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InvisibleWall.E
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Re: Official Copelandia/Panaeolus Thread [Re: Pastywhyte]
    #27052065 - 11/22/20 12:11 PM (3 years, 2 months ago)

Even more a testament to jcm lol. Not to shit on you or anyone else but he seems to get good flushes from them but I know he takes a different approach with his clone hunting.

On another note, I have 6 jars that all look like the pictures and have the same smell, one has a sweeter aroma so I'm tossing that one, but it smells like what it's supposed to I guess. Like almond flavored earth I guess. Idk, I have a bag of shit and straw pasteurizing right now


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: Wall.E]
    #27052340 - 11/22/20 02:12 PM (3 years, 2 months ago)

That’s about how my cambo jam looked before casing. Super fast and super thick.

I wish I had all the pics I deleted to make room a while back still. This is the only one I can find and I think it was two or three days after spawning.



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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #27052350 - 11/22/20 02:18 PM (3 years, 2 months ago)

Ime overlay is all genetics, just bad luck I just go back to print and hunt again, for the fastest most wispy growth I can find


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27052352 - 11/22/20 02:19 PM (3 years, 2 months ago)

I haven’t had anything overlay in over six months though it could just be luck or I could have slowly bread those traits out of my genetics


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Edited by Jakeoncid419 (11/22/20 02:20 PM)


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InvisiblePastywhyteMDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #27052362 - 11/22/20 02:30 PM (3 years, 2 months ago)

I agree overlay is likely genetic but it’s probable IMO that the genetic component represents a threshold where a specific attribute (overlay) may dominate dependent on other factors (like having a sufficient consolidation period or how high CO2 levels are allowed to spike). I could just be full of crap tho.


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Invisibleelpico
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Re: Official Copelandia/Panaeolus Thread [Re: Pastywhyte] * 4
    #27052936 - 11/22/20 08:36 PM (3 years, 2 months ago)

Greetings, fellow pan-ites.  The contributions on this thread have really helped me to up my pan game, so thanks to all.  I felt compelled to share something really strange that happened recently.
  So I collected P. cyanescens a few years back in a pasture in Colima, Mexico.  I took several prints off several collections throughout this area.  I've been working off of one print for the last couple years, and have some really nice clones that I've isolated.  Here's a second flush from an F1 clone:


Now, recently I thought it would be fun to go back to a different print and see what kind of diversity I might find.  I swabbed the old print, rehydrated it for a week in dH20, and shoved it into soft agar.  I got aggressive germintaion after just two days and subcloned to three plates.  Each MS subculture(T1) was tested in  separate tray.  To my astonishment, each of the three trays put out completely albino, sporeless fruits:


I've taken several clones of these spore-less fruits.  So, what exactly is going on here?


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OfflineGeinstein Happy Birthday!
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Re: Official Copelandia/Panaeolus Thread [Re: Pastywhyte]
    #27053079 - 11/22/20 09:50 PM (3 years, 2 months ago)

Quote:

Pastywhyte said:
I agree overlay is likely genetic but it’s probable IMO that the genetic component represents a threshold where a specific attribute (overlay) may dominate dependent on other factors (like having a sufficient consolidation period or how high CO2 levels are allowed to spike). I could just be full of crap tho.




--Not Pan related--

I don't know how many of you have given NSS a shot before, but basically this cub always overlays with large clumps.
We send a couple of these overlay clumps to the university to be placed under microscope and found that the overlay everyone(16+ people I know personal) was getting wasn't overlay at all but rather another fungi(I'll recheck my notes because I forgot its name)

So we figured that NSS are prone to this particular fungi

The first one who has gotten around the NSS overlay problem did the following
Complete darkness well colonizing the sub & first two days of fruiting also in darkness

I colonized in light but just flipped the sub upsidedown when going into fruiting to get around the overlay


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InvisibleMateja
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Re: Official Copelandia/Panaeolus Thread [Re: elpico]
    #27053103 - 11/22/20 09:57 PM (3 years, 2 months ago)

Quote:

elpico said:
Greetings, fellow pan-ites.  The contributions on this thread have really helped me to up my pan game, so thanks to all.  I felt compelled to share something really strange that happened recently.
  So I collected P. cyanescens a few years back in a pasture in Colima, Mexico.  I took several prints off several collections throughout this area.  I've been working off of one print for the last couple years, and have some really nice clones that I've isolated.  Here's a second flush from an F1 clone:


Now, recently I thought it would be fun to go back to a different print and see what kind of diversity I might find.  I swabbed the old print, rehydrated it for a week in dH20, and shoved it into soft agar.  I got aggressive germintaion after just two days and subcloned to three plates.  Each MS subculture(T1) was tested in  separate tray.  To my astonishment, each of the three trays put out completely albino, sporeless fruits:


I've taken several clones of these spore-less fruits.  So, what exactly is going on here?



This is interesting :takingnotes:
I don't have experience with saving genetics or propagating/germinating albino spores but intuitively I feel like saying please clone and save many of those in culture slants for long term storage whatever happens :eatingpopcorn: (whatever they are sporeless or albino)


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InvisibleModularMind
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Re: Official Copelandia/Panaeolus Thread [Re: elpico]
    #27053179 - 11/22/20 10:20 PM (3 years, 2 months ago)

Quote:

elpico said:

I've taken several clones of these spore-less fruits.  So, what exactly is going on here?




Are you certain there are not imperceptible spores?


