [Re: TrippieHunter]
    #22224449 - 09/11/15 09:43 PM (8 years, 4 months ago)

Looks like it STINKS!
My first agar looked like that. No bueno.

Wait for another opinion tho.


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OfflineTrippieHunter
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Re: Does this agar look right? [Re: Spiracha]
    #22224455 - 09/11/15 09:45 PM (8 years, 4 months ago)

I stored in my house at like 70*, where would/could I have gone wrong?


--------------------
Just remember keep the camera rolling and
FILM THE POLICE!!! CLICK ME

WHO'S SIDE ARE YOU ON? CLICK ME TOO!

Let it go and you will trip into wonderland!


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OfflineSpiracha
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Re: Does this agar look right? [Re: TrippieHunter]
    #22224472 - 09/11/15 09:49 PM (8 years, 4 months ago)

With me, I had bad sterile procedure at the time (didn't take it as serious as I shoulda). For you, who knows. I would wait tho for someone else to give their opinion. I haven't been doing agar too long. But I do know what healthy growth looks like and that aint it.


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OfflineTrippieHunter
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Re: Does this agar look right? [Re: Spiracha]
    #22224478 - 09/11/15 09:50 PM (8 years, 4 months ago)

I wonder what the temp limits/tolerances are that the agar should be in? Sorry for the newb question.


--------------------
Just remember keep the camera rolling and
FILM THE POLICE!!! CLICK ME

WHO'S SIDE ARE YOU ON? CLICK ME TOO!

Let it go and you will trip into wonderland!


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Invisibleazur
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Re: Does this agar look right? [Re: TrippieHunter]
    #22224483 - 09/11/15 09:50 PM (8 years, 4 months ago)

Idk. Nasty bacteria maybe?  But tthat thing is fucked


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OfflineSpiracha
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Re: Does this agar look right? [Re: TrippieHunter]
    #22224493 - 09/11/15 09:53 PM (8 years, 4 months ago)

Storing at room temp is fine.


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OfflineTrippieHunter
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Re: Does this agar look right? [Re: azur]
    #22224501 - 09/11/15 09:53 PM (8 years, 4 months ago)

Is it possible the micropore tape I used wasn't so porous that it couldn't breath? I made 1 quarter inch hole and used what I was told comparable micropore tape.


--------------------
Just remember keep the camera rolling and
FILM THE POLICE!!! CLICK ME

WHO'S SIDE ARE YOU ON? CLICK ME TOO!

Let it go and you will trip into wonderland!


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InvisibleLocN9ne
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Re: Does this agar look right? [Re: TrippieHunter]
    #22224511 - 09/11/15 09:55 PM (8 years, 4 months ago)

My thoughts are,Too much water pooling/rolling around on top...try letting it cool a little more before you pour it into the dishes to help cut down on condensation... just my uneducated guess.


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OfflineSpiracha
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Re: Does this agar look right? [Re: TrippieHunter]
    #22224523 - 09/11/15 09:57 PM (8 years, 4 months ago)

No. I wouldn't think so. The 1/4 inch hole in the lid that is taped over with micropore tape is mainly there so the pressure cooker doesnt crush the mini rounds. It wasnt a design for GE.


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Invisibleazur
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Re: Does this agar look right? [Re: TrippieHunter]
    #22224530 - 09/11/15 09:58 PM (8 years, 4 months ago)

Idk about the tape. I pour my plates. But your swabs were covered in bacteria. Trash it.
Next time i suggest making a syringe and putting 1 drop on the plate.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineSpiracha
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Re: Does this agar look right? [Re: TrippieHunter]
    #22224537 - 09/11/15 10:00 PM (8 years, 4 months ago)

I see agar slopped all up the walls of that mini round, covered with that bacteria oozing down... What up with that?


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OfflineiSmkGrnBud
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Re: Does this agar look right? [Re: Spiracha]
    #22224571 - 09/11/15 10:06 PM (8 years, 4 months ago)

Ditch it and try again. If you're gonna try from a print again, try a loop instead of a swab?


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OfflinecronicrFacebook
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Re: Does this agar look right? [Re: iSmkGrnBud]
    #22224758 - 09/11/15 10:38 PM (8 years, 4 months ago)

yep that's bacteria all right, try again


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Invisiblestareatclouds
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Re: Does this agar look right? [Re: cronicr]
    #22225700 - 09/12/15 03:39 AM (8 years, 4 months ago)

Oh yeah, 100% bacteria, but that's normal when you're first getting started. In fact, here's my first plate last November. Looks familiar, eh? :smile:



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OfflineTrippieHunter
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Re: Does this agar look right? [Re: stareatclouds]
    #22226322 - 09/12/15 09:29 AM (8 years, 4 months ago)

Fucking a, thanks guys. I was pretty damn careful with everything. I will take all of your advice and tips. Thanks guys.


--------------------
Just remember keep the camera rolling and
FILM THE POLICE!!! CLICK ME

WHO'S SIDE ARE YOU ON? CLICK ME TOO!

Let it go and you will trip into wonderland!


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InvisibleMad Season
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Re: Does this agar look right? [Re: TrippieHunter]
    #22226338 - 09/12/15 09:34 AM (8 years, 4 months ago)

Did you use just 1 drop of spores? Also when doing spores i like to use 2 or 3 dishes in case 1 of them does go bacterial.


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InvisibleInocuole
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Re: Does this agar look right? [Re: Mad Season]
    #22226548 - 09/12/15 10:40 AM (8 years, 4 months ago)

I've NEVER had bacteria.  You guys are noobs.



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OfflineTrippieHunter
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Re: Does this agar look right? [Re: Inocuole]
    #22226699 - 09/12/15 11:28 AM (8 years, 4 months ago)

Quote:

Inocuole said:
I've NEVER had bacteria.  You guys are noobs.







You are right I am a noob, that's why I am asking these questions. Thanks for the insight though it has contributed to my thread.


--------------------
Just remember keep the camera rolling and
FILM THE POLICE!!! CLICK ME

WHO'S SIDE ARE YOU ON? CLICK ME TOO!

Let it go and you will trip into wonderland!


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InvisibleInocuole
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Re: Does this agar look right? [Re: TrippieHunter]
    #22226705 - 09/12/15 11:30 AM (8 years, 4 months ago)

I like how you purposefully edited out the punchline of my post basically and made it seem like I was being serious.  Good stuff there.

Was just confirming that we've all had that particular thing happen for you.


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