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InvisibleWall.E
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419] * 1
    #27073156 - 12/05/20 08:54 AM (3 years, 1 month ago)

You could drive a prius on those things jake


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Offlinegt40
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Re: Official Copelandia/Panaeolus Thread [Re: Stipe-n Cap]
    #27073162 - 12/05/20 08:59 AM (3 years, 1 month ago)

Quote:

p9hu7 said:
Pretty sure they have grains in their sub.



angam didn't. he did spore syringe to manure only as I remember.
It works for him, but things just go super random each time with that approach I guess. Also I believe gentamicin sulfate was involved ) It's super common for his area.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: gt40]
    #27073169 - 12/05/20 09:00 AM (3 years, 1 month ago)

Are you sure he didn’t supplement grain in the bulk like BH does? I ran a tray without any grain LC noc’ed it Colonized fine but didn’t produce any fruits. I haven’t done enough controls to definitively say that they will definitely not grow without any grain supplementation but so far in my experience that is the case


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OfflineThe Dalcassian
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27073173 - 12/05/20 09:03 AM (3 years, 1 month ago)

Ya, it's in the first link in his Sig. A couple of comments down he says specifically he uses no grains he just nocc'd up a straw/manure mix but I'm pretty sure he does do agar first.


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Offlinegt40
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27073179 - 12/05/20 09:08 AM (3 years, 1 month ago)

Quote:

Jakeoncid419 said:
Are you sure he didn’t supplement grain in the bulk like BH does? I ran a tray without any grain LC noc’ed it Colonized fine but didn’t produce any fruits. I haven’t done enough controls to definitively say that they will definitely not grow without any grain supplementation but so far in my experience that is the case



I re-check his report, he didn't use grain at all. It's also common for growers of his area. But I don't really saw stable good results with that. I don't think it's good idea to exclude grain from mixture.
Personaly I had very strange colonization when tried LC -> straight manure few months ago.


Edited by gt40 (12/05/20 09:09 AM)


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: The Dalcassian]
    #27073182 - 12/05/20 09:10 AM (3 years, 1 month ago)

Yeah so he says no grain. He used a piece of colonised hay:manure to inoculate some of his jars, and syringes for others. But I'm not sure what's in the syringes, LI/LC/MS?


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: gt40]
    #27073183 - 12/05/20 09:11 AM (3 years, 1 month ago)

That’s interesting yeah I mean I only did one run without so it is definitely not solid proof that it will not work Blue helix and I have actually been discussing this back-and-forth trying to figure out grains effect on yield. I know for a fact if you go over 1 to 4 ratio you start running into problems. Cutting out grain completely would be awesome save me several hours of work and material cost so would only having to use LC and I am trying but as of right now grain to bulk is by far the most consistent optimal performer, for me at least


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Re: Official Copelandia/Panaeolus Thread [Re: The Dalcassian]
    #27073187 - 12/05/20 09:15 AM (3 years, 1 month ago)

Quote:

The Dalcassian said:
Ya, it's in the first link in his Sig. A couple of comments down he says specifically he uses no grains he just nocc'd up a straw/manure mix but I'm pretty sure he does do agar first.



I'm not his personal biographer )) but I know a bit about growing culture of russian speaking community. It's really really rare for them to use agar. Vice versa very common to use a ton of antibiotics - add it to spore syringes and soak grains with it also


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: gt40]
    #27073189 - 12/05/20 09:16 AM (3 years, 1 month ago)

nice pics jake🤩🤩🤩

i use a 1.4 grain to sub ratio.

when i make my substrate i pasteurize it all in jars on the stove but il make up a bunch over a weekend here n there at times. and when its cooled for 24hrs i bag it up into 4lb bags and freeze it in my big deep freezer til needed. that way when i do need it i just pull a bag out for each tray im doing  2 days prior to spawning to unthaw.  then i just open it up and dump in 1lb of colonized rye berries. and let it colonize in the bag. then when its at 100% il break it up and lay to my trays to recover.  once its recovered fully and had a day or two to consolidate a bit il case it and put it to fruit.


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27073190 - 12/05/20 09:16 AM (3 years, 1 month ago)

I'm mucking around with LC -> straw:manure at the moment. But I'm using pellets which I'm not super confident working with. I'm getting some proper manure on monday to test out. So far I've got a lot of myc but no fruit so far. But I haven't got any fruit from my rye -> straw:manure either.

I've also got two little pots with pan cambo agar wedge -> straw:manure.

