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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: contamination pic of the day [Re: Huskies]
#23786928 - 10/30/16 09:12 PM (7 years, 2 months ago) |
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When the bug hits that's the time to scratch it
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Huskies
Boop More Snoots



Registered: 03/22/16
Posts: 1,048
Last seen: 21 hours, 8 minutes
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Re: contamination pic of the day [Re: azur]
#23788024 - 10/31/16 10:01 AM (7 years, 2 months ago) |
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Yup. By the way, is condensation out of nowhere a good sign of contams in grain jars? I have one that is definitely moldy (its out for disposal now) and it has condensation around that area. The jar I posted which was possibly not also started to sweat.
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: contamination pic of the day [Re: Huskies]
#23788035 - 10/31/16 10:05 AM (7 years, 2 months ago) |
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Condensation is a sign of temp differences. Could mean it got cold last night. Could mean something bad is heating things up. Could mean nothing
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: contamination pic of the day [Re: azur]
#23788043 - 10/31/16 10:07 AM (7 years, 2 months ago) |
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My jars get condensation whether they are good or bad but ive noticed when they are not almost all the way colonized with moisture tgey go bacterial but not every time just most of the time just keep an eye on them ... do you use agar i havent read that or remembered seeing it haha
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Huskies
Boop More Snoots



Registered: 03/22/16
Posts: 1,048
Last seen: 21 hours, 8 minutes
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Re: contamination pic of the day [Re: Boogieman47]
#23788061 - 10/31/16 10:11 AM (7 years, 2 months ago) |
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Yeah I have begun using agar, the jar I posted was from an agar plate. Let's hope the rest of that batch comes out fine. That being said it was a lets try to save this left over rye berries that pantry moths have infested, so if they all contam I guess it was a valiant (or retarded) effort haha
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: contamination pic of the day [Re: Huskies]
#23788122 - 10/31/16 10:32 AM (7 years, 2 months ago) |
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Well even with agar you can still get a piggy back contam it may look clean but isnt if you transfer at about the size of a penny and take small rice grain transfer 3 to 4 times then you should have a definite clean plate and even then not always will be clean ... if the rye was pc'd after that they should of been good ive pc'd oats that i let sit till white fuzz grew caus eim lazy and they worked fine a little bacterial but still got flushes
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Tryptallmine
Stranger
Registered: 10/29/16
Posts: 4
Last seen: 7 years, 2 months
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Quote:
PinPornProducer said: Im assuming you are making syringes from a print? Either you have a very dirty print or your sterile technique needs improvement. Are you scraping spores into a iso cleaned shot glass? What's your process of getting spores into the syringe?
It was definitely the syringes. All the mold accumulated right at the injection points.
This time I cut a corner and took small new snaplock bag, put what was left of the print in there and syringed 40ml of clean water in there. Shook it around and left the bag in the fridge for ~3 days. Then drew that up with a long drawing needle out of the bag (didn't need to open the bag) only enough to get the drawing needle in. Wiped down the needle with alcohol. No flame sterilization. Lesson learned I guess.
Although the mushroom gods seem to be on my side. I have 1 jar out of 18 that is growing healthy with no sign of contamination. This is doing to be my last ditch @ growing this year as I have no more spores.
Do you think I should cut some slices off this good cake and inoculate 6 more cakes in a SAB? What's going to be the best way to make this 1 cake go the distance?
Edited by Tryptallmine (11/05/16 02:15 PM)
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: contamination pic of the day [Re: Tryptallmine]
#23803577 - 11/05/16 02:39 PM (7 years, 2 months ago) |
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Not quite sure, never tried to expand a cake. Uh... If it were me and I wanted to do it I guess I would take a sterile syringe and long needle full of sterilized water. Flame the needle, insert it into the the injection ports of the colonized cake and take a plug out of each injection hole of the cake and shoot plugs into the new jars. I mean I guess you could possibly in a sab scrape back the verm layers and drop slices into the new jars but this is uncharted territory for me. Let's just hope that you in fact do have a good cake and get some fruits you can print.
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Tryptallmine
Stranger
Registered: 10/29/16
Posts: 4
Last seen: 7 years, 2 months
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Quote:
PinPornProducer said: Not quite sure, never tried to expand a cake. Uh... If it were me and I wanted to do it I guess I would take a sterile syringe and long needle full of sterilized water. Flame the needle, insert it into the the injection ports of the colonized cake and take a plug out of each injection hole of the cake and shoot plugs into the new jars. I mean I guess you could possibly in a sab scrape back the verm layers and drop slices into the new jars but this is uncharted territory for me. Let's just hope that you in fact do have a good cake and get some fruits you can print.
Yeah...I have one other jar that's almost fully colonized. It does have 2 tiny spots of Trich on it.
I'm going to cut some slices off that cake as the majority of the cake is healthy. That way I can at a least have a go at getting a few cakes grown to get some healthy prints. If they all get contaminated then at least I tried.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: contamination pic of the day [Re: Tryptallmine]
#23803846 - 11/05/16 04:22 PM (7 years, 2 months ago) |
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If there is green showing then the entire cake is mold ridden. I wouldn't mess with it tbh. You can try fruiting it in an isolated chamber but chances are it's just going to go green before fruiting. I wouldn't waste my time trying to expand it. Most molds are white before sporulatung and turning green. Guarunteed mold is throughout most of that cake
Edited by PinPornProducer (11/05/16 04:23 PM)
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Learn agar and take a chunk of that colonized cake to agar, clean it uo and knock up some jars with it. That would be your best bet at keeping what you got and having the highest chances of success without buying more spores and starting over
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Tryptallmine
Stranger
Registered: 10/29/16
Posts: 4
Last seen: 7 years, 2 months
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Re: contamination pic of the day [Re: natedawgnow]
#23804955 - 11/05/16 10:02 PM (7 years, 2 months ago) |
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Thanks for the help guys. If you can point me in the direction of the Agar teks, that'd be great. I've got plenty of Petri dishes laying around. I've been struggling to find tek info, it'll just take time to find my feet here.
So I found another Cubensis syringe in the fridge hiding from the first spore print. I've inoculated 6 fresh BRF cakes. I decided to cut a slice of clean looking mycelium off the slightly contaminated cake and threw it into a 7th cake just for interest sake. I'll see how it goes!
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: contamination pic of the day [Re: Tryptallmine]
#23806421 - 11/06/16 12:37 PM (7 years, 2 months ago) |
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anyone else every get purple contams? This jar was gnarly
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: contamination pic of the day [Re: Boogieman47]
#23806435 - 11/06/16 12:38 PM (7 years, 2 months ago) |
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That shits no joke, I think that purple shit causes spinal meningitis if I remember reading correctly
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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That would br fucked to pass that on man
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: contamination pic of the day [Re: Boogieman47]
#23808550 - 11/07/16 03:53 AM (7 years, 2 months ago) |
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one time at band camp I did get purple con tams

