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OfflinefirstTIMER420
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Pan Cyan germination
    #22219015 - 09/10/15 09:50 PM (8 years, 4 months ago)

Been trying for the past three weeks to start pan cyan spores on agar, but seem to be hitting some problems.  Ive done three rounds of agar, used a MS syringe to innoc them and for the last round used prints to inoculate.  For some reason, the spores aren't germinating  on the agar from either the syringe or print.

The strange part, is that I made two jars last round I did some grains and inoculated them with the same syringe, and they germinated and are almost fully colonized.  Im confused, I figured maybe the spores were really old and dried out, or something.

If you guys have any advice on what to do, it would be awesome.  Im really looking forward to growing some pans.


--Also, ive been looking at pics of the pan cyan mycelium, and its kind of hard to tell exactly what it looks like.  If you have some pics of it thatd be cool as well.


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Re: Pan Cyan germination [Re: firstTIMER420]
    #22219165 - 09/10/15 10:29 PM (8 years, 4 months ago)

If I may ask, what attracts you to pan cyans?


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OfflineCTDreamer203
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Re: Pan Cyan germination [Re: champinhom]
    #22219173 - 09/10/15 10:33 PM (8 years, 4 months ago)

Higher potency is usually what attracts most people


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OfflineICEMANOO9
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Re: Pan Cyan germination [Re: CTDreamer203]
    #22219207 - 09/10/15 10:42 PM (8 years, 4 months ago)

Hey These were germ on pda hpoo agar than tiger drop to WBS grain hpoo mix. It's super aggressive  this is pan cyan Hawaii  look up pan cyan on YouTube and watch the video by Dave Barlow



Edited by ICEMANOO9 (09/10/15 10:49 PM)


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219238 - 09/10/15 10:48 PM (8 years, 4 months ago)

You have to mix a litte horse poo in your agar apparently. Because I did that and surprise it germinated

I transferred once away from this dish that had some contams

Good luck let me know any q's you might wanna ask


Edited by ICEMANOO9 (09/10/15 11:07 PM)


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OfflinefirstTIMER420
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Re: Pan Cyan germination [Re: champinhom]
    #22219402 - 09/10/15 11:31 PM (8 years, 4 months ago)

yup, higher potency and the fact that ive grown cubes and tripped on them a lot.

the weird thing is that the MS syringe I used on the agar germinated on 2 jars of grains.

so, if the spores were old or dried out, they wouldn't have done that.


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Re: Pan Cyan germination [Re: firstTIMER420]
    #22219431 - 09/10/15 11:39 PM (8 years, 4 months ago)

Maybe use a different base for your agar.. A little h poo like iceman said might just do the trick! :solidnod:


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Re: Pan Cyan germination [Re: CTDreamer203]
    #22219445 - 09/10/15 11:41 PM (8 years, 4 months ago)

I can guarantee it isn't the poo. I use a 2% malt extract and 2% agar recipe. Never had problems with pan myc on agar


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219460 - 09/10/15 11:45 PM (8 years, 4 months ago)

Quote:

ICEMANOO9 said:
You have to mix a litte horse poo in your agar apparently. Because I did that and surprise it germinated

I transferred once away from this dish that had some contams

Good luck let me know any q's you might wanna ask



That whole plate looks like contams


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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219465 - 09/10/15 11:46 PM (8 years, 4 months ago)

:whathesaid: :lol:


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Re: Pan Cyan germination [Re: Mad Season]
    #22219480 - 09/10/15 11:53 PM (8 years, 4 months ago)

lol yea looks like it but I did manage to get a clean xfer come on that gray shit doesn't look like pan myc to you??


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Re: Pan Cyan germination [Re: Mad Season]
    #22219488 - 09/10/15 11:55 PM (8 years, 4 months ago)

Just looks like moldy horse shit :lol: dont think that's the mycelium we are searching for I didn't even notice his pic... I immediately withdrawal my statement about the h poo :facepalm: :facepalm3:


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219498 - 09/10/15 11:57 PM (8 years, 4 months ago)



Extremely clean pan myc


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Re: Pan Cyan germination [Re: Mad Season]
    #22219504 - 09/10/15 11:58 PM (8 years, 4 months ago)

:rockon:


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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219529 - 09/11/15 12:09 AM (8 years, 4 months ago)

???


