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InvisibleJHOVA
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26971649 - 10/06/20 07:14 AM (3 years, 3 months ago)

Whats the average yield off one tray over 3 flushes dry?


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26971652 - 10/06/20 07:17 AM (3 years, 3 months ago)

Once you try out LC your never gonna wanna go back to wedges. Or at least I didnt. Itโ€™s so simple if you do it right in sterile conditions and procedures and so fast it will make wedges look primitive. Also I store them in a mini fridge and have taken 6-8 month old cultures out that havenโ€™t lost vigor at all. I believe when there dry for prolonged periods thatโ€™s when they start to loose vigor which can happen with stored plates sometimes. Not with slants so much. But I suppose if anything is properly stored it will retain its moisture.


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Edited by Celestialexplorer1 (10/06/20 07:20 AM)


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OfflineLuv_The_Cyans
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26971703 - 10/06/20 08:12 AM (3 years, 3 months ago)

Hey Panfam!
Looks like everyone is doing well! I have had a recent pandemic of trich :frown: Even after a full scrubdown of the lab. I took in a fruiting APE shoebox in my Jartha from a friend a couple months ago the turned green and I have been getting outbreaks since. I was hoping it would calm down when temps dropped(it has) but it is still there. Cubes are even turning green after a flush(never had that happen). Have to go to Mexico for business in 3 weeks so I am going to wait till I get back and start fresh. I hope Rain Euc is working out for some of you and I love seeing everyone's success and experiments on this thread. Keep on Panning for gold! :rockon:


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Invisibledfwerydfhg
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Re: Official Copelandia/Panaeolus Thread [Re: Luv_The_Cyans]
    #26971989 - 10/06/20 11:38 AM (3 years, 3 months ago)

Should pan myc grow faster on plates than cubes? Everybody always describes it as fast.

I put pan cambo spores to agar last week, made T1s at the same time as some cubes, and the cubes are growing faster...

It could be I got some shitty strains, I only had about 7 points of growth across two germ plates. Alternately, it could be temp? The room can get down to like 68 ยฐF, probably lower overnight. If it would help I could rig up a little incubator situation for the pan plates?


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Invisiblexspak
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #26972037 - 10/06/20 12:04 PM (3 years, 3 months ago)

Quote:

PsiloPsychIn said:
Xspak
What is your gas exchange set up on those jars?
If itโ€™s mycelium and stalled some times than an be a build up of CO2
If you have a good gas exchange set up you can rule that out...  ๐Ÿคทโ€โ™‚๏ธ




Hello PsiloPsychIn ... thank you for your reply indeed. And congratulations on your dark capped PC-mushrooms, they look beautiful.

I use two different kinds of lids. The ones that I make myself with a little metal tube grommet thing that I solder into the hole of the jar caps and then stuff tightly with a very fine polyfill (finer than the stuff you would use on a tub). These served me very well since years for cubes and sclerotia-producers. The grommet prolongs the way the air has to travel, hoping it helps keeping it cleaner. I get the grommets for free - before I came to use them I just did a hole in the caps and stuffed it with polyfill.
Sometimes I put a layer of micropore over it just to be sure.
I never had trouble with these lids so far, but I'm new to pans.

The other sort I use here right now is bought ready made. It are 0.2 micrometer filter disks built into GL45 lids of the bottles used my many to pour agar. They work very well, too. But only if the spawn is dry enough to get well shaken because of the bottleneck. I would not use these with stone producers. However, they should have a very good filtered air supply to the jar.

Pictures:






The pans I have problems with now are in both of the jars I hitherto described. In the same jars I have grains that work. These are on the fonio millet and the canary grass seed. They have optimal moisture.

The rye which I have prepared very very much on the dry side seems not helpful. Could too dry rye be a problem ? The difference between the ones that work and the ones that stalled are the grains and possibly genetics as they come from different petri plates.

It is always very hard to analyze over the internet :frown: Every help I am thankful for and I will try more until I manage it.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: dfwerydfhg]
    #26972051 - 10/06/20 12:08 PM (3 years, 3 months ago)

Quote:

dfwerydfhg said:
Should pan myc grow faster on plates than cubes? Everybody always describes it as fast.

I put pan cambo spores to agar last week, made T1s at the same time as some cubes, and the cubes are growing faster...

It could be I got some shitty strains, I only had about 7 points of growth across two germ plates. Alternately, it could be temp? The room can get down to like 68 ยฐF, probably lower overnight. If it would help I could rig up a little incubator situation for the pan plates?



Yes a quality pan culture should eat a dish in 7-10 days


--------------------
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OfflineDonkeyHodie
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Re: Official Copelandia/Panaeolus Thread [Re: JHOVA]
    #26972090 - 10/06/20 12:26 PM (3 years, 3 months ago)

Quote:

JHOVA said:
Whats the average yield off one tray over 3 flushes dry?