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InvisibleMateja
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Re: Official Copelandia/Panaeolus Thread [Re: ModularMind]
    #27053193 - 11/22/20 10:39 PM (3 years, 2 months ago)

I bought these two products, one is straw and the other is hay, what can you tell me about these as a substrate?



Also what is the consensus on growing Pans without poop? I was thinking of making a straw/coir/grain based substrate for Pan Cakes right now just to see what happens.


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Invisibleelpico
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Re: Official Copelandia/Panaeolus Thread [Re: ModularMind]
    #27053212 - 11/22/20 11:16 PM (3 years, 2 months ago)

Good point, I shouldn't state at this point that they are 100% spore-less.  I do not have a microscope available to look at the gills.  Let's just say that, after 48 hours, there is no spore deposit on foil from any of the numerous caps I attempted to print.  Also no visible evidence of pigmented spores on any of the mature gills or stipes just below the gills.


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: elpico]
    #27053223 - 11/22/20 11:31 PM (3 years, 2 months ago)

Mateah, I think Nobler Hino has done a few straw only grows.

I'm going the other way lately. Just poop and verm. The poo seems to have
enough straw in it already.


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: Mateja]
    #27053268 - 11/23/20 01:11 AM (3 years, 2 months ago)

Quote:

Mateah said:
I bought these two products, one is straw and the other is hay, what can you tell me about these as a substrate?



Also what is the consensus on growing Pans without poop? I was thinking of making a straw/coir/grain based substrate for Pan Cakes right now just to see what happens.




JOCs Tek has much less hpoo in it ~30-35% iirc. I'm trying my grains and LC on a sub with ~10% in, but the manure is from pellets which I've read are stronger?

I'll put some of my mother grains to a hpooless mix, they should be ready soon.

Why the coir in the straw mix?


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Offlinegt40
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Re: Official Copelandia/Panaeolus Thread [Re: Geinstein]
    #27053283 - 11/23/20 02:07 AM (3 years, 2 months ago)

Quote:

Geinstein said:
Quote:

Pastywhyte said:
I agree overlay is likely genetic but it’s probable IMO that the genetic component represents a threshold where a specific attribute (overlay) may dominate dependent on other factors (like having a sufficient consolidation period or how high CO2 levels are allowed to spike). I could just be full of crap tho.




--Not Pan related--

I don't know how many of you have given NSS a shot before, but basically this cub always overlays with large clumps.
We send a couple of these overlay clumps to the university to be placed under microscope and found that the overlay everyone(16+ people I know personal) was getting wasn't overlay at all but rather another fungi(I'll recheck my notes because I forgot its name)

So we figured that NSS are prone to this particular fungi

The first one who has gotten around the NSS overlay problem did the following
Complete darkness well colonizing the sub & first two days of fruiting also in darkness

I colonized in light but just flipped the sub upsidedown when going into fruiting to get around the overlay



wow


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Invisiblecoversall
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Re: Official Copelandia/Panaeolus Thread [Re: gt40]
    #27053287 - 11/23/20 02:13 AM (3 years, 2 months ago)

Angams grows are on just a hay/manure mix. Over in general cult someone said hay still as grains in it?


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: coversall]
    #27053314 - 11/23/20 03:23 AM (3 years, 2 months ago)

Those albinos are so sick. You should definitely try and stabilize them.


I’ve also never used straw, Idk what exactly is in my composted steer manure but it’s definitely got a lot of plant type stuff in there and my results have been fine.


Ok so these Aussies didn’t seem to want to colonize the sub that much but absolutely devoured the casing. This is about two days after casing.



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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #27053323 - 11/23/20 03:47 AM (3 years, 2 months ago)

aka, what is the substrate mixture you use for that steer manure? 50% manure / 50% verm? (by weight?)


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InvisibleMateja
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Re: Official Copelandia/Panaeolus Thread [Re: coversall] * 1
    #27053327 - 11/23/20 04:12 AM (3 years, 2 months ago)

Quote:

Asura said:
Mateah, I think Nobler Hino has done a few straw only grows.



:hatsoff:


Quote:

coversall said:
Why the coir in the straw mix?



No specific reason other than to help in creating a suitable substrate, I wasn't sure at first how much water the hay and straw would hold. I mixed equal parts rye/coir/straw, 0.5L hydrated rye, 0.5L hydrated coir and 20g of dried straw (which amounted to roughly 0.5L as well) to my surprise I found out that straw holds pretty much water. 20g dry straw weighed 125g hydrated, and 20g dry hay weighed 98g hydrated. I made 4 cakes and a qt jar of each recipe and also one qt jar of just hydrated straw to see how myc will colonize it all.



I also ended up making 2 different straw broths to test as LC and 2 different hay broths as well. I did this to gauge how nutritious these could be on their own as substrates. What I was surprised by was how dark the boil off water was from the straw and hay, lots of stuff leeching out after a quick boil. Here's a pic of all three boil off waters, first is made from 1L dry rye that was boiled for 45min and the other 2 came from boiling 20g dry straw/hay for 5 min in 1.5L water.


Each of these boil offs was diluted 20X and 40X and will be inoculated with a Pan LC when the broths cool.


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: Mateja]
    #27053338 - 11/23/20 04:28 AM (3 years, 2 months ago)

I always just eyeball stuff but probably about a third verm.


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #27053349 - 11/23/20 04:37 AM (3 years, 2 months ago)

you mean a third by volume?


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