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OfflineLieutenant Pan
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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26937516 - 09/15/20 06:37 PM (3 days, 7 hours ago)

Quote:

Asura said:
I'm not going to have time to prep sub for a few
days.




  I'll prep your sub if you give me a rating on here.


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Offlinefunchky
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Re: Official Copelandia/Panaeolus Thread [Re: Lieutenant Pan]
    #26937558 - 09/15/20 06:57 PM (3 days, 6 hours ago)

Strange problem with this tray. Caps are coming up looking rotted. Not sure what's causing it. Casing is not overly wet and they haven't been misted.has anyone seen anything like this before?





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OfflineA.k.a
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Re: Official Copelandia/Panaeolus Thread [Re: funchky]
    #26938334 - 09/16/20 04:49 AM (2 days, 20 hours ago)

Gotta be bacteria unless something in the sub is staining them.


Alright guys it’s that time again. I took two off the rainbow and I’m happy with them so far but the Australian doesn’t have any spots popping out at me.


I’m thinking the big circular colony towards the bottom left.

Any opinions?


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26938458 - 09/16/20 08:14 AM (2 days, 17 hours ago)

I’d give it a try it’s hard to tell when they just germinate what there gonna look like


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26938466 - 09/16/20 08:22 AM (2 days, 17 hours ago)


Thinking about picking the mature ones seeing as I’m just gonna print them


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Offlinexspak
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26938585 - 09/16/20 10:40 AM (2 days, 15 hours ago)

Hello all, a question.

I'm a little concerned about my current cultures. Would you say that about 1.5mm growth on agar at 23-24 °C is normal ? I expected it to be faster for Pans.

It is MS and 1st transfer from MS. They look healthy to me, but only grow about 1.5mm in 24 hours.

Agar is 12g LME + 5g oatmeal flakes (cooked and filtered) for 1 liter.


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: xspak] * 1
    #26938617 - 09/16/20 10:59 AM (2 days, 14 hours ago)

So the spore germinated in 24 hours or the first transfer has grown 1.5mm in 24 hrs. Either way that’s fast

24 hrs is questionably fast for spores to germinate. Also it takes awhile for a transfer to recover give it more than 24 hrs. 23-24 c is fine


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Edited by Celestialexplorer1 (09/16/20 11:01 AM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26938690 - 09/16/20 11:46 AM (2 days, 13 hours ago)

Here’s sum more pan TX (ms) got sum nice fruits for cloning but again with the sterile bulk I’m getting signs of contam before I harvest the first flush. I’m gonna try and go back to pasteurized bulk now that I’ve got more Myco time and I’m about replacing all my hood filters and clean rooms filters.



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Offlinexspak
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26938702 - 09/16/20 11:50 AM (2 days, 13 hours ago)

Oh, sorry, I wrote wrong. The spores took a couple days to germinate, like the first signs after 3 days.

And I revived a plate that originated from the same print that was in the fridge for about 16 months when I had to not enough time to study it until now.

So both the new germinated ones and the one revived grow about 1.5mm a day.

They were all started a week ago. Here is the one that comes from the transfer of the fridge plate:





The plate 16 months ago where this transfer is from looked quite dead, but I hope this is not mold I'm growing here.

Also asking because it is recommended to have the wispy and fast phenotype. It seems wispy but I don't know how fast they should be.


Edited by xspak (09/16/20 11:53 AM)


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: xspak]
    #26938817 - 09/16/20 01:07 PM (2 days, 12 hours ago)

It depends on a lot of things: the strain, nutrients used for agar. Sometimes different species like different nutrients or more or less. Sometimes you get a fast culture sometimes a slow one sometimes it performs poorly on agar but well on grain but usually if it’s slow on agar it will most likely be slow on grain too.


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InvisibleCHUCK.HNTR
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1] * 1
    #26938985 - 09/16/20 02:55 PM (2 days, 10 hours ago)

Hey pan thread,
excited to join the official thread.

Wanted to share these two pics of Pan.cy myc (my first cloned run), it's the second day since adding grain to bulk.

The grain sat for a month colonizing, and the myc seemed super slow and not very active. I mixed the bags a few times but it never recovered very well. I figured it was time to let if free on the bulk and it seems super happy to be jumping off the grain.




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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: CHUCK.HNTR]
    #26939137 - 09/16/20 04:02 PM (2 days, 9 hours ago)


Is there a good way anyone here knows to fight bacterial blotching on the caps.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26939229 - 09/16/20 04:52 PM (2 days, 8 hours ago)

phv and new rdu goin to grain tom👍👍 i cant fuckin wait to get some decent trays here man this last grow was a major let down


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26939459 - 09/16/20 07:08 PM (2 days, 6 hours ago)

Any idea why these pan cyans would be so short?


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1] * 1
    #26939502 - 09/16/20 07:26 PM (2 days, 6 hours ago)


More of the central flordia. Biggest caps I’ve had yet just below an inch an a quarter


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Edited by Celestialexplorer1 (09/16/20 07:27 PM)


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OfflineA.k.a
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26939562 - 09/16/20 07:57 PM (2 days, 5 hours ago)

Gotta be just short genes. Those caps are thick especially in the back, they look like they’ll drop monster prints.


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26939575 - 09/16/20 08:01 PM (2 days, 5 hours ago)

Attempted a clone on the big’n in the middle back you know the one. Inner stem sample. Not too good with pan clones we’ll see how it goes.


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OfflineA.k.a
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26939581 - 09/16/20 08:03 PM (2 days, 5 hours ago)

Lol yeah man i just accepted that I’m never getting a clean sample from a pan stem on the first plate. I’d need one of those jewelers magnifying lamp things and nerves of steel.


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26939602 - 09/16/20 08:16 PM (2 days, 5 hours ago)

Yeah and these stems are so thin I’ve tried peroxide wash and that never works at all for me either guess I’m in the same boat. Maybe an inner cap sample would be easier with these


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26939639 - 09/16/20 08:44 PM (2 days, 4 hours ago)

Damn Celestial, you have made a ton of progress. You gonna sample those soon?


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