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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Supalemonhaze]
    #23229861 - 05/15/16 12:31 PM (7 years, 8 months ago)

Sorry if I was unclear- That first plate is after the agar sandwich (what was transferred from the sandwich) I did scrape from the top piece of the agar sandwich only.

Wow- you're pulling all the stops to get it to work- 10 plates! But the pic looked good? I don't get why its not growing in LC.

I'm learning Pans can be more mysterious than cubes.


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InvisibleSupalemonhaze
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #23229905 - 05/15/16 12:45 PM (7 years, 8 months ago)

Ah, my bad. Didn't enlarge the pic and the rectangle growth made my think that it's the sandwich.

The LC's are part of the reason why I'm suspicious of the plates. The one I posted does indeed look clean but yesterday I noticed a yellow hue on the mycelium so I could have mislabeled my galindoi. That would genuinely suck STD infested genitalia. I have a couple more plates with thick growth like yours, the type that you only see a blob of white with a light from the back. Who knows?

I got around 5 germination spots in one of my plates a couple days ago, transferred them all today but this plate has been there over two weeks before I warm poured so I'm not entirely sure if what is growing is pans. Germination spots seem to be around the edges as well, might just be that the warm pour pushed them there or I fucked up with the pour. I used a mason jar for the pour, not exactly what I'm accustomed to so it's entirely possible I fucked up. They are growing from beneath the surface at least, which seems to indicate that it is the spores rather than molds. Time will tell.

Another thing I'm suspicious of is that everyone says how fast pans are, my galindoi is growing faster than my "pans". Maybe they need to pick up some vigor due to being only a couple transfers old but I dunno.:shrug:


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Supalemonhaze]
    #23230037 - 05/15/16 01:29 PM (7 years, 8 months ago)

Regarding the "yellow hue", this is probably not the same thing but sometimes I see a light brown to almost yellow color on my older plates that have spent some time in the fridge- like the plate on the right here:


I'm assuming its caused by some aging or drying of the mycelium- at least I hope so!

I'm going to cut some more uniform sectors from my Hawaiian Y plate and see if i can get symmetrical  growth. I've been playing with this strain on agar on and off for 6 months.

Pan growth on agar- has been pretty variable for me- some fast -Jamaican and some slower- Chicon nindo, Hawaiian. Maybe it's just the luck of the spores but I suspect it also relates to the variety.


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InvisibleSupalemonhaze
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #23230095 - 05/15/16 01:49 PM (7 years, 8 months ago)

It does look like the ring in the 2nd picture, but mine is still very young. 2nd transfer from inoculation and about an inch in diameter. I don't normally mislabel plates but given the similarity in the names Galindoi and Goliath, I cannot be sure. Shit, I only got this vial because the vendor mistook the 2 :lol: I ordered Galindoi originally. Thankfully, I got a Galindoi print from a friend of mine.

If they sold prints instead of vials I'm sure I would be having much better luck. Spore solutions suck for agar.


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: Supalemonhaze]
    #23230813 - 05/15/16 05:23 PM (7 years, 8 months ago)

I dont trust vendor spore syringes- at least 1/3 of the time they're either contaminated with bacteria or mold and/or the spores have low viability or no viability. I've noticed this even more with Pans than cubes.

For me all vendor derived pan spores have to pass the agar test.


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InvisibleSupalemonhaze
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
    #23232324 - 05/16/16 02:38 AM (7 years, 8 months ago)

Nothing better than a print for agar, I agree. None of the european vendors have prints for the exotic species unfortunately so I had to make do with vials.

Vials are even worse than syringes really, I was sucking up some pan spore solution when I heard the port on the vial leak outside air into it's inside. Since they are air tight the pressure keeps dropping every time you  aspirate with the syringe so the smallest disruption on the port can cause it to suck in air from it's sides. I was in front of my flowhood but I bet something still made it inside it from the outside of the vial and syringe.


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Offlineralph larun
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Re: Official Copelandia/Panaeolus Thread [Re: Supalemonhaze]
    #23243261 - 05/18/16 07:31 PM (7 years, 8 months ago)

Got some sandose going on agar. Asked amu already but it a mostly dung sub the way to go with these still?