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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219530 - 09/11/15 12:10 AM (8 years, 4 months ago)

Shit happens, your putting more then one drop in a jar


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Re: Pan Cyan germination [Re: cronicr]
    #22219546 - 09/11/15 12:18 AM (8 years, 4 months ago)

What else would have the tenacity of colonizing this jar after I tiger dropped? If not some sort of mycelium? It surely can't be some fucking jar of cobweb mold?



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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219555 - 09/11/15 12:22 AM (8 years, 4 months ago)

Those don't look very...good


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219558 - 09/11/15 12:23 AM (8 years, 4 months ago)

Pan cyan mycelium is black?


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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219561 - 09/11/15 12:24 AM (8 years, 4 months ago)

Quote:

PinPornProducer said:
Those don't look very...good



Be honest they look fucked


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Re: Pan Cyan germination [Re: cronicr]
    #22219564 - 09/11/15 12:26 AM (8 years, 4 months ago)

:whathesaid:


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Re: Pan Cyan germination [Re: cronicr]
    #22219569 - 09/11/15 12:26 AM (8 years, 4 months ago)

I was being nice lol


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Re: Pan Cyan germination [Re: Mad Season]
    #22219570 - 09/11/15 12:26 AM (8 years, 4 months ago)

one of the plates I have looks like that, but I think I mislabeled as I was also doing cubes at the same time.

Im going to post a couple pics of the few plates so far that have colonized.


But you know how cube mycelium doesn't really come off the plate, from what im seeing pan myc does? cottony looking?  Also, whats the common time span for germination of these plates.


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Re: Pan Cyan germination [Re: Mad Season]
    #22219571 - 09/11/15 12:27 AM (8 years, 4 months ago)

Don't ya hate it when ya post that and it ends up on the next page


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Re: Pan Cyan germination [Re: cronicr]
    #22219592 - 09/11/15 12:33 AM (8 years, 4 months ago)

:lol: tbh i have it so that I get 99 posts per page. But I do hate it when it happens on the 100th post :lol:

@first timer, I made a pan cyan plate from spores on the 1st. It germinated by the 6th (5 days)


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Edited by Mad Season (09/11/15 12:35 AM)


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OfflineICEMANOO9
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Re: Pan Cyan germination [Re: Mad Season]
    #22219622 - 09/11/15 12:41 AM (8 years, 4 months ago)

First timer yes this black mycelium that too it "jumped " off the agar cottony in all 360degrees. i know exactly what your describing I thougt it was cob web mold..


Than what the heck is this?? I know it's not isolated all pro like as you all achieve but it has to be pan  myc it's just gots to



Edited by ICEMANOO9 (09/11/15 12:50 AM)


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219665 - 09/11/15 12:56 AM (8 years, 4 months ago)

:wellthatshorrifying: is what it is. Pan myc is supposed to be extremely cottony, white, definitely not black/brown :lol:.


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Edited by Mad Season (09/11/15 12:57 AM)


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OfflineICEMANOO9
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Re: Pan Cyan germination [Re: Mad Season]
    #22219672 - 09/11/15 12:59 AM (8 years, 4 months ago)

:learning: :whyyy:  :freud:  :youdidit:  :underage:


Edited by ICEMANOO9 (09/11/15 01:03 AM)


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219699 - 09/11/15 01:13 AM (8 years, 4 months ago)

Quote:

ICEMANOO9 said:
First timer yes this black mycelium that too it "jumped " off the agar cottony in all 360degrees. i know exactly what your describing I thougt it was cob web mold..


Than what the heck is this?? I know it's not isolated all pro like as you all achieve but it has to be pan  myc it's just gots to





Why haven't you tossed those out yet lol?


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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219717 - 09/11/15 01:18 AM (8 years, 4 months ago)

Wtf is it than??


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OfflinefirstTIMER420
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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219735 - 09/11/15 01:22 AM (8 years, 4 months ago)

Quote:

PinPornProducer said:
Quote:

ICEMANOO9 said:
First timer yes this black mycelium that too it "jumped " off the agar cottony in all 360degrees. i know exactly what your describing I thougt it was cob web mold..


Than what the heck is this?? I know it's not isolated all pro like as you all achieve but it has to be pan  myc it's just gots to





Why haven't you tossed those out yet lol?





Jesus Christ.  dude, I have tried the PF style cakes for pans, they didn't do shit for me. just saying. no contams, but no colonization.


