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OfflineThe Mycologist
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Registered: 05/06/16
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Re: contamination pic of the day [Re: NumeroEno]
    #23678119 - 09/25/16 09:43 PM (7 years, 4 months ago)

:breakout:


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflinePinPornProducer
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Registered: 08/23/14
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Re: contamination pic of the day [Re: NumeroEno]
    #23679949 - 09/26/16 01:58 PM (7 years, 4 months ago)

Quote:

NumeroEno said:
Aspergillus niger growing on an uninoculated plate :deathstar:





This is not good


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InvisibleNumeroEno
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Registered: 07/24/14
Posts: 9,652
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Re: contamination pic of the day [Re: PinPornProducer]
    #23679982 - 09/26/16 02:12 PM (7 years, 4 months ago)

1 out of 40 plates that I poured contaminated. I'm not too worried about it. If that shit gets into a grain jar I'll see it too. IMO as long as those contams don't get spawned to bulk or released into the air they're harmless.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineSmoothcat
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Re: contamination pic of the day [Re: NumeroEno]
    #23680055 - 09/26/16 02:36 PM (7 years, 4 months ago)

Hi first time in this thread :hi:

Contaminated agar plate, I get the green is contam, but how about the pale stuff on the edge of the plate? Contam or streaky agar??

Just thought I'd share with you anyway and any comments welcome :thumbup:

   


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Offlinemrmazdarx9
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Registered: 05/15/16
Posts: 9,796
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Re: contamination pic of the day [Re: Smoothcat]
    #23680057 - 09/26/16 02:37 PM (7 years, 4 months ago)

Bacteria


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If you use "SWIM" you should DROWN


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OfflineSmoothcat
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Re: contamination pic of the day [Re: mrmazdarx9]
    #23680066 - 09/26/16 02:40 PM (7 years, 4 months ago)

Hmmm I wonder if that's come from my fridge then where I stored my poured plates.... All my other plates have been fine


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Invisibledankington
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Re: contamination pic of the day [Re: Smoothcat]
    #23680075 - 09/26/16 02:44 PM (7 years, 4 months ago)

Are you wrapping your plates? Because it looks in other photos like you just hold them shut with micropore. If that's the case, you should at least try cutting a 1" roll off of a roll of saran wrap (thanks for the idea, Bodhi). You should always wrap your petri dishes after transfer/inoculation.


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OfflineSmoothcat
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Re: contamination pic of the day [Re: dankington]
    #23680096 - 09/26/16 02:49 PM (7 years, 4 months ago)

Cheers danks :thumbup:

Yeah the first few plates I only used micro pore but they went bad so I started wrapping them tight in cling film now. Better success with that but I put about 10 petri's in the fridge not individually wrapped but tied up in the sleeve they came in. I think that could be the problem as the wrapped ones seem to fare better :shrug:


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InvisibleNumeroEno
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Registered: 07/24/14
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Re: contamination pic of the day [Re: Smoothcat]
    #23680100 - 09/26/16 02:52 PM (7 years, 4 months ago)

It's worth it to order a big ass roll of parafilm. I love the stuff.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineSmoothcat
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Re: contamination pic of the day [Re: NumeroEno]
    #23680170 - 09/26/16 03:10 PM (7 years, 4 months ago)

Haha thanks for the encouragement!!

Just ordered two rolls off auction site eBay, is 5cm wide enough?


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InvisibleNumeroEno
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Re: contamination pic of the day [Re: Smoothcat]
    #23680631 - 09/26/16 05:59 PM (7 years, 4 months ago)

Yeah. That's plenty wide. I use the 2" stuff and cut it down the middle.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleBoogieman47
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Registered: 03/05/16
Posts: 9,712
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Re: contamination pic of the day [Re: NumeroEno]
    #23680698 - 09/26/16 06:19 PM (7 years, 4 months ago)

didnt have the jar on tight when i shook it thought it might of been ok haha


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OfflineGreg
always learning
I'm a teapot


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Re: contamination pic of the day [Re: Boogieman47]
    #23680792 - 09/26/16 06:41 PM (7 years, 4 months ago)

Quote:

noob47 said:
didnt have the jar on tight when i shook it thought it might of been ok haha



I got the same damn mold (penicillium sp.?) after I shook a Reishi jar. RIP in peace.


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InvisibleBoogieman47
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Re: contamination pic of the day [Re: Greg]
    #23680806 - 09/26/16 06:44 PM (7 years, 4 months ago)

Whatever it is i dont like it haha


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OfflineThe Mycologist
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Registered: 05/06/16
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Re: contamination pic of the day [Re: Boogieman47]
    #23684232 - 09/27/16 07:11 PM (7 years, 4 months ago)

I just looked up parafilm on amazon, its pretty expensive.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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InvisibleNumeroEno
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Registered: 07/24/14
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Re: contamination pic of the day [Re: The Mycologist]
    #23684243 - 09/27/16 07:14 PM (7 years, 4 months ago)

Shroomsupply.com has a good deal on it, and so does cynmar if you order a bunch of petris to hit their minimum. The smaller the amount you get, the more you'll pay for foot. I bought a 250' roll well over a year ago and still have lots left.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineSmoothcat
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Registered: 10/07/15
Posts: 1,254
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Re: contamination pic of the day [Re: NumeroEno]
    #23684249 - 09/27/16 07:16 PM (7 years, 4 months ago)

£2.50 on eBay....


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InvisibleNumeroEno
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Registered: 07/24/14
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Re: contamination pic of the day [Re: Smoothcat]
    #23684279 - 09/27/16 07:21 PM (7 years, 4 months ago)

Don't know for how much, but you should be able to find a 250 foot roll for under $30. £2.50 is what, like $4?


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 3 hours
Re: contamination pic of the day [Re: NumeroEno]
    #23684994 - 09/27/16 11:44 PM (7 years, 4 months ago)

Thanks guys found a nice deal.:leocheers:


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Invisibledankington
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Re: contamination pic of the day [Re: The Mycologist]
    #23689879 - 09/29/16 09:42 AM (7 years, 3 months ago)

I fucking hate Mycogone. Saw a white blob on my PE tub, but knew it wasn't a blob. It then started getting a couple of brown spots, then later started that yellow perspiration bullshit. So I took it out of the room and stuck it in the window of another room.

   

Here's the culprit. Like, wtf dude?

   

That's a first flush. Not too shabby, 2qts in a mini. Still working on dialing in micropore tape.


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