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OfflineBugler Boy
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Registered: 09/19/11
Posts: 998
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Last seen: 2 years, 8 months
G2G Noob.. Advice Needed
    #22220401 - 09/11/15 06:47 AM (8 years, 4 months ago)

Getting into G2G.

Heres my SAB

This is going to be my first run.. I have 21 jars prepped for the G2G and lots of spawn.

Main question is how many master jars should I use to inoc the fresh 21? They are just under 2/3 filled. Is 1 master jar --> 7 new jars too much or should i just use 2 masters and do 10 and 11?


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"


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InvisibleMunchauzen
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Re: G2G Noob.. Advice Needed [Re: Bugler Boy]
    #22220432 - 09/11/15 06:57 AM (8 years, 4 months ago)

I just did 24 jars with 3 jars of spawn.


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InvisibleLeopardMan
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Re: G2G Noob.. Advice Needed [Re: Bugler Boy]
    #22220772 - 09/11/15 08:42 AM (8 years, 4 months ago)

Well, there is no rule here. In theory, you just need one grain to colonize an entire jar, but of course you want to speed up the process and use more. You can't go wrong with a 1:3 or 1:5 ratio.


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-


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OfflinePussyFart
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Re: G2G Noob.. Advice Needed [Re: Bugler Boy]
    #22220885 - 09/11/15 09:05 AM (8 years, 4 months ago)

Quote:

Bugler Boy said:
Getting into G2G.

Heres my SAB




I like it.....but you might find the arm holes are a little to close together....I spread mine apart to match my shoulder width, it's now perfect.

Just a heads up.


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OfflineBugler Boy
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Registered: 09/19/11
Posts: 998
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Re: G2G Noob.. Advice Needed [Re: PussyFart]
    #22221501 - 09/11/15 11:35 AM (8 years, 4 months ago)

Quote:

Munchauzen said:
I just did 24 jars with 3 jars of spawn.



Quote:

LeopardMan said:
Well, there is no rule here. In theory, you just need one grain to colonize an entire jar, but of course you want to speed up the process and use more. You can't go wrong with a 1:3 or 1:5 ratio.




Cool thanks, I'll probably just end up doing the 21 with 3 jars since I have some to spare.

Quote:

PussyFart said:
I like it.....but you might find the arm holes are a little to close together....I spread mine apart to match my shoulder width, it's now perfect.

Just a heads up.




Thank you! I'll give it a go on this first run and see how it is but I see what you're talking about when I get sat in front of it... only be like $10 to grab a new tub :shrug:


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"


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Invisiblenatedawgnow
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Re: G2G Noob.. Advice Needed [Re: Bugler Boy]
    #22221557 - 09/11/15 11:41 AM (8 years, 4 months ago)

Just tape that side up real good and melt holes in
the other side. No need to waste a perfectly good tub on
a minor mistake.


--------------------


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InvisibleMunchauzen
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Re: G2G Noob.. Advice Needed [Re: natedawgnow]
    #22221562 - 09/11/15 11:41 AM (8 years, 4 months ago)

Quote:

natedawgnow said:
Just tape that side up real good and melt holes in
the other side. No need to waste a perfectly good tub on
a minor mistake.




would work!


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OfflineBugler Boy
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Registered: 09/19/11
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Re: G2G Noob.. Advice Needed [Re: Munchauzen]
    #22244432 - 09/16/15 05:35 AM (8 years, 4 months ago)

Here's 3 days after the G2G, how are they looking?





p.s @ PussyFart.. the holes were just a touch too close so for the $10 i think i'll be fixing my mistake. Thanks again for the advice :thumbup:


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"


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Offlinemegatacular
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Re: G2G Noob.. Advice Needed [Re: PussyFart]
    #22244499 - 09/16/15 06:18 AM (8 years, 4 months ago)

Quote:

PussyFart said:
Quote:

Bugler Boy said:
Getting into G2G.

Heres my SAB




I like it.....but you might find the arm holes are a little to close together....I spread mine apart to match my shoulder width, it's now perfect.

Just a heads up.






This mine. It looks like they're close but it is a big tote. They are a tad too far apart for me but I like em. I used soft ultra flex silicone for everything to the PVC actually gives quite a bit for extra reach. I cut and sealed the.bottom with 1/4" plexi and I use a 10:1 ratio of bleach and water to soak a towel to set it on then fold it up around the.edges. Aldo I cut off the gloves to stop the compression and vacuum caused by pulling hands out.and.putting.back in. They're basicly flaps to kinda keep airnfrom moving.too much when I do reach in. I wear long dish washing gloves and tyvek sleeves as well. Spray with a little Lysol let sit for an hr.or so and I've have 0 contams to anything so far. Except from a syringe to agar once. But that was in the.ms syringe. Maybe it helps. Sorry if useless info. I've never.done g2g but will soon. Good luck mayne


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Invisiblefilthyknees
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Re: G2G Noob.. Advice Needed [Re: Bugler Boy]
    #22244503 - 09/16/15 06:19 AM (8 years, 4 months ago)

looks like you distributed to only one side of the jar, this will mean slower colonization.
next time rotate the jar a good 1 or 2 times while shaking top to bottom to get an even spread for faster colonization. :super:

would say that's ready for shakin'


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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Offlinemegatacular
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Re: G2G Noob.. Advice Needed [Re: megatacular]
    #22244511 - 09/16/15 06:27 AM (8 years, 4 months ago)



Another over top. Sorry the lights are in the way. It is super clear though. Just wanted to show the towel and how my gloves are cut short. About to do some transfers of this bad boy.





