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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #26970135 - 10/05/20 04:50 AM (3 years, 3 months ago)

what temp u keeping them at AKA?


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26970144 - 10/05/20 05:22 AM (3 years, 3 months ago)

Yeah I’ve had quite a few stone looking things too. The cambo made bigger ones and the cyan had a ton of tiny ones.


I just had them in a drawer at room temp. I never did run my Jambo clones I’m gonna go dig around and see if by some miracle any of the plates are still going.

This picture the plate is 2 months old



The other day I finally dropped Australian and rainbow eucalyptus to grain, hopefully they’re clean cuz I’m ready to run some more trays here.


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Edited by A.k.a (10/05/20 05:26 AM)


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Invisiblexspak
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26970445 - 10/05/20 11:20 AM (3 years, 3 months ago)

It is encouraging to see some success here.

Most my jars are looking not good. All the rye jars stall. The rye was made very much on the dry sinde though.

The jars with Canary Grass seed are doing better. Best do the ones with a Fonio-Millet, here is a picture after 9 days colonization:

On Rye - Stalled since shake 3 days ago:


On Fonio-Millet, 3 days after shake:


I would like to find out the reason. In your experience, what is the main reason for Pans to stall on grain ? Too wet or dry, contamination,  temperature ?

One Thing I think I might have noticed on agar, is that they seem to not like temperature fluctuation ? I never went below 21°C - but everything between 21 and 30 °C. Usually around 25°C, but there are fluctuations that I cannot yet control. I think that I noticed that after a larger temperature fluctuation the mycelium seems to stay undecided what to do next - stall or keep growing. So temperature fluctuation may be an issue ?

Next thing is contamination. While the agar looked fine when I inoculated the jars, the leftover as well as other plates that did not go into jars show some signs of possible bacteria.
Maybe I was just lucky that the jars with the Fonio and Canary grass did get the better plates ? I did not label every plate to every jar, so I do nor know exactly which plate made it into which jar.

The jar moisture. All the rye jars where very much on the dry side. For cubes it would have been good enough, but maybe not for pans ? The Fonio-Millet and the Canary-Grass jars where better moisture. Might this be my problem ?
The grain type should not be the problem. The smaller grains should be an advantage in colonization time.

But there is no reason except mistakes I made that caused my rye to stall. So I now need to find out what I did wrong.

I also noted, that some plates just stall. Others stalled, then started to look funny and now they grow again fast and good looking.

Here is a photo of one of these plates, T3 from Sept. 19th:


It grow fast until the point where it now looks darkish and flat. Then stopped growing for 2 or 3 days. Then continued fluffy and fast as if nothing happened. I also have some that seem to want to pin but nothing happens since days. So I think it is probably just bacteria.

What could be my main problems ?

Also with Agar: In all the tutorials I read it is said not to go many transfers and be on grain after 2, maximum 3 transfers - to ride the momentum. How do you manage to get clean cultures from prints with that few transfers ?

Thank you very much Jake. Are these plates normal ? Some of mine look similar after some time. Every more picture would be appreciated.

Quote:

Jakeoncid419 said:
Like this?




In your experience, what are the main causes of stalling on agar and grain ?


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OfflineNordel
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #26970488 - 10/05/20 11:54 AM (3 years, 3 months ago)

So here's a sad update regarding my wrongly cased (sphagnum peat instead of peat) panjam-tray:
9 days after i cased

Just white myc. And it has also some bubbly/foamy sections, which look rather strange and disgusting... :frown:

I'll try another tray with the correct peat variation...although i dont think that changes something.
Should i try to use a slightly thicker casing?
Just to counter the ravaging myc?


Edited by Nordel (10/05/20 11:55 AM)


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Nordel]
    #26970744 - 10/05/20 02:42 PM (3 years, 3 months ago)

that rye looks like mold to me


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Invisiblexspak
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26970829 - 10/05/20 03:48 PM (3 years, 3 months ago)

Quote:

jcm4620 said:
that rye looks like mold to me




Hi jcm, thank you for your quick reply. That one here ?



It is like that since 3+ days. If mold, I should have thought it would be green or some other mold spore color by now ?

I have 7 rye jars. They all stalled and I must figure out why. I have two jars with Fonio-Millet, thay seem to grow fine. Three jars with Canary grass seed. Growing, but not as good as the millet.

I tried to make better pictures so I can get help maybe.

On Rye (stalled or contaminated - has not changed at all since 3+ days, the agar was black, that dark thing is the wedge):


another stalled rye, also no change since days:


and another stalled rye:


On canary grass (still growing, but not very fast):

(the grayish stiff in the middle is of the agar, which was colored black with activated carbon (experiment from another thing I tried)).

On Fonio-Millet:


(please note that the black cavity in the fonio jar is a "hole" in the grains where the initial agar wedge is behind. The Fonio grain is so small that doesn't shake that well and quite a bit sticks to the first agar wedge - that how this hole came about after shaking).


