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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,005
Loc: The Island of Misfit Toys
Last seen: 3 months, 17 days
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Re: Official Copelandia/Panaeolus Thread [Re: SalviaGod]
#23143740 - 04/22/16 09:30 AM (7 years, 9 months ago) |
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Quote:
SalviaGod said: If i dont have H poo or any kind of poo whats the best way for me to grow Pan. Cambo sandose?
Store bought manure works fine. Check my signature.
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mary fairchild
Pantheist

Registered: 12/06/14
Posts: 777
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Pan cy Jamaican low tek grow [Re: MacMerdin] 3
#23166019 - 04/28/16 05:23 PM (7 years, 8 months ago) |
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Special thanks to the posters of the Official Panaeolus Strain thread!
I’ve been lurking this forum for several years now and at most have been gleaning info with the occasional question and no contributions.
It’s been said that there aren’t a lot of RECENT Pan cy teks out there. There is actually quite a bit of info on growing Pans in the Official Panaeolus Strain thread- which is recent, in fact it helped me do my first Pan cy grow. So here’s a contribution- Pan cy Jamaican from spore syringe ->product. I’m calling it low tech because the most high tech thing was a bit of agar work. No monotub, no liquid culture, just a SAB & SGFC. So its kinda noob friendly.
FIRST PAN CY GROW, VARIETY- JAMAICAN
SUMMARY: Spore syringe-> 4 passages on agar plates to select for robust uniform growth-> agar wedges to grain jars-> grains mixed with horse poo based sub in trays-> fruit in SGFC. BIOASSAY- POTENT, AT LEAST 3X> CUBENSIS, AND DIFFERENT - MORE LIKE LSD.
GERMINATING SPORES: Nocd Malt extract agar plate with 12 drops of spore suspension, Inc’d the plate at 75-80F. Note- spores didn’t want to germinate from just a couple of drops- so my plate was swimming in spores before I finally got germination; I dont remember- I think it took ~12 days to germinate. In hindsight there are ways to concentrate the spores into smaller volume- ie. let syringe sit needle point down for a couple hours -so the spores settle near the needle end of the syringe- before inoculating.
SELECTING FOR UNIFORM ROBUST GROWTH: After the starting plate (called the P0 plate) was ~3/4 colonized transferred 3 small wedges of growth (from the outer edge of the growth)- each to a new agar plate. Note- At this point the growth was so amorphous that I really wasn’t selecting for anything- just xfering new growth.
Let the 3 xfered agar wedges grow out till they covered ~ 3/4 of the plates. Called the P1A, B, C plates. These 3 plate yielded colonies that were more uniform (circular) but not completely circular. From these 3 plates was chosen the most uniform strong thick growth and took a single small wedge to a new plate- called the P2 plate. this procedure was repeated again to give a P3 plate:
 as you can see this growth is still kinda weird shaped so a small wedge from this plate (arrow) was xfered to a 4th plate which after grow out looked like this:
 This plate was used to noc. grains:
INOCULATING GRAIN The colony from the plate above was evenly divided into 4 wedges. Two wedges were dropped into two 1 pint rye berry jars- two wedges per jar. (rye berries were prepd. by RR’s procedure):
 Jars were inc’d @75F and at 10 days post inoculation looked like this:
 At this point jars were shook to evenly distribute the spawn. Recovery of growth was obvious by 12hrs. and colonization reached 100% by day 15. For no particular reason waited a full 17days before harvesting to mix with the substrate.

MIXING WITH SUBSTRATE IN TRAYS After breaking up the spawn in the jars THE SPAWN WAS SOAKED for 10 minutes in room temperature SPRING water, (hey, my tap water smells like chlorine), then strained the soak water out. Note: Interestingly - when pouring out the broken up grains prior to soaking there was a mushroom growing from one of the agar wedges that was used to inoculate the grain, so I cloned it; also the soak water turned a deep blue color after ~15’?yea!

