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firstTIMER420
Born the son of a sharecropper..


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Pan Cyan germination
#22219015 - 09/10/15 09:50 PM (8 years, 4 months ago) |
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Been trying for the past three weeks to start pan cyan spores on agar, but seem to be hitting some problems. Ive done three rounds of agar, used a MS syringe to innoc them and for the last round used prints to inoculate. For some reason, the spores aren't germinating on the agar from either the syringe or print.
The strange part, is that I made two jars last round I did some grains and inoculated them with the same syringe, and they germinated and are almost fully colonized. Im confused, I figured maybe the spores were really old and dried out, or something.
If you guys have any advice on what to do, it would be awesome. Im really looking forward to growing some pans.
--Also, ive been looking at pics of the pan cyan mycelium, and its kind of hard to tell exactly what it looks like. If you have some pics of it thatd be cool as well.
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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If I may ask, what attracts you to pan cyans?
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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CTDreamer203
Fun Guy



Registered: 08/12/15
Posts: 247
Loc: United States
Last seen: 3 years, 10 months
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Re: Pan Cyan germination [Re: champinhom]
#22219173 - 09/10/15 10:33 PM (8 years, 4 months ago) |
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Higher potency is usually what attracts most people
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"The Universe is an infinite exchange of knowledge"-Vincent John Brazeau III
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ICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
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Hey These were germ on pda hpoo agar than tiger drop to WBS grain hpoo mix. It's super aggressive this is pan cyan Hawaii look up pan cyan on YouTube and watch the video by Dave Barlow
Edited by ICEMANOO9 (09/10/15 10:49 PM)
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ICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
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Re: Pan Cyan germination [Re: ICEMANOO9]
#22219238 - 09/10/15 10:48 PM (8 years, 4 months ago) |
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You have to mix a litte horse poo in your agar apparently. Because I did that and surprise it germinated
 I transferred once away from this dish that had some contams
Good luck let me know any q's you might wanna ask
Edited by ICEMANOO9 (09/10/15 11:07 PM)
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firstTIMER420
Born the son of a sharecropper..


Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Pan Cyan germination [Re: champinhom]
#22219402 - 09/10/15 11:31 PM (8 years, 4 months ago) |
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yup, higher potency and the fact that ive grown cubes and tripped on them a lot.
the weird thing is that the MS syringe I used on the agar germinated on 2 jars of grains.
so, if the spores were old or dried out, they wouldn't have done that.
-------------------- 1 2 3 4 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url] --Anything posted by this account is completely fictitious--
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CTDreamer203
Fun Guy



Registered: 08/12/15
Posts: 247
Loc: United States
Last seen: 3 years, 10 months
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Maybe use a different base for your agar.. A little h poo like iceman said might just do the trick!
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"The Universe is an infinite exchange of knowledge"-Vincent John Brazeau III
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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I can guarantee it isn't the poo. I use a 2% malt extract and 2% agar recipe. Never had problems with pan myc on agar
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Pan Cyan germination [Re: ICEMANOO9]
#22219460 - 09/10/15 11:45 PM (8 years, 4 months ago) |
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Quote:
ICEMANOO9 said: You have to mix a litte horse poo in your agar apparently. Because I did that and surprise it germinated
 I transferred once away from this dish that had some contams
Good luck let me know any q's you might wanna ask
That whole plate looks like contams
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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ICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
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Re: Pan Cyan germination [Re: Mad Season]
#22219480 - 09/10/15 11:53 PM (8 years, 4 months ago) |
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lol yea looks like it but I did manage to get a clean xfer come on that gray shit doesn't look like pan myc to you??
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CTDreamer203
Fun Guy



Registered: 08/12/15
Posts: 247
Loc: United States
Last seen: 3 years, 10 months
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Re: Pan Cyan germination [Re: Mad Season]
#22219488 - 09/10/15 11:55 PM (8 years, 4 months ago) |
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Just looks like moldy horse shit dont think that's the mycelium we are searching for I didn't even notice his pic... I immediately withdrawal my statement about the h poo
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"The Universe is an infinite exchange of knowledge"-Vincent John Brazeau III
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Pan Cyan germination [Re: ICEMANOO9]
#22219498 - 09/10/15 11:57 PM (8 years, 4 months ago) |
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Extremely clean pan myc
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Pan Cyan germination [Re: Mad Season]
#22219504 - 09/10/15 11:58 PM (8 years, 4 months ago) |
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ICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
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???
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Shit happens, your putting more then one drop in a jar
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
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Re: Pan Cyan germination [Re: cronicr]
#22219546 - 09/11/15 12:18 AM (8 years, 4 months ago) |
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What else would have the tenacity of colonizing this jar after I tiger dropped? If not some sort of mycelium? It surely can't be some fucking jar of cobweb mold?
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: Pan Cyan germination [Re: ICEMANOO9]
#22219555 - 09/11/15 12:22 AM (8 years, 4 months ago) |
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Those don't look very...good
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CTDreamer203
Fun Guy



Registered: 08/12/15
Posts: 247
Loc: United States
Last seen: 3 years, 10 months
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Re: Pan Cyan germination [Re: ICEMANOO9]
#22219558 - 09/11/15 12:23 AM (8 years, 4 months ago) |
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Pan cyan mycelium is black?
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"The Universe is an infinite exchange of knowledge"-Vincent John Brazeau III
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
PinPornProducer said: Those don't look very...good
Be honest they look fucked
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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