Edited by xspak (10/05/20 03:49 PM)


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: Nordel]
    #26970976 - 10/05/20 05:33 PM (3 years, 3 months ago)

Quote:

Nordel said:
So here's a sad update regarding my wrongly cased (sphagnum peat instead of peat) panjam-tray:
9 days after i cased

Just white myc. And it has also some bubbly/foamy sections, which look rather strange and disgusting... :frown:

I'll try another tray with the correct peat variation...although i dont think that changes something.
Should i try to use a slightly thicker casing?
Just to counter the ravaging myc?




those bubbly/foamy sections look like mycogone (wet bubble). it likes to intertwine with mushroom mycelium on the casing layer. it's contam.
this thread has photos of wet bubble: https://www.shroomery.org/forums/showflat.php/Number/25285598

i think trying another tray with the correct "sphagnum peat moss" instead of "sphagnum moss" will help. but it sounds like you already know you chose the wrong casing material.

no, try a thinner casing. i hear letting the substrate consolidate for a day or two before you apply the casing layer helps with aggressive cultures but my pan experience is limited.


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: xspak]
    #26971184 - 10/05/20 07:49 PM (3 years, 3 months ago)

Xspak
What is your gas exchange set up on those jars?
If it’s mycelium and stalled some times than an be a build up of CO2
If you have a good gas exchange set up you can rule that out...  🤷‍♂️


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #26971187 - 10/05/20 07:51 PM (3 years, 3 months ago)

Has anyone seen pan cyan pins com in with very dark caps?
I’m trying to figure out if I should be happy or bummed because they already aborted...



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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #26971194 - 10/05/20 07:54 PM (3 years, 3 months ago)

have any of you pansexuals tried not adjusting the PH of your peat and verm casing? i've tried it both ways with cubes and haven't noticed a difference. :shrug:


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg]
    #26971200 - 10/05/20 07:57 PM (3 years, 3 months ago)

The ph of peat moss can vary bag to bag often needing very little adjustments So by not doing so you’re probably not off by a whole bunch anyway, But it’s always good to check because you never know when you’re going to get a batch that is way off and it fucks up everything you put it on


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26971212 - 10/05/20 08:02 PM (3 years, 3 months ago)

ok thanks good advice.

i wonder what the lowest PH peat moss that anyone has had success using without adjusting it.


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg]
    #26971242 - 10/05/20 08:16 PM (3 years, 3 months ago)

Dark pins are aborts psychin the casing looks kinda dry. That could be part of the issue.


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26971262 - 10/05/20 08:26 PM (3 years, 3 months ago)

Psilo, someone here had chocolate looking caps like that awhile
back but I can't remember who it was...and they came in from what
I remember. Puduwoke maybe?

Might be aborted, but you should know in 12 hours or so.


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26971289 - 10/05/20 08:44 PM (3 years, 3 months ago)

you really think that casing looks dry? :takingnotes:


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg]
    #26971307 - 10/05/20 08:51 PM (3 years, 3 months ago)

The small brown ones could be alright but the bigger dark dark blue one is an abort. And yeah I like the casing to be pretty wet.

Also what variety is that? If there cambos ive had cambos come in brown and turn light as they grow. But almost always when they get dark blue like that bigger one there goners


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Edited by Celestialexplorer1 (10/05/20 08:53 PM)


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1] * 1
    #26971499 - 10/06/20 01:59 AM (3 years, 3 months ago)

Wooot!

They are just dark pins... still growing and more on the way!
This is a multispore culture from a Pan Cyanescens Hawaiian - from under the rainbow eucalyptus...
courtesy of our friend LoveTheCyans!

Thank you Sir! I love the backstory on these genes man... I feel certain they will fuel the “set” with love and beauty.




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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #26971506 - 10/06/20 02:17 AM (3 years, 3 months ago)

Nice!

Yeah somebody had those black capped ones pop up a couple months ago.

I’ve said it a bunch but i guess it’s worth repeating for people starting out, pans thrive in conditions that would drown cubes. Don’t be nervous about soaking them, better to err on the side of too wet which isn’t really a thing. Too dry will insta kill them.

This is a sub that had flushed 4-5 times and I thought was done until I accidentally left the fog on super high and left for two days. Came back to a bunch more pins.


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OfflinePsiloPsychIn
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26971508 - 10/06/20 02:22 AM (3 years, 3 months ago)

L:ooking good!
I love a nice little surprise of unexpected fruits.
Are those Pan cambodginiensis?


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 2
    #26971511 - 10/06/20 02:30 AM (3 years, 3 months ago)

lol thanks yeah bonus pans are always good. Those were wild coast which are cyan I think, the most fused fruits ever. There was one with five stems and one cap in there.


I just shook my Australian jar hopefully I don’t run into the problems everybody seems to have with those.


I’ve still never made a pan lc, I think whatever my next one is I’m gonna try the lc to bag thing.


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Edited by A.k.a (10/06/20 02:33 AM)


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