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OfflinePsilosoulful
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Re: Official Copelandia/Panaeolus Thread [Re: ralph larun]
    #23243378 - 05/18/16 08:11 PM (7 years, 8 months ago)

Yep, you want to use dried hpoo/chopped straw/gypsum for the best results IME
Although I've seen some pretty nice grows done off CVG.

Make sure to pasteurize the mix properly, that's a very important step!
And did I mention having the cleanest spawn possible?
That is what will make or break your grow, it really doesn't matter what you spawn to


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Offlineralph larun
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #23243665 - 05/18/16 09:26 PM (7 years, 8 months ago)

Okay sweet. I have cow poo right now and use cvg normally. Straw is a little hard for me to get with out a truck. I've been trying to get hpoo her but the stable workers turn me away when I ask.

But defently they are on there 3rd transfer now I'm going to transfer 2 more times to makes sure they are more then clean.


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Offlinecigam
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Re: Official Copelandia/Panaeolus Thread [Re: Psilosoulful]
    #23243680 - 05/18/16 09:33 PM (7 years, 8 months ago)

So what im wondering is how you guys work with, and or mix the vendor hpoo.
I just received some hpoo from one of the trusted vendors.
I have coir, verm, straw, gyp.
The poo is ready to use i'm assuming. So do i just pasteurize the straw, verm , gyp and after that just mix in the poo. Then mix with spawn grains?
I haven't opened the bag of poo yet. Is it at field cap, if not whats the technique.

BTW, yesterday i made up some hpoo, verm,coir,gyp with a little bit of hpoo i had collected. I wanted to try the stuff i collected and the vendor pre made stuff just to compare.
My first attempt with this type of substrate.


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InvisibleGrey
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Re: Official Copelandia/Panaeolus Thread [Re: cigam]
    #23243970 - 05/18/16 10:57 PM (7 years, 8 months ago)

Is the vendor stuff actually ready to use? Most require pasteurization still. Some may not. 

If it already is,  you can pasteurize the other stuff and mix together.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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InvisibleSpitballJedi
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Re: Official Copelandia/Panaeolus Thread [Re: Grey]
    #23244068 - 05/18/16 11:15 PM (7 years, 8 months ago)

If it's the dry pellets, it will need to be pasteurized.

I tried it back when I first started learning and failed miserably. Of course, my failures can easily be chalked up to lack of experience and I've recently considered g1ving it another go.

Pastywhyte says he's had success with it. It turns to mud if you just add water to it, so it's better to mix it while it's still dry. According to him, only use about 10%, or a little less, by volume. The vendor I got it from says to do 10% by dry weight. Either way, mixing dry manure pellets with field capacity substrate and then adjusting the water seems the way to go, but, however you do it, you need to pasteurize the manure pellets for sure.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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Re: Official Copelandia/Panaeolus Thread [Re: SpitballJedi]
    #23244145 - 05/18/16 11:34 PM (7 years, 8 months ago)

Here is what i bought. It says its been sterilized, and it comes in a bag with a filter patch.It looks like it is ready to be used.
I just want to make sure i use it wisely. Just wondering if anyone has used it and how.


Manure Based Mushroom Growing Substrate
Please note if you plan to start from a spore solution you need to begin with Grain Spawn.
Manure Based Mushroom Growing Substrate is a specially formulated mushroom growing substrate for growing gourmet edible mushrooms. We take field leached horse manure, shred it and then fortify it with all the necessary minerals and vitamins that mushrooms need to grow. We get our manure from one single source.
Manure Based Mushroom Growing Substrate should be used in conjunction with our mushroom grain spawn, rye berries and mushroom casing. You can mix and match our products to make your own personalized mushroom grow kit.

Manure Based Mushroom Growing Substrate comes to your front door sterilized and ready to use! You will receive your substrate in a heat sealed, large mushroom grow bags with a .05 µm filter patch to provide perfect fresh air exchange and filter out bacteria and other contaminants. We use the largest spawn bags available to insure plenty of room for substrate and the fruiting mushrooms. By cultivating in these mushroom grow bags you don't have to adjust humidity or worry about contaminants compromising your grow. This is real easy, even for beginners.