-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: contamination pic of the day [Re: blindingleaf]
#23808762 - 11/07/16 07:11 AM (7 years, 2 months ago) |
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: contamination pic of the day [Re: Pastywhyte]
#23808853 - 11/07/16 08:19 AM (7 years, 2 months ago) |
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Shits fucking gnarly looking
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tukkis
CEO


Registered: 12/24/13
Posts: 68
Last seen: 2 years, 10 months
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Quote:
PinPornProducer said: That shits no joke, I think that purple shit causes spinal meningitis if I remember reading correctly
seriously? that's fucked. i'm going to be extra careful with my contams now and pressure cook EVERYTHING before tossing out. not like i wasn't careful before but...
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: contamination pic of the day [Re: tukkis]
#23809025 - 11/07/16 09:24 AM (7 years, 2 months ago) |
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No need to get all crazy over fusarium mold mycotoxins. Much like all other mycotoxins, they won't harm you unless you have a weakened immune system/lots of exposure. If you're healthy, and toss your molds like good people, you won't have any problems because almost every mold in this hobby has mycotoxins.
Aspergillus, penicillium, trichoderma, fusarium, lipstick, etc. all possess mycotoxins anyone with a weakened immune system should be wary of.
http://www.mold-help.org/content/view/457/ ^ list of mycotoxins and the species of each. You'd be shocked how many genuses y'all should recognize
Edited by Mad Season (11/07/16 11:17 AM)
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