The spawn does NOT smell like cubensis- but mild almondy- nice!
The substrate- A purchased Horse manure based sub that THEY SAY was sterilized- I DID NOT mix the spawn with the sub under aseptic conditions. Note- Still working on my own cow based sub- but that’s another story.
Some spawn: sub considerations: There is ~270ml of colonized grains in each 1pint grain jar, the trays are 8inch X 6inch X ~1.75 inch deep and hold ~1.1Liter total volume: So for one grain jar (270ml spawn) ~600ml of substrate was used to give~ a 2:1 ratio of sub to spawn and a total volume of 870ml- the calculations aren’t so important - just that it gave a ~2:1 sub: spawn ratio and the depth was <2inches.
Two trays were used - each received spawn from one 1pint grain jar and ~600ml of substrate: The soaked spawn was layered with the substrate in the trays until the trays were filled almost to the top. Trays were covered with foil and small slits were made (as per RR’s procedure). Trays were inc’d at ~77-81F and at 11days looked like this:
 At this point went to casing
CASING & FRUITING With 1:1 verm : peat and some CaCO3; just enough case to cover the cake (RR’s proc). Trays were left uncovered and put in the SGFC with periodic misting/ fanning,~77-82F. Note there were some- possibly important- misting/ fanning / humidity considerations I’d be glad to give if requested.
 First pinning by the end of 4 days under fruiting conditions, the casing layer never really "colonized":
 Later:
  CONCLUDE/YIELD: Little guys, a pain to harvest & clean but they dried super fast. Got 9.8g dry- from two 1pint jars (~540ml spawn) not much, but trip report (size doesn’t matter here!) is another story.
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Edited by mary fairchild (05/25/16 06:13 PM)
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Awesome job man, I'm a little jealous lol. You got me wanting to bust out my pan jam slant and give em another go
Edited by PinPornProducer (04/28/16 05:30 PM)
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Mr.PhilCybin
Master Baiter


Registered: 06/13/11
Posts: 11,642
Loc: Gnarnia
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beautiful man.
I'll be giving pans another go as soon as I get back from a business trip I'm on.
Pan cambo trays are ready to case as soon as I get home, and I'll get more cyans rolling again.
great grow man
-------------------- I'm stupid, Falcon91Wolvrn03 is smart. I'm ugly, Falcon91Wolvrn03 is beautiful. I'm a loser, Falcon91Wolvrn03 is a winner. Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.
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mary fairchild
Pantheist

Registered: 12/06/14
Posts: 777
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Re: Pan cy Jamaican low tek grow [Re: Mr.PhilCybin]
#23166179 - 04/28/16 06:02 PM (7 years, 8 months ago) |
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Thanx guys, Chicon Nindo and Hawaiian are in progress.
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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2x 2L poo bags and 6 pints on the go of Pan Camb 'Florida'
Hopefully will have some nice pics for May/June and clones/prints.
Nice addition to the thread Mary
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cigam



Registered: 11/20/14
Posts: 165
Loc: California
Last seen: 7 years, 2 months
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Mary,Nice post. I agree this is a good thread for learning about pans. I would like to hear your ideas on possibly important- misting/ fanning / humidity . Im in the middle of the agar stage and just reading as much as i can for the next steps. If po is the plate that gets inoculated with the syringe than i am on the p1 plate and this weekend i will transfer to p2 plate.
Edited by cigam (05/02/16 09:21 PM)
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mary fairchild
Pantheist

Registered: 12/06/14
Posts: 777
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Re: Pan cy Jamaican low tek grow [Re: cigam]
#23167891 - 04/29/16 04:01 AM (7 years, 8 months ago) |
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After reading about the environmental conditions that Pans "are supposed" to like I tried to replicate them. I don't really know if it was important to do what I did, but it worked- at least with this variety.
Went on the assumption that Pans are just more finicky than Cubes- so tried to give them more Fast air exchange and free air exchange and higher local humidity.
SGFC provided 4->5 inches of perlite in the SGFC and watered the perlite once or twice a week with a garden watering can. After watering the perlite id fluff it up to promote air movement thru it. I sat the whole SGFC on some soup cans so that it sat several inches above a boot tray to catch the runoff:
 I live in an arid climate and it was winter so the relative humidity in the grow room was ~25-30% but the humidity at the surface of the perlite ,using a calibrated hygrometer, was always between 92% and 99%(set the pet shop hygrometer on a piece of plastic grating on the surface of the perlite:
 MISTING- up to the point of pinning I only misted the surface of the trays when the casing started to look dry- this turned out to be only ~2X per day. After pins appeared I stopped misting and watered the surface around the pins with a syringe, a bit tedious but it worked ok. [BTW-its true- when I misted a few pins they tended to turn black and abort even if I fanned the hell out of them afterward to evaporate the water off of them.] Maybe in nature- outdoors when it rains on the Pans there is so much more air movement that prevents them from aborting.
FANNING- Trying to replicate an outdoor environment I fanned often using the cover to the SGFC ~ every 2 to 3 hours during the day but no fanning over night; this is more than I would fan cubensis- this would cause an immediate drop in humidity but the humidity recovered quickly.
AGAR WORK- I was just selecting for uniform growth. I didn't say this above but I also took the P3 plate to fruiting- this plate was less uniform in growth- it fruited, but gave only ~ 1/2 to 2/3 the product of the P4 plate. I'm surmising that different mycelia "species"- some that were inferior fruiting types- were competing with stronger fruiting forms. Again, I don't really know.
Anyway I'm learning as I play with this species. So far Ive also looked at Australian, Hawaiian and Chicon Nindo on agar, they all behave differently, maybe its just the luck of the spores.
What variety of Pans are you working with? Best of luck with your grow, i'd like to hear how it goes, keep this thread going!
Edited by mary fairchild (05/02/16 10:13 PM)
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Well done mary!
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FuckIDK