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InvisibleSpitballJedi
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Re: Official Copelandia/Panaeolus Thread [Re: cigam]
    #23244269 - 05/19/16 12:13 AM (7 years, 8 months ago)

Since it's sterilized, you will have to put your colonized grains in there in a SAB, in front of a flowhood, or some other sterile environment. In other words, if it is sterile, it will need to remain that way, not exposed to open air or non-sterile materials, until colonized.

What that means is, you aren't going to be able to mix it with your other substrate materials. Well, technically there is a way, but not practically. Once it's colonized, you can case it though.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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InvisibleSupalemonhaze
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Re: Official Copelandia/Panaeolus Thread [Re: SpitballJedi]
    #23244400 - 05/19/16 12:47 AM (7 years, 8 months ago)

Yeah, that is a bummer bag of poo. Get the dry one next time, it will be cheaper as an item because they haven't sterilized/pasteurized and shipping will be cheaper because you won't have to pay for the water weight. Or get the ones in agri shops, I hear they sell bags of pellet poo over there as well.


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Offlineralph larun
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Re: Official Copelandia/Panaeolus Thread [Re: Supalemonhaze]
    #23244464 - 05/19/16 01:08 AM (7 years, 8 months ago)

I would love to find a agri shop with dehydrated pellet poo. Because are vendor is expensive for 5 gallons.


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InvisibleGlitchInSpace
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Re: Official Copelandia/Panaeolus Thread [Re: ralph larun]
    #23245261 - 05/19/16 08:30 AM (7 years, 8 months ago)

check ebay, they have some good deals on manure.


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Offlinespacechildo
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Re: Official Copelandia/Panaeolus Thread [Re: GlitchInSpace]
    #23245466 - 05/19/16 10:03 AM (7 years, 8 months ago)

why would anyone BUY manure? get some straw instead, those vendors dont know what they're doing.
Or maybe they do know, but assume you dont know, it gets confusing, and wtf would they sterilize their manure??


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Invisiblethe_r3dz
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Re: Official Copelandia/Panaeolus Thread [Re: spacechildo]
    #23245472 - 05/19/16 10:05 AM (7 years, 8 months ago)

I think it looks bad on shroomery to have those types of vendors as sponsors.
Constantly people come here with grow kits that they bought from a sponsor, or something similar to this dude up here

then they come to the amu or make a new post and they all have to help them to grow mushrooms right, without the help of those scammy companies

why does shroomery support that?


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Invisiblemary fairchild
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Re: Official Copelandia/Panaeolus Thread [Re: cigam] * 2
    #23245548 - 05/19/16 10:35 AM (7 years, 8 months ago)

THATS THE SAME STUFF I USED- Its ready to go- didn't add anything. If its the 1lb bag it gives ~1300 to 1400 milliliters of dry volume. Its good for two~ 8"X 6" trays at  ~2:1 sub:spawn. Just my opinion- wouldn't add straw, at least the first time- Pans don't NEED straw to work and its a risk factor for a contam. Add it in a future grow to optimize. I saw no difference straw vs no straw.

Used this sub for 2 cube grows and one Pan grow-
I did NOT mix that STERILIZED sub under aseptic conditions, just took it right out of the bag in the open air and it worked great; it seems to get good reviews & I doubt that everyone else is mixing it with the spawn in a SAB. Wore gloves and used a clean plastic spoon to do the layering with spawn. Not to turn this into a rant against pasteurizing but it worked for me this way.


It came kinda dry- definitely less than RR's definition of field capacity- so I misted it just a bit when layering it with the spawn but it was still below field capacity- thats part of the reason I gave the colonized grains a brief water soak before layering.

Just made sure there were no air currents going on in the room when layering it. I've always layered rather than mixed -except when doing mono tubs- both ways seem to work for me.

I compared this sub to my own cow poo sub, which I also sterilized- both colonized well- no contams but my cow sub yielded way less fruit.

See the link under the mountain pic for full details.


Edited by mary fairchild (05/19/16 01:44 PM)


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