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Invisiblefilthyknees
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Re: G2G Noob.. Advice Needed [Re: megatacular]
    #22244520 - 09/16/15 06:33 AM (8 years, 4 months ago)

hope you're wetting that towel before using your box. soap & water spray?

looks like a mofo of a culture dude :cool:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineBugler Boy
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Registered: 09/19/11
Posts: 998
Loc: In your head
Last seen: 2 years, 8 months
Re: G2G Noob.. Advice Needed [Re: megatacular]
    #22244529 - 09/16/15 06:39 AM (8 years, 4 months ago)

Quote:

filthyknees said:
looks like you distributed to only one side of the jar, this will mean slower colonization.
next time rotate the jar a good 1 or 2 times while shaking top to bottom to get an even spread for faster colonization. :super:

would say that's ready for shakin'




Everything I've read from TC's says shake once after G2G then wait for 100%. That myc doesn't even look fully recovered I don't think I'm going to shake again or at least a solid few days

Quote:

megatacular said:
Good luck mayne




You too. Nice innovation on a colored tote


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"


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Offlinemegatacular
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Re: G2G Noob.. Advice Needed [Re: filthyknees]
    #22244531 - 09/16/15 06:42 AM (8 years, 4 months ago)

On yeah I explained it in the post above. I wash then whole run in my jacuzzi soap and water. Then the towel is soaked real good with 10:1 water and bleach to kell anything that settles on it. Its folded up around the bottom edges to catch anything in the air if it does move and suck air in.through the bottom. I don't.even have to try hard at all to be as clean as.possible in it. I've made 46 agar plates two lc jars and 22 transfers and 12 grain jar knock ups open lids agar wedges not string. And only one plate had a tinyspec of bacteria. That's it.


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Offlinemegatacular
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Re: G2G Noob.. Advice Needed [Re: megatacular]
    #22244539 - 09/16/15 06:45 AM (8 years, 4 months ago)

Quote:

Bugler Boy said:
Quote:

filthyknees said:
looks like you distributed to only one side of the jar, this will mean slower colonization.
next time rotate the jar a good 1 or 2 times while shaking top to bottom to get an even spread for faster colonization. :super:

would say that's ready for shakin'




Everything I've read from TC's says shake once after G2G then wait for 100%. That myc doesn't even look fully recovered I don't think I'm going to shake again or at least a solid few days

Quote:

megatacular said:
Good luck mayne




You too. Nice innovation on a colored tote




Just playing my part here on the shroomery


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Invisiblefilthyknees
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Re: G2G Noob.. Advice Needed [Re: Bugler Boy]
    #22244577 - 09/16/15 07:01 AM (8 years, 4 months ago)

Quote:

Bugler Boy said:
Everything I've read from TC's says shake once after G2G then wait for 100%. That myc doesn't even look fully recovered I don't think I'm going to shake again or at least a solid few days





once when you make the transfer, again at ~30% :super:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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Offlinemidnightmaraude
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Re: G2G Noob.. Advice Needed [Re: megatacular]
    #22244639 - 09/16/15 07:20 AM (8 years, 4 months ago)

Quote:

megatacular said:
Quote:

Bugler Boy said:
Quote:

filthyknees said:
looks like you distributed to only one side of the jar, this will mean slower colonization.
next time rotate the jar a good 1 or 2 times while shaking top to bottom to get an even spread for faster colonization. :super:

would say that's ready for shakin'




Everything I've read from TC's says shake once after G2G then wait for 100%. That myc doesn't even look fully recovered I don't think I'm going to shake again or at least a solid few days

Quote:

megatacular said:
Good luck mayne




You too. Nice innovation on a colored tote




Just playing my part here on the shroomery




:notimpressed:


Very good man.  My box has a small glass window (where'd you get plexi
?)

I also don't the pvc inserts where the holes are and I figure I definitely need to add them now along with the dish gloves cut off and glued to the end of it.  WOW man...


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Offlinemegatacular
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Re: G2G Noob.. Advice Needed [Re: midnightmaraude]
    #22244679 - 09/16/15 07:35 AM (8 years, 4 months ago)

Plexi at home depot. I duct tapes my gloves on then just cut off the.hands. glued the 4 inch couplings on both sides. Pretty expensive.but I love.it. works flawlessly.
Spray a little Lysol in and let settle for an hr and.dive.in.


