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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
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Re: contamination pic of the day [Re: azur]
#23119833 - 04/15/16 07:41 AM (7 years, 9 months ago) |
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-------------------- AMU Q&A
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: contamination pic of the day [Re: SloppyJoseph]
#23119854 - 04/15/16 07:54 AM (7 years, 9 months ago) |
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Quote:
SloppyJoseph said: That would be cool munch, thank you. Resurrect the tampfest thread with the pics because I'm pretty sure I'm not the only one who was confused by the look of the mycelium.
I threw out countless pan cyan germination plates because I had never seen the myc before... Come to find out that it also doesn't really look like cube myc...
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Q&A US vs. THEM The more I learn, the less I know.
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betaWP
PE, Chitwan, AA+, ATL7



Registered: 02/24/15
Posts: 31
Last seen: 4 years, 8 months
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Re: contamination pic of the day [Re: LocN9ne]
#23148407 - 04/23/16 08:51 PM (7 years, 9 months ago) |
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Larva crashes the party, courtesy of reddit user EggYoch.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: contamination pic of the day [Re: betaWP] 1
#23148462 - 04/23/16 09:12 PM (7 years, 9 months ago) |
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Hmm, offsite link. To click, or not to click?
No, thanks.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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I clicked heh. Just some larvae in a jar. =p
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: contamination pic of the day [Re: mupetmower]
#23148479 - 04/23/16 09:17 PM (7 years, 9 months ago) |
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Q&A US vs. THEM The more I learn, the less I know.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: contamination pic of the day [Re: LocN9ne]
#23148488 - 04/23/16 09:21 PM (7 years, 9 months ago) |
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Hey loc, do you have any experience with meshed in molds?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: contamination pic of the day [Re: LocN9ne]
#23148490 - 04/23/16 09:23 PM (7 years, 9 months ago) |
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Quote:
LocN9ne said:

Reminds me of the last time I got hammered and did a marathon in the SAB. Finished up thing fuck I am a boss. Three days later
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Quote:
Supalemonhaze said: Hey loc, do you have any experience with meshed in molds?
Not really that I'm aware of... Its usually "meshed" in bacteria that I have to deal with... The molds I get are usually just like that picture because I get sloppy as Fuck in the SAB... If I'm doing more than like 30 transfers I need a fucking cigarette so bad by the time I'm nearing the end that I just Fuck it all up...
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Q&A US vs. THEM The more I learn, the less I know.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: contamination pic of the day [Re: LocN9ne]
#23149624 - 04/24/16 08:58 AM (7 years, 9 months ago) |
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That happens to me as well only I try my best not to fuck up. God knows I get plenty of those.
Okay, no worries. If anyone does know a bit about them, any and all help on this thread is much appreciated.
I was also curious if a meshed in mold ever sectors away from your shroom mycelium or if they are as good as trash. I just threw a shitload of cultures that I suspected of having meshed mold this morning.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Meshed in molds are best handled with a hot pour IMO. I heard of people using antibiotics or pH buffering but I never tried them myself.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: contamination pic of the day [Re: Pastywhyte]
#23149704 - 04/24/16 09:40 AM (7 years, 9 months ago) |
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They die with a hot pour? Didn't know that.
Can you take a look at that thread I linked to see if you think I have any?
I ain't exactly sure bout it but sometimes it seems like the cultures are having a hard time staying above the surface of the agar only the media is nice and hard. Others start to develop a bit of rhizo growth only to turn cottony all of a sudden or they get these wierd sectors where the mycelium seems a lot thinner.
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Re: contamination pic of the day [Re: LocN9ne]
#23150020 - 04/24/16 12:08 PM (7 years, 9 months ago) |
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Quote:
LocN9ne said:
Quote:
Supalemonhaze said: Hey loc, do you have any experience with meshed in molds?
Not really that I'm aware of... Its usually "meshed" in bacteria that I have to deal with... The molds I get are usually just like that picture because I get sloppy as Fuck in the SAB... If I'm doing more than like 30 transfers I need a fucking cigarette so bad by the time I'm nearing the end that I just Fuck it all up...
So why not take a break half way through and smoke a cigarette? Let the air settle so any mistakes made before would have a smaller chance at affecting the second half of work.
I don't smoke, but if I notice that I am making too many large movements and fucking things up, I close up the box and wait 20 minutes. Plus it's just nice to have a break when you are working in a SAB for close to or over an hour.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: contamination pic of the day [Re: GreenRabbit]
#23152117 - 04/25/16 01:14 AM (7 years, 9 months ago) |
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Meh, I would want to get it over with. It's not uncommon for me to have a 2hr session and if I wanted to stop to smoke whenever I felt I needed one, I would turn that into a 3hr session. I use a hood so I wouldn't benefit from stopping. Tyvek sleeves are also a bitch to tuck under the gloves sometimes so the less time I spend fiddling, the better.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Sounds like you need to tighten up your movements. I don't even use tyvek, bare arms and gloves for me. I rarely see contamination due to inoculate. A spot on a plate outta 80-100 or so. My biggest issue to date was grain with high CFU populations. This is done in a SAB in a moldy basement.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: contamination pic of the day [Re: Pastywhyte]
#23152490 - 04/25/16 07:16 AM (7 years, 9 months ago) |
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I don't see much contamination on plates either nowadays. This week I've been panicking about possible meshed molds but when it comes to contam spots on plates, I'm comfortable with the number I get.
Now I'm not sure about this but I get the idea that when you have a meshed in mold, it is usually because you kept transferring it from a spore inoculated plate, right? And that for them to grow together they need to germinate around the same time and around the same spot.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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This oyster jar is not nearly as disgusting as most of the stuff here but I'm still kind of put off from cleaning it out.

LICK IT! yum.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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What do you guys think? Are those lone hypae from hidden molds? These are 2 day old transfers from suspect plates. If this is so this might turn out to be a reliable way to spot meshed molds. Might also be a good addition to the hidden contams thread.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Boooooo..
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Q&A US vs. THEM The more I learn, the less I know.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: contamination pic of the day [Re: LocN9ne]
#23172984 - 04/30/16 02:33 PM (7 years, 8 months ago) |
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