Depends on the size of the tray I guess. Yields are about a third that of cubes so probably somewhere in the range of 10g per quart of spawn.

I can't speak from experience though. I'm just now getting into pans myself, but I have read the first 1600 and last 1600 posts on this thread.

Jesus what have I done with my life.  :tearchalice:


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Invisiblexspak
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Re: Official Copelandia/Panaeolus Thread [Re: dfwerydfhg]
    #26972100 - 10/06/20 12:31 PM (3 years, 3 months ago)

Quote:

dfwerydfhg said:
Should pan myc grow faster on plates than cubes? Everybody always describes it as fast.

I put pan cambo spores to agar last week, made T1s at the same time as some cubes, and the cubes are growing faster...

It could be I got some shitty strains, I only had about 7 points of growth across two germ plates. Alternately, it could be temp? The room can get down to like 68 ยฐF, probably lower overnight. If it would help I could rig up a little incubator situation for the pan plates?




I have made the same experience, that with how I do it, they did not grow faster. One thing I learned so far that I try to keep them warmer, like 25ยฐC and keep the warmth constantly.

I think I observed so far that they are not so tolerant to temperature changes as cubes are.

Also, old spore prints seem to be problematic more than with cubes. I have no trouble germinating years old cube prints but with pans they seem to not work after 2 years already.

Also I think that they do not like my food coloring, which contains preservatives that work anti-fungal. So I will not use that anymore.

But please notice that with pans I am a beginner - so my observations might well mean nothing. What I do notice for sure is that stuff that works well for cubes does not get me very far with pans yet. I cannot put my finger on what exactly is the problem.

To make it work I try to make it as easy and fail-proof as possible in the future attempts.

Meaning I in the future try to keep a constant warm temperature.

And nothing in the agar that might be a problem and definitely is not necessary (from what I can tell, all the master pan growers in this thread use non-colored agar).


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Invisiblexspak
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26972107 - 10/06/20 12:35 PM (3 years, 3 months ago)

Quote:

Jakeoncid419 said:
Quote:

dfwerydfhg said:
Should pan myc grow faster on plates than cubes? Everybody always describes it as fast.

I put pan cambo spores to agar last week, made T1s at the same time as some cubes, and the cubes are growing faster...

It could be I got some shitty strains, I only had about 7 points of growth across two germ plates. Alternately, it could be temp? The room can get down to like 68 ยฐF, probably lower overnight. If it would help I could rig up a little incubator situation for the pan plates?



Yes a quality pan culture should eat a dish in 7-10 days




Hello Jake,

what can be the reason for this:

I made 5 dishes from spores. 2 of these looked too dirty. From the 3 left I made 20 new dishes.

None of them grow as fast as people say they should.

Is my spore print not good to begin with ? My agar recipe ? Temperature ? What could be possible reasons ... When I have all possible causes on the table I can try to eliminate one by one.


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Invisiblexspak
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Re: Official Copelandia/Panaeolus Thread [Re: xspak]
    #26972122 - 10/06/20 12:40 PM (3 years, 3 months ago)

Jake, another question if I may:

Everyone says not to make too many transfers with pans on dishes.

How do we get a perfectly clean dish with very few transfers ?

All my prints are on paper and have normal cleanliness, not perfectly clean but good enough if I can make a few transfers.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: xspak]
    #26972123 - 10/06/20 12:40 PM (3 years, 3 months ago)

Nope just bad luck of the draw on culture selection, go back to print spread your spores out on the dish and look for the fastest moving sections to transfer out


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Edited by Jakeoncid419 (10/06/20 12:50 PM)


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OfflinePuduwoke
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26972132 - 10/06/20 12:45 PM (3 years, 3 months ago)

Quote:

Jakeoncid419 said:
Quote:

dfwerydfhg said:
Should pan myc grow faster on plates than cubes? Everybody always describes it as fast.

I put pan cambo spores to agar last week, made T1s at the same time as some cubes, and the cubes are growing faster...

It could be I got some shitty strains, I only had about 7 points of growth across two germ plates. Alternately, it could be temp? The room can get down to like 68 ยฐF, probably lower overnight. If it would help I could rig up a little incubator situation for the pan plates?