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Offlinemidnightmaraude
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Re: G2G Noob.. Advice Needed [Re: megatacular]
    #22244716 - 09/16/15 07:50 AM (8 years, 4 months ago)

Quote:

megatacular said:
Plexi at home depot. I duct tapes my gloves on then just cut off the.hands. glued the 4 inch couplings on both sides. Pretty expensive.but I love.it. works flawlessly.
Spray a little Lysol in and let settle for an hr and.dive.in.




I'll be doing this.  How did you make the holes?  I used a coffee can.  Will the 4" pvc fit in those?


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OfflinePussyFart
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Re: G2G Noob.. Advice Needed [Re: filthyknees]
    #22244718 - 09/16/15 07:52 AM (8 years, 4 months ago)

Quote:

filthyknees said:
Quote:

Bugler Boy said:
Everything I've read from TC's says shake once after G2G then wait for 100%. That myc doesn't even look fully recovered I don't think I'm going to shake again or at least a solid few days





once when you make the transfer, again at ~30% :super:



No.....g2g jars should only need to be shaken once, right after the transfer.....not after 30%.....


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Invisiblefilthyknees
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Re: G2G Noob.. Advice Needed [Re: PussyFart]
    #22244743 - 09/16/15 08:03 AM (8 years, 4 months ago)

been shaking at 30% for years :blazed:

to clarify you never break up your grain jars?? only when they're about to go into the tubs?? I have always shaken at 30%.  :shrug2:

wow.. mind blown. :shrug: I'll try with a few...


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


Extras: Filter Print Post Top
OfflinePussyFart
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Re: G2G Noob.. Advice Needed [Re: filthyknees]
    #22244749 - 09/16/15 08:04 AM (8 years, 4 months ago)

Well if you shake right after a g2g transfer, you just spread out the inoculation points, which is the exact reason you normally shake at 30% or so.....

If the points are already spread out, there is no need to shake...it's definielty not going to save u any time...it's just adding time for recovery.


Edited by PussyFart (09/16/15 08:05 AM)


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OfflineBugler Boy
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Registered: 09/19/11
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Re: G2G Noob.. Advice Needed [Re: PussyFart]
    #22244815 - 09/16/15 08:31 AM (8 years, 4 months ago)

Quote:

PussyFart said:
Well if you shake right after a g2g transfer, you just spread out the inoculation points, which is the exact reason you normally shake at 30% or so.....

If the points are already spread out, there is no need to shake...it's definielty not going to save u any time...it's just adding time for recovery.




Thank you :thumbup:

Clarifying my searches in real time lol


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"


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Offlinemegatacular
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Re: G2G Noob.. Advice Needed [Re: midnightmaraude]
    #22244868 - 09/16/15 08:45 AM (8 years, 4 months ago)

Quote:

midnightmaraude said:
Quote:

megatacular said:
Plexi at home depot. I duct tapes my gloves on then just cut off the.hands. glued the 4 inch couplings on both sides. Pretty expensive.but I love.it. works flawlessly.
Spray a little Lysol in and let settle for an hr and.dive.in.




I'll be doing this.  How did you make the holes?  I used a coffee can.  Will the 4" pvc fit in those?




Not.sure about the.coffee can. I measured the couplings set my jig on my router drilled small hole for the pivot.pin and beeeerrrrrrrnnnnnt it was done. Lol


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Offlinemegatacular
professor procrastinate
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Re: G2G Noob.. Advice Needed [Re: Bugler Boy]
    #22244880 - 09/16/15 08:50 AM (8 years, 4 months ago)

Quote:

Bugler Boy said:
Quote:

PussyFart said:
Well if you shake right after a g2g transfer, you just spread out the inoculation points, which is the exact reason you normally shake at 30% or so.....

If the points are already spread out, there is no need to shake...it's definielty not going to save u any time...it's just adding time for recovery.




Thank you :thumbup:

Clarifying my searches in real time lol




10-4 same here. Good to know. What about if ur knocking.up with agar and or lc? Is that when u shake at 30%? I ask cause I'll be doing both as soon as my lc is good to go and my mono cultures I cloned from this one


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OfflineBugler Boy
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Registered: 09/19/11
Posts: 998
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Last seen: 2 years, 8 months
Re: G2G Noob.. Advice Needed [Re: megatacular]
    #22245147 - 09/16/15 10:04 AM (8 years, 4 months ago)

Quote:

megatacular said:

10-4 same here. Good to know. What about if ur knocking.up with agar and or lc? Is that when u shake at 30%? I ask cause I'll be doing both as soon as my lc is good to go and my mono cultures I cloned from this one





These G2G were from MS and I shook once at this point.

~30% goes for agar and LC too, yes


--------------------
The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"


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