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219757 - 09/11/15 01:27 AM (8 years, 4 months ago)

Pm kizzle? Lol.. so many molds in that colour range. You can also put the black stuff under a scope and it'd be even easier to identify


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Re: Pan Cyan germination [Re: firstTIMER420]
    #22219760 - 09/11/15 01:28 AM (8 years, 4 months ago)

Fuck that ima case it


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Re: Pan Cyan germination [Re: ICEMANOO9] * 1
    #22219762 - 09/11/15 01:28 AM (8 years, 4 months ago)



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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219765 - 09/11/15 01:29 AM (8 years, 4 months ago)

Quote:

ICEMANOO9 said:
Fuck that ima case it



:goodluckwiththat2:


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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219773 - 09/11/15 01:32 AM (8 years, 4 months ago)

:sped:


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219774 - 09/11/15 01:32 AM (8 years, 4 months ago)

If you really want to ruin your future projects by spreading that black shit, and the trich, cobweb and whatever clusterfuck of contams will form if you remove that and attempt to do anything with it, then go for it.


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Re: Pan Cyan germination [Re: firstTIMER420]
    #22219793 - 09/11/15 01:38 AM (8 years, 4 months ago)

I'll take your advice and absolutely not give a shit.


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219797 - 09/11/15 01:40 AM (8 years, 4 months ago)

How about you make your own thread and not ruin this one


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Re: Pan Cyan germination [Re: cronicr]
    #22219809 - 09/11/15 01:44 AM (8 years, 4 months ago)

my bad firstimer bummed these are just mold or whatever


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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219821 - 09/11/15 01:49 AM (8 years, 4 months ago)

just take it easy lol
idk what it is to be exact but it ain't good


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Re: Pan Cyan germination [Re: cronicr]
    #22219835 - 09/11/15 01:55 AM (8 years, 4 months ago)

Ok. I'ma whip up some pasty plates I'm interested in seeing your pics firsttimer


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Re: Pan Cyan germination [Re: cronicr]
    #22219884 - 09/11/15 02:28 AM (8 years, 4 months ago)

thank you cron.

like I said, here are the pics.  I was starting some pink buffalo that day and I think I may have mislabeled these plates, I mixed them up before I labeled them.

as for the other plate, its just starting out, but there is this fuzzy white growth going up the side of the wall, stopped about 3/4 way up, and is now just growing outwards.

the pink buffalo(what ill call it for now) plate that has two sized growing on it, I remember placing two drops of solution on it, one on each side. but what im confused about is that when it started growing, there was an even line between the two sides of growth dividing them, this is what made me think it was a contam instead of myc.  Along with that, and the fact that whatever it is seems to be growing upwards, which I have never seen cube myc do.
The only one I know for sure that was innoc'd with pan is the 4th pic



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Edited by firstTIMER420 (09/11/15 02:30 AM)


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Re: Pan Cyan germination [Re: firstTIMER420]
    #22220817 - 09/11/15 08:52 AM (8 years, 4 months ago)

Let the pans grow out more, too early to tell imo, and that pb looks more like tomentose cube myc :shrug:


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Re: Pan Cyan germination [Re: Mad Season]
    #22220895 - 09/11/15 09:07 AM (8 years, 4 months ago)

gotcha.  I had noticed from the growth of the jars using the PB MS that the myc seemed more cottony than rhizo.  I guess that's why the culture turned out that way.  But, that being said. Ive never seen myc divide itself symmetrically and keep itself separated like that, which is what made me think it contamed.  Im nervous about opening it up because I don't want to spread mold around if it isn't myc


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Re: Pan Cyan germination [Re: firstTIMER420]
    #22221221 - 09/11/15 10:25 AM (8 years, 4 months ago)

Sporulate molds aren't white. Also you should open it up in a still air box. Never had a problem with moldy plates in it.


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OfflinefirstTIMER420
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Re: Pan Cyan germination [Re: Mad Season]
    #22229449 - 09/12/15 11:09 PM (8 years, 4 months ago)

well, turn of events.  The plate I thougjt was pan cyan started turning brown.  guess you were right about molds lol.

also, like I said, I have another plate growing I think and started a few more today. Using a new technique this time to start from a print.

Here are my pan cyan jars, shook em a week ago I think, do they need more time or are they about ready?



(one of the jars is PE, and after I shook it, a couple spots are turning blue, is that normal?)


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