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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Hereigrow]
#26888974 - 08/19/20 06:30 PM (3 years, 5 months ago) |
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If u made wash sheets I would
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Edited by Jakeoncid419 (08/19/20 06:41 PM)
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26888976 - 08/19/20 06:31 PM (3 years, 5 months ago) |
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You shouldn’t have much issue lining that up, that’s a good deal
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Hereigrow
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26888999 - 08/19/20 06:47 PM (3 years, 5 months ago) |
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I'm not sure what wash sheets are, but I do all mine in a stack of trays. My girlfriend is going to set up a vendor account over here this week and I think we're gonna kick things off with a big giveaway to get started and hopefully offload the bulk we have put back after we spread the love a little.
I also have 100+ each of APE, Melmac and Averys swabs
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Edited by Hereigrow (08/19/20 06:48 PM)
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Hereigrow] 1
#26889007 - 08/19/20 06:56 PM (3 years, 5 months ago) |
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So when I make syringes I wash spores from foil into a jar of sterile water with some antibiotic I raise my prints off the foil to reduce contamination the spores drop through the grid and land on the foil I can then squirt some sterile water scrape it around with a razor blade and then rinse it into my jar.
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Edited by Jakeoncid419 (08/19/20 06:56 PM)
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Hereigrow
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26889013 - 08/19/20 07:00 PM (3 years, 5 months ago) |
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You know I've seen that method before but I never did it that way because I assumed individual prints would be better, but this absolutely makes more sense for bulk. I think we will actually start doing this from now on. Not sure what a sheet would sell for though 😅
What's up with that APE jar? How'd you do that? My apes don't print like at all?
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Edited by Hereigrow (08/19/20 07:01 PM)
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Hereigrow]
#26889015 - 08/19/20 07:01 PM (3 years, 5 months ago) |
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They def sell for less than the ss’s but they are much easier to jam out.
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Hereigrow
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26889019 - 08/19/20 07:02 PM (3 years, 5 months ago) |
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Yeah I'll definitely be doing this from now on. I'll still make individual ones but it's pretty clear I don't need more than a hundred or so 🤷♂️
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A.k.a
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Re: Official Copelandia/Panaeolus Thread [Re: Hereigrow]
#26889029 - 08/19/20 07:09 PM (3 years, 5 months ago) |
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Do you let them print for like an hour first then move them to the big sheet so that the first layer of spores that’s most exposed to contams drops first and doesnt get mixed in??
That’s pretty cool, so when you need a syringe you can just draw some mixture from one of the jars real quick and be done?
Edited by A.k.a (08/19/20 07:12 PM)
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
#26889045 - 08/19/20 07:21 PM (3 years, 5 months ago) |
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Well no normally what I do is print them directly I just put them right on the grid, I haven’t had too many issues (as long as I keep them raised) and then I rinse into my jars I let that sit for a few days then I pull up a syringe and test it on grain and low nutrient agar once it passes the test I suck the entire jar up into the syringes and then unload them wholesale
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Edited by Jakeoncid419 (08/19/20 07:22 PM)
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Celestialexplorer1



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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26889068 - 08/19/20 07:41 PM (3 years, 5 months ago) |
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 Old wild coast plate I’ve been neglecting to throw out. Not sure if these are those “stones” everyone talks about
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Jakeoncid419
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Trash^
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Celestialexplorer1



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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26889088 - 08/19/20 07:51 PM (3 years, 5 months ago) |
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Already in there
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Hereigrow
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Hey I've got a couple wild coast plates getting all stoney too!

I'm doing a round of transfers in the morning and I plan on taking them to a couple new plates. Maybe I'll end up with blue springs and wild coast stones 🤷♂️ it seems like if you let them sit for about 3-4 weeks these little stone growths really kick into gear.
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Edited by Hereigrow (08/19/20 08:09 PM)
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Celestialexplorer1



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Re: Official Copelandia/Panaeolus Thread [Re: Hereigrow]
#26889110 - 08/19/20 08:11 PM (3 years, 5 months ago) |
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I’ve been finding the stones aren’t a good trait for fruiting strains In my limited experience I could be wrong though but there seems to be a correlation.
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Edited by Celestialexplorer1 (08/19/20 08:12 PM)
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Jakeoncid419
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I have never seen a pan stone in any of my pan dishes/grain/bulk/lc I’ve seen the pictures and that is kind of what they look like I just find it odd that with all the different pan species I’ve run I’ve never seen a single one has anyone ever grown out a culture with a stone in it and had it perform well? Has anyone collected worthwhile size stones out of the bulk or has it only ever appeared on agar?
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Jakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26889119 - 08/19/20 08:22 PM (3 years, 5 months ago) |
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Has in happened with cyans or only cints?
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Celestialexplorer1



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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
#26889128 - 08/19/20 08:29 PM (3 years, 5 months ago) |
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The only strain I have ever seen do that at all is tropicalis from India, wild coast and of corse cinctulus and I’ve had 13 strains of pan cyan 15 including the camboginiensis sandose and Goliath.
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A.k.a
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I got big ones from the cambo jams.
I cloned one, never got around to running it yet but the plates I put it on had a bunch of tiny pin clusters sprout up, it was pretty cool.
This was the first one, I think eventually there were three.

I really should pull that up and run it since it’s pinning like that on a plate.
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LAGM2020     
Edited by A.k.a (08/19/20 08:42 PM)
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Celestialexplorer1



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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
#26889206 - 08/19/20 09:30 PM (3 years, 5 months ago) |
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Didn’t see it with Bisporus either.
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Edited by Celestialexplorer1 (08/19/20 09:56 PM)
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Jakeoncid419
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Just hit my wife with her first agar class, she is a natural!
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