Registered: 02/01/16
Posts: 62
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Re: Pan cy Jamaican low tek grow [Re: d0urd3n]
#23181206 - 05/02/16 06:00 PM (7 years, 8 months ago) |
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What is the best was to grow pans?
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Pan cy Jamaican low tek grow [Re: FuckIDK] 2
#23181225 - 05/02/16 06:07 PM (7 years, 8 months ago) |
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reading this thread from the start is a good..start.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Pan cy Jamaican low tek grow [Re: Psilosopherr]
#23181899 - 05/02/16 08:40 PM (7 years, 8 months ago) |
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Pan cy Jamaican low tek grow [Re: Psilosoulful]
#23227870 - 05/14/16 10:19 PM (7 years, 8 months ago) |
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I just got access to a horse pasture today and have a Pan Cyan TX print on the way. I just wanted to be a part of this thread. I'll start reading it tomorrow. Looks informative.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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cirilo.h
local mycologist



Registered: 10/17/15
Posts: 180
Last seen: 4 years, 10 months
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im sittin on a bunch of prints ....this is motivating!
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cigam



Registered: 11/20/14
Posts: 165
Loc: California
Last seen: 7 years, 2 months
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Re: Official Copelandia/Panaeolus Thread [Re: cirilo.h]
#23228136 - 05/14/16 11:42 PM (7 years, 8 months ago) |
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I'm working on them also. This is what i have right now. I'm continuing to make transfers on agar and thought i would put a couple of the used cultures to grains , just to see what i get.
 
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Official Copelandia/Panaeolus Thread [Re: cigam]
#23228145 - 05/14/16 11:45 PM (7 years, 8 months ago) |
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Cigam, got any pics with the light through the back? I have some supposedly goliath mycelium, wish to compare it to something concrete.
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cigam



Registered: 11/20/14
Posts: 165
Loc: California
Last seen: 7 years, 2 months
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Re: Official Copelandia/Panaeolus Thread [Re: Supalemonhaze]
#23228361 - 05/15/16 01:07 AM (7 years, 8 months ago) |
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I'm still learning how to get good close ups. I am deleting more pics than i keep. I am also working with the Goliath strain. It is considered a strain , right ? or species ? I am still a beginner, how do they look?
these 3 pics are the same dish
  
this one is another dish
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Official Copelandia/Panaeolus Thread [Re: cigam]
#23228484 - 05/15/16 02:09 AM (7 years, 8 months ago) |
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I have one that looks similiar to those but I noticed a yellow-ish hue yesterday. I'm torn between thinking it's goliath or galindoi. Hope it's goliath, these spores are a bitch to germinate. It doesn't show in pics so I can't explain what I'm seeing any better.
 
Yours look good by the way, clean.
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mary fairchild
Pantheist

Registered: 12/06/14
Posts: 777
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Re: Official Copelandia/Panaeolus Thread [Re: Supalemonhaze]
#23229772 - 05/15/16 12:07 PM (7 years, 8 months ago) |
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Your goliath is nicely symmetrical- I inoculated goliath and sandose spores on to agar two days ago- how long did it take yours to germinate? I have 5 varieties of Pans on agar now
Can anyone help me with this hawaiian? the spore syringe came loaded with bacteria- and I've been trying to clean the mycelia by the "agar sandwich method"- these 3 colonies are supposedly cleaned but the growth is kinda weird shaped:
 transfer
 after grow-out
 same plate backlit
Took 3 wedges from the first plate and xfered to another plate- don't know if this amorphous growth is just sectoring or if it's sign of a contamination.
Any thoughts on where to go from here?
Edited by mary fairchild (05/15/16 12:15 PM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Official Copelandia/Panaeolus Thread [Re: mary fairchild]
#23229793 - 05/15/16 12:14 PM (7 years, 8 months ago) |
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You don't take wedges after an agar sandwich. You scrape the top of the upper piece of agar. When you made the sandwich, the bacteria can only access the lower part of the agar so under the sandwich is all filled with bacteria. The mycelium however can grow through agar so once it pokes out of the upper part, you scrape some agar off and put it on a new plate.
I don't actually remember how long it took me to germinate them, I do know that I recieved the vial about 3 weeks ago or so. It's been a hell of a ride, I'm still not sure if I actually have pan mycelium or not. I've tried close to 10 agar plate inoculations made with soft agar and eventually warm poured over. I also have 3 LCs that have been sitting there for a week without any germination which is strange because with LC you expect them to germinate fast. I'm gonna try a small grain jar next, almost out of shit to try.
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