On a side note. I've setup a tub FC similar to Angam's. With an aquarium heater and PC fans. However, I was wondering about the condenstatino on the lid dripping down on the tray. This could cause pins to abort right?


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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #27073192 - 12/05/20 09:22 AM (3 years, 1 month ago)

ive gone high as a 1.2 ratio jake with my jambos without any ill effect. thats actually where my starting point was and i started working my way down and noticed i get the same results at 1.4 as i did with 1.2 i mean zero difference. but i did notice a fairly big difference when i tried a 1.5 ratio. i had a very substantial drop off in yield. but i was tickled pink to get my amount of grain use down by half.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: coversall] * 1
    #27073193 - 12/05/20 09:22 AM (3 years, 1 month ago)

No not Necessarily pins only abort when they get wet then get fully dry so long as your tub never dries out and the pins always stay glistening they will not abort


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Edited by Jakeoncid419 (12/05/20 09:29 AM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27073196 - 12/05/20 09:23 AM (3 years, 1 month ago)

Yeah it will work but you are increasing your Potential for contamination without any benefit in terms of yield


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27073203 - 12/05/20 09:29 AM (3 years, 1 month ago)

Cool, that's a relief, nothing in my tub is getting fully dry anytime soon. I'm thinking about running the fans more often. If things are staying wet in there and the temps stay ok there's no reason not to run the fan more right?


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Edited by coversall (12/05/20 09:31 AM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: coversall]
    #27073204 - 12/05/20 09:29 AM (3 years, 1 month ago)

Right


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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #27073206 - 12/05/20 09:29 AM (3 years, 1 month ago)

his fc is very similar to mine actually click the link in ny sig if u wanna see it. but i have no issue at all with dripping from the top


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27073209 - 12/05/20 09:30 AM (3 years, 1 month ago)

Just keep a close eye on it when you increase the fan it only takes a short amount of time being too dry for everything to fry


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27073214 - 12/05/20 09:33 AM (3 years, 1 month ago)

Quote:

jcm4620 said:
his fc is very similar to mine actually click the link in ny sig if u wanna see it. but i have no issue at all with dripping from the top




Yeah I followed your post more than his, but I used to PCs like he did, mostly because I had some spare and wanted to use them!

I saw you've got loads of holes in your lid. I'm tempted to do that too.

Quote:

Jakeoncid419 said:
Just keep a close eye on it when you increase the fan it only takes a short amount of time being too dry for everything to fry




Yeah the timer plug I have right now only does 15 min incriments, but I've got one coming that lets me do much smaller ones. I'll feel more comfortable having it coming on more often for shorter bursts than 15 mins.

Before I setup the tub the FC I was using was doing a very poor job of maintaing temp, so it definetly dropped down to 14c one night. I'm keeping the trays going till my other stuff is ready to lay out, but am worried that temp drop will have ruined my chance of fruit.


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..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


Edited by coversall (12/05/20 09:36 AM)


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Re: Official Copelandia/Panaeolus Thread [Re: gt40]
    #27073220 - 12/05/20 09:40 AM (3 years, 1 month ago)

Quote:

gt40 said:
Quote:

p9hu7 said:
Pretty sure they have grains in their sub.



angam didn't. he did spore syringe to manure only as I remember.
It works for him, but things just go super random each time with that approach I guess. Also I believe gentamicin sulfate was involved ) It's super common for his area.




They can grow on sterilized substrate, they can grow on coir/straw. Im not sure that we can trust his terminology anyways, there is always something lost in his translation. There is a difference between coir/straw and pasteurized manure, the difference will most certainly be microbial.

Cubes seem to not be as finicky in regards to the microbes present in the sub, they grow equally well on coir and manure.


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Re: Official Copelandia/Panaeolus Thread [Re: Stipe-n Cap] * 1
    #27073233 - 12/05/20 09:52 AM (3 years, 1 month ago)

I mean pans and cubes and everything else doesn’t need grain to grow in the wild. Are wild mushrooms getting some other sort of supplementation when they grow in the wild. Feeding off of decaying matter for the saprobic I suppose. I really should do more research. I mean when you find a pan growing in cow shit in a field the cow shit isn’t mixed with grain so they are growing on straight cow shit. It obviously helps to have that little bit of outside supplement though just looking at everyone’s grows but at the same time it’s not necessary.


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Edited by Celestialexplorer1 (12/05/20 09:54 AM)


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