Yes a quality pan culture should eat a dish in 7-10 days




At what specific temps Jake? 75-80F?:takingnotes:

I always had the same experience. That my Pan plates grow not as fast as other people. Had what I consider pretty good success with them tho:shrug:

Have to be honest tho.. when working with most cultures I don't really know what to look for. I know it takes a lot of practice tho and I do grow quite a few actives and different gourmets all for fun:stoned:

Pictures of my first Pan Alabama, Jamaica and Pan Trop coming with in 4 days. Because pins are in all trays now :leocheers:

Pan trop pins look tiny and very thin tho.. we will see.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Puduwoke]
    #26972141 - 10/06/20 12:50 PM (3 years, 3 months ago)

Yup usually around 78F


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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26972149 - 10/06/20 12:53 PM (3 years, 3 months ago)

Who says not to make too many transfers? I transferred cultures for months looking for different phenoโ€™s to clone. I hate paper prints lol, but Iโ€™ve never had issues with transferring pans multiple times.


--------------------
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InvisibleJHOVA
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Re: Official Copelandia/Panaeolus Thread [Re: DonkeyHodie]
    #26972234 - 10/06/20 01:56 PM (3 years, 3 months ago)

Quote:

DonkeyHodie said:
Quote:

JHOVA said:
Whats the average yield off one tray over 3 flushes dry?




Depends on the size of the tray I guess. Yields are about a third that of cubes so probably somewhere in the range of 10g per quart of spawn.

I can't speak from experience though. I'm just now getting into pans myself, but I have read the first 1600 and last 1600 posts on this thread.

Jesus what have I done with my life.  :tearchalice:




Quote:

Peacefrog said:
Yield was very good for pans. I use a mini tub ~13X9 inches or so. I like the smaller ones for pans personally. And it is a known tested isolate that performs well. Here was my dried yield after 3 flushes:


The agar work does not represent a pure culture, as there is still some sectoring going on, but it is the best pics I have of pan myc. to date. Those plates are anywhere from 5-8 transfers. They needed a few more to get a mono culture. It can take several transfers to achieve a pure culture IME.

They were as potent as any pan I have grown or tried. They all seem about the same to me. VERY potent indeed. Their potency makes up for their small stature and smaller yields verses cubes. But IMO, they are SO much better and cleaner than cubes.




Something like this is average yield? 3 flushes 35 grams dry seems pretty good.


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OfflineDonkeyHodie
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Re: Official Copelandia/Panaeolus Thread [Re: JHOVA]
    #26972258 - 10/06/20 02:11 PM (3 years, 3 months ago)

Quote:

d0urd3n said:
122 wet grams minus the 17 fat caps that were growing in clusters that are printing and the tiny ones that I ate :lol:

Super happy with the results for 1 qt of spawn!






Sounds like he got 13+ grams from a quart of spawn. Typical spawn ratios seem to be 1 spawn to 2 bulk sub, so a 3 quart tray. I think that was just his first flush.


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OfflineDonkeyHodie
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Re: Official Copelandia/Panaeolus Thread [Re: JHOVA]
    #26972292 - 10/06/20 02:30 PM (3 years, 3 months ago)

Quote:

Jakeoncid419 said:
Quote:

Asura said:
Thanks for clarifying. I guess with cubes people do just that...shoot for 1oz per quart. With pans you aren't going
to get those weights, but the potency more than makes up for that.



Well shot if ppl are only getting 1oz per quart of cubes then pans are much more profitable because I quite consistently pull aprox 3 oz off a quart ( although with pans I donโ€™t think the amount of spawn directly correlates to your yield.




A grams per quart of grain spawn goal may not apply as much as it does to cubes spawned to coir/verm since the substrate, or at least manure and straw, also contribute, which coir does not.

Either way, pans seem to produce as much or more psilocin per unit volume of spawn + substrate as cubes do, as far as I can tell.


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Invisiblexspak
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26972325 - 10/06/20 02:51 PM (3 years, 3 months ago)

Quote:

Jakeoncid419 said:
Who says not to make too many transfers? I transferred cultures for months looking for different phenoโ€™s to clone. I hate paper prints lol, but Iโ€™ve never had issues with transferring pans multiple times.




This is very good to know ! There are two very highly regarded threads here where it is mentioned to not go past T3. I would not find it polite to name names.

Thank you very much indeed for acknowledging that transferring should not be a problem. That way I can explore my cultures on agar to find something that looks and feels right.


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Re: Official Copelandia/Panaeolus Thread [Re: xspak] * 1
    #26972396 - 10/06/20 03:35 PM (3 years, 3 months ago)

My last shoebox I used a little less than half a quart of spawn and ended up with like 20g dry over a bunch of flushes.


The spawn goes a lot further with pans. Plus if you equate a gram of pans with an eighth of cubes that yield comes out to like 4-5 ounces per quart of spawn if it was cubes.

I know some of the jambo grows had ridiculous yields.


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Edited by A.k.a (10/06/20 03:36 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a] * 1
    #26972405 - 10/06/20 03:43 PM (3 years, 3 months ago)

60-80gs over 3 flushes is my iso average, but Iโ€™m looking for prints so I look at it more like 350-400 prints over 3 flushes


--------------------
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