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OfflinefirstTIMER420
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Pan Cyan germination
    #22219015 - 09/10/15 09:50 PM (8 years, 4 months ago)

Been trying for the past three weeks to start pan cyan spores on agar, but seem to be hitting some problems.  Ive done three rounds of agar, used a MS syringe to innoc them and for the last round used prints to inoculate.  For some reason, the spores aren't germinating  on the agar from either the syringe or print.

The strange part, is that I made two jars last round I did some grains and inoculated them with the same syringe, and they germinated and are almost fully colonized.  Im confused, I figured maybe the spores were really old and dried out, or something.

If you guys have any advice on what to do, it would be awesome.  Im really looking forward to growing some pans.


--Also, ive been looking at pics of the pan cyan mycelium, and its kind of hard to tell exactly what it looks like.  If you have some pics of it thatd be cool as well.


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Invisiblechampinhom
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Re: Pan Cyan germination [Re: firstTIMER420]
    #22219165 - 09/10/15 10:29 PM (8 years, 4 months ago)

If I may ask, what attracts you to pan cyans?


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OfflineCTDreamer203
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Re: Pan Cyan germination [Re: champinhom]
    #22219173 - 09/10/15 10:33 PM (8 years, 4 months ago)

Higher potency is usually what attracts most people


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OfflineICEMANOO9
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Re: Pan Cyan germination [Re: CTDreamer203]
    #22219207 - 09/10/15 10:42 PM (8 years, 4 months ago)

Hey These were germ on pda hpoo agar than tiger drop to WBS grain hpoo mix. It's super aggressive  this is pan cyan Hawaii  look up pan cyan on YouTube and watch the video by Dave Barlow



Edited by ICEMANOO9 (09/10/15 10:49 PM)


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OfflineICEMANOO9
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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219238 - 09/10/15 10:48 PM (8 years, 4 months ago)

You have to mix a litte horse poo in your agar apparently. Because I did that and surprise it germinated

I transferred once away from this dish that had some contams

Good luck let me know any q's you might wanna ask


Edited by ICEMANOO9 (09/10/15 11:07 PM)


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OfflinefirstTIMER420
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Re: Pan Cyan germination [Re: champinhom]
    #22219402 - 09/10/15 11:31 PM (8 years, 4 months ago)

yup, higher potency and the fact that ive grown cubes and tripped on them a lot.

the weird thing is that the MS syringe I used on the agar germinated on 2 jars of grains.

so, if the spores were old or dried out, they wouldn't have done that.


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OfflineCTDreamer203
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Re: Pan Cyan germination [Re: firstTIMER420]
    #22219431 - 09/10/15 11:39 PM (8 years, 4 months ago)

Maybe use a different base for your agar.. A little h poo like iceman said might just do the trick! :solidnod:


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InvisibleMad Season
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Re: Pan Cyan germination [Re: CTDreamer203]
    #22219445 - 09/10/15 11:41 PM (8 years, 4 months ago)

I can guarantee it isn't the poo. I use a 2% malt extract and 2% agar recipe. Never had problems with pan myc on agar


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OfflinePinPornProducer
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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219460 - 09/10/15 11:45 PM (8 years, 4 months ago)

Quote:

ICEMANOO9 said:
You have to mix a litte horse poo in your agar apparently. Because I did that and surprise it germinated

I transferred once away from this dish that had some contams

Good luck let me know any q's you might wanna ask



That whole plate looks like contams


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InvisibleMad Season
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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219465 - 09/10/15 11:46 PM (8 years, 4 months ago)

:whathesaid: :lol:


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineICEMANOO9
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Re: Pan Cyan germination [Re: Mad Season]
    #22219480 - 09/10/15 11:53 PM (8 years, 4 months ago)

lol yea looks like it but I did manage to get a clean xfer come on that gray shit doesn't look like pan myc to you??


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OfflineCTDreamer203
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Re: Pan Cyan germination [Re: Mad Season]
    #22219488 - 09/10/15 11:55 PM (8 years, 4 months ago)

Just looks like moldy horse shit :lol: dont think that's the mycelium we are searching for I didn't even notice his pic... I immediately withdrawal my statement about the h poo :facepalm: :facepalm3:


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InvisibleMad Season
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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219498 - 09/10/15 11:57 PM (8 years, 4 months ago)



Extremely clean pan myc


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinePinPornProducer
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Re: Pan Cyan germination [Re: Mad Season]
    #22219504 - 09/10/15 11:58 PM (8 years, 4 months ago)

:rockon:


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OfflineICEMANOO9
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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219529 - 09/11/15 12:09 AM (8 years, 4 months ago)

???


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OfflinecronicrFacebook
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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219530 - 09/11/15 12:10 AM (8 years, 4 months ago)

Shit happens, your putting more then one drop in a jar


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OfflineICEMANOO9
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Re: Pan Cyan germination [Re: cronicr]
    #22219546 - 09/11/15 12:18 AM (8 years, 4 months ago)

What else would have the tenacity of colonizing this jar after I tiger dropped? If not some sort of mycelium? It surely can't be some fucking jar of cobweb mold?



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OfflinePinPornProducer
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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219555 - 09/11/15 12:22 AM (8 years, 4 months ago)

Those don't look very...good


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OfflineCTDreamer203
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Re: Pan Cyan germination [Re: ICEMANOO9]
    #22219558 - 09/11/15 12:23 AM (8 years, 4 months ago)

Pan cyan mycelium is black?


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OfflinecronicrFacebook
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Re: Pan Cyan germination [Re: PinPornProducer]
    #22219561 - 09/11/15 12:24 AM (8 years, 4 months ago)

Quote:

PinPornProducer said:
Those don't look very...good



Be